Contents

Olympus DP23, DP28 Instruction Manual PDF

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Summary of Content for Olympus DP23, DP28 Instruction Manual PDF

Instruction Manual DP23 / DP28

Microscope Digital Camera

OpticalMicroscope Accessory English

Contents

1 Safety 4 1.1 Intended use 4 1.2 Avoiding personal injuryand device damage 4 1.3 Explanationof the symbols used 6

2 The DP23 / DP28 camera 7 2.1 Scope of supply 7 2.2 System diagram 8 2.3 LED status indication 9 2.4 Specifications 10 2.5 Operating and storage conditions 12

3 System environment 13 3.1 Microscope and camera adapter 13 3.2 Recommended computer and monitor configuration 14 3.3 Restrictions onuse 14

4 Assembly 17 4.1 Attaching the camera 17 4.2 Connecting the USB camera cable 18 4.3 Installing the software 19

5 Acquiring images 20

6 Cleaning the camera 21

7 Troubleshooting 22 7.1 Possible problems 22 7.2 Contacting Customer Service 26

8 Notes on conformity and disposal 27

About the DP23 /DP28 camera The DP23 /DP28 cameras are CMOS color cameras witha high frame rate. Theywere speciallydeveloped for acquisitions using light microscopy. The cameras canbe connected to all commontypes of light microscope bya C-mount.

About this instructionmanual This instructionmanualapplies to the DP23 and DP28 microscope digital cameras. Informationabout the operationof the microscope, computer or software canbe found in the instructionmanuals for the corresponding device or software.

Trademark Microsoft and Windows are registered trademarks of Microsoft Corporation, USA. All other brand names and product names mentioned in this manual are registered trademarks or trademarks of their respective owners.

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1 Safety The camera has beendeveloped and produced inconformitywith recognized safety regulations. However, it is not possible to entirely rule out possible hazards for the user, damage to the camera or microscope, or impairments of the camera's functionality. For this reason, always read and follow the general safety instructions and warnings before and during installation.

1.1 Intended use This camera is intended to be used for the acquisitionof digital images, but not for medicaldiagnostic purposes.

1.2 Avoiding personal injury and device damage

WARNING - Avoiding personal injury Electric shock due to damaged cable Whena cable is frayed or damaged, a person touching it risks receiving anelectric shock. Shut off the hardware and replace the cable immediately. Poisoning due to toxic gases In the event of a fire, the materialof the camera cangive off toxic gases. When these are inhaled, it cancause irritationor damage to the respiratory tract. Wear respiratoryprotectionwhen fighting the fire.

DP23 /DP28

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CAUTION - Avoiding personal injury Tripping hazard Cables laid across a room canbe a dangerous tripping hazard. This cancause injuries to people and damage to equipment. Wherever possible, lay cables along the wall or behind furniture. Fix and label cables that runacross the room.

ATTENTION - Avoiding device damage Never open the camera The camera's CMOS chip is extremely sensitive. Electrostatic discharge canpermanentlydamage the camera. Therefore, never open the camera housing. Use only the supplied USB camera cable Operate the camera onlywith the supplied USB camera cable. Only thenwill the functionalityof the camera and compliance with the EMC basic standards be ensured. Don't touch the protective glass The protective glass over the CMOS chip is extremely sensitive and must not come into contact withbare hands or anyother objects, otherwise the acquired images will be impaired by fingerprints or scratches. Avoid vibration shocks Mechanical shocks and strong vibrations candamage the camera. Don't drop the camera during assembly. Make sure the camera is securelymounted on the microscope. When the camera is not inuse, store it ina safe place.

1 Safety

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Observe the operating and storage conditions Exposure to high temperatures or humidity candamage component parts of the camera. Always observe the operating and storage conditions (see page 12).

1.3 Explanation of the symbols used

WARNING Indicates a potentially hazardous situation which, if not avoided, may result in major injury.

CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury.

ATTENTION Indicates a situation which, if not avoided, may damage the equipment or other property.

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2 The DP23 / DP28 camera 2.1 Scope of supply

Camera

USB camera cable

Instructionmanual

2 The DP23 /DP28 camera

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2.2 System diagram To use the camera witha light microscope, youneed suitable software and a computer as well as a monitor. For the recommended computer and monitor configuration, refer to page 14.

Youcanuse the following Olympus software applications:

Software application Minimum version

cellSens Life science image analysis software 3.1.1. OLYMPUS Stream Materials solutions image analysis software 2.4.4.

DP2-TWAIN Free image acquisition software 9.1 DP2-TWAIN is available only as a download from the Olympus Internet site.

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DP23/DP28/withDP2-AOU Alternatively, Olympus provides a special controlunit, DP2-AOU, for DP23 /DP28. DP2-AOU replaces a computer. The software for controlling DP23 /DP28 is pre-installed onDP2-AOU.

2.3 LED status indication A blue LED is located on the front of the camera. After starting the software, the LED indicates the following statuses: LED Status The LED flashes. The camera is being initialized.

The LED flashes continuously. There is a camera error. Contact Customer Service (see page 26).

The LED lights up continuously. The camera is ready.

The LED doesnt light up.

The camera is not connected to the computer or DP2-AOU port. The camera is not connected to the USB 3.1 port.

2 The DP23 /DP28 camera

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2.4 Specifications The DP23 /DP28 cameras are CMOS color cameras witha high frame rate. Theywere speciallydeveloped to acquire images from light microscopes. The cameras canbe connected to all commontypes of light microscopes bya C-mount adapter.

Specifications - DP23 Chip type C-mount CMOS camera Chip size 1/1.8 Inch

Recording area 7.41 mm x 4.98 mm 8.92 mm diagonal

Maximum resolution 3088 x 2076 pixels 6.41 million pixels

Camera port of the microscope C-Mount

Input / output

On the camera: USB 3.1 type C (with lock screws) On the computer or DP2-AOU : USB 3.1 type A

Resolution Frame rate* Exposure time

3088 x 2076 Full resolution 45 fps

13 s - 15 s 2072 x 2072 Square 59 fps 1544 x 1038 High speed 59 fps 1544 x 1038 High sensitivity 59 fps 1920 x 1080 Full HD 60 fps * The maximum achievable frame rate is listed. The frame rate depends, among other things, on the performance of the computer being used.

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Specifications - DP28 Chip type C-mount CMOS camera Chip size 1 Inch

Recording area 14.158 mm x 7.5 mm 16.02 mm diagonal

Maximum resolution 4104 x 2174 pixels 8.92 million pixels

Camera port of the microscope C-Mount

Input / output

On the camera: USB 3.1 type C (with lock screws) On the computer or DP2-AOU : USB 3.1 type A

Resolution Frame rate* Exposure time

4104 x 2174 Full resolution 32 fps

27 s - 15 s

2168 x 2168 Square 33 fps 2052 x 1086 High speed 64 fps 2052 x 1086 High sensitivity 32 fps 3840 x 2160 4K 33 fps 1920 x 1080 Full HD 64 fps * The maximum achievable frame rate is listed. The frame rate depends, among other things, on the performance of the computer being used.

The frame rate corresponds to the maximum display speed of the live-image (frame rate in frames/second (fps)).

2 The DP23 /DP28 camera

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2.5 Operating and storage conditions Always meet the operating and storage conditions.

Operating environment Ambient temperature +5 C - +40C Relative humidity 5% - 85% (without condensation) Altitude Up to 2000 m

Storage conditions Ambient temperature -20C - +60C Relative humidity 10% - 90%

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3 System environment 3.1 Microscope and camera adapter

Connectable Microscope Upright microscopes BX43/ BX53/ BX46/ BX63 CX23/ CX33/ CX43 MX63 / BX53M Inverted microscopes IX73/ IX83 GX53/ GKX53 Macro zoom microscope MVX10 Stereo microscopes SZX7/ SZX10/ SZX16/ SZ61TR

Connectable Camera Adapter DP23 DP28

U-TV1XC / U-TV1X+U-CMAD3

O O

U-TV0.63XC O (-)* U-TV0.5XC-3 O - U-TV0.35XC-2 (-)* - MVX-TV1XC O O MVX-TV0.63XC O - GX-TV0.7XC O - GX-TV0.5XC O - GX-TV0.5XC-DP O - 0.5XC (integrated in SZ61TR) O -

O Compatible

( - ) Limited compatible. This camera adapter may lead to shading effects towards the edges of the image.

- Not compatible as optical performance would be affected.

* Only use in combination with objectives/microscopes that support FN26.5. Otherwise shading effects towards the edges of the image may occur.

3 System environment

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3.2 Recommended computer and monitor configuration Whenyouconnect the DP23 /DP28 to a computer, the following system requirements must be met.

CPU Intel Core i5, Intel Core i7, Intel Core i9 Intel Xeon (or equivalent)

RAM Recommendation: 8 GB or more Monitor Resolution 1920 x 1080 or higher Communication port USB-3.1 type A Removable media drive Optical drive (double-layer capable)

Computer input device Recommendation: 3-button mouse with wheel. Keyboard

Operating system Microsoft Windows 10 (64 Bit)

3.3 Restrictions on use DP23 /DP28 withother microscopes DP23 /DP28 canbe used incombinationwithmanyavailable microscopes. However, the performance of the camera canonlybe guaranteed for the microscopes listed in the "Microscope and camera adapter" chapter onpage 13. The performance of the DP23 /DP28 whenused witha non-Olympus microscope has not beenevaluated.

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Brightness fluctuations in the camera image Withveryhigh illumination intensities and short exposure times, the following phenomena canoccur:

n Flickering in the image displayed n Exposure fluctuation

If possible, reduce the illumination light levelor use anattenuation filter to increase the exposure time to 20 ms or longer.

Obscured peripheryof the field of view due to long intermediate tubelength If the intermediate tubelength is long (see examples below), the peripheryof the field of view maybe obscured depending on the observation tube and objective used together. Examples when the intermediate tubelength is long:

n 2 or more intermediate tubes are used ina stack. n BX3 series: The reflected light illuminator and the

intermediate adapter are combined. n IX3 series: IX73P2F or IX83P2ZF is used as a microscope

frame. n All reflected microscopes whena brightfield or polarization

observationmethod is used. Exceptions are the BX53MRF-S and BX53MTRF-S microscope frames when the reflected light illuminator BX3M-KMA-S is used.

3 System environment

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Spot flare witha small diameter of the aperture diaphragm (AS) A spot flare maybe noticeable, if the aperture diaphragm (AS) is almost completely closed. This flare becomes less noticeable if you open the aperture diaphragm (AS).

No support of simultaneous image acquisitionusing several cameras Youcanconnect severalDP23 /DP28 cameras to one microscope. However, it isnt possible to simultaneouslyoperate several cameras withone computer.

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4 Assembly 4.1 Attaching the camera

1. Remove the cap of the C-mount port (a). 2. Screw the C-mount camera adapter (b) into the C-mount

thread (c) at the bottom of the camera head. 3. Attach the C-mount camera adapter to the camera port of

the microscope.

Checking the cameras orientation If the camera is not attached in the correct orientation, the camera image does not match the image visible through the eyepieces.

1. After attaching the camera, compare the eyepiece image with the live image on the monitor.

2. Rotate the camera adapter so that the alignment of these images matches.

4 Assembly

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4.2 Connecting the USB camera cable

ATTENTION

Use only the supplied USB camera cable Operate the camera only with the supplied USB camera cable. Only then will the functionality of the camera and compliance with the EMC basic standards be ensured.

1. Connect the USB type Cplug (a) to the cameras USB type C socket (b) and secure it with the two screws (c).

2. Connect the USB camera cable's USB type A plug (d) to the USB 3.1 port (e)of the computer or the DP2-AOU. Depending on the computer, the USB 3.1 port is either colored blue or labeled "SS". The camera cant be operated using a USB 2.0 port.

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4.3 Installing the software cellSens/OLYMPUS Stream software Read the cellSens/OLYMPUS Stream installationmanualbefore installing the software. Whenyoupurchase DP2-AOU, the required software is pre-installed.

TWAIN Driver DP2-TWAIN DP2-TWAIN is available onlyas a download from the Olympus Internet site.

4 Assembly

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5 Acquiring images Youcan find detailed instructions in the instructionmanualor in the Help of the software youare using.

Example of the acquisitionprocess for brightfield images

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6 Cleaning the camera Whencorrectlyhandled, the camera has a long life spanand is to a large extent maintenance-free.

Cleaning the protective glass To clean the camera's protective glass, use a standard rubber air blower to remove the dust and clean it gentlywitha clean lens cloth. If there are fingerprints or oil smudges on the protective glass, carefully clean it witha clean lens clothmoistened withpure alcohol.

Cleaning the housing Parts other than the glass components should be cleaned witha clean cloth. Do not use organic solvents to remove major stains. Use a soft clothmoistened witha neutraldetergent solution.

ATTENTION

Never open the camera The camera's CMOS chip is extremely sensitive. Electrostatic discharge can permanently damage the camera. Therefore, never open the camera housing.

6 Cleaning the camera

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7 Troubleshooting 7.1 Possible problems

Problem: The camera is not working Possible cause Remedy

The USB camera cable is not connected properly.

Connect the USB camera cable properly. When the USB camera cable is connected properly, the LED lights up continuously (see page 17).

Problem: Live image is not displayed Possible cause Remedy The illumination of the microscope is not turned on. Switch on the microscope illumination.

The light path selector of the microscope is not set to the camera.

Set the light path selector of the microscope to the camera output.

The illumination intensity of the microscope is not adjusted properly.

Set the illumination intensity of the microscope correctly.

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Problem: A snapshot cannot be acquired Possible cause Remedy DP2-TWAIN, cellSens or OLYMPUS Stream is still processing or performing data storage after acquisition.

Wait until the processing is finished. Then acquire the next snapshot.

The computer's RAM is fully occupied.

Close other software applications. Then acquire the next snapshot.

There are too many images loaded in your software.

If necessary, save the images. Close the loaded images. Then acquire the next snapshot.

Problem: The image is too bright Possible cause Remedy The exposure compensation is set too high to the + side.

Set the exposure compensation to a more suitable value.

A dark area of the image was mistakenly set as the metering area

Set a more suitable metering area.

The AE lock is set at an exposure time that is too long. Deactivate the AE lock.

The illumination of the microscope is too bright.

Reduce the illumination light level or use an attenuation filter.

The manual exposure time is too long. Reduce the manual exposure time.

7 Troubleshooting

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Problem: The image is too dark Possible cause Remedy Exposure compensation is set too low to the - side.

Set the exposure compensation to a more suitable value.

A bright area of the image was mistakenly set as the metering area

Set a more suitable metering area.

The AE lock is set at an exposure time that is too short. Deactivate the AE lock.

The illumination of the microscope is too dark.

Increase the illumination light level or remove attenuation filters.

The manual exposure time is set too low. Increase the manual exposure time.

Problem: The colors of the image are inaccurate Possible cause Remedy The reference area for white balance was not selected appropriately.

Set a reference area for white balance in which nothing can been seen in the background.

The screen color of the computer is not set correctly.

Set the screen color of the computer to 24 bit or higher. 32 bit is recommended.

The color space was not selected appropriately.

Change the camera settings in your software. Select the appropriate color adjustment which is suitable for your microscope, observation method and samples.

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Problem: The acquired image is not in focus Possible cause Remedy The microscope was not focused on the sample. Focus the microscope on the sample.

The parfocality between the camera adapter and the eyepieces is not adjusted properly.

Adjust the parfocality between the camera adapter and the eyepieces properly.

The aperture diaphragm of the condenser is too wide open.

Set the aperture diaphragm to 75% of the objectives numerical aperture and focus the microscope on the sample.

The field diaphragm is too wide open.

Narrow the field aperture appropriately and focus the microscope on the sample.

Lens components of the microscope and the cover glass on the bottom of the camera are dirty.

Clean the objective, condenser, window lens of the microscope, and the cover glass on the bottom of the camera (see page 21).

The microscope and camera are being subjected to vibration during acquisition.

Acquire images in an environment where the microscope and camera are not subjected to vibration. It is effective to use an anti-vibration table.

7 Troubleshooting

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7.2 Contacting Customer Service Please contact your localOlympus support if youhave anyquestions about the product. The addresses canbe found on the following websites:

Life Science Solutions https://www.olympus-lifescience.com/en/contact-us/

Industrial Solutions https://www.olympus-ims.com/en/contact-us/

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8 Notes on conformity and disposal CE Conformity (Europe) This product complies with the requirements of Directive 2014/30/EU concerning electromagnetic compatibility according to Standard IEC/EN61326-1.

n Emission: Class B n Immunity: Applied to industrial and basic requirements.

WEEE declaration (Europe) Inaccordance with the Europeandirective onWaste of Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste but should be collected separately. Refer to your local authority in the EU for returnand/or collection systems available in your country.

RoHS conformity (Europe) This Olympus Soft Imaging Solutions camera conforms with the EuropeanUniondirective on the restrictionof the use of certain hazardous substances inelectrical and electronic equipment 2011/65/EU.

8 Notes onconformityand disposal

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FCCconformity (USA) This device complies withpart 15 of the FCCRules. Operation is subject to the following conditions: (1) this device maynot cause harmful interference, and (2) this device must accept any interference received, including interference that maycause undesired operation. This device has been tested and found to complywith the limits for a Class B digitaldevice, pursuant to Part 15 of the FCCRules. These limits are designed to provide reasonable protectionagainst harmful interference ina residential installation. This device generates, uses, and can radiate radio frequencyenergy. If not installed and used inaccordance with the instructions, it maycause harmful interference to radio communications. However, there is no guarantee that interference will not occur ina particular installation. If this equipment does cause harmful interference to radio or television reception, whichcanbe determined by turning the equipment off and on, the user is encouraged to try to correct the interference byone or more of the following measures:

1. Reorient or relocate the receiving antenna 2. Increase the distance between the equipment and receiver 3. Connect the equipment into anoutlet ona circuit different

from that to which the receiver is connected 4. Consult the dealer or anexperienced radio/TV technician for

help

FCCwarning Changes or modifications not expressly approved by the party responsible for compliance could void users authority to operate the equipment.

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FCC Supplier's Declaration of Conformity The supplier hereby declares that the product

Product name:   Optical Microscope Accessory Model Number:   DP23-CU

Conforms to the following specifications: FCC Part 15, Subpart B, Section 15.107 and Section 15.109

Supplementary Information: This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

Responsible Party Name: Olympus Scientific Solutions Americas Corp. Address:              48 Woerd Ave Waltham, MA 02453, U.S.A. Phone Number:   781-419-3900

FCC Supplier's Declaration of Conformity The supplier hereby declares that the product

Product name:   Optical Microscope Accessory Model Number:   DP28-CU

Conforms to the following specifications: FCC Part 15, Subpart B, Section 15.107 and Section 15.109

Supplementary Information: This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

Responsible Party Name: Olympus Scientific Solutions Americas Corp. Address:              48 Woerd Ave Waltham, MA 02453, U.S.A. Phone Number:   781-419-3900

8 Notes onconformityand disposal

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Korea . Trade Name or Registrant: Equipment Name:

Olympus Soft Imaging Solutions GmbH OpticalMicroscope Accessory

Basic ModelNumber: RegistrationNo.:

DP23-CU R-R-OIS-68000000

Basic ModelNumber: RegistrationNo.:

DP28-CU R-R-OIS-69000000

Manufacturer/ Countryof Origin:

Olympus Soft Imaging Solutions GmbH, Germany

China RoHS conformity (China)

( )

(Pb)

(Hg)

(Cd)

(Cr(VI))

(PBB)

(PBDE)

X O O O O O X O O O O O X O X O O O

O O O O O O SJ/T 11364

GB/T26572

XGB/T26572

DP23 /DP28

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www.olympus-global.com

Manufactured by OLYMPUS SOFT IMAGING SOLUTIONS GmbH

Johann-Krane-Weg 39, 48149 Mnster, Germany

Distributed by OLYMPUS CORPORATION

Shinjuku Monolith, 2-3-1, Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

OLYMPUS EUROPA SE & CO. KG Wendenstrasse 20, 20097 Hamburg, Germany

OLYMPUS CORPORATION OF THE AMERICAS 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A.

OLYMPUS CORPORATION OF ASIA PACIFIC LIMITED L26, Tower 1, Kowloon Commerce Centre, No. 51 Kwai Cheong Road,

Kwai Chung, New Territories, Hong Kong

OLYMPUS AUSTRALIA PTY LTD 3 Acacia Place, Notting Hill, 3168, Austra

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