Agilent InfinityLab LC Series Thermostat User Manual PDF

Agilent InfinityLab LC Series Multicolumn Thermostats

User Manual

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual

Notices

Document Information Document No: SD-29000232 Rev. C Edition: 02/2022

Copyright Agilent Technologies, Inc. 2017-2022

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Safety Notices

CAUTION A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARN- ING notice until the indicated conditions are fully understood and met.

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Agilent InfinityLab LC Series Multicolumn Thermostats User Manual

In This Book

This manual covers the following modules:

Agilent 1290 Infinity II Multicolumn Thermostat (G7116B)

Agilent 1260 Infinity II Multicolumn Thermostat (G7116A)

1 Introduction to the Multicolumn Thermostat

This chapter gives an introduction to the MCT and an instrument overview.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical and performance specifications.

3 Using the Module

This chapter gives instructions on how to use the module.

4 Preparing the Module

This chapter provides information on how to set up the module for an analysis and explains the basic settings.

5 Optimization

This chapter provides information on how to optimize the Multicolumn Thermostat.

6 Troubleshooting and Diagnostics

Overview about the troubleshooting and diagnostic features.

7 Error Information

This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 3

8 Test Functions and Calibrations

This chapter describes the module's built-in test functions.

9 Maintenance

This chapter describes the maintenance of the MCT.

10 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

11 Identifying Cables

This chapter provides information on cables used with the Agilent Infinity and Infinity II Series modules.

12 Hardware Information

This chapter describes the module in more detail on hardware and electronics.

13 Appendix

This chapter provides addition information on safety, legal and web.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 4

Contents

1 Introduction to the Multicolumn Thermostat 8

Product Description (G7116B) 9 Product Description (G7116A) 10 Features (G7116B) 11 Features (G7116A) 12 Operating Principle 13 Column Switching Valve 14 Typical Applications 17 Leak and Waste Handling 22

2 Site Requirements and Specifications 27

Site Requirements 28 Physical Specifications 31 Performance Specifications 33 Valve Specifications 37 Extended Specifications 40

3 Using the Module 41

Magnets 42 Turn on/off 43 Status Indicators 45 Open the Front Door 46 Install Heat Exchanger 47 Exchange a Column 51 Installing Valve Heads 54 Installing the capillaries 60 Install the Divider Assembly 62 Install the Thermal Equilibration Device 63 Installing and Using Column ID Tags 65 Agilent Local Control Modules 81

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 5

4 Preparing the Module 82

Leak and Waste Handling 83 Solvent Information 85 Capillary Coding Guide 92 Install Capillary Connections 95

5 Optimization 100

Optimizing the Column Compartment 101

6 Troubleshooting and Diagnostics 102

Available Tests vs User Interfaces 103 Agilent Lab Advisor Software 104

7 Error Information 105

What Are Error Messages 106 General Error Messages 107 MCT Error Messages 113

8 Test Functions and Calibrations 119

Tests and Calibrations in Agilent Lab Advisor 120 Thermostat Test 121 System Pressure Test 122 Sensors Offset Calibration 123

9 Maintenance 124

Introduction to Maintenance 125 Cautions and Warnings 127 Overview of Maintenance 129 Cleaning the Module 130 Correcting Leaks 131 Maintain the Column Switching Valve 132 Replace Valve Heads 135 Prepare the MCT for Transportation 139 Replace the Module Firmware 140

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 6

10 Parts and Materials for Maintenance 141

Plastic Parts 142 Leak Parts 143 Valve Options and Parts 144 Column ID Parts 150 Accessories and Consumables 151

11 Identifying Cables 163

Cable Overview 164 Analog Cables 166 Remote Cables 168 CAN/LAN Cables 172 RS-232 Cables 173 USB 174

12 Hardware Information 175

Firmware Description 176 Electrical Connections 179 Interfaces 182 2-bit Configuration Switch 190 Instrument Layout 191 Early Maintenance Feedback (EMF) 192

13 Appendix 193

General Safety Information 194 Waste Electrical and Electronic Equipment (WEEE) Directive 200 Radio Interference 201 Sound Emission Certification for Federal Republic of Germany 202 Agilent Technologies on Internet 203

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 7

1 Introduction to the Multicolumn Thermostat

Product Description (G7116B) 9

Product Description (G7116A) 10

Features (G7116B) 11

Features (G7116A) 12

Operating Principle 13

Column Switching Valve 14 Multicolumn Selection (G7116B with G4239C) 14 Multicolumn Selection (G7116A with G4237A) 15 Method Development 16

Typical Applications 17 Dual column selection (2-position/6-port or 2-position/10-port valves) 18 Sample enrichment and sample cleanup (2-position/6-port or 2-position/10-port valves) 19 Alternating Column Regeneration (2-position/10-port valves only) 21

Leak and Waste Handling 22 Waste Concept 26 Leak Sensor 26

This chapter gives an introduction to the MCT and an instrument overview.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 8

1 Introduction to the Multicolumn Thermostat Product Description (G7116B)

Product Description (G7116B)

The Agilent 1290 Infinity II Multicolumn Thermostat (MCT) facilitates precise column thermostatting over a broad temperature range with cooling down to 20 C below ambient temperature and heating up to 110 C.

This capability provides high flexibility for optimized speed and enhanced selectivity of LC separations. Exchangeable ultra-high-pressure valves enable a wide range of applications such as column selection from eight columns in a single MCT, sample preparation for analyte enrichment or matrix removal, alternating column regeneration and many more.

The MCT matches perfectly with all InfinityLab LC Series systems and can also be combined with previous 1260 and 1290 Infinity Series modules.

Figure 1 Overview of the Multicolumn Thermostat

Power switch

Status indicator

Valve

Leak drain

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 9

1 Introduction to the Multicolumn Thermostat Product Description (G7116A)

Product Description (G7116A)

The Agilent 1260 Infinity II Multicolumn Thermostat (MCT) facilitates precise column thermostatting over a broad temperature range with cooling down to 10 C below ambient temperature and heating up to 85 C.

This capability provides robust and reliable separations for maximum application flexibility. Exchangeable high-pressure valves enable a wide range of applications such as column selection of up to four columns, sample preparation for analyte enrichment or matrix removal, or alternating column regeneration.

The MCT matches perfectly with all InfinityLab LC Series systems and can also be combined with 1290 Infinity II Series modules as well as with previous 1260 Infinity and 1290 Infinity Series modules.

Figure 2 Overview of the Multicolumn Thermostat

Status indicator

Valve

Power switch

Leak drain

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 10

1 Introduction to the Multicolumn Thermostat Features (G7116B)

Features (G7116B)

Superior usability with flexible flap positions: open door to 90 (desk function), or to 180, or even removal of the front door for maximum accessibility.

Efficient, fast, and most convenient column exchange through InfinityLab Quick Connect Fittings.

Advanced column capacity for up to 8 columns in a single MCT.

Next generation InfinityLab Quick Connect Heat Exchangers for precolumn solvent thermostatting, which contribute volumes of only 3, 1.6 or 1 L for optimized low dispersion.

Maximum application flexibility through Peltier cooling and heating with two independent temperature zones from 20 C below ambient temperature up to 110 C.

Optional valve drive to host user-exchangeable InfinityLab Quick Change Valves of different formats.

High temperature precision for reproducible retention times and peak areas.

Optional column identification module to track history of up to eight columns for documentation of column type, major column parameters, and number of injections as standard for GLP.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 11

1 Introduction to the Multicolumn Thermostat Features (G7116A)

Features (G7116A)

Superior usability with flexible flap positions: open door to 90 (desk function), or to 180, or even removal of the front door for maximum accessibility.

Efficient, fast, and most convenient column exchange through InfinityLab Quick Connect Fittings.

Reproducible precolumn solvent thermostatting with next generation InfinityLab Quick Connect Heat Exchanger easily installed for each column. A bio-inert heat exchanger is available for biological and extreme pH applications.

Maximum application flexibility through Peltier cooling and heating with two independent temperature zones from 10 C below ambient temperature up to 85 C.

Optional, traceable column identification to track history of up to four columns for documentation of column type, major column parameters, and number of injections as standard for GLP.

Optional valve drive to host user-exchangeable InfinityLab Quick Change Valve Heads of different formats, which are also available in bio-inert materials.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 12

1 Introduction to the Multicolumn Thermostat Operating Principle

Operating Principle

Figure 3 Hydraulic path

The Concept of Heating and Cooling

The design of the Multicolumn Thermostat (MCT) uses Quick Connect Heat Exchangers that are mounted on to the heating and cooling devices with Peltier elements. The solvent entering the Quick Connect Heat Exchangers is heated up or cooled down to a settable temperature before entering the column. A triangular shape of the Quick Connect Heat Exchangers prevents incorrect placement and ensures optimum heat exchange of the solvent. Optimal insulation of the heater room offers best temperature stability for the column. This ensures that the column and the solvent flowing through it are almost at the same temperature. The solvent cools down or heats up on its transfer from the Quick Connect Heat Exchangers to the column inlet. This depends on several factors: flow rate, setpoint temperature, and ambient temperature. Any type of heated column compartment brings one important consequence for column temperature equilibration. Before an equilibrium is reached, the whole mass of column, column packing, and solvent volume inside the column has to be brought to the selected temperature. This depends on several factors: flow rate, setpoint temperature, ambient temperature, and column dimensions. The column equilibrates faster at higher flow rates (due to thermostatted mobile phase). In a flowthrough temperature regulation system, there are necessarily slightly different temperatures at different positions. The actual temperature displayed on the user interface is always the temperature measured at the heating and cooling device.

The equilibration of the column may take a while. Stability of the pressure signal is a good indication for temperature equilibrium of the column.

Multisampler

Detector

Column

Heat exchanger

Valve

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 13

1 Introduction to the Multicolumn Thermostat Column Switching Valve

Column Switching Valve

InfinityLab Quick Change Valves for column switching are an orderable add-on option for the InfinityLab Multicolumn Thermostat.

Multicolumn Selection (G7116B with G4239C)

Advantages

Increase productivity

Higher instrument up-time

The valve facilitating quick changes allows the selection between up to eight different stationary phases for a variety of applications, or the usage of identical stationary phases in columns with different dimensions for either faster run-times (short columns) or higher resolution (long columns) or for loading studies with different internal diameters.

Figure 4 The G7116B 1290 Infintiy II Multicolumn Thermostat equipped with an InfinityLab Quick Change 8-column selector valve head

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 14

1 Introduction to the Multicolumn Thermostat Column Switching Valve

Multicolumn Selection (G7116A with G4237A)

Advantages

Increase productivity

Higher instrument up-time

The valve facilitating quick changes allows the selection between up to four different stationary phases for a variety of applications, or the usage of identical stationary phases in columns with different dimensions for either faster run-times (short columns) or higher resolution (long columns) or for loading studies with different internal diameters.

Figure 5 The G7116A 1260 Infinity II Multicolumn Thermostat equipped with an InfinityLab Quick Change 4-column selector valve head

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 15

1 Introduction to the Multicolumn Thermostat Column Switching Valve

Method Development

Advantages:

Faster method development

Automated method development possible

Figure 6 Different chromatographic results obtained for the same sample on five different stationary phases

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 16

1 Introduction to the Multicolumn Thermostat Typical Applications

Typical Applications

Refer to the table below for further information on which ports are connected to which position.

NOTE The interconnection of ports at particular valve position strongly depends on the combination of valve and module. The software user interface always displays the correct situation. A method modification or re-plumbing of the connections is typically required if transferring methods from G1316A/B/C to G7116A/B, G1170A or G4227A.

Modules Valve Position 1 Position 2

G1316A/B/C 2-position/6-port 1-2 1-6

G7116A/B, G1170A, G4227A 2-position/6-port 1-6 1-2

G1316A/B/C 2-position/10-port 1-2 1-10

G7116A/B, G1170A, G4227A 2-position/10-port 1-10 1-2

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 17

1 Introduction to the Multicolumn Thermostat Typical Applications

Dual column selection (2-position/6-port or 2-position/10-port valves) Advantages:

Increase productivity

Higher instrument up-time

The valve can select either column 1 or column 2, allowing quick changes between two different stationary phases for separation selectivity, or immediate availability of a second and identical stationary phase in case the first column loses efficiency, when dealing with complex matrices for instance.

To detector

From pump Position 1-2

Column 1

Column 2 To detector

From pump Position 1-6

Column 1

Column 2

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 18

1 Introduction to the Multicolumn Thermostat Typical Applications

Sample enrichment and sample cleanup (2-position/6-port or 2-position/10-port valves)

Sample Enrichment

Sample Cleanup

Position 1-6

Pump A / autosampler

Waste

Enrichment column

Pump B

Analytical

detector column /

Position 1-2

Pump A / autosampler

Waste

Enrichment column

Pump B

Analytical

detector column /

Pump A / autosampler

Detector Analytical column Waste

Pump B

Precolumn

Position 1-6 Position 1-2

Pump A / autosampler

Detector Analytical column Waste

Pump B

Precolumn

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 19

1 Introduction to the Multicolumn Thermostat Typical Applications

Advantages:

Easy automation of sample preparation

Higher reproducibility

Increased productivity and sensitivity

Sample cleanup is essential for samples with complex matrices, such as biological fluids, food extracts and waste water. Before injection into a LC or LC/MS system, the sample matrix must be separated from the analytes of interest. Otherwise, contaminants can disrupt separation and detection or even damage the analytical column.

Enrichment methods

Enrichment methods are the techniques of choice to obtain highest sensitivity and to remove the sample matrix in such applications as proteomics, drug metabolism, and environmental trace analysis. The analytes are retained and concentrated onto the precolumn, while the sample matrix is passed to waste. After the valve switch, a second pump backflushes the analytes out of the precolumn onto the separation column. This allows injection of large volumes onto the precolumn, significantly expanding sensitivity in the range of ten to several thousands.

Sample cleanup

Cleanup methods handle analytes and matrices in the opposite way to enrichment methods. Matrix components are retained on the precolumn while the analytes pass through to the separation column. After the valve switches, an extra pump backflushes the matrix components out of the precolumn to waste, while the analytes are separated on the main column. Backflushing prepares the precolumn for the next injection.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 20

1 Introduction to the Multicolumn Thermostat Typical Applications

Alternating Column Regeneration (2-position/10-port valves only) Advantages:

High sample throughput

Increased productivity

High efficiency

Gradient elution is frequently used for fast separation of complex samples in LC. Since the gradient elution requires the column to regenerate before subsequent runs, an automated column regeneration system saves valuable analysis time. Agilent's InfinityLab Quick Change 2-position/10-port valve enables the simultaneous analysis of one sample on one LC column while an extra regeneration pump flushes and equilibrates a second, identical column. At the end of the run, the valve switches to the second position and the next sample is separated on the previously flushed and equilibrated column. Meanwhile, the regeneration pump flushes and equilibrates the first column. Up to 50 % of analysis time is often required to equilibrate columns. Using alternating column regeneration saves time and provides higher sample throughput.

Column 2

Column 1

Eluent pump / Autosampler

Regeneration pump

Waste

Detector

Position 1-10

Column 2

Column 1

Eluent pump / Autosampler

Regeneration pump

Waste

Detector

Position 1-2

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 21

1 Introduction to the Multicolumn Thermostat Leak and Waste Handling

Leak and Waste Handling

The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.

The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).

All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other. Waste tubes are guided through a channel on the right hand side of the instrument, keeping the front access clear from tubes.

The leak plane provides leak management by catching all internal liquid leaks, guiding them to the leak sensor for leak detection, and passing them on to the next module below, if the leak sensor fails. The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached.

Solvent and condensate is guided through the waste channel into the waste container:

from the detector's flow cell outlet

from the Multisampler needle wash port

from the Sample Cooler or Sample Thermostat (condensate)

from the pump's Seal Wash Sensor (if applicable)

from the pump's Purge Valve or Multipurpose Valve

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 22

1 Introduction to the Multicolumn Thermostat Leak and Waste Handling

Figure 7 Infinity II Leak Waste Concept (Flex Bench installation)

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 23

1 Introduction to the Multicolumn Thermostat Leak and Waste Handling

Figure 8 Infinity II Single Stack Leak Waste Concept (bench installation)

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 24

1 Introduction to the Multicolumn Thermostat Leak and Waste Handling

Figure 9 Infinity II Two Stack Leak Waste Concept (bench installation)

The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 25

1 Introduction to the Multicolumn Thermostat Leak and Waste Handling

Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with

4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid.

Leak Sensor

CAUTION Solvent incompatibility

The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor. The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.

Do not use DMF as mobile phase.

Check the leak sensor regularly for corrosion.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 26

2 Site Requirements and Specifications

Site Requirements 28

Physical Specifications 31 Physical Specifications G7116B 31 Physical Specifications G7116A 32

Performance Specifications 33 Performance Specifications G7116B 33 Performance Specifications G7116A 35

Valve Specifications 37

Extended Specifications 40

This chapter provides information on environmental requirements, physical and performance specifications.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 27

2 Site Requirements and Specifications Site Requirements

Site Requirements

A suitable environment is important to ensure optimal performance of the instrument.

Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 31. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.

WARNING Hazard of electrical shock or damage of your instrumentation

can result, if the devices are connected to a line voltage higher than specified.

Connect your instrument to the specified line voltage only.

WARNING Electrical shock hazard

The module is partially energized when switched off, as long as the power cord is plugged in.

The cover protects users from personal injuries, for example electrical shock.

Do not open the cover.

Do not operate the instrument and disconnect the power cable in case the cover has any signs of damage.

Contact Agilent for support and request an instrument repair service.

WARNING Inaccessible power plug.

In case of emergency it must be possible to disconnect the instrument from the power line at any time.

Make sure the power connector of the instrument can be easily reached and unplugged.

Provide sufficient space behind the power socket of the instrument to unplug the cable.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 28

2 Site Requirements and Specifications Site Requirements

Power Cords Country-specific power cords are available for the module. The female end of all power cords is identical. It plugs into the power-input socket at the rear. The male end of each power cord is different and designed to match the wall socket of a particular country or region.

Agilent makes sure that your instrument is shipped with the power cord that is suitable for your particular country or region.

WARNING Unintended use of power cords

Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment.

Never use a power cord other than the one that Agilent shipped with this instrument.

Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

WARNING Absence of ground connection

The absence of ground connection can lead to electric shock or short circuit.

Never operate your instrumentation from a power outlet that has no ground connection.

WARNING Electrical shock hazard

Solvents may damage electrical cables.

Prevent electrical cables from getting in contact with solvents.

Exchange electrical cables after contact with solvents.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 29

2 Site Requirements and Specifications Site Requirements

Bench Space The module dimensions and weight (see Table 1 on page 31) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.

If the bench shall carry a complete HPLC system, make sure that the bench is designed to bear the weight of all modules.

The module should be operated in a horizontal position.

Condensation

NOTE Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench rack. This option helps to save bench space as all modules can be placed into one single stack. It also allows to easily relocate the instrument to another lab.

CAUTION Condensation within the module

Condensation can damage the system electronics.

Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module.

If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 30

2 Site Requirements and Specifications Physical Specifications

Physical Specifications

Physical Specifications G7116B

Table 1 Physical Specifications G7116B

Type Specification Comments

Weight 12.5 kg (27.6 lbs)

Dimensions (height width depth)

160 x 435 x 436 mm (6.3 x 17.1 x 17.2 inches) Width with column identification kit: 472 mm

G7116B may have a Column ID tag reader on both sides.

Line voltage 100 240 V~, 10 % Wide-ranging capability

Line frequency 50 or 60 Hz, 5 %

Power consumption 150 VA, 150 W

Ambient operating temperature

455 C (39131 F)

Ambient non-operating temperature

-40 70 C (-40 158 F)

Humidity < 95 % r.h. at 40 C (104 F) Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Safety standards: IEC, EN, CSA, UL

Overvoltage category II, Pollution degree 2 For indoor use only

ISM Classification ISM Group 1 Class B According to CISPR 11

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 31

2 Site Requirements and Specifications Physical Specifications

Physical Specifications G7116A

Table 2 Physical Specifications G7116A

Type Specification Comments

Weight 12.5 kg (27.6 lbs)

Dimensions (height width depth)

160 x 435 x 436 mm (6.3 x 17.1 x 17.2 inches) Width with column identification kit: 460 mm

G7116A only has a Column ID tag reader on the left hand side.

Line voltage 100 240 V~, 10 % Wide-ranging capability

Line frequency 50 or 60 Hz, 5 %

Power consumption 150 VA, 150 W

Ambient operating temperature

455 C (39131 F)

Ambient non-operating temperature

-40 70 C (-40 158 F)

Humidity < 95 % r.h. at 40 C (104 F) Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Safety standards: IEC, EN, CSA, UL

Overvoltage category II, Pollution degree 2 For indoor use only

ISM Classification ISM Group 1 Class B According to CISPR 11

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 32

2 Site Requirements and Specifications Performance Specifications

Performance Specifications

Performance Specifications G7116B

Table 3 Performance Specifications G7116B

Feature Specification Comment

Operating principle Thermostatted column compartment with dual, independent Peltier element. Solvent pre-heating and still-air operation for reduction of chromatographic band broadening under UHPLC-conditions. Up to four devices can be clustered and controlled by a single user

interface for additional flexibility.1

Temperature range 20 C below ambient (minimum 4 C) to 110 C 2

Temperature range increment

Settable in steps of 0.1 C 2

Temperature stability 0.03 C 2

Temperature accuracy

0.5 C (with calibration at 40 C) 2

Temperature precision

0.05 C 2

Independent temperature zones

2 (in single device)

Up to 8 in clustered configuration1

Column capacity 8 columns of 100 mm length plus Quick Connect fittings or pre-columns 4 columns of 300 mm length plus Quick Connect fittings or pre-columns Selection of columns by single optional integrated 8-column selector valve (1300 bar) Maximum of 32 columns of 100 mm length plus Quick Connect fittings or pre-columns 16 columns of 300 mm length plus Quick Connect fittings or pre-columns

with clustering1 of four devices.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 33

2 Site Requirements and Specifications Performance Specifications

Heat-up/cool-down time

5 min from ambient to 40 C 10 min from 40 C to 20 C <30 min from 25 C to 100 C

2

Solvent heat exchangers

Individually quick-installable for every column. Available at 1 L internal volume, 0.075 mm i.d. capillary (ultra-low dispersion), 1.6 L internal volume, 0.12 mm i.d. capillary (standard) and 3 L internal volume, 0.12 mm i.d. capillary (high-flow).

Valve options 1 x integrated valve drive as option 2 x external valve drives as option to host user-exchangeable Quick Change valve heads (up to 1300 bar) of different formats: 2-position/6-port, 2-position/10-port, 4-column selection (4-position/10-port) up to 800 bar, 6-column selection (6-position/14-port), 8-column selection (8-position/18-port). Valve heads are automatically identified by their RFID tag.

Column identification Optionally, column identification kit to track history of up to eight columns. Mounted on left- and right-hand side of module.

Instrument control LC and CE Drivers A.02.11 or above Agilent Instant Pilot (G4208A) B.02.19 or above InfinityLab LC Companion (G7108A) 1.1 or above. Lab Advisor B.02.06 or above

For details about supported software versions refer to the compatibility matrix of your version of the LC & CE Drivers.

Communication Controller Area Network (CAN) G7116B is a hosted module. (The LC stack needs to contain suitable host module or a LAN card for communication and control).

Maintenance and safety-related features

Extensive diagnostics, error detection and display (through Instant Pilot control module and Agilent Lab Advisor), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in main maintenance areas. Door-open sensor.

GLP features Valve heads carrying tags with serial number, pressure rating, number of switches and valve type. Concept of column identification.

Housing All materials recyclable

1 Requires LC and CE drivers A.02.12 or above

2 All specifications are valid for distilled water at ambient temperature (25 C), set point at 40 C and a stable flow range from 0.2 - 5 mL/min. Equilibration time: 10 min.

Table 3 Performance Specifications G7116B

Feature Specification Comment

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 34

2 Site Requirements and Specifications Performance Specifications

Performance Specifications G7116A

Table 4 Performance Specifications G7116A

Type Specification Comments

Operating principle Thermostatted column compartment with dual, independent Peltier-element. Solvent pre-heating and still-air operation for reduction of chromatographic band-broadening under UHPLC-conditions.

Temperature range 10 C below ambient (minimum 4 C) to 85 C 1

Temperature range increment

Settable in steps of 0.1 C 1

Temperature stability 0.1 C 1

Temperature accuracy

0.5 C (with calibration at 40 C) 1

Temperature precision

0.05 C 1

Independent temperature zones

2 in single device

Column capacity 4 columns of up to 300 mm length plus InfinityLab Quick Connect fittings or pre-column. The number of pre-column Quick Connect Heat Exchangers is scalable - each column can be equipped with an individual heat exchanger for best performance. 4-column selector valve is available to access each column without replumbing.

Heat-up/cool-down time

5 min from ambient to 40 C 10 min from 40 C to 20 C <25 min from 25 C to 85 C

1

Solvent heat exchangers

Individually quick-installable for every column. Available at 3 L internal volume, 0.17 mm i.d. capillary (large ID), 6 L internal volume, 0.17 mm i.d. capillary (large ID high flow) and 9 L internal volume, 0.17 mm i.d. capillary (bio-inert, metal-free).

Valve options 1 x integrated valve drive as option to host user-exchangeable Quick Change valve heads (up to 800 bar) of different formats: 2-position/6-port, 2-position/10-port, 4-column selection (4-position/10-port). Also available in bio-inert materials. Valve heads are automatically identified by their RFID tag.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 35

2 Site Requirements and Specifications Performance Specifications

Column identification Optionally, column identification kit to track history of up to four columns. Mounted on the left hand-side of module.

Instrument control LC and CE Drivers A.02.14 or above. Agilent Instant Pilot (G4208A) B.02.20 or above. InfinityLab LC Companion (G7108A) 1.1 or above. Lab Advisor B.02.08 or above.

For details about supported software versions refer to the compatibility matrix of your version of the LC & CE Drivers

Communication Controller Area Network (CAN) G7116A is a hosted module. (The LC stack needs to contain suitable host module or a LAN card for communication and control).

Maintenance and safety-related features

Extensive diagnostics, error detection and display (through Instant Pilot control module and Agilent Lab Advisor), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

GLP features Valve heads carrying tags with serial number, pressure rating, number of switches and valve type. Concept of column identification.

Housing All materials recyclable.

1 All specifications are valid for distilled water at ambient temperature (25 C), set point at 40 C and a stable flow range from 0.2 - 5 mL/min. Equilibration time: 10 min.

Table 4 Performance Specifications G7116A

Type Specification Comments

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 36

2 Site Requirements and Specifications Valve Specifications

Valve Specifications

Table 5 G4239C (5067-4233), 8-Position/18-Port Valve 1300 bar

Type Specification

Maximum pressure 1300 bar

Typical application 8 column selection

Port size Accepts M4 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Table 6 G4237A (5067-4279), 4-Position/10-Port Valve 800 bar

Type Specification

Maximum pressure 800 bar

Typical application 4 column selection

Port size Accepts M4 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Table 7 G4231C (5067-4241), 2-Position/6-Port Valve 1300 bar

Type Specification

Maximum pressure 1300 bar

Typical application Any two-way switching, e.g. between two detectors, between waste and detector, between two columns

Port size Accepts 10-32 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 37

2 Site Requirements and Specifications Valve Specifications

Table 8 G4231A (5067-4282), 2-Position/6-Port Valve 800 bar

Type Specification

Maximum pressure 800 bar

Typical application Any two-way switching, e.g. between two detectors, between waste and detector, between two columns

Port size Accepts 10-32 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Table 9 G4232D (5067-4240), 2-Position/10-Port Valve 1300 bar

Type Specification

Maximum pressure 1300 bar

Typical application Anything a 2-Position/6-Port valve can do plus alternating column regeneration

Port size Accepts 10-32 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Table 10 G4232C (5067-4283), 2-Position/10-Port Valve 800 bar

Type Specification

Maximum pressure 800 bar

Typical application Anything a 2-Position/6-Port valve can do plus alternating column regeneration

Port size Accepts 10-32 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 38

2 Site Requirements and Specifications Valve Specifications

Table 11 G4234C (5067-4273), 6-Position/14-Port Valve 1300 bar

Type Specification

Maximum pressure 1300 bar

Typical application 6 column selection

Port size Accepts M4 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Table 12 G4234A (5067-4284), 6-Position/14-Port Valve 800 bar

Type Specification

Maximum pressure 800 bar

Typical application 6 column selection

Port size Accepts M4 male threaded fittings

Liquid contacts PEEK, Stainless steel

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Table 13 G5641A (5067-6682), 2-position/10-port Bio valve head 1300 bar

Type Specification

Maximum pressure 1300 bar

Typical application Anything a 2-position/6-port valve can do plus alternating column regeneration

Port size Accepts 10-32 male threaded fittings

Liquid contacts PEEK, MP35N

pH range 0 141

1 incompatible with some mineral acids. For more information see Solvent Information.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 39

2 Site Requirements and Specifications Extended Specifications

Extended Specifications

The G7116A MCT is delivered with Large ID Quick Connect Heat Exchanger (0.17 mm with 3 L internal volume) that is suitable for standard applications.

The G7116B MCT is delivered with Low Dispersion Quick Connect Heat Exchanger (0.12 mm with 1.6 L internal volume) that is suitable for standard applications. Additional flavors of Quick Connect Heat Exchangers are available for optimization regarding better heating performance at higher flow rates (>2.5 mL) or for reducing the dispersion volume for low flow applications.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 40

3 Using the Module

Magnets 42

Turn on/off 43

Status Indicators 45

Open the Front Door 46

Install Heat Exchanger 47

Exchange a Column 51

Installing Valve Heads 54 Remove the Transportation Lock and the Valve Dummy 54 Install the Valve Head and Connect Capillaries 56

Installing the capillaries 60

Install the Divider Assembly 62

Install the Thermal Equilibration Device 63

Installing and Using Column ID Tags 65 Install the Column Identification Option 65 Connecting the Column ID Tags 69 Using Column ID Tags 70 Availability of Column Information 78

Agilent Local Control Modules 81

This chapter gives instructions on how to use the module.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 41

3 Using the Module Magnets

Magnets

1 Magnet in the front door of the Multicolumn Thermostat.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 42

3 Using the Module Turn on/off

Turn on/off

This procedure exemplarily shows an arbitrary LC stack configuration.

1 2

Power switch: On

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 43

3 Using the Module Turn on/off

3 Turn instrument On/Off with the control software. 4

Power switch: Off

5

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 44

3 Using the Module Status Indicators

Status Indicators

This procedure exemplarily shows an arbitrary LC stack configuration.

1 The module status indicator indicates one of six possible module conditions:

Status indicators

1. Idle

2. Run mode

3. Not-ready. Waiting for a specific pre-run condition to be reached or completed.

4. Error mode - interrupts the analysis and requires attention (for example, a leak or defective internal components).

5. Resident mode (blinking) - for example, during update of main firmware.

6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 45

3 Using the Module Open the Front Door

Open the Front Door

The front door opens in two angles: 90 and 180. In the 90 position, it can be used as a tray.

1 Press the release button to open the front door. 2 Open the door in a 90 angle to use it as a tray.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 46

3 Using the Module Install Heat Exchanger

Install Heat Exchanger

Modified design of Quick Connect Heat Exchangers allows simplified installation of the heat exchanger in the Multicolumn Thermostat (MCT).

New design Quick Connect Heat Exchangers do not have twist lock clips on the front side of the heat exchanger. Instead, Quick Connect Heat Exchangers include two column holders to fix the heat exchanger in the MCT heater block.

Figure 10 Quick Connect Heat Exchangers of the old design (top, A) and new design (bottom, B)

For bio-inert modules use bio-inert parts only!

For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.

A

B

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 47

3 Using the Module Install Heat Exchanger

Tools required p/n Description

5043-0915A Fitting mounting tool

5023-2502A Hex driver SW-6.35, slitted

8710-0510A Open-end wrench 1/4 5/16 inch

Parts required p/n Description

For 1290:

G7116-60015A Quick Connect Heat Exchanger Standard

G7116-60021A Quick Connect Heat Exchanger Ultra Low Dispersion

G7116-60031A Quick Connect Heat Exchanger High Flow

5067-5957A InfinityLab Quick Connect Assy ST 0.12 mm x 105 mm (from heat exchanger to column)

G7116-68003A Column Holder Lamella, 2/pk

For 1260:

G7116-60061A Quick Connect Heat Exchanger Large ID High Flow

5500-1193A InfinityLab Quick Turn Capillary ST 0.17 mm x 105 mm, long socket (from heat exchanger to column)

G7116-60051A Quick Connect Heat Exchanger Large ID

5067-5966A InfinityLab Quick Turn Fitting

G7167-68703A Fitting Intermediate Kit

G7116-68003A Column Holder Lamella, 2/pk

Bio-inert option:

G7116-60041A Quick Connect Heat Exchanger Bio-inert

5067-4780A Bio Capillary 0.17 mm x 300 mm, 1.6 mm OD socket (from column to detector)

0100-1516A Finger-tight fitting PEEK, 2/pk

5067-4741A ZDV union (Bio-inert)

5067-5403A UHP fitting

G7116-68003A Column Holder Lamella, 2/pk

Bio-compatible option:

G7116-60071A Quick Connect Bio Heat Exchanger Standard Flow

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 48

3 Using the Module Install Heat Exchanger

G7116-60081A Quick Connect Bio Heat Exchanger High Flow

G7116-68003A Column Holder Lamella, 2/pk

5500-1596A Quick Turn Capillary MP35N 0.12 mm x 280 mm

5067-5966A InfinityLab Quick Turn Fitting

5500-1578A Quick Connect Capillary MP35N 0.12 mm x 105 mm

5067-5965A InfinityLab Quick Connect LC fitting

1 Possible positions for heat exchangers. If only one column is used, the preferred positions are 2, 3, 6, or 7.

2 Connect the sampler outlet capillary (or the capillary from the column selection valve) to the inlet port of the heat exchanger.

3 Connect the column connection capillary to the outlet of the Quick Connect Heat Exchanger.

4 Position the heat exchanger in the groove of the MCT heater block and prepare column holders to fix the heat exchanger.

Parts required p/n Description

5 6 7 8

1 2 3 4

Max. 2.5 Nm

Max. 2.5 Nm

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 49

3 Using the Module Install Heat Exchanger

5 Turn column holders to fix the Quick Connect Heat Exchanger in the MCT.

NOTE The column holder clip can be mounted at any free spot on top of the heat exchanger.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 50

3 Using the Module Exchange a Column

Exchange a Column

Tools required p/n Description

5043-0915A Fitting mounting tool

8710-0510A Open-end wrench 1/4 5/16 inch

Parts required p/n Description

For 1290:

5067-5957A InfinityLab Quick Connect Assy ST 0.12 mm x 105 mm (from heat exchanger to column inlet)

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket (from column to detector)

5067-5966A InfinityLab Quick Turn Fitting

For 1260:

5500-1193A InfinityLab Quick Turn Capillary ST 0.17 mm x 105 mm, long socket (from heat exchanger to column)

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket (from column to detector)

5067-5966A InfinityLab Quick Turn Fitting (column inlet)

0100-1516A Finger-tight fitting PEEK, 2/pk (column outlet)

Bio-inert option:

5067-4780A Bio Capillary 0.17 mm x 300 mm, 1.6 mm OD socket (from column to detector)

5067-5966A InfinityLab Quick Turn Fitting (column inlet)

0100-1516A Finger-tight fitting PEEK, 2/pk (column outlet)

Bio-compatible option:

5067-5966A InfinityLab Quick Turn Fitting

5500-1596A Quick Turn Capillary MP35N 0.12 mm x 280 mm

5500-1578A Quick Connect Capillary MP35N 0.12 mm x 105 mm

5067-5965A InfinityLab Quick Connect LC fitting

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 51

3 Using the Module Exchange a Column

Remove a column

Preparations Heat exchanger(s) installed in MCT Column holder clip(s) installed in the heater block on top of heat exchanger(s) Column inlet capillary connected to heat exchanger outlet

1 Remove the column from the column holder. 2 Disconnect the column outlet.

3 Open the lever of the Quick Connect Fitting. 4 Unscrew and remove the Quick Connect Fitting from the column inlet.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 52

3 Using the Module Exchange a Column

Install a column

1 Connect the column inlet capillary to the column inlet using the Quick Connect Fitting.

2 Turn the column onto the Quick Connect Fitting until it is finger-tight.

3 Close the lever to make a leak-tight connection. 4 Connect the capillary to detector to the column outlet. When using the Quick Turn Fitting, tighten the fitting until it is finger-tight.

5 Use a wrench to counter the column while tightening the capillary fitting.

6 Position the column into the column holder clip(s).

7 Configure the column. For further information see Agilent Information Center or the online help of your CDS.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 53

3 Using the Module Installing Valve Heads

Installing Valve Heads

If ordered, the valve drives are factory-installed in the Multicolumn Thermostat. The valve heads are interchangeable and can be easily mounted.

At the first installation, the transportation lock and the dummy valve have to be removed, see Remove the Transportation Lock and the Valve Dummy on page 54. The valve heads can be installed by mounting the valve heads onto the valve drives and fastening the nut manually (do not use any tools).

Be sure that the guide pin snaps into the groove of the valve drive thread.

Remove the Transportation Lock and the Valve Dummy The following procedure demonstrates the necessary steps for installing the valve head to the valve drive of a Multicolumn Thermostat (MCT).

For the installation of a valve head to a G1170A Valve Drive you can ignore the steps that describe the MCT features of the transportation lock and spring loaded valve drive.

NOTE The valves are mounted on pull-out rails to allow easy installation of capillaries. Push the valve gently into its housing until it snaps into the inner position, push it again and it slides out.

When all capillaries are installed, push the valve back into its housing, see Install the Valve Head and Connect Capillaries on page 56.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 54

3 Using the Module Installing Valve Heads

1 Switch off the module. 2 When unscrewing the transportation lock, push it back until the last screw is removed - the valve rail is spring-loaded.

3 Press on the valve dummy (1.) to release it (2.) (spring-loaded valve rail).

4 Unscrew the valve dummy.

5 Remove the valve dummy from the valve drive.

1.

2.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 55

3 Using the Module Installing Valve Heads

Install the Valve Head and Connect Capillaries

For bio-inert modules use bio-inert parts only!

For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.

CAUTION The valve actuator contains sensitive optical parts, which need to be protected from dust and other pollution. Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results.

Always install a valve head for operation and storage. For protecting the actuator, a dummy valve head can be used instead of a functional valve. Do not touch parts inside the actuator.

CAUTION Column Damage or Bias Measurement Results

Switching the valve to a wrong position can damage the column or bias measurement results.

Fit the lobe to the groove to make sure the valve is switched to the correct position.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 56

3 Using the Module Installing Valve Heads

CAUTION Valve Damage

Using a low pressure valve on the high pressure side can damage the valve.

When using multiple column compartments as part of a method development solution, make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector.

NOTE For information about the compatibility mode of 800 bar valve heads see Information on RFID Tag Technical Note (01200-90134).

NOTE For a correct installation of the valve head, the outside pin (red) must completely fit into the outside groove on the valve drives shaft (red). A correct installation is only possible if the two pins (green and blue) on the valve head fit into their corresponding grooves on the valve drives actuator axis. Their match depends on the diameter of the pin and groove.

NOTE The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module. Valve properties will not be updated, if the valve head is replaced while the module is on. Selection of valve port positions can fail, if the instrument does not know the properties of the installed valve.

NOTE To allow correct valve identification, power off the valve drive for at least 10 s.

NOTE For firmware requirements see Information on RFID Tag Technical Note (01200-90134) which is included to each valve head.

CAUTION Sample degradation and contamination of the instrument

Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination.

For bio applications, always use dedicated bio parts, which can be identified by the bio-inert symbol or other markers described in this manual.

Do not mix bio, and non-bio modules or parts in a bio system.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 57

3 Using the Module Installing Valve Heads

1 Insert the valve head into the valve shaft.

OR If the outside pin does not fit into the outside groove, you have to turn the valve head until you feel that the two pins snap into the grooves. Now you should feel additional resistance from the valve drive while continuously turning the valve head until the pin fits into the groove.

2 When the outer pin is locked into the groove, manually screw the nut onto the valve head.

NOTE Fasten the nut manually. Do not use any tools.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 58

3 Using the Module Installing Valve Heads

3 Install all required capillary connections to the valve. 4 Push the valve head until it snaps in and stays in the rear position.

5 Power on or power-cycle your module, so the valve head gets recognized during module initialization.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 59

3 Using the Module Installing the capillaries

Installing the capillaries

The 2-position/10-port valve can be used here in the same way as a 2-position/6-port valve, just follow the rerouting diagram below.

Map the ports from the 2-position/6-port valve to the corresponding ports of the 2-position/10-port valve according to the red arrows. For example, mount the capillary connected to port 6 (2-position/6-port) at port 2 instead.

Connect port 1 and port 8 with a 120 mm length capillary. Plug plastic fittings into ports 9 and 10.

Figure 11 Rerouting of 2-position/10-port valve to match 2-position/6-port valve

1 Connect the capillaries going to a valve and fasten them immediately with a spanner.

2 Starting from position one through six (ten, respectively), connect respective capillaries to the heat exchangers. First, finger-tighten and then use a spanner.

3 Finger-tighten the capillaries going from heat exchangers to columns on the heat exchanger side, then fasten all fittings with a spanner.

4 Mount heat exchangers into the MCT.

5 Connect column inlet capillaries to the columns.

6 Connect column outlet capillaries to the columns.

1 2

3

4 5

6 8

1 2 3

4 5 6 7

9 10 Plastic fitting

120 mm length capillary

NOTE Use utmost care to avoid any void volumes caused by poor connections.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 60

3 Using the Module Installing the capillaries

7 Fasten all fittings on attached modules (autosampler, detector, additional pumps). Fit all unused valve ports with blank nuts.

8 Push the valve into the rear position.

9 Place the capillaries that go to another module or waste into the capillary guides to prevent squeezing them when closing the front cover.

10 Stow any excess lengths of the capillaries.

11 Perform a final leak check.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 61

3 Using the Module Install the Divider Assembly

Install the Divider Assembly

Parts required p/n Description

G7116-60006A Divider Assembly MCT

NOTE The Divider Assembly must be installed if different temperatures are used on the right and the left heater element to separate these two temperature zones.

1 Push the rear part of the Divider Assembly into position (1.) and fix it with the screw (2.).

2 Install the valve head if used.

See Replace Valve Heads on page 135.

3 Install all capillaries and heat exchangers required.

For details, refer to Install Heat Exchanger on page 47, and Exchange a Column on page 51.

4 Install the front part of the Divider Assembly by putting it on top.

5 The front plate should be flush with the top and the bottom edge of the MCT housing.

1.

2.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 62

3 Using the Module Install the Thermal Equilibration Device

Install the Thermal Equilibration Device

The InfinityLab Thermal Equilibration Device occupies the top row of the Multicolumn Thermostat (MCT) (1, 5). Therefore, Quick Connect Heat Exchangers and columns cannot be installed in the top row. Up to 6 columns can be installed in the MCT when two InfinityLab Thermal Equilibration Devices are installed at the same time.

5 6 7 8

1 2 3 4

Tools required p/n Description

5023-2502A Hex driver SW-6.35, slitted

Preparations Install Quick Change Valve Head and columns according to the instructions in Multicolumn Thermostat User Manual.

NOTE Divider assembly cannot be installed in the MCT together with the Thermal Equilibration Device at the same time.

WARNING Hot Surfaces

When an InfinityLab Thermal Equilibration Device is installed, it might be hot.

Allow the InfinityLab Thermal Equilibration Device to cool down before removing it and performing any procedures with column/valve installation/deinstallation.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 63

3 Using the Module Install the Thermal Equilibration Device

1 Check that the column in use is no longer than 30 cm.

2 Place the InfinityLab Thermal Equilibration Device covering one or more installed columns in the way that the twist-lock clips are located at the top of the device.

3 Guide capillaries (and column ID tag cords, if installed) through silicone side covers.

4 To avoid obstruction of the capillary guidance, remove the side covers of the InfinityLab Thermal Equilibration Devices that point towards the valve.

5 Turn twist-lock clips with the hex-driver to fix the InfinityLab Thermal Equilibration Device in place.

6 Guide the capillaries through the recesses provided at the top and bottom of the module.

7 Close the MCT door.

8 Let the system equilibrate before beginning the analysis.

NOTE This measure is only necessary if several columns are connected to the column selection valve and two InfinityLab Thermal Equilibration Devices are installed in one MCT.

NOTE This measure prevents the capillaries from being squeezed through the doors.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 64

3 Using the Module Installing and Using Column ID Tags

Installing and Using Column ID Tags

Install the Column Identification Option

Parts required p/n Description

5067-5915A Column ID Kit Left

5067-5916A Column ID Kit Right

5067-5917A InfinityLab Column Identification Tag

Preparations Power off the instrument.

CAUTION Electronic boards and components are sensitive to electrostatic discharge (ESD).

ESD can damage electronic boards and components.

Be sure to hold the column ID modules by the plastic parts, and do not touch the electrical components. Do not touch the pins of the flex-board connector.

NOTE In the Agilent 1260 Infinity II Multicolumn Thermostat (G7116A), the column ID module is installed on the left side of the MCT only.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 65

3 Using the Module Installing and Using Column ID Tags

1 Remove any tube guides and tube clip holders that may already be installed on the sides of the MCT cover.

2 Unlock the left and right (G7116B only) side cover inserts by pushing them to the rear and put them aside.

3 Identify the left and right (G7116B only) column ID module. The ID sockets 1 to 4 are numbered from top to bottom.

4 Take the end of the preinstalled flex-board connector out of the holder.

1.

2.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 66

3 Using the Module Installing and Using Column ID Tags

5 Connect the flex cable to the column ID module.

a Open the locking mechanism of the connector by lifting up the frame.

b Push in the cable with the contacts facing to the rear.

c Close the locking mechanism.

d Verify that the cable is properly attached to the connector without visible offset.

6 Attach the column ID module to the MCT cover.

1.

2.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 67

3 Using the Module Installing and Using Column ID Tags

7 Repeat steps 4 to 9 for the column ID module on the other side (for G7116B, on the right).

8 Install the waste tube clip holder (example shows a G7116B).

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 68

3 Using the Module Installing and Using Column ID Tags

Connecting the Column ID Tags

1 If the column has no Column ID Tag, fit a tag by slipping the loop over one end of the column and pulling it tight through the plastic holder.

NOTE Once the loop has been pulled tight, the tag can no longer be removed from the column.

2 Install the column in the column compartment, see Exchange a Column on page 51.

3 Plug the free end of the Column ID Tag into the adjacent socket in the column tag reader unit.

NOTE It is important that the Column ID Tag is plugged into the adjacent socket in a logical order (that is, first column from the top into "1", second column from the top into "2", and so on). Otherwise, it is easy to confuse the columns and their locations during column assignment in a CDS.

NOTE If you have a full-length column, we recommend that you use the adjacent socket on the left column tag reader.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 69

3 Using the Module Installing and Using Column ID Tags

Using Column ID Tags The Agilent InfinityLab Column Identification Tag is designed to enable the automatical detection of the columns installed in the column thermostat and help the user to track the usage of the columns in the laboratory. The column identification tag contains information about not only the physical properties of the column but also about its usage. Two types of information are stored:

Static fields contain information on the physical characteristics of the column such as the length, internal diameter and particle size. Typically, this information stays the same for the lifetime of the column.

Dynamic fields contain information on the usage of the column, such as the number of injections and maximum measured temperature. The information stored in the dynamic fields will be automatically updated each time a new analysis is performed with the column. These fields are read-only from the perspective of the user, meaning that their content cannot be manually edited or erased.

For pre-labeled Agilent columns, the static fields are pre-populated with information and set to read-only for compliance reasons, except for the Void Volume and Comment fields. The dynamic fields are blank by default and will be automatically edited by the system upon using the column.

On the contrary, custom column identification tags enable the user to edit the content for all static fields, as long as the tag is not sealed. This can be beneficial when using non-Agilent columns. After entering all the necessary information, the custom tag can be sealed to prevent any further modification of the content in the static fields. The content of the dynamic fields cannot be manually edited or erased, not even for the custom tags.

NOTE The information in the dynamic fields cannot be erased by any means.

NOTE When the column identification tag is sealed, the static fields will become write-protected and only the dynamic fields will remain available for updating. The only exceptions from this are the static fields Void Volume and Comment.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 70

3 Using the Module Installing and Using Column ID Tags

There are two ways to edit the content of custom column identification tags:

One way is to type in all the necessary information manually, using the Column Assignment tab of the control interface.

The other one is to import the data from a database (an existing catalog or inventory, or the Agilent columns guide). This option is available only for the OpenLab CDS ChemStation (C.01.07 SR2 or higher).

When plugging a new tag into the tag reader, the content that is stored on it will be automatically imported into the Edit Columns tab (only for OpenLab CDS ChemStation, see Using Column ID Tags with ChemStation on page 72 for more information) and the Column Assignment tab of the control interface.

NOTE It may take up to several seconds for the column tag to be read and the tables to be updated.

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3 Using the Module Installing and Using Column ID Tags

Using Column ID Tags with ChemStation

1 In the Method and Run Control view of the Agilent OpenLAB CDS ChemStation Edition, select Columns from the Instrument menu.

The Edit Columns dialog box is displayed.

If the Edit Columns table is empty, go straight to Step 3.

Figure 12 The ChemStation Edit Columns table

2 Select a line in the table that contains column information as close as possible to the column you are adding. The selected line acts as a template for the new column.

3 Click the Insert button to insert a line above the currently selected line, or the Append button to add a line to the end of the table.

The new line contains a copy of the information in the template line.

4 Click the Edit button to display a dialog box that allows you to edit the column-specific information such as Serial Number, Batch Number and Description.

5 Add or edit the other column-specific information (for example, maximum pressure, maximum temperature, length, diameter, particle size) in the Edit Columns table.

6 If the column is installed and will be used in the Multicolumn Thermostat, select YES in the Installed column.

NOTE Column catalogs are available only in OpenLab CDS ChemStation Edition C.01.07 SR2 and above with drivers A.02.14 and above. Click the Catalog button to display a dialog box that allows you to choose how to load the catalog into the table.

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3 Using the Module Installing and Using Column ID Tags

7 Click the Plumbing button.

The Column Assignment dialog box is displayed.

Figure 13 Column Assignment dialog box

The Column Assignment dialog box allows you to specify and review detailed information about the columns attached to each position in the column compartment. The information in the Column Assignment dialog box is in three sections:

1 The Plumbing section contains a table where you can specify the plumbing assignment for each valve position.

2 The Visualization section gives a visual representation of the Multicolumn Thermostat configuration.

3 The Column Tag Information table shows the information stored on the column tag(s) of the installed column(s). For more details, see The Column Tag Information Table on page 79.

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3 Using the Module Installing and Using Column ID Tags

8 Click in the Import column of an empty line in the Column Tag Information table.

The list of columns from the ChemStation's Edit Columns table is displayed.

Figure 14 The list of columns from the ChemStation's Edit Columns table

To reduce the list to only those columns that are marked as Installed, mark the Only show installed Columns check box.

9 Select the column information to import from the list of columns and click OK.

The column information is imported into the Column Tag Information table.

NOTE It may take several seconds before the information appears in the Column Tag Information table.

NOTE The characters semicolon (;), single quote (') and double quote (") are invalid for the Column Tag Information table. If these characters are used in any field of the table, an error is displayed, and OK/Write Tag button is disabled. You must delete all invalid characters before you can write the data to the tag.

The Description and Comment fields are limited to 32 characters in the Column Tag Information table.

NOTE At this stage, you can assign a color to the column using the drop-down list in the Color Code column; this information is displayed in the Visualization panel, but is not written to the tag.

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3 Using the Module Installing and Using Column ID Tags

10 Click the >> button at the top right of the Column Tag Information table to show the hidden table columns. Use the horizontal scroll bar to access the columns at the right of the table.

11 If all the information for the column is correct, click the Ok/Write Tag button to write the information to the column ID tag.

The information in the ChemStation's Edit Columns table is also updated.

12 You can edit the information on the column ID tag using the ChemStation Edit Columns table. When you have finished editing the information, repeat steps 7 to 9 to update the information in the tag.

13 When you are sure that you will not make any more changes to the information in the tag, you can irrevocably seal the tag to set all static fields to read-only. Right-click in the appropriate line in the Column Tag Information table and select Seal Column Tag from the context menu.

The sealed column is shown in the Column Tag Information table with the icon at the beginning of the row. In the Edit Columns table of the ChemStation, it is shown with Sealed in the Tag column.

Figure 15 The Column Tag Information table context menu

NOTE Once a column ID tag has been sealed, the static fields cannot be edited.

Until the column ID tag has been sealed, you can delete all information in static fields on the tag using the Clear Column Tag Information command from the context menu.

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The Plumbing button of the ChemStation Edit Columns table displays the Column Assignment dialog box. It can also be displayed by selecting Column Assignment from the context menu of the column compartment Dashboard panel in the ChemStation's Method and Control view.

Figure 16 Column Assignment dialog box overview

The Column Assignment dialog box has three sections that give you information about the column:

The Plumbing section shows the valve connections to the positions in the column compartment.

The Visualization section gives a visual representation of the configuration of the columns in the column compartment; the columns are color coded.

Place the mouse cursor over a column to display a tooltip of the column information from the column ID tag.

Click a column to highlight the column information in the Column Tag Information table.

The Column Tag Information shows the information in the column ID tags for all columns in the configuration, including their location in the column compartment and their color codes.

NOTE Make the connections to give the shortest distances between the valve ports and the columns, and use a logical order (left column 1 to port 1-1', left column 2 to port 2-2' and so on). Avoid leaving unused ports between used ones.

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3 Using the Module Installing and Using Column ID Tags

The column compartment panel of the Dashboard in the ChemStation's Method and Control view also allows you a quick view of the column ID tag information.

Figure 17 The dashboard panel of the column compartment

Place the mouse cursor over the column ID tag icon ( ); the tooltip shows the information currently stored on the column ID tag.

The column ID tag icon changes according to its state as described in The Column Tag Information Table on page 79.

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Availability of Column Information

The ChemStation Columns Table

The Edit Columns dialog box, which you access using the Columns command of the Instrument menu in Method and Run Control view, shows the detailed information about the columns attached to each position in the device. The table contains all the column-specific information stored in the column ID tags (see Column Tag Information on page 80) plus the following possible additional columns:

The table includes not only the columns that are installed in the column thermostat device(s), but also the inventory of other available columns, for example, columns that have been used in the past. The ChemStation also provides a catalog of column types, which you can load into the Edit Columns table to act as templates for other columns.

Installed YES when the column is installed in a device. no when the column is not installed in a device.

Location Shows the location in the device to which the plumbing of the valve position leads.

Dev. Serial# Present only for a valve thermostat cluster. Shows the serial number of the device that contains the column.

Tag Shows if the column has column ID tag (Used), if it sealed (Sealed) or the column has no tag (empty).

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3 Using the Module Installing and Using Column ID Tags

The Column Tag Information Table

The Column Assignment dialog box, which you access using the Column Assignment command of the dashboard context menu, shows the detailed information about the columns attached to each position in the device. The dialog box includes the Column Tag Information table, which contains all the column-specific information stored on the column ID tags (see Column Tag Information on page 80) plus the following possible additional columns:

By default, only the Column Tag Information table columns up to and including the Injection column are displayed. Click the >> button at the top right of the table to show the full table.

Tag Status Shows the status of the column ID tag: Empty: The position is empty or has a column without a column

ID tag.

: A column with a column ID tag is installed at this position.

: A column with a sealed column ID tag is installed at this position.

: A column with a prelabeled column ID tag supplied by Agilent Technologies is installed at this position.

Location Shows the location in the device to which the plumbing of the valve position leads. For a valve thermostat cluster, the Column Host (the device where the column is installed) is also shown.

Color Code Shows the color representing the column currently occupying the valve position.

Import Present only when the CDS is an Agilent OpenLab CDS ChemStation Edition.

Click the icon to display a dialog box listing all the columns entered in the ChemStation's Edit Columns table, from which you can select appropriate information to import.

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Column Tag Information

The column ID tag contains the following information:

Field Description Type Read/Write permission pre-labeled tag

Read/Write permission custom tag

Description A description of the column. Static Read Write

Length The length of the column in mm. Static Read Write

Diameter The internal diameter of the column in mm. Static Read Write

Particle Size The particle size of the column packing material in m.

Static Read Write

Maximum Pressure The maximum pressure supported by the column.

Static Read Write

Number of Injections The number of injections that have been made on the column.

Dynamic Read Read

Product Number The product number of the column. Static Read Write

Maximum Measured Temperature

The highest temperature (in C) experienced by the column to date.

Dynamic Read Read

Maximum Temperature

The safe maximum operating temperature of the column (in C).

Static Read Write

Minimum pH The minimum pH supported by the column. Static Read Write

Maximum pH The maximum pH supported by the column. Static Read Write

Void Volume (mL) The void volume of the column and fittings. Static Write Write

First Injection The date and time of the first injection onto the column.

Dynamic Read Read

Recent Injection The date and time of the most recent injection onto the column.

Dynamic Read Read

Manufacturing Date The date of manufacture of the column. Static Read Write

Agilent Column Whether or not the column was supplied by Agilent Technologies.

Static Read Write

Serial Number The serial number of the column. Static Read Write

Batch Number The batch number of the column. Static Read Write

Tag Sealed Whether or not all static fields except Comment and Void Volume are set irrevocably to read-only.

Static Read Write

Comment A user-generated comment about the column. Static Write Write

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3 Using the Module Agilent Local Control Modules

Agilent Local Control Modules

Agilent InfinityLab Companion G7108AA

The Agilent InfinityLab Companion gives you complete control, system monitoring, signal plotting, and diagnostic capabilities for a wide range of LC system modules.

The instrument control solution is available as full package including all hardware and accessories, but can also be used on your own mobile devices like tablets, mobile phones and other electronic equipment.

Combining the conveniences of the Agilent Instant Pilot features with state-of-the-art mobile technology, the Agilent InfinityLab Companion gives you maximum flexibility and ease of use to control and monitor your LC system modules.

Features:

Complete local control and monitoring of Agilent Infinity II LC modules

Excellent usability and ease of use through a user interface specifically tailored for mobile devices - simple, intuitive touch-enabled, and visual controllable.

High flexibility through a modern Bring your own device approach. Connection between LC module and mobile device either wireless via Wi-Fi or wired over USB cable (with full package).

Convenient, ergonomic operation either handheld or attached to a module at the stack with newly developed, secure tablet holder (included in the full package).

Preconfigured tablet with all required software already installed (included in the full package).

Centerpiece of the solution is a USB dongle that activates the complete intelligence of the InfinityLab Companion on the instrument stack.

The InfinityLab Companion provides:

fast and direct control in front of the instrument

a clear overview of the system status

control functionalities

access to method parameters and sequences

a logbook showing events from the modules

diagnostic tests

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Leak and Waste Handling 83 Waste Concept 84

Solvent Information 85

Capillary Coding Guide 92 Syntax for Capillary Description 92 At-a-Glance Color-Coding Keys 94

Install Capillary Connections 95

This chapter provides information on how to set up the module for an analysis and explains the basic settings.

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Leak and Waste Handling

For details on correct installation, see separate installation documentation.

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 C (392 F). Do not use solvents with a boiling point below 56 C (133 F).

Avoid high vapor concentrations. Keep the solvent temperature at least 40 C (72 F) below the boiling point of the solvent used. This includes the solvent temperature in the sample compartment. For the solvents methanol and ethanol keep the solvent temperature at least 25 C (45 F) below the boiling point.

Do not operate the instrument in an explosive atmosphere.

Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

Reduce the volume of substances to the minimum required for the analysis.

Never exceed the maximum permissible volume of solvents (8 L) in the solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container.

Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

NOTE For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet.

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Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with

4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid.

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Solvent Information

Observe the following recommendations on the use of solvents.

Follow the recommendations for avoiding the growth of algae, see the pump manuals.

Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 m filters.

Avoid or minimize the use of solvents that may corrode parts in the flow path. Consider specifications for the pH range given for different materials such as flow cells, valve materials etc. and recommendations in subsequent sections.

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General Information about Solvent/Material Compatibility Materials in the flow path are carefully selected based on Agilents experiences in developing highest-quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.

Disclaimer

Subsequent data was collected from external resources and is meant as a reference. Agilent cannot guarantee the correctness and completeness of such information. Data is based on compatibility libraries, which are not specific for estimating the long-term life time under specific but highly variable conditions of UHPLC systems, solvents, solvent mixtures and samples. Information can also not be generalized due to catalytic effects of impurities like metal ions, complexing agents, oxygen etc. Apart from pure chemical corrosion, other effects like electro corrosion, electrostatic charging (especially for non-conductive organic solvents), swelling of polymer parts etc. need to be considered. Most data available refers to room temperature (typically 20 25 C, 68 77 F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials.

MP35N

MP35N is a nonmagnetic, nickel-cobalt-chromium-molybdenum alloy demonstrating excellent corrosion resistance (for example, against nitric and sulfuric acids, sodium hydroxide, and seawater) over a wide range of concentrations and temperatures. In addition, this alloy shows exceptional resistance to high-temperature oxidation. Due to excellent chemical resistance and toughness, the alloy is used in diverse applications: dental products, medical devices, nonmagnetic electrical components, chemical and food processing equipment, marine equipment. Treatment of MP35N alloy samples with 10 % NaCl in HCl (pH 2.0) does not reveal any detectable corrosion. MP35N also demonstrates excellent corrosion resistance in a humid environment. Although the influence of a broad variety of solvents and conditions has been tested, users should keep in mind that multiple factors can affect corrosion rates, such as temperature, concentration, pH, impurities, stress, surface finish, and dissimilar metal contacts.

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Polyphenylene Sulfide (PPS)

Polyphenylene sulfide has outstanding stability even at elevated temperatures. It is resistant to dilute solutions of most inorganic acids, but it can be attacked by some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids, such as sulfuric acid and phosphoric acid, have little effect on polyphenylene sulfide, but at high concentrations and temperatures, they can still cause material damage. Nonoxidizing organic chemicals generally have little effect on polyphenylene sulfide stability, but amines, aromatic compounds, and halogenated compounds may cause some swelling and softening over extended periods of time at elevated temperatures. Strong oxidizing acids, such as nitric acid (> 0.1 %), hydrogen halides (> 0.1 %), peroxy acids (> 1 %), or chlorosulfuric acid degrade polyphenylene sulfide. It is not recommended to use polyphenylene sulfide with oxidizing material, such as sodium hypochlorite and hydrogen peroxide. However, under mild environmental conditions, at low concentrations and for short exposure times, polyphenylene sulfide can withstand these chemicals, for example, as ingredients of common disinfectant solutions.

PEEK

PEEK (Polyether-Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is therefore the material of choice for UHPLC and biochemical instrumentation.

It is stable in the specified pH range (for the Bio-Inert LC system: pH 1 13, see bio-inert module manuals for details), and inert to many common solvents.

There is still a number of known incompatibilities with chemicals such as chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %, sulfuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogens or aqueous halogen solutions, phenol and derivatives (cresols, salicylic acid, and so on).

When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions, normal PEEK capillaries are sensitive to high pressure. Therefore, Agilent uses stainless steel cladded PEEK capillaries in bio-inert systems. The use of stainless steel cladded PEEK capillaries keeps the flow path free of steel and ensures pressure stability up to 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK.

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Polyimide

Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent.

Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like ammonia (e.g. ammonium salts in basic conditions) or acetates.

Polyethylene (PE)

Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow piston and wash seals, which are used in 1290 Infinity pumps, 1290 Infinity II pumps, the G7104C and for normal phase applications in 1260 Infinity pumps.

Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12.5. It is compatible with many organic solvents used in chromatographic systems like methanol, acetonitrile and isopropanol. It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar.

Tantalum (Ta)

Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides.

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Stainless Steel (SST)

Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by acids below pH 2.3. It can also corrode in following solvents:

Solutions of alkali halides, their respective acids (for example, lithium iodide, potassium chloride, and so on) and aqueous solutions of halogens.

High concentrations of inorganic acids like nitric acid, sulfuric acid and organic solvents especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel).

Halogenated solvents or mixtures which form radicals and/or acids, for example:

2 CHCl3 + O2 2 COCl2 + 2 HCl

This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.

Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, diisopropylether). Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides.

Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel.

Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid).

Mixtures of carbon tetrachloride with isopropanol or THF.

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Titanium (Ti)

Titanium is highly resistant to oxidizing acids (for example, nitric, perchloric and hypochlorous acid) over a wide range of concentrations and temperatures. This is due to a thin oxide layer on the surface, which is stabilized by oxidizing compounds. Non-oxidizing acids (for example, hydrochloric, sulfuric and phosphoric acid) can cause slight corrosion, which increases with acid concentration and temperature. For example, the corrosion rate with 3 % HCl (about pH 0.1) at room temperature is about 13 m/year. At room temperature, titanium is resistant to concentrations of about 5 % sulfuric acid (about pH 0.3). Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates. Titanium is sensitive to acidic metal chlorides like FeCl3 or CuCl2. Titanium is subject to corrosion in anhydrous methanol, which can be avoided by adding a small amount of water (about 3 %). Slight corrosion is possible with ammonia > 10 %.

Diamond-Like Carbon (DLC)

Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Fused silica and Quartz (SiO2)

Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows. It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides. It is corroded by strong bases and should not be used above pH 12 at room temperature. The corrosion of flow cell windows can negatively affect measurement results. For a pH greater than 12, the use of flow cells with sapphire windows is recommended.

Gold

Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia.

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Zirconium Oxide (ZrO2)

Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Platinum/Iridium

Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF)

Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons.

TFE/PDD copolymer tubings, which are used in all Agilent degassers except G1322A/G7122A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel. They have limited life time in the presence of hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let dry out the chamber. For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off.

The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible with the solvent DMF (dimethyl formamide).

Sapphire, Ruby and Al2O3-based ceramics

Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

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Capillary Coding Guide

Syntax for Capillary Description The tables below are your guide to identifying the proper specifications for your capillary. On all capillaries, dimensions are noted in id (mm), length (mm) and, where applicable, volume (L). When you receive your capillary, these abbreviations are printed on the packaging.

Using the guide: This fitting is coded as SPF, for Swagelok, PEEK, Fingertight.

Table 14 Capillary coding guide

Type The type gives some indication on the primary function, like a loop or a connection capillary.

Material The material indicates which raw material is used.

Fitting left/fitting right The fitting left/right indicate which fitting is used on both ends of the capillary.

Key Description Key Description Key Description

Capillary Connection capillaries

ST Stainless steel W Swagelok + 0.8 mm Port id

Loop Loop capillaries Ti Titanium S Swagelok + 1.6 mm Port id

Seat Autosampler needle seats

PK PEEK M Metric M4 + 0.8 mm Port id

Tube Tubing FS/PK PEEK-coated fused

silica1

E Metric M3 + 1.6 mm Port id

Heat exchanger

Heat exchanger PK/ST Stainless

steel-coated PEEK2

U Swagelok union

PFFE PTFE L Long

FS Fused silica X Extra long

MP35 N

Nickel-cobalt-chromi um-molybdenium alloy

H Long head

G Small head SW 4

N Small head SW 5

F Finger-tight

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V 1200 bar

B Bio

P PEEK

I Intermediate

1 Fused silica in contact with solvent

2 Stainless steel-coated PEEK

Table 14 Capillary coding guide

Type The type gives some indication on the primary function, like a loop or a connection capillary.

Material The material indicates which raw material is used.

Fitting left/fitting right The fitting left/right indicate which fitting is used on both ends of the capillary.

Key Description Key Description Key Description

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At-a-Glance Color-Coding Keys The color of your capillary will help you quickly identify the capillary id.

Table 15 Color-coding key for Agilent capillary tubing

Internal diameter in mm

Color code

0.015 Orange

0.025 Yellow

0.05 Beige

0.075 Black

0.075 MP35N Black with orange stripe

0.1 Purple

0.12 Red

0.12 MP35N Red with orange stripe

0.17 Green

0.17 MP35N Green with orange stripe

0.20/0.25 Blue

0.20/0.25 MP35N Blue with orange stripe

0.3 Grey

0.50 Bone White

HINT As you move to smaller-volume, high efficiency columns, youll want to use narrow id tubing, as opposed to the wider id tubing used for conventional HPLC instruments

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Install Capillary Connections

Capillaries and connections depend on which system is installed.

Table 16 Capillary connections for 1260 Infinity II systems

p/n From To

Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump

Capillary ST 0.17 mm x 500 mm SI/SI (5500-1246) Pump Sampler

Capillary, ST, 0.17 mm x 900 mm SI/SX (5500-1217) Pump Vialsampler with ICC

Capillary ST 0.17 mm x 500 mm SI/SI (5500-1246) Multisampler MCT Valve/Heat Exchanger

Capillary, ST, 0.17 mm x 400 mm SL/SL (5500-1252) Vialsampler MCT Valve/Heat Exchanger

Capillary ST 0.17 mm x 105 mm SL/SL (5500-1240) Vialsampler ICC Heat Exchanger

Capillary, ST, 0.17 mm x 120 mm SL/SL, long socket (5500-1250)

ICC Heat Exchanger Column

InfinityLab Quick Turn Capillary ST 0.17 mm x 105 mm, long socket (5500-1193)

MCT Heat Exchanger Column

InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket (5500-1191)

Column/MCT Valve Detector

Waste accessory kit (5062-8535) VWD Waste

Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462) DAD/FLD Waste

Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712) Detector Fraction Collector

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Table 17 Capillary connections for 1290 Infinity II systems

p/n From To

Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump

Capillary ST 0.17 mm x 400 mm SI/SI (5500-1245) Pump Sampler

Capillary, ST, 0.17 mm x 900 mm SI/SX (5500-1217) Pump Vialsampler with ICC

Capillary ST 0.12 mm x 500 mm SL/S (5500-1157) Multisampler MCT Valve/Heat Exchanger

Capillary ST 0.12 mm x 400 mm SL/SL (5500-1251) Vialsampler MCT Valve/Heat Exchanger

Capillary ST 0.12 mm x 105 mm SL/SL (5500-1238) Vialsampler ICC Heat Exchanger

Capillary ST 0.12 mm x 120 mm SL/SL, long socket (5500-1249)

ICC Heat Exchanger Column

Capillary ST 0.12 mm x 105 mm SL (5500-1201) MCT Heat Exchanger Column

InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket (5500-1191)

Column/MCT Valve Detector

Waste accessory kit (5062-8535) VWD Waste

Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462) DAD/FLD Waste

Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712) Detector Fraction Collector

Table 18 Capillary connections for 1260 Infinity II Bio-inert LC

p/n From To

Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump

Capillary Ti 0.17 mm x 500 mm, SL/SLV (5500-1264) Pump Multisampler

Capillary PK/ST 0.17 mm x 500 mm, RLO/RLO (Bio-inert) (G5667-81005)

Multisampler MCT

ZDV union (Bio-inert) (5067-4741) Capillary Bio-inert Heat Exchanger

Quick Connect Heat Exchanger Bio-inert (G7116-60041)

Capillary PEEK 0.18 mm x 1.5 m (0890-1763) and PEEK Fittings 10/PK (5063-6591)

Column/MCT Valve Detector

Waste accessory kit (5062-8535) VWD Waste

Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462) DAD/FLD Waste

Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712) Detector Fraction Collector

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4 Preparing the Module Install Capillary Connections

Table 19 Capillary connections for 1290 Infinity II Bio LC

p/n From To

Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump

Capillary MP35N 0.17 mm x 500 mm, SI/SI (5500-1419) Pump Multisampler

Capillary MP35N 0.12 mm x 500 mm SI/SI (5500-1279) Multisampler MCT

Quick Connect Capillary MP35N 0.12 mm x 105 mm (5500-1578)

MCT Heat Exchanger Column

Quick Turn Capillary MP35N 0.12 mm x 280 mm (5500-1596) Column/MCT Valve Detector (DAD)

Quick Turn Capillary MP35N 0.12 mm x 500 mm (5500-1598) Column/MCT Valve Detector (VWD)

Waste accessory kit (5062-8535) VWD Waste

Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462) DAD/FLD Waste

Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712) Detector Fraction Collector

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 97

4 Preparing the Module Install Capillary Connections

For correct installation of capillary connections it's important to choose the correct fittings, see Syntax for Capillary Description on page 92.

1 Select a nut that is long enough for the fitting you'll be using.

2 Slide the nut over the end of the tubing or capillary.

3 Carefully slide the ferrule components on after the nut and then finger-tighten the assembly while ensuring that the tubing is completely seated in the bottom of the end fitting.

4 Use a column or injection valve to gently tighten the fitting which forces the ferrule to seat onto the tubing or capillary.

NOTE Don't overtighten. Overtightening will shorten the lifetime of the fitting.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 98

4 Preparing the Module Install Capillary Connections

5 Loosen the nut and verify that the ferrule is correctly positioned on the tubing or capillary.

NOTE The first time that the swagelock fitting is used on a column or an injection valve, the position of the ferrule is permanently set. If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 99

5 Optimization

Optimizing the Column Compartment 101

This chapter provides information on how to optimize the Multicolumn Thermostat.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 100

5 Optimization Optimizing the Column Compartment

Optimizing the Column Compartment

For best performance results of the multicolumn compartment:

Use short connection capillaries and place them close to the heat exchanger. This reduces heat dissipation and excessive band broadening.

See the Accessories and Consumables section for additional available heat exchangers with various internal volumes to address certain applications in terms of flow rates and dispersion volume optimization.

Keep the left and right heat exchanger temperature the same unless you do specific applications.

Use the Divider Assembly, which is a part of the Accessory Kit for G7116B, whenever you work with different temperatures on the left and right heater element to verify an optimized separation of both temperature zones. See also Install the Divider Assembly on page 62.

Keep the front cover closed during analysis.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 101

6 Troubleshooting and Diagnostics

Available Tests vs User Interfaces 103

Agilent Lab Advisor Software 104

Overview about the troubleshooting and diagnostic features.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 102

6 Troubleshooting and Diagnostics Available Tests vs User Interfaces

Available Tests vs User Interfaces

Depending on the user interface, the available tests and the screens/reports may vary (see chapter "Test Functions and Calibrations").

Preferred tool should be the Agilent Lab Advisor software, see Agilent Lab Advisor Software on page 104.

The Agilent ChemStation may not include any maintenance/test functions.

Screenshots used within these procedures are based on the Agilent Lab Advisor software.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 103

6 Troubleshooting and Diagnostics Agilent Lab Advisor Software

Agilent Lab Advisor Software

The Agilent Lab Advisor Software (basic license, shipped with an Agilent LC pump) is a standalone product that can be used with or without a chromatographic data system. Agilent Lab Advisor helps to manage the lab for high-quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status, Early Maintenance Feedback counters (EMF), instrument configuration information, and diagnostic tests. With the push of a button, a detailed diagnostic report can be generated. Upon request, the user can send this report to Agilent for a significantly improved troubleshooting and repair process.

The Agilent Lab Advisor software is available in two versions:

Lab Advisor Basic

Lab Advisor Advanced

Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument.

The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines. In addition, all diagnostic test results, calibration results, and acquired signal data can be uploaded to a shared network folder. The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated. This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems.

The optional Agilent Maintenance Wizard Add-on provides an easy-to-use, step-by-step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity LC Series instrument.

The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 104

7 Error Information

What Are Error Messages 106

General Error Messages 107 Timeout 107 Shutdown 108 Remote Timeout 109 Lost CAN Partner 109 Leak 110 Leak Sensor Open 111 Leak Sensor Short 111 Compensation Sensor Open 112 Compensation Sensor Short 112

MCT Error Messages 113 VALVE INIT FAILED 113 VALVE FAILED 114 VALVE TAG VIOLATION 114 WRONG VALVE 115 WAIT TIMEOUT 116 HEATEX OVERTEMP 116 UHX SENSOR ERROR 116 LHX SENSOR ERROR 117 LHS SENSOR ERROR 117 PELTIER OVERCURRENT 118 LEFT PELTIER ERROR 118 RIGHT PELTIER ERROR 118

This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 105

7 Error Information What Are Error Messages

What Are Error Messages

Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook.

If an error occurs outside a method run, other modules will not be informed about this error. If it occurs within a method run, all connected modules will get a notification, all LEDs get red and the run will be stopped. Depending on the module type, this stop is implemented differently. For example, for a pump the flow will be stopped for safety reasons. For a detector, the lamp will stay on in order to avoid equilibration time. Depending on the error type, the next run can only be started, if the error has been resolved, for example liquid from a leak has been dried. Errors for presumably single time events can be recovered by switching on the system in the user interface.

Special handling is done in case of a leak. As a leak is a potential safety issue and may have occurred at a different module from where it has been observed, a leak always causes a shutdown of all modules, even outside a method run.

In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface).

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 106

7 Error Information General Error Messages

General Error Messages

General error messages are generic to all G HPLC modules.

Timeout Error ID: 0062

The timeout threshold was exceeded.

Probable cause Suggested actions

1 The analysis was completed successfully,

and the timeout function switched off the module as requested.

Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required.

2 A not-ready condition was present during a sequence or multiple-injection run for a period longer than the timeout threshold.

Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 107

7 Error Information General Error Messages

Shutdown Error ID: 0063

An external instrument has generated a shutdown signal on the remote line.

The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.

Probable cause Suggested actions

1 Leak detected in another module with a CAN connection to the system.

Fix the leak in the external instrument before restarting the module.

2 Leak detected in an external instrument with

a remote connection to the system.

Fix the leak in the external instrument before restarting the module.

3 Shut-down in an external instrument with a

remote connection to the system.

Check external instruments for a shut-down condition.

4 The degasser failed to generate sufficient vacuum for solvent degassing.

Check the vacuum degasser for an error condition. Refer to the Service Manual for the degasser or the pump that has the degasser built-in.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 108

7 Error Information General Error Messages

Remote Timeout Error ID: 0070

A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis. If a not-ready condition is still present on the remote line after one minute the error message is generated.

Lost CAN Partner Error ID: 0071

During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed.

The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.

Probable cause Suggested actions

1 Not-ready condition in one of the instruments connected to the remote line.

Ensure the instrument showing the not-ready condition is installed correctly, and is set up correctly for analysis.

2 Defective remote cable. Exchange the remote cable.

3 Defective components in the instrument

showing the not-ready condition.

Check the instrument for defects (refer to the instruments documentation).

Probable cause Suggested actions

1 CAN cable disconnected. Ensure all the CAN cables are connected correctly.

Ensure all CAN cables are installed correctly.

2 Defective CAN cable. Exchange the CAN cable.

3 Defective mainboard in another module. Switch off the system. Restart the system, and determine which module or modules are not recognized by the system.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 109

7 Error Information General Error Messages

Leak Error ID: 0064

A leak was detected in the module.

The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent. This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board.

Probable cause Suggested actions

1 Condensation. Use a higher temperature setpoint.

2 Loose column fittings. Ensure all fittings are tight.

3 Broken capillary. Exchange defective capillaries.

4 Leaking column-switching valve seal. Exchange the valve seal.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 110

7 Error Information General Error Messages

Leak Sensor Open Error ID: 0083

The leak sensor in the module has failed (open circuit).

The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits. If the current falls outside the lower limit, the error message is generated.

Leak Sensor Short Error ID: 0082

The leak sensor in the module has failed (short circuit).

The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits. If the current increases above the upper limit, the error message is generated.

Probable cause Suggested actions

1 Leak sensor not connected to the power switch board.

Please contact your Agilent service representative.

2 Defective leak sensor. Please contact your Agilent service representative.

3 Leak sensor incorrectly routed, being

pinched by a metal component.

Please contact your Agilent service representative.

4 Power switch assembly defective. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defective leak sensor. Please contact your Agilent service representative.

2 Leak sensor incorrectly routed, being pinched by a metal component.

Please contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 111

7 Error Information General Error Messages

Compensation Sensor Open Error ID: 0081

The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit).

The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor increases above the upper limit, the error message is generated.

Compensation Sensor Short Error ID: 0080

The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit).

The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor falls below the lower limit, the error message is generated.

Probable cause Suggested actions

1 Loose connection between the power switch board and the mainboard.

Please contact your Agilent service representative.

2 Defective power switch assembly. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defective power switch assembly. Please contact your Agilent service representative.

2 Loose connection between the power switch board and the mainboard.

Please contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 112

7 Error Information MCT Error Messages

MCT Error Messages

These errors are module specific.

VALVE INIT FAILED Error ID: 32030

During initialization, the valve actuator turns until the encoder reads the reference index position. An error is generated, if the reference index cannot be found within a given time.

Probable cause Suggested actions

1 Defect in cable connection of valve drive

control.

Please contact your Agilent service representative.

2 Defect in cable connection of valve actuator

encoder reader.

Please contact your Agilent service representative.

3 Defect of valve drive or valve actuator encoder reader.

Please contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 113

7 Error Information MCT Error Messages

VALVE FAILED Error ID: 32031

Switching of the valve failed.

VALVE TAG VIOLATION Error ID: 32032

Reading the valve tag failed.

Probable cause Suggested actions

1 Mechanical problems. Friction too high or blockages on the valve drives motor or on the valve head.

Check valve head for correct installation.

Try to identify the source of trouble by installing a different valve head if possible.

Contact your Agilent service representative.

2 Defective sensor on the valve drive motor Check valve head for correct installation.

Try to identify the source of trouble (valve head or drive) by installing a different valve head if possible.

Contact your Agilent service representative.

Probable cause Suggested actions

1 A valve head has been exchanged

(hot-plugged) while the valve drive was still powered on.

For changing the valve head follow the instructions Replace Valve Heads. It is important to have the MCT switched off for at least 10 s after a new valve head has been installed.

Contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 114

7 Error Information MCT Error Messages

WRONG VALVE Error ID: 32130

Valve not supported in this module.

Probable cause Suggested actions

1 A valve head has been installed which is not supported by G7116A.

Only valve heads up to 800 bar or with up to four positions can be used in G7116A. Exchange the valve head with a suitable one. For changing the valve head follow the instructions Replace Valve Heads.

NOTE For G7116B:

G4232D (5067-4240: 2-position/10-port Valve 1300 bar)

G4231C (5067-4241: 2-position/6-port Valve 1300 bar)

G4234A (5067-4284: 6-position/14-port Valve 800 bar)

G4234C (5067-4273: 6-position/14-port Valve 1300 bar)

G4239C (5067-4233: 8-position/18-port Valve 1300 bar)

5067-4237: 8-position/9-port Valve 1300 bar

For G7116B Bio:

G5641A (5067-6682: 2-position/10-port Valve 1300 bar, bio)

For G7116A:

G4232C (5067-4283: 2-position/10-port Valve 800 bar)

G4231A (5067-4282: 2-position/6-port Valve 800 bar)

G4237A (5067-4279: 4-position/10-port Valve 800 bar)

For G7116A Bio-inert:

G5632A (5067-4132: 2-position/10-port Valve 600 bar, bio-inert)

G5631A (5067-4148: 2-position/6-port Valve 600 bar, bio-inert)

G5639A (5067-4134: 4-position/10-port Valve 600 bar, bio-inert)

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 115

7 Error Information MCT Error Messages

WAIT TIMEOUT Error ID: 32044 (left), 32045 (right)

Wait operation for temperature timed out.

HEATEX OVERTEMP Error ID: 32080 (left), 32081 (right)

The temperature of the Peltier heat sink has exceeded the maximum limit.

UHX SENSOR ERROR Error ID: 32090 (left), 32091 (right)

Upper heat exchanger sensor has an error.

Probable cause Suggested actions

1 Defective heater. Contact your Agilent service representative.

2 Defective mainboard. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defective heater assembly. Please contact your Agilent service representative.

2 Defective mainboard. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defective sensor. Please contact your Agilent service representative.

2 Defective cable. Please contact your Agilent service representative.

3 Defective mainboard. Please contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 116

7 Error Information MCT Error Messages

LHX SENSOR ERROR Error ID: 32092 (left), 32093 (right)

Lower heat exchanger sensor has an error.

LHS SENSOR ERROR Error ID: 32094 (left), 32095 (right)

Heat sink sensor has an error.

Probable cause Suggested actions

1 Defective sensor. Please contact your Agilent service representative.

2 Defective cable. Please contact your Agilent service representative.

3 Defective mainboard. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defective sensor. Please contact your Agilent service representative.

2 Defective cable. Please contact your Agilent service representative.

3 Defective mainboard. Please contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 117

7 Error Information MCT Error Messages

PELTIER OVERCURRENT Error ID: 32098 (left), 32099 (right)

LEFT PELTIER ERROR Error ID: 32096

Left Peltier hardware is not operational.

RIGHT PELTIER ERROR Error ID: 32097

Right Peltier hardware is not operational.

Probable cause Suggested actions

1 Defective sensor. Please contact your Agilent service representative.

2 Defective mainboard. Please contact your Agilent service representative.

3 Defective cable. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defect heater element. Please contact your Agilent service representative.

2 Defective mainboard. Please contact your Agilent service representative.

Probable cause Suggested actions

1 Defect heater element. Please contact your Agilent service representative.

2 Defective mainboard. Please contact your Agilent service representative.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 118

8 Test Functions and Calibrations

Tests and Calibrations in Agilent Lab Advisor 120

Thermostat Test 121

System Pressure Test 122

Sensors Offset Calibration 123

This chapter describes the module's built-in test functions.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 119

8 Test Functions and Calibrations Tests and Calibrations in Agilent Lab Advisor

Tests and Calibrations in Agilent Lab Advisor

Use the tests and diagnostic features provided in the Agilent Lab Advisor software to check if your module is working correctly.

For further details, refer to the Agilent Lab Advisor software help files.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 120

8 Test Functions and Calibrations Thermostat Test

Thermostat Test

Thermostat Test Description

The Thermostat Test is used to evaluate the cooling and heating performance of the two Peltier elements.

The instrument starts the test when the heater temperature is within 5 C of the current measured ambient temperature. After the start, the instrument is cooling down to 4 C below the starting temperature. The cool-down time is used to determine the cooling performance. In the next step, the heating performance is calculated by heating up to the test starting temperature.

Thermostat Test Result

A typical Thermostat Test profile is shown below.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 121

8 Test Functions and Calibrations System Pressure Test

System Pressure Test

For running a System Pressure Test, please refer to the corresponding pump manual. The System Pressure Test may be used for testing the tightness of a valve installed in the MCT.

CAUTION Wrong use of System Pressure Test may damage components.

Do not use higher test max. pressure settings as the lowest rated pressure component in the tests flow path.

NOTE Use appropriate blank nuts to plug ports on the column selection valves:

Blank Nut SL (5067-6127) for standard ports on stainless steel valves,

M4 Blank nut (5067-6141) for M4 ports on stainless steel valves,

Blank nut long 10-32, PEEK (5043-0277) for bio-inert and bio valves.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 122

8 Test Functions and Calibrations Sensors Offset Calibration

Sensors Offset Calibration

The Sensors Offset Calibration tries to find the offset between the two sensors built into the heat exchanger. The idea is to bring both sides to ambient temperature and minimize temperature difference between the heat sink and heat exchanger. This is to ensure that no temperature gradient inside the heat exchanger is present and both temperature sensors are exposed to the exact same temperature.

Because the lower sensor responds much faster to temperature changes by the Peltier, it is also important, that the heat sink is at the same temperature as the heat exchanger. Otherwise, a temperature flow from or to the heat exchanger would generate differences in temperature measured by each sensor.

Required:

After replacement of heater assembly.

After replacement of mainboard.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 123

9 Maintenance

Introduction to Maintenance 125

Cautions and Warnings 127

Overview of Maintenance 129

Cleaning the Module 130

Correcting Leaks 131

Maintain the Column Switching Valve 132

Replace Valve Heads 135

Prepare the MCT for Transportation 139

Replace the Module Firmware 140

This chapter describes the maintenance of the MCT.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 124

9 Maintenance Introduction to Maintenance

Introduction to Maintenance

The module is designed for easy maintenance. The most frequent maintenance such as maintaining valve heads (if optional valve drive is installed) or replacing heat exchangers can be done from the front with the module in place in the system stack.

Figure 18 Overview of maintenance parts (G7116B)

Valve

Door

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 125

9 Maintenance Introduction to Maintenance

Figure 19 Overview of maintenance parts (G7116A)

Valve

Door

NOTE There are no serviceable parts inside.

Do not open the module.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 126

9 Maintenance Cautions and Warnings

Cautions and Warnings

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

The volume of substances should be reduced to the minimum required for the analysis.

Do not operate the instrument in an explosive atmosphere.

WARNING Hot heat exchangers

The column compartment has two heat exchanger assemblies that might be hot.

Allow them to cool down before starting repairs.

WARNING Electrical shock

Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened.

Do not remove the cover of the module.

Only certified persons are authorized to carry out repairs inside the module.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 127

9 Maintenance Cautions and Warnings

WARNING The module is partially energized when switched off, as long as the power cord is plugged in.

Repair work at the module can lead to personal injuries, e.g. electrical shock, when the cover is opened and the module is connected to power.

Always unplug the power cable before opening the cover.

Do not connect the power cable to the instrument while the covers are removed.

WARNING Personal injury or damage to the product

Agilent is not responsible for any damages caused, in whole or in part, by improper use of the products, unauthorized alterations, adjustments or modifications to the products, failure to comply with procedures in Agilent product user guides, or use of the products in violation of applicable laws, rules or regulations.

Use your Agilent products only in the manner described in the Agilent product user guides.

CAUTION Safety standards for external equipment

If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.

CAUTION Sample degradation and contamination of the instrument

Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination.

For bio applications, always use dedicated bio parts, which can be identified by the bio-inert symbol or other markers described in this manual.

Do not mix bio, and non-bio modules or parts in a bio system.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 128

9 Maintenance Overview of Maintenance

Overview of Maintenance

The following pages describe maintenance procedures (simple repairs) that can be done without opening the main cover.

Table 20 Maintenance procedures

Procedure Typical Frequency Notes

Cleaning the Module on page 130 If necessary

Install Heat Exchanger on page 47 When new application requires a change

Installing the capillaries on page 60 When new application requires a change

Correcting Leaks on page 131 If a leak has occurred Check for leaks

Maintain the Column Switching Valve on page 132

If the valve performance shows indication of leakage or wear

Replace Valve Heads on page 135 When repair is not possible or when new application requires a change

Prepare the MCT for Transportation on page 139

If the MCT shall be transported

Replace the Module Firmware on page 140

If necessary

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 129

9 Maintenance Cleaning the Module

Cleaning the Module

To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Avoid using organic solvents for cleaning purposes. They can cause damage to plastic parts.

WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module

Do not use an excessively damp cloth during cleaning.

Drain all solvent lines before opening any connections in the flow path.

NOTE A solution of 70 % isopropanol and 30 % water might be used if the surface of the module needs to be disinfected.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 130

9 Maintenance Correcting Leaks

Correcting Leaks

1 Remove the door.

2 Use a pipette and tissue to dry the leak sensor area.

3 Observe the capillary connections and the column switching valve for leaks and correct, if required.

4 Reinstall the door.

When If a leakage has occurred at the heat exchanger or at the capillary connections or at the column switching valve.

Tools required Description

Tissue

Pipette

Wrench, 1/4 5/16 inch (for capillary connections)

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 131

9 Maintenance Maintain the Column Switching Valve

Maintain the Column Switching Valve

For bio-inert modules use bio-inert parts only!

For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.

When If valve leaks.

Tools required Description

Hexagonal key, 9/64 inch

Hexagonal key, 3/32 inch

Wrench, 1/4 inch

Hexagonal driver SW-6.35 slitted

Hexagonal driver SW-4 slitted

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 132

9 Maintenance Maintain the Column Switching Valve

1 Remove capillaries from ports.

2 Loosen each fixing stator screw two turns at a time. Remove the bolts from the head.

3 Remove the stator head (and stator face if applicable).

4 Remove the stator ring.

5 Remove the rotor seal (and isolation seal if damaged or contaminated).

6 Install the new isolation seal (if required). Ensure the metal spring inside the ring faces towards the valve body.

7 Install the new rotor seal.

8 Replace the stator ring. Ensure the stator ring is flush with the valve body.

9 Place the new (if required) stator face in place on the stator head. Reinstall the stator head.

10 Insert the stator screws in the stator head. Tighten the screws alternately two turns at a time until the stator head is secure.

11 Reconnect the pump capillaries to the valve ports.

12 Perform the System Pressure Test to ensure the valve is leak tight.

CAUTION Wrong use of the System Pressure Test may damage valve.

Always select an appropriate pressure limit for the test. Do not exceed the maximum pressure of pressure sensitive components, for example, set the Maximum Pressure to 800 bar, if an 800 bar Quick Change Valve Head is installed.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 133

9 Maintenance Maintain the Column Switching Valve

Figure 20 Valve Head Parts (example)

1

2

3

4

5

6

7

1 Stator screws

2 Stator head assembly

3 Stator ring screws (not available)

4 Stator ring (available for service only)

5 Rotor seal

6 Bearing ring

7 Spanner nut (available for service only)

NOTE Figure 20 on page 134 illustrates replacement parts for the valve heads, with the 6-column selector valve as an example. The valves can vary in their appearance and do not necessarily include all of the illustrated parts. Neither, every spare part is available for each flavor of the valve.

Use Valve Options Overview (G7116B) on page 146 and Valve Options Overview (G7116A) on page 148 for identification of the required part numbers.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 134

9 Maintenance Replace Valve Heads

Replace Valve Heads

Several optional valve heads are available, which can be installed and exchanged easily.

For details, see Valve Options Overview (G7116B) on page 146 and Valve Options Overview (G7116A) on page 148.

Parts required Description

Agilent Quick Change Valve Head.

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

Be sure that no solvent can drop out of the solvent connections when removing them from your valve head.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

CAUTION Valve Damage

Using a low pressure valve on the high pressure side can damage the valve.

When using multiple column compartments as part of a method development solution, make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector.

NOTE For details, please refer to the InfinityLab LC Method Development Solutions User Guide.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 135

9 Maintenance Replace Valve Heads

CAUTION Column Damage or Bias Measurement Results

Switching the valve to a wrong position can damage the column or bias measurement results.

Fit the lobe to the groove to make sure the valve is switched to the correct position.

CAUTION The valve actuator contains sensitive optical parts, which need to be protected from dust and other pollution. Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results.

Always install a valve head for operation and storage. For protecting the actuator, a dummy valve head (part of Transportation Lock Kit (G1316-67001 )) can be used instead of a functional valve. Do not touch parts inside the actuator.

NOTE The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module. Valve properties will not be updated, if the valve head is replaced while the module is on. Selection of valve port positions can fail, if the instrument does not know the properties of the installed valve.

NOTE To have the valve correctly recognized by the Agilent Infinity Valve Drive you must have the valve drive powered off for at least 10 seconds.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 136

9 Maintenance Replace Valve Heads

1 Switch off the module. 2 Push the valve head to bring it to its outer position.

3 Remove all capillary connections from the valve head. 4 Unscrew the nut and remove the valve head.

5 Put the new valve head onto the valve drive such that the lobe fits to the groove (see also Installing Valve Heads on page 54).

6 Fasten the valve head onto the valve drive using the union nut (see also Installing Valve Heads on page 54).

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9 Maintenance Replace Valve Heads

7 Install all required capillary connections to the valve. 8 Push the valve head until it snaps in and stays in the rear position.

9 Switch on the module.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 138

9 Maintenance Prepare the MCT for Transportation

Prepare the MCT for Transportation

1 Remove the valve head as described in Replace Valve Heads on page 135.

2 Replace the valve head by the transportation valve head. Bring the transportation valve head to the outer position.

3 Fix the Transportation Lock to the MCT.

When If an MCT including the Valve Drive Option shall be transported

NOTE The module has been shipped with transportation locks, which must be used for transportation protection.

CAUTION Damage to Internal Parts

Mechanical shocks for example when being transported by car or shipped by post.

Install a lock (Transportation Lock Kit (G1316-67001 )).

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 139

9 Maintenance Replace the Module Firmware

Replace the Module Firmware

To upgrade/downgrade the modules firmware carry out the following steps:

1 Download the required module firmware, the latest FW Update Tool and the documentation from the Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761

2 For loading the firmware into the module follow the instructions in the documentation.

Module Specific Information

Module is a hosted module and always needs to be connected to a host with matching firmware of same revision.

When The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same (validated) revision.

The installation of older firmware might be necessary to keep all systems on the same (validated) revision or if a new module with newer firmware is added to a system or if third party control software requires a special version.

Tools required Description

Agilent Lab Advisor software

Parts required # Description

1 Firmware, tools and documentation from Agilent web site

Preparations Read update documentation provided with the Firmware Update Tool.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 140

10 Parts and Materials for Maintenance

Plastic Parts 142

Leak Parts 143

Valve Options and Parts 144 Valve Options Overview (for G7116B) 146 Valve Options Overview (for G7116A) 148

Column ID Parts 150

Accessories and Consumables 151 Accessories and Consumables (for G7116B) 151 Accessories and Consumables (for G7116A) 153 Additional Heater Devices 155 InfinityLab Quick Connect and Quick Turn Fittings 157

This chapter provides information on parts for maintenance.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 141

10 Parts and Materials for Maintenance Plastic Parts

Plastic Parts

Figure 21 Plastic parts

1

2

3

4

5

6

7

8

Item p/n Description

1 G7116-60004A Door Kit Infinity II MCT (for G7116B)

OR 1 G7116-60001A Door Kit Infinity II MCT (for G7116A)

G7116-68713A Cabinet Kit Infinity II MCT includes parts 2 7:

2 G7116-40100A Base Cover Infinity II MCT

3 G7116-40103A Side Cover Left Insert Infinity II MCT

4 Side Cover Left Infinity II MCT (available only as a part of the kit)

5 Top Cover Kit Infinity II (available only as a part of the kit)

6 Side Cover Right Infinity II MCT (available only as a part of the kit)

7 G7116-40106A Side Cover Right Insert Infinity II MCT

8 G7116-67004A MCT Hinge Base Assembly Kit (repair kit for MCT door hinge base)

G7116-60003A Transportation Foam Assembly (not shown)

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10 Parts and Materials for Maintenance Leak Parts

Leak Parts

Figure 22 Plastic parts

1

2

Item p/n Description

1 G7116-40043A Leak Adapter Top MCT

2 G7116-40013A Leak Adapter Hitch MCT

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10 Parts and Materials for Maintenance Valve Options and Parts

Valve Options and Parts

MCT:

External Valve Drive:

p/n Description

G1353DA Valve drive upgrade kit for MCT

p/n Description

G1170AA External Valve Drive

5067-6138A Valve Holder Kit Right-IF-II-G

5067-6139A Valve Holder Kit Left-IF-II-G

G1170-68705A Accessory Kit with Clamp for mounting on 1290 Infinity II module

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10 Parts and Materials for Maintenance Valve Options and Parts

Valve Head Parts

Figure 23 Valve Head Parts (example)

NOTE The figure below illustrates replacement parts for the valve heads, with the 12-position/13-port Selector valve as an example. The valves can vary in their appearance and do not necessarily include all of the illustrated parts. Neither, every spare part is available for each flavor of the valve.

Use the tables (Valve Options Overview (G7116B) on page 146 and Valve Options Overview (G7116A) on page 148) for identification of the required part numbers.

1

2

3

4

5

6

7

1 Stator screws

2 Stator head assembly

3 Stator ring screws (not available)

4 Stator ring (available for service only)

5 Rotor seal

6 Bearing ring

7 Spanner nut (available for service only)

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10 Parts and Materials for Maintenance Valve Options and Parts

Valve Options Overview (for G7116B)

Valve Options Overview (G7116B)

Table 21 Replacement parts standard valve heads for G7116B

Valve Head Rotor Seal Stator Head Stator Screws Stator Ring

5067-4233 8-Position/18-Port Valve 1300 bar

5068-0200 (PEEK)

5068-0199 5068-0089 n.a.

5067-4241 2-Position/6-Port Valve 1300 bar

5068-0207 (PEEK)

5068-0006 1535-4857 5068-0120

5067-4240 2-Position/10-Port Valve 1300 bar

5068-0205 (PEEK)

5068-0011 5068-0019 n.a.

5067-4273 6-Position/14-Port Valve 1300 bar

5068-0242 (PEEK)

5068-0241 5068-0089 n.a.

5067-4284 6-Position/14-Port Valve 800 bar

5068-0298 (PEEK)

5068-0241 5068-0089 n.a.

5067-6682 2-Position/10-Port Valve Bio 1300 bar

5068-0205 (PEEK)

5068-0286 5068-0019 n.a.

5067-4237 8-Position/9-Port Valve 1300 bar

5068-0202 (PEEK)

5068-0001 1535-4857 5068-0120

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10 Parts and Materials for Maintenance Valve Options and Parts

Obsolete Valve Heads

The following 1200 bar valve heads are no longer orderable:

Table 22 Replacement parts obsolete valve heads for G7116B

Valve Head Rotor Seal Stator Head Stator Screws Stator Ring

5067-4121 8-Position/9-Port Valve 1200 bar

5068-0002 (Vespel)

5068-0001 1535-4857 5068-0127

5067-4117 2-Position/6-Port Valve 1200 bar

5068-0008 (Vespel)

5068-0006 1535-4857 5068-0127

5067-4118 2-Position/10-Port Valve 1200 bar

5068-0012 (Vespel)

5068-0011 5068-0019 n.a.

5067-4142 6-Position/14-Port Valve 1200 bar

5068-0067 (Vespel)

5068-0077 5068-0089 n.a.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 147

10 Parts and Materials for Maintenance Valve Options and Parts

Valve Options Overview (for G7116A)

Valve Options Overview (G7116A)

Table 23 Replacement parts standard valve heads for G7116A

Valve Head Rotor Seal Stator Head Stator Screws Stator Ring Stator face Other

5067-4279 4-Position/10-Port Valve 800 bar

5068-0264 (PEEK)

5068-0263 5068-0019 n.a. n.a. Bearing ring: 1535-4045

5067-4282 2-Position/6-Port Valve 800 bar

0101-1409 (PEEK)

0101-1417 1535-4857 5068-0120 n.a. Bearing ring: 1535-4045

5067-4148 2-Position/6-Port Bio-Inert Valve 600 bar

0101-1409 (PEEK)

5068-0060 5068-0020 (10/pack)

n.a. 0100-1851 (ceramic)

Bearing ring: 1535-4045

5067-4132 2-Position/10-Port Bio-Inert Valve 600 bar

5068-0041 (PEEK)

5068-0040 5068-0059 n.a. 5068-0095 Bearing ring: 1535-4045

5067-4283 2-Position/10-Port Valve 800 bar

0101-1415 (PEEK)

5068-0165 5068-0019 n.a. n.a. Bearing ring: 1535-4045

5067-4134 4-Position/10-Port Bio-Inert Valve 600 bar

5068-0045 (PEEK)

5068-0044 5068-0059 n.a. 5068-0093 Bearing ring: 1535-4045

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 148

10 Parts and Materials for Maintenance Valve Options and Parts

Obsolete Valve Heads

The following 600 bar valve heads are no longer orderable:

Table 24 Replacement parts obsolete valve heads for G7116A

Valve Head Rotor Seal Stator Head Stator Screws Stator Ring

5067-4287 4-Position/10-Port Valve 600 bar

5068-0264 (PEEK)

5068-0263 5068-0019 n.a.

5067-4137 2-Position/6-Port Valve 600 bar

0101-1409 (PEEK)

0101-1417 5068-0018 5068-0127

5067-4145 2-Position/10-Port Valve 600 bar

0101-1415 (PEEK)

5068-0165 5068-0019 n.a.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 149

10 Parts and Materials for Maintenance Column ID Parts

Column ID Parts

Column ID Upgrade Kit (for G7116B)

Column ID Upgrade Kit (for G7116A)

Column ID Parts (for G7116B)

Column ID Parts (for G7116A)

p/n Description

G4750BA Column ID upgrade kit for G7116B

p/n Description

G4750AA Column ID upgrade kit for G7116A

p/n Description

G7116-81001A Column ID flex-board connection, right

G7116-81002A Column ID flex-board connection, left

5067-5915A Column ID Kit Left

5067-5916A Column ID Kit Right

5067-5917A InfinityLab Column Identification Tag

p/n Description

G7116-81002A Column ID flex-board connection, left

5067-5915A Column ID Kit Left

5067-5917A InfinityLab Column Identification Tag

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10 Parts and Materials for Maintenance Accessories and Consumables

Accessories and Consumables

Accessories and Consumables (for G7116B)

G7116-68705 Accessory Kit (for G7116B)

The Accessory Kit (for G7116B) contains accessories and tools needed for the installation and maintenance.

p/n Description

5181-1516A CAN cable, Agilent module to module, 0.5 m

5063-6527A Tubing, Silicon Rubber, 1.2 m, ID/OD 6/9 mm

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket

5067-5966A InfinityLab Quick Turn Fitting

5067-5957A InfinityLab Quick Connect Assy ST 0.12 mm x 105 mm

G7116-60015A Quick Connect Heat Exchanger Standard

G7116-68003A Column Holder Lamella, 2/pk (delivered as a part of G7116-60015)

5043-0915A Fitting mounting tool

G7116-60006A Divider Assembly MCT

5022-2184A Union, stand LC flow, no fitting

Double Drain Connector

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10 Parts and Materials for Maintenance Accessories and Consumables

G7116-68707 Accessory Kit Bio (for G7116B)

Available Consumables (for G7116B)

p/n Description

5181-1516A CAN cable, Agilent module to module, 0.5 m

5063-6527A Tubing, Silicon Rubber, 1.2 m, ID/OD 6/9 mm

5500-1596A Quick Turn Capillary MP35N 0.12 mm x 280 mm

5067-5966A InfinityLab Quick Turn Fitting

G7116-60071A Quick Connect Bio Heat Exchanger Standard Flow

G7116-68003A Column Holder Lamella, 2/pk (delivered as a part of G7116-60071)

5043-0915A Fitting mounting tool

G7116-60006A Divider Assembly MCT

5023-2625A Union MP35N

Double Drain Connector

5500-1578A Quick Connect Capillary MP35N 0.12 mm x 105 mm

5067-5965A InfinityLab Quick Connect LC fitting

5500-1598A Quick Turn Capillary MP35N 0.12 mm x 500 mm

p/n Description

G7116-68003A Column Holder Lamella, 2/pk

G7116-68004A Column Holder Clamp, 2/pk

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket Capillary from column outlet to DAD, no fittings.

G7116-60006A Divider Assembly MCT For separating different temperature zones between left and right heater elements.

5067-5917A InfinityLab Column Identification Tag Blank column ID tag (column ID tag reader kit is required)

G7116-60013A InfinityLab Thermal Equilibration Device

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10 Parts and Materials for Maintenance Accessories and Consumables

Accessories and Consumables (for G7116A)

G7116-68755 Accessory Kit (for G7116A)

The Accessory Kit (for G7116A) contains accessories and tools needed for the installation and maintenance.

p/n Description

5181-1516A CAN cable, Agilent module to module, 0.5 m

5063-6527A Tubing, Silicon Rubber, 1.2 m, ID/OD 6/9 mm

0100-1516A Finger-tight fitting PEEK, 2/pk

5500-1193A InfinityLab Quick Turn Capillary ST 0.17 mm x 105 mm, long socket

G7167-68703A Fitting Intermediate Kit

G7116-60051A Quick Connect Heat Exchanger Large ID

G7116-68003A Column Holder Lamella, 2/pk (delivered as a part of G7116-60051)

5043-0915A Fitting mounting tool

5067-5966A InfinityLab Quick Turn Fitting

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket

5022-2184A Union, stand LC flow, no fitting

Double Drain Connector

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10 Parts and Materials for Maintenance Accessories and Consumables

G7116-68715 Bio-inert Accessory Kit (for G7116A)

Available Consumables (for G7116A)

p/n Description

5181-1516A CAN cable, Agilent module to module, 0.5 m

5067-4780A Bio Capillary 0.17 mm x 300 mm, 1.6 mm OD socket

G7116-68003A Column Holder Lamella, 2/pk

0100-1516A Finger-tight fitting PEEK, 2/pk

G7116-60041A Quick Connect Heat Exchanger Bio-inert

5043-0915A Fitting mounting tool

5063-6527A Tubing, Silicon Rubber, 1.2 m, ID/OD 6/9 mm

5067-5403A UHP fitting

5067-4741A ZDV union (Bio-inert)

p/n Description

G7116-68003A Column Holder Lamella, 2/pk

G7116-68004A Column Holder Clamp, 2/pk

G7116-60006A Divider Assembly MCT For separating different temperature zones between left and right heater elements.

5067-5917A InfinityLab Column Identification Tag Blank column ID tag (column ID tag reader kit is required)

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket Capillary from column outlet to DAD, no fittings.

G7116-60013A InfinityLab Thermal Equilibration Device

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 154

10 Parts and Materials for Maintenance Accessories and Consumables

Additional Heater Devices

Quick Connect Heat Exchangers with 0.12 mm and 0.075 mm i.d. capillary are suitable for G7116B. Quick Connect Heat Exchangers with 0.17 mm i.d. are suitable for G7116A.

Table 25 Heat Exchanger Overview

Flow rate 0.075 mm i.d. capillary 0.12 mm i.d. capillary 0.17 mm i.d. capillary

< 2 mL/min Ultra-low Dispersion G7116-60021 (Internal volume: 1.0 L)

Standard Flow G7116-60015 (Internal volume: 1.6 L)

Large ID G7116-60051 (Internal volume: 3.0 L)

> 2 mL/min High Flow G7116-60031 (Internal volume: 3.0 L)

Large ID High Flow G7116-60061 (Internal volume: 6.0 L)

Bio, all flow rates

Bio Ultra-low Dispersion G7116-60091 (Internal volume: 1.0 L)

Bio Standard Flow G7116-60071 (Internal volume: 1.6 L) Bio High Flow G7116-60081 (Internal volume: 3.0 L)

Bio-inert G7116-60041 (Internal volume: 9.0 L)

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10 Parts and Materials for Maintenance Accessories and Consumables

Table 26 InfinityLab Quick Connect Heat Exchangers

Item Description

Old design Quick Connect Heat Exchanger: twist lock clips of the Quick Connect Heat Exchanger are used to secure the Quick Connect Heat Exchanger in the heater block of the MCT.

New design Quick Connect Heat Exchanger: column holders (lamella type G7116-68003 or clamp type G7116-68004) are used to secure the Quick Connect Heat Exchanger in the heater block of the MCT. G7116-68003 lamella type column holders are delivered as a part of the Quick Connect Heat Exchanger and do not need to be ordered separately.

Design has been changed for all Quick Connect Heat Exchangers except G7116-60041, Quick Connect Heat Exchanger Bio-inert.

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10 Parts and Materials for Maintenance Accessories and Consumables

InfinityLab Quick Connect and Quick Turn Fittings

InfinityLab Quick Connect Fittings

Figure 24 InfinityLab Quick Connect Fitting

p/n Description

5067-5965A InfinityLab Quick Connect LC fitting (fitting without preinstalled capillary)

5043-0924A Front Ferrule for Quick Connect/Turn Fitting

5067-5961A InfinityLab Quick Connect Assy ST 0.075 mm x 105 mm

5067-6163A InfinityLab Quick Connect Assy ST 0.075 mm x 150 mm

5067-6164A InfinityLab Quick Connect Assy ST 0.075 mm x 220 mm

5067-6165A InfinityLab Quick Connect Assy ST 0.075 mm x 280 mm

5067-5957A InfinityLab Quick Connect Assy ST 0.12 mm x 105 mm

5067-5958A InfinityLab Quick Connect Assy ST 0.12 mm x 150 mm

5067-5959A InfinityLab Quick Connect Assy ST 0.12 mm x 220 mm

5067-5960A InfinityLab Quick Connect Assy ST 0.12 mm x 280 mm

5067-6166A InfinityLab Quick Connect Assy ST 0.17 mm x 105 mm

5067-6167A InfinityLab Quick Connect Assy ST 0.17 mm x 150 mm

5067-6168A InfinityLab Quick Connect Assy ST 0.17 mm x 220 mm

5067-6169A InfinityLab Quick Connect Assy ST 0.17 mm x 280 mm

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10 Parts and Materials for Maintenance Accessories and Consumables

InfinityLab Quick Connect Fitting Replacement Capillaries

p/n Description

5500-1174A InfinityLab Capillary ST 0.075 mm x 105 mm

5500-1175A InfinityLab Capillary ST 0.075 mm x 150 mm

5500-1176A InfinityLab Capillary ST 0.075 mm x 220 mm

5500-1177A InfinityLab Capillary ST 0.075 mm x 250 mm

5500-1178A InfinityLab Capillary ST 0.075 mm x 280 mm

5500-1173A InfinityLab Capillary ST 0.12 mm x 105 mm

5500-1172A InfinityLab Capillary ST 0.12 mm x 150 mm

5500-1171A InfinityLab Capillary ST 0.12 mm x 220 mm

5500-1170A InfinityLab Capillary ST 0.12 mm x 280 mm

5500-1179A InfinityLab Capillary ST 0.12 mm x 400 mm

5500-1180A InfinityLab Capillary ST 0.12 mm x 500 mm

5500-1181A InfinityLab Capillary ST 0.17 mm x 105 mm

5500-1182A InfinityLab Capillary ST 0.17 mm x 150 mm

5500-1183A InfinityLab Capillary ST 0.17 mm x 220 mm

5500-1230A InfinityLab Capillary ST 0.17 mm x 280 mm

5500-1231A InfinityLab Capillary ST 0.17 mm x 500 mm

5500-1259A InfinityLab Capillary ST 0.25 mm x 150 mm

5500-1260A InfinityLab Capillary ST 0.25 mm x 400 mm

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10 Parts and Materials for Maintenance Accessories and Consumables

InfinityLab Quick Turn Fitting

Figure 25 InfinityLab Quick Turn Fitting

p/n Description

5067-5966A InfinityLab Quick Turn Fitting

5043-0924A Front Ferrule for Quick Connect/Turn Fitting

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10 Parts and Materials for Maintenance Accessories and Consumables

Capillaries for use with the InfinityLab Quick Turn Fitting

p/n Description

5500-1198A Capillary ST 0.075 mm x 105 mm, long socket

5500-1232A Capillary ST 0.075 mm x 150 mm, long socket

5500-1206A Capillary ST 0.075 mm x 250 mm, long socket

5500-1205A Capillary ST 0.075 mm x 500 mm, long socket

5500-1188A Quick Turn Capillary ST 0.12 mm x 105 mm, long socket

5500-1189A Capillary ST 0.12 x 150 mm, long socket

5500-1233A Capillary ST 0.12 mm x 180 mm, long socket

5500-1190A Capillary ST 0.12 mm x 200 mm, long socket

5500-1191A InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket

5500-1192A Capillary ST 0.12 mm x 500 mm, long socket

5500-1193A InfinityLab Quick Turn Capillary ST 0.17 mm x 105 mm, long socket

5500-1194A Capillary ST 0.17 mm x 150 mm, long socket

5500-1234A Capillary ST 0.17 mm x 180 mm, long socket

5500-1195A Capillary ST 0.17 mm x 200 mm, long socket

5500-1196A Capillary ST 0.17 mm x 280 mm, long socket

5500-1235A Capillary ST 0.17 mm x 380 mm, long socket

5500-1236A Capillary ST 0.17 mm x 400 mm, long socket

5500-1197A Capillary ST 0.17 mm x 500 mm, long socket

5500-1237A Capillary 0.17 mm x 700 mm, ns/ns

5500-1262A Capillary 0.25 mm x 150 mm, ns/ns

5500-1263A Capillary ST 0.25 mm x 400 mm, long socket

5500-1200A Quick Turn Capillary ST 0.12 mm x 130 mm SL/M

5500-1288A Capillary ST 0.12 mm x 150 mm, long socket, M4

5500-1290A Capillary ST 0.17 mm x 150 mm, long socket, M4

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10 Parts and Materials for Maintenance Accessories and Consumables

InfinityLab Quick Connect Fitting Replacement Bio-compatible Capillaries

NOTE Bio-compatible MP35N capillaries are color-coded and have an orange stripe in addition to color-coding of capillary internal diameters (0.075 mm - black, 0.12 mm - red, 0.17 mm - green, 0.25 mm - blue).

p/n Description

5500-1474A Quick Connect Capillary MP35N 0.075 mm x 105 mm

5500-1475A Quick Connect Capillary MP35N 0.075 mm x 150 mm

5500-1576A Quick Connect Capillary MP35N 0.075 mm x 220 mm

5500-1577A Quick Connect Capillary MP35N 0.075 mm x 280 mm

5500-1578A Quick Connect Capillary MP35N 0.12 mm x 105 mm

5500-1579A Quick Connect Capillary MP35N 0.12 mm x 150 mm

5500-1580A Quick Connect Capillary MP35N 0.12 mm x 220 mm

5500-1581A Quick Connect Capillary MP35N 0.12 mm x 280 mm

5500-1582A Quick Connect Capillary MP35N 0.12 mm x 400 mm

5500-1583A Quick Connect Capillary MP35N 0.12 mm x 500 mm

5500-1584A Quick Connect Capillary MP35N 0.17 mm x 105 mm

5500-1585A Quick Connect Capillary MP35N 0.17 mm x 150 mm

5500-1586A Quick Connect Capillary MP35N 0.17 mm x 220 mm

5500-1587A Quick Connect Capillary MP35N 0.17 mm x 280 mm

5500-1588A Quick Connect Capillary MP35N 0.17 mm x 500 mm

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10 Parts and Materials for Maintenance Accessories and Consumables

InfinityLab Quick Turn Fitting Replacement Bio-compatible Capillaries

NOTE Bio-compatible MP35N capillaries are color-coded and have an orange stripe in addition to color-coding of capillary internal diameters (0.075 mm - black, 0.12 mm - red, 0.17 mm - green, 0.25 mm - blue).

p/n Description

5500-1589A Quick Turn Capillary MP35N 0.075 mm x 105 mm

5500-1590A Quick Turn Capillary MP35N 0.075 mm x 150 mm

5500-1591A Quick Turn Capillary MP35N 0.075 mm x 250 mm

5500-1592A Quick Turn Capillary MP35N 0.075 mm x 500 mm

5500-1593A Quick Turn Capillary MP35N 0.12 mm x 105 mm

5500-1594A Quick Turn Capillary MP35N 0.12 mm x 150 mm

5500-1595A Quick Turn Capillary MP35N 0.12 mm x 200 mm

5500-1596A Quick Turn Capillary MP35N 0.12 mm x 280 mm

5500-1597A Quick Turn Capillary MP35N 0.12 mm x 400 mm

5500-1598A Quick Turn Capillary MP35N 0.12 mm x 500 mm

5500-1599A Quick Turn Capillary MP35N 0.17 mm x 105 mm

5500-1600A Quick Turn Capillary MP35N 0.17 mm x 150 mm

5500-1601A Quick Turn Capillary MP35N 0.17 mm x 200 mm

5500-1602A Quick Turn Capillary MP35N 0.17 mm x 280 mm

5500-1603A Quick Turn Capillary MP35N 0.17 mm x 400 mm

5500-1604A Quick Turn Capillary MP35N 0.17 mm x 500 mm

5500-1605A Quick Turn Capillary MP35N 0.17 mm x 700 mm

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 162

11 Identifying Cables

Cable Overview 164

Analog Cables 166

Remote Cables 168

CAN/LAN Cables 172

RS-232 Cables 173

USB 174

This chapter provides information on cables used with the Agilent Infinity and Infinity II Series modules.

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11 Identifying Cables Cable Overview

Cable Overview

Analog cables

Remote cables

CAN cables

NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

p/n Description

35900-60750A Agilent 35900A A/D converter

01046-60105A Analog cable (BNC to general purpose, spade lugs)

p/n Description

5188-8029A ERI to general purpose

5188-8044A Remote Cable ERI ERI

5188-8045A Remote Cable APG ERI

5188-8059A ERI-Extension-Cable 1.2 m

5061-3378A Remote Cable to 35900 A/D converter

01046-60201A Agilent module to general purpose

5188-8057A Fraction Collection ERI remote Y-cable

p/n Description

5181-1516A CAN cable, Agilent module to module, 0.5 m

5181-1519A CAN cable, Agilent module to module, 1 m

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 164

11 Identifying Cables Cable Overview

LAN cables

RS-232 cables (not for

FUSION board)

USB cables

p/n Description

5023-0203A Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202A Twisted pair network cable, shielded, 7 m (for point to point connection)

p/n Description

RS232-61601A RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561A RS-232 cable, 8 m

p/n Description

5188-8050A USB A M-USB Mini B 3 m (PC-Module)

5188-8049A USB A F-USB Mini B M OTG (Module to Flash Drive)

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11 Identifying Cables Analog Cables

Analog Cables

One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made.

Agilent Module to 35900 A/D converters

p/n 35900-60750 35900 Pin Agilent module

Signal Name

1 Not connected

2 Shield Analog -

3 Center Analog +

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11 Identifying Cables Analog Cables

Agilent Module to BNC Connector

Agilent Module to General Purpose

p/n 8120-1840 Pin BNC Pin Agilent module

Signal Name

Shield Shield Analog -

Center Center Analog +

p/n 01046-60105 Pin Pin Agilent module

Signal Name

1 Not connected

2 Black Analog -

3 Red Analog +

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 167

11 Identifying Cables Remote Cables

Remote Cables

ERI (Enhanced Remote Interface)

5188-8029 ERI to general purpose (D-Sub 15 pin male - open end)

5188-8044 ERI to ERI (D_Sub 15 pin male - male)

5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female)

p/n 5188-8029 pin Color code Enhanced Remote

Classic Remote

Active (TTL)

1 white IO1 START REQUEST

Low

2 brown IO2 STOP Low

3 green IO3 READY High

4 yellow IO4 POWER ON High

5 grey IO5 NOT USED

6 pink IO6 SHUT DOWN Low

7 blue IO7 START Low

8 red IO8 PREPARE Low

9 black 1wire DATA

10 violet DGND

11 grey-pink +5V ERI out

12 red-blue PGND

13 white-green PGND

14 brown-green +24V ERI out

15 white-yellow +24V ERI out

NC yellow-brown

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11 Identifying Cables Remote Cables

5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG))

p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL)

10 GND 1

1 Start Request 9 Low

2 Stop 8 Low

3 Ready 7 High

5 Power on 6 High

4 Future 5

6 Shut Down 4 Low

7 Start 3 Low

8 Prepare 2 Low

Ground Cable Shielding NC

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11 Identifying Cables Remote Cables

5188-8057 ERI to APG and RJ45 (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG), Connector plug Cat5e (RJ45))

One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.

Table 27 5188-8057 ERI to APG and RJ45

p/n 5188-8057 Pin (ERI) Signal Pin (APG) Active (TTL) Pin (RJ45)

10 GND 1 5

1 Start Request

9 High

2 Stop 8 High

3 Ready 7 High

4 Fraction Trigger

5 High 4

5 Power on 6 High

6 Shut Down 4 High

7 Start 3 High

8 Prepare 2 High

Ground Cable Shielding

NC

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11 Identifying Cables Remote Cables

Agilent Module to Agilent 35900 A/D Converters

Agilent Module to General Purpose

p/n 5061-3378 Pin 35900 A/D Pin Agilent module

Signal Name Active (TTL)

1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low

3 - Gray 3 - Gray Start Low

4 - Blue 4 - Blue Shut down Low

5 - Pink 5 - Pink Not connected

6 - Yellow 6 - Yellow Power on High

7 - Red 7 - Red Ready High

8 - Green 8 - Green Stop Low

9 - Black 9 - Black Start request Low

p/n 01046-60201 Wire Color Pin Agilent module

Signal Name Active (TTL)

White 1 Digital ground

Brown 2 Prepare run Low

Gray 3 Start Low

Blue 4 Shut down Low

Pink 5 Not connected

Yellow 6 Power on High

Red 7 Ready High

Green 8 Stop Low

Black 9 Start request Low

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 171

11 Identifying Cables CAN/LAN Cables

CAN/LAN Cables

Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors.

CAN Cables

LAN Cables

p/n Description

5181-1516A CAN cable, Agilent module to module, 0.5 m

5181-1519A CAN cable, Agilent module to module, 1 m

p/n Description

5023-0203A Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202A Twisted pair network cable, shielded, 7 m (for point to point connection)

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 172

11 Identifying Cables RS-232 Cables

RS-232 Cables

p/n Description

RS232-61601A RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561A RS-232 cable, 8 m

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11 Identifying Cables USB

USB

To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket.

p/n Description

5188-8050A USB A M-USB Mini B 3 m (PC-Module)

5188-8049A USB A F-USB Mini B M OTG (Module to Flash Drive)

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 174

12 Hardware Information

Firmware Description 176

Electrical Connections 179 Rear View of the Module 180 Information on Instrument Serial Number 181

Interfaces 182 Overview Interfaces 184 ERI (Enhanced Remote Interface) 187 USB (Universal Serial Bus) 189

2-bit Configuration Switch 190

Instrument Layout 191

Early Maintenance Feedback (EMF) 192

This chapter describes the module in more detail on hardware and electronics.

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12 Hardware Information Firmware Description

Firmware Description

The firmware of the instrument consists of two independent sections:

a non-instrument specific section, called resident system

an instrument specific section, called main system

Resident System

This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules. Its properties are:

the complete communication capabilities (CAN, LAN, USB and RS- 232)

memory management

ability to update the firmware of the 'main system'

Main System

Its properties are:

the complete communication capabilities (CAN, LAN, USB and RS- 232)

memory management

ability to update the firmware of the 'resident system'

In addition the main system comprises the instrument functions that are divided into common functions like

run synchronization through APG/ERI remote,

error handling,

diagnostic functions,

or module specific functions like

internal events such as lamp control, filter movements,

raw data collection and conversion to absorbance.

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12 Hardware Information Firmware Description

Firmware Updates

Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used).

Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761

The file naming conventions are:

PPPP_RVVV_XXX.dlb, where

PPPP is the product number, for example, 1315B for the G1315B DAD,

R the firmware revision, for example, A for G1315B or B for the G1315C DAD,

VVV is the revision number, for example 650 is revision 6.50,

XXX is the build number of the firmware.

For instructions on firmware updates refer to section Replacing Firmware in chapter "Maintenance" or use the documentation provided with the Firmware Update Tools.

Figure 26 Firmware Update Mechanism

NOTE Update of main system can be done in the resident system only. Update of the resident system can be done in the main system only.

Main and resident firmware must be from the same set.

Resident System Main FW update

Main System

Resident FW Update

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12 Hardware Information Firmware Description

The firmware update tools, firmware and documentation are available from the Agilent web.

http://www.agilent.com/en-us/firmwareDownload?whid=69761

NOTE Some modules are limited in downgrading due to their mainboard version or their initial firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx.

Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific control software environments. In this case, the feature set of the target type is used and the feature set of the original one is lost. After re-branding (e.g. from G1314B to G1314C), the original feature set is available again.

All this specific information is described in the documentation provided with the firmware update tools.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 178

12 Hardware Information Electrical Connections

Electrical Connections

The CAN bus is a serial bus with high-speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization.

The ERI/REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shutdown, prepare, and so on.

With the appropriate software, the LAN connector may be used to control the module from a computer through a LAN connection. This connector is activated and can be configured with the configuration switch.

With the appropriate software, the USB connector may be used to control the module from a computer through a USB connection.

The power input socket accepts a line voltage of 100 240 VAC 10 % with a line frequency of 50 or 60 Hz. Maximum power consumption varies by module. There is no voltage selector on your module because the power supply has wide-ranging capability. There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply.

NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 179

12 Hardware Information Electrical Connections

Rear View of the Module

Figure 27 Rear view of the Multicolumn Thermostat G7116A/B

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 180

12 Hardware Information Electrical Connections

Information on Instrument Serial Number

Serial Number Information 1200 Series and 1290 Infinity

The serial number information on the instrument labels provide the following information:

Serial Number Information 1260/1290 Infinity

The serial number information on the instrument labels provide the following information:

CCYWWSSSSS Format

CC country of manufacturing DE = Germany JP = Japan CN = China

YWW year and week of last major manufacturing change, e.g. 820 could be week 20 of 1998 or 2008

SSSSS real serial number

CCXZZ00000 Format

CC Country of manufacturing DE = Germany JP = Japan CN = China

X Alphabetic character A-Z (used by manufacturing)

ZZ Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)

00000 Serial number

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 181

12 Hardware Information Interfaces

Interfaces

The Agilent InfinityLab LC Series modules provide the following interfaces:

Table 28 Agilent InfinityLab LC Series Interfaces

Module CAN USB LAN (on-board)

RS-232 Analog APG (A) / ERI (E)

Special

Pumps

G7104A/C 2 No Yes Yes 1 A

G7110B 2 Yes Yes No No E

G7111A/B, G5654A 2 Yes Yes No No E

G7112B 2 Yes Yes No No E

G7120A, G7132A 2 No Yes Yes 1 A

G7161A/B 2 Yes Yes No No E

Samplers

G7129A/B/C 2 Yes Yes No No E

G7167A/B, G7137A, G5668A, G3167A

2 Yes Yes No No E

G7157A 2 Yes Yes No No E

Detectors

G7114A/B 2 Yes Yes No 1 E

G7115A 2 Yes Yes No 1 E

G7117A/B/C 2 Yes Yes No 1 E

G7121A/B 2 Yes Yes No 1 E

G7162A/B 2 Yes Yes No 1 E

G7165A 2 Yes Yes No 1 E

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 182

12 Hardware Information Interfaces

CAN connectors as interface to other modules

LAN connector as interface to the control software

RS-232C as interface to a computer

USB (Universal Series Bus) as interface to a computer

REMOTE connector as interface to other Agilent products

Analog output connector(s) for signal output

Fraction Collectors

G7158B 2 Yes Yes No No E

G7159B 2 Yes Yes No No E

G7166A 2 No No No No No Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card

G1364E/F, G5664B 2 Yes Yes No No E THERMOSTAT for G1330B

Others

G1170A 2 No No No No No

G7116A/B 2 No No No No No Requires a host module with on-board LAN or with additional G1369C LAN Card.

G7122A No No No Yes No A

G7170B 2 No No No No No Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card

Table 28 Agilent InfinityLab LC Series Interfaces

Module CAN USB LAN (on-board)

RS-232 Analog APG (A) / ERI (E)

Special

NOTE The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via LAN. The inter-module communication is done via CAN.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 183

12 Hardware Information Interfaces

Overview Interfaces

CAN

The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement.

LAN

The modules have either an interface slot for a LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD). This interface allows the control of the module/system via a PC with the appropriate control software. Some modules have neither on-board LAN nor an interface slot for a LAN card (e.g. G1170A Valve Drive or G4227A Flexible Cube). These are hosted modules and require a Host module with firmware B.06.40 or later or with additional G1369C LAN Card.

USB

The USB interface replaces the RS-232 Serial interface in new FUSION generation modules. For details on USB refer to USB (Universal Serial Bus) on page 189.

Analog Signal Output

The analog signal output can be distributed to a recording device. For details refer to the description of the modules mainboard.

NOTE If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN should be connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent detector is part of the system, the LAN interface should be installed in the pump or autosampler.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 184

12 Hardware Information Interfaces

Remote (ERI)

The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on.

It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.

The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique).

To provide maximum safety within a distributed analysis system, one line is dedicated to SHUT DOWN the systems critical parts in case any module detects a serious problem. To detect whether all participating modules are switched on or properly powered, one line is defined to summarize the POWER ON state of all connected modules. Control of analysis is maintained by signal readiness READY for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal levels are defined as:

standard TTL levels (0 V is logic true, + 5.0 V is false),

fan-out is 10,

input load is 2.2 kOhm against + 5.0 V, and

output are open collector type, inputs/outputs (wired- or technique).

NOTE All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with respect to the ground terminal).

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12 Hardware Information Interfaces

Special Interfaces

There is no special interface for this module.

Table 29 ERI signal distribution

Pin Signal Description

1 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler.

2 STOP (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection). Receiver is any module performing run-time controlled activities.

3 READY (H) System is ready for next analysis. Receiver is any sequence controller.

4 POWER ON (H) All modules connected to system are switched on. Receiver is any module relying on operation of others.

5 Not used

6 SHUT DOWN (L) System has serious problem (for example, leak: stops pump). Receiver is any module capable to reduce safety risk.

7 START (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities.

8 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities.

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12 Hardware Information Interfaces

ERI (Enhanced Remote Interface) ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2)

ERI Description

The ERI interface contains eight individual programmable input/output pins. In addition, it provides 24 V power and 5 V power and a serial data line to detect and recognize further add-ons that could be connected to this interface. This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc.

Figure 28 Location of the ERI interface (example shows a G7114A/B VWD)

ERI

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12 Hardware Information Interfaces

IO (Input/Output) Lines

Eight generic bi-directional channels (input or output).

Same as the APG Remote.

Devices like valves, relays, ADCs, DACs, controllers can be supported/controlled.

1-Wire Data (Future Use)

This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required).

Pin Enhanced Remote

1 IO 1 (START REQUEST)

2 IO 2 (STOP)

3 IO 3 (READY)

4 IO 4 (POWER ON)

5 IO 5 (NOT USED)

6 IO 6 (SHUT DOWN)

7 IO 7 (START)

8 IO 8 (PREPARE)

9 1 wire DATA

10 DGND

11 +5 V ERI out

12 PGND

13 PGND

14 +24 V ERI out

15 +24 V ERI out

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12 Hardware Information Interfaces

5V Distribution (Future Use)

Available directly after turning on the hosting module (assures that the firmware can detect certain basic functionality of the device).

For digital circuits or similar.

Provides 500 mA maximum.

Short-circuit proof with automatic switch off (by firmware).

24V Distribution (Future Use)

Available by firmware command (defined turn on/off).

For devices that need higher power

Class 0: 0.5 A maximum (12 W)

Class 1: 1.0 A maximum (24 W)

Class 2: 2.0 A maximum (48 W)

Class depends on hosting modules internal power overhead.

If a connected device requires more power the firmware detects this (overcurrent detection) and provides the information to the user interface.

Fuse used for safety protection (on board).

Short circuit will be detected through hardware.

USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports:

a PC with control software (for example Agilent Lab Advisor)

USB Flash Disk

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 189

12 Hardware Information 2-bit Configuration Switch

2-bit Configuration Switch

Figure 29 Location of Configuration Switch

2-bit configuration switch

Table 30 Dip switches settings for G7116A/B

Mode Select 1 2

Default up up

Coldstart up down

Boot resident down up

Not supported down down

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12 Hardware Information Instrument Layout

Instrument Layout

The industrial design of the module incorporates several innovative features. It uses Agilents E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed. This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet. The advantages of this packaging technology are:

virtual elimination of fixing screws, bolts or ties, reducing the number of components and increasing the speed of assembly/disassembly,

the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations,

the plastic layers help cushion the electronic and mechanical parts from physical shock, and

the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 191

12 Hardware Information Early Maintenance Feedback (EMF)

Early Maintenance Feedback (EMF)

Maintenance requires the exchange of components that are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of use of the module and the analytical conditions, and not on a predefined time interval. The early maintenance feedback (EMF) feature monitors the use of specific components in the instrument, and provides feedback when the user-selectable limits have been exceeded. The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled.

EMF Counters

EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded. Some counters can be reset to zero after the required maintenance procedure.

Using the EMF Counters

The user-settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements. The useful maintenance cycle is dependent on the requirements for use. Therefore, the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument.

Setting the EMF Limits

The setting of the EMF limits must be optimized over one or two maintenance cycles. Initially the default EMF limits should be set. When instrument performance indicates maintenance is necessary, take note of the values displayed by the EMF counters. Enter these values (or values slightly less than the displayed values) as EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 192

13 Appendix

General Safety Information 194 General Safety Information 194 Safety Standards 194 General 194 Before Applying Power 195 Ground the Instrument 195 Do Not Operate in an Explosive Atmosphere 196 Do Not Remove the Instrument Cover 196 Do Not Modify the Instrument 196 In Case of Damage 196 Solvents 197 Safety Symbols 198

Waste Electrical and Electronic Equipment (WEEE) Directive 200

Radio Interference 201

Sound Emission Certification for Federal Republic of Germany 202

Agilent Technologies on Internet 203

This chapter provides addition information on safety, legal and web.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 193

13 Appendix General Safety Information

General Safety Information

General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.

Safety Standards This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards.

General Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions.

WARNING Ensure the proper usage of the equipment.

The protection provided by the equipment may be impaired.

The operator of this instrument is advised to use the equipment in a manner as specified in this manual.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 194

13 Appendix General Safety Information

Before Applying Power

Ground the Instrument

WARNING Wrong voltage range, frequency or cabling

Personal injury or damage to the instrument

Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Make all connections to the unit before applying power.

NOTE Note the instrument's external markings described under Safety Symbols on page 198.

WARNING Missing electrical ground

Electrical shock

If your product is provided with a grounding type power plug, the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard.

The ground pin must be firmly connected to an electrical ground (safety ground) terminal at the power outlet. Any interruption of the protective (grounding) conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 195

13 Appendix General Safety Information

Do Not Operate in an Explosive Atmosphere

Do Not Remove the Instrument Cover

Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product. Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained.

In Case of Damage

WARNING Presence of flammable gases or fumes

Explosion hazard

Do not operate the instrument in the presence of flammable gases or fumes.

WARNING Instrument covers removed

Electrical shock

Do Not Remove the Instrument Cover

Only Agilent authorized personnel are allowed to remove instrument covers. Always disconnect the power cables and any external circuits before removing the instrument cover.

WARNING Damage to the module

Personal injury (for example electrical shock, intoxication)

Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel.

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 196

13 Appendix General Safety Information

Solvents

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 C (392 F). Do not use solvents with a boiling point below 56 C (133 F).

Avoid high vapor concentrations. Keep the solvent temperature at least 40 C (72 F) below the boiling point of the solvent used. This includes the solvent temperature in the sample compartment. For the solvents methanol and ethanol keep the solvent temperature at least 25 C (45 F) below the boiling point.

Do not operate the instrument in an explosive atmosphere.

Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

Reduce the volume of substances to the minimum required for the analysis.

Never exceed the maximum permissible volume of solvents (8 L) in the solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container.

Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

NOTE For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet.

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13 Appendix General Safety Information

Safety Symbols

Table 31 Symbols

The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up.

Sample Cooler unit is designed as vapor-compression refrigeration system. Contains fluorinated greenhouse gas (refrigerant) according to the Kyoto protocol. For specifications of refrigerant, charge capacity, carbon dioxide equivalent (CDE), and global warming potential (GWP) see instrument label.

Flammable Material For Sample Thermostat which uses flammable refrigerant consult Agilent Information Center / User Manual before attempting to install or service this equipment. All safety precautions must be followed.

Confirms that a manufactured product complies with all applicable European Community directives. The European Declaration of Conformity is available at: http://regulations.corporate.agilent.com/DoC/search.htm

Manufacturing date.

Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position

Pacemaker Magnets could affect the functioning of pacemakers and implanted heart defibrillators. A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets.

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13 Appendix General Safety Information

Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

Indicates a pinching or crushing hazard

Indicates a piercing or cutting hazard.

Table 31 Symbols

WARNING A WARNING

alerts you to situations that could cause physical injury or death.

Do not proceed beyond a warning until you have fully understood and met the indicated conditions.

CAUTION A CAUTION

alerts you to situations that could cause loss of data, or damage of equipment.

Do not proceed beyond a caution until you have fully understood and met the indicated conditions.

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13 Appendix Waste Electrical and Electronic Equipment (WEEE) Directive

Waste Electrical and Electronic Equipment (WEEE) Directive

This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste.

NOTE Do not dispose of in domestic household waste

To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information.

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13 Appendix Radio Interference

Radio Interference

Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations.

Test and Measurement

If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

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13 Appendix Sound Emission Certification for Federal Republic of Germany

Sound Emission Certification for Federal Republic of Germany

Sound pressure

Sound pressure Lp <70 db(A) according to DIN EN ISO 7779

Schalldruckpegel

Schalldruckpegel Lp <70 db(A) nach DIN EN ISO 7779

Agilent InfinityLab LC Series Multicolumn Thermostats User Manual 202

13 Appendix Agilent Technologies on Internet

Agilent Technologies on Internet

For the latest information on products and services visit our worldwide web site on the Internet at:

http://www.agilent.com

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In This Book

This manual contains technical reference information about the Agilent 1290 Infinity II Multicolumn Thermostat (G7116B) and Agilent 1260 Infinity II Multicolumn Thermostat (G7116A).

The manual describes the following:

Introduction,

requirements and specifications,

using and optimizing, <