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Agilent Positive Pressure Manifold 96 Processor User Manual PDF

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Summary of Content for Agilent Positive Pressure Manifold 96 Processor User Manual PDF

Agilent Positive Pressure Manifold 48 and 96 Processors

User Manual

Agilent Technologies

Notices Agilent Technologies, Inc. 2017

No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla- tion into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Manual Part Number

G6695-90001

Edition

First edition, August 2017

Printed in USA

Agilent Technologies, Inc. 5301 Stevens Creek Boulevard Santa Clara, CA 95051-7201 USA

The contents are the original instructions supplied by Agilent Technologies.

Warranty

The material contained in this docu- ment is provided as is, and is sub- ject to being changed, without notice, in future editions. Further, to the max- imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par- ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor- mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep- arate agreement shall control.

Safety Notices

CAUTION

A CAUTION notice denotes a hazard. It calls attention to an oper- ating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated condi- tions are fully understood and met.

Contents

Safety and Regulatory Information

Agilent Positive Pressure Manifold Proc

Intended Use 6

Safety and Regulatory Certifications 7

Introduction

Product Description 10

Agilent Positive Pressure Manifold 48 Processor 11

Agilent Positive Pressure Manifold 96 Processor 14

Processor Components 15

Maintaining Manifold Pressure 16

Flow Control 18

Processor Setup

Site Requirements 20

Parts and Accessories 21

Installation 23

Operation

Processor Preparation 26

Sample Processing 26

Maintenance

Bleed off the Processor 30

Tubing Replacement 31

Sealing Gasket Replacement 32

Reference Procedures

Using Your Agilent PPM-48 Processor 34

Using Your Agilent PPM-96 Processor 35

essor User Manual 3

4

Agilent Positive Pressure Manifold Processor User Manual

Agilent Positive Pressure Manifold 48 and 96 Processors User Manual

1 Safety and Regulatory Information

Intended Use 6

Safety and Regulatory Certifications 7

5Agilent Technologies

Safety and Regulatory Information

Intended Use

6

The Agilent positive pressure manifold 48 and 96 processors are used to perform manual sample processing of analytes from various sample types as a stand-alone, independent module for sample preparation. The processor is intended to be used in a professional laboratory setting to perform various manual sample preparation techniques, such as solid phase extraction, using positive pressure application to 1, 3, and 6 mL cartridges and 96-well format filter/extraction plates to prepare samples for downstream testing.

Agilent Positive Pressure Manifold Processor User Manual

Safety and Regulatory Information

Safety and Regulatory Certifications

Declaration of Conformance

Agilent Positive Pressure Manifold Proc

Manufacturer's Name:

Agilent Technologies, Inc.

Manufacturer's Address:

5301 Stevens Creek Boulevard Santa Clara, CA 95051-7201 USA

Declares under sole responsibility that the product as originally delivered

Product Name & Function:

Positive Pressure Manifold Processors

Model & Part Numbers:

5191-4101; 5191-4116

Product Options:

Positive Pressure Manifold 48 Processor Positive Pressure Manifold 96 Processor

complies with the essential requirements of the following applicable European Directives, and carries the CE marking accordingly:

Machinery Directive 2006/42/EC

Contact established in the Community authorized to compile the technical file or the relevant technical documents:

Agilent Technologies Deutschland GmbH Hewlett-Packard Strasse 8, 76337 Waldbronn Germany

essor User Manual 7

Safety and Regulatory Information

Important Safety Warnings

8

There are several important safety notices that you should always keep in mind when using Agilent positive pressure manifold 48 and 96 processors.

When using this product, always wear appropriate Personal

Symbols

WARNING Protective Equipment (PPE) to protect the user from the hazards of the sample being processed, including but not limited to, safety glasses or goggles, lab coat, and gloves.

WARNING Sample and waste materials from use of processor should be disposed of with appropriate waste handling procedures dictated by the hazards of the materials.

Warnings in the manual or on the instrument must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions violates safety standards of design and the intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.

See accompanying instructions for more information.

Indicates a pinching or crushing hazard.

Agilent Positive Pressure Manifold Processor User Manual

Agilent Positive Pressure Manifold 48 and 96 Processors User Manual

2 Introduction

Product Description 10

Agilent Positive Pressure Manifold 48 Processor 11

Agilent Positive Pressure Manifold 96 Processor 14

Processor Components 15

Maintaining Manifold Pressure 16

Flow Control 18

9Agilent Technologies

Introduction

Product Description

10

For sample preparation techniques such as solid phase extraction (SPE), supported liquid extraction (SLE), and filtration (protein precipitation), Agilent positive pressure manifold (PPM) 48 and 96 processors offer:

Uniform flow: Restricted-flow ports ensure consistent gas flow across the manifold, regardless of cartridge or well contents.

No more troublesome stopcocks: The restricted-flow ports eliminate the need for stopcocks, which must be individually and manually controlled.

Easy alignment: The self-adjusting manifold correctly aligns cartridges, test tubes, autosampler vials, and 96-well plates without the need for additional spacers.

Speed and cost efficiency: The 48-cartridge capacity lets you process more samples at once.

Time and resource savings: The autosampler vial collection rack for the PPM-48 allows you to skip the final transfer step.

Greater flexibility: Forced gas supplies a wide range of pressures for processing viscous samples.

Agilent Positive Pressure Manifold Processor User Manual

Introduction

Agilent Positive Pressure Manifold 48 Processor

Agilent Positive Pressure Manifold Proc

Figure 1 Agilent positive pressure manifold 48 processor

Row switches

Manifold

Cartridge rack

Collection rack

Platform

Compression switch

Low flow gauge

Rotameter

Low flow regulator

Flow rate selector

Compression switch

High flow gauge

High flow regulator

Collection tubes or autosampler vials

Rotameter valve

Sealing gasket

The Agilent positive pressure manifold 48 processor (PPM-48) (see Figure 1) allows batch processing of material through up to 48 cartridges.

essor User Manual

11

12

Introduction

A unique feature of the PPM-48 is that each of the four rows is controlled by a switch, located on top of the manifold. These switches may be used to turn off gas flow to individual rows of 12, thus saving gas when those rows are not in use. When the PPM-48 is set to Low Flow, the rotameter only shows the total flow through the turned on rows.

Several 48-position sample collection racks are available to use with the processor. The sample collection racks can accommodate autosampler vials and many common collection tube sizes:

10 75 mm

12 75 mm

13 100 mm

16 100 mm

Contact your local Agilent representative for further details.

The PPM-48 also accommodates multiple types of cartridge racks, for multiple sizes of cartridges, as outlined in Table 1.

If 24 tabbed cartridges are being used in a 6 mL cartridge rack, the cartridges must be oriented in alternating rows (A and C, or B and D) so that the tabs on each cartridge are all vertical and parallel with each other (see Figure 2 on page 13). Incorrect cartridge orientation prevents the cartridges from resting level in the cartridge rack, thus impeding proper gas flow to the cartridges. Gas can be saved by turning off the empty rows.

Table 1 Cartridge rack sizes and capacities

Cartridge size Cartridge rack capacity

1 mL up to 48 cartridges

3 mL up to 48 cartridges

6 mL up to 48 tabless cartridges or 24 tabbed cartridges

Agilent Positive Pressure Manifold Processor User Manual

Introduction

Agilent Positive Pressure Manifold Proc

The sample collection rack is keyed to the platform, and the cartridge racks are keyed to the collection racks, so that sample position is preserved throughout the process. If the material exiting the cartridges is to be disposed, a waste collection rack with a removable waste bin is available to use, instead of a sample collection rack.

Figure 2 6 mL tabbed cartridge orientations

essor User Manual 13

Introduction

Agilent Positive Pressure Manifold 96 Processor

14

Figure 3 Agilent positive pressure manifold 96 processor

Manifold

96-well plate

Collection plate

Platform

Compression switch

Low flow gauge

Rotameter

Low flow regulator

Compression switch

High flow gauge

High flow regulator

Flow rate selector

Rotameter valve

Sealing gasket

One of the benefits of the Agilent positive pressure manifold 96 processor (PPM-96) is the ability to use the processor for both 96-well plates and 1 mL cartridges. Tabless 1 mL cartridges may be assembled in a cartridge holder (p/n 5191-4119). Alternatively, an Agilent VersaPlate base plate (p/n 75400000) can be used with VersaPlate tubes. Both of these options provide the flexibility to create your own customized 96-well plates, expediting method development. This eliminates the need to run multiple 96-well plates with different sorbents.

The PPM-96 is designed to accommodate 1 mL or 2 mL 96-well plates, in both round-well and square-well formats.

Either a sample collection plate, or a waste collection plate may be used under the 96-well plate. If a 1 mL round-well plate is being used with the waste collection plate, a plate holder (p/n 5191-4120) may be used on top of the waste collection plate to stabilize the 96-well plate during processing. The rows are labeled AH, and the columns are labeled 112 on the 96-well plate, tabless cartridge holder, sample collection plate, and VersaPlate to aid in preserving sample position.

Agilent Positive Pressure Manifold P

rocessor User Manual

Introduction

Processor Components

Agilent Positive Pressure Manifold Proc

Table 2 provides a list of the components of the PPM-48 and PPM-96 processors, and a brief description of their functions.

Table 2 Processor components

Component Function

Flow rate selector Controls the operating mode (Off, Low Flow, High Flow).

Compression switches Lowers and raises the manifold.

Rotameter Allows for fine tune flow control in Low Flow mode.

Row toggle switches (PPM-48 only)

Controls the flow to each row of cartridges.

Flow regulators Controls and sets the pressure for both the Low Flow (left knob) and High Flow (right knob) operating modes.

Platform Moves the cartridge stack, or 96-well plate stack, forwards and backwards on the processor. Aligns the manifold with the rack or plate stack.

Low Flow gauge Displays the Low Flow pressure.

High Flow gauge Displays the High Flow pressure.

Manifold Compresses to achieve a seal. Delivers gas through flow restricted ports to cartridges or a 96-well plate.

Sealing gasket Creates a seal between the manifold and either a cartridge rack or a 96-well plate.

essor User Manual 15

Introduction

Maintaining Manifold Pressure

16

The cartridges sorbent beds restrict the sample flow, allowing the flow to increase only as increased pressure is applied (see Figure 4).

Each of the holes in the manifold has a small gas flow restrictor installed. The restrictors allow the manifold to maintain pressure, even when the cartridge rack has empty positions, or as the cartridges elute. As the cartridges start to empty, the restrictors help maintain pressure in the cartridges that still contain fluid, thus allowing processing to continue (see Figure 5 on page 17).

Figure 4 Manifold restrictor design with full cartridges

Input pressure (30 psi)

30 psi 30 psi 30 psi

24 psi 24 psi 24 psi

Manifold pressure chamber

Sealing gasket

Cartridge

Solvent or sample fluid

Sorbent bed

Fluid flows out of cartridge.

Pressure builds up in the cartridge reservoir due to the flow resistance introduced by the sorbent bed.

Gas flow restrictor

Agilent Positive Pressu

re Manifold Processor User Manual

Introduction

Figure 5 Manifold restrictor design with empty cartridge

Input pressure (30 psi)

26 psi 26 psi 26 psi

18 psi 18 psi 10 psi

Manifold pressure remains high despite lack of fluid in the last cartridge.

Empty cartridge

Agilent Positive Pressure Manifold Processor User Manual

17

Introduction

Flow Control

18

When the flow rate selector is set to Off, no gas will flow to the manifold.

The processors each have two gas delivery settings to pressurize the cartridges or 96-well plates.

The Low Flow setting provides a slow, precise gas flow to the manifold. This setting creates a gas flow path through the low flow regulator and the rotameter before entering the manifold. The low flow regulator can be used to set pressures from 0 to 15 psi, and the rotameter can be used to limit flow rates from 0 to 2.5 SCFH.

To avoid damage to the processor, never shut off the rotameter. Only

CAUTION use the flow rate selector to turn the gas flow on or off.

The High Flow setting provides an increased gas flow to the manifold. This setting creates a gas flow path through the high flow regulator directly to the manifold. The high flow regulator can be used to set pressures from 0-100 psi. This setting is commonly used for processing viscous or high particulate samples, or for drying the cartridges during the sample processing procedure.

Agilent Positive Pressure Manifold Processor User Manual

Agilent Positive Pressure Manifold 48 and 96 Processors User Manual

3 Processor Setup

Site Requirements 20

Parts and Accessories 21

Installation 23

19Agilent Technologies

Processor Setup

Site Requirements

20

Ensure your workstation is able to accommodate the specifications of your processor, as shown in Table 3.

Table 3 Processor specifications

Processor

Size

Weight

Operating temperature

Maximum relative humidity

PPM-48 33.5 30.5 39 cm (13.2 12 15.4 inches)

13.4 kg (29.6 lbs) 5 C to 40 C (41 F to 104 F)

80%

PPM-96 27.1 30.5 32.5 cm (10.7 12.0 12.8 inches)

11.0 kg (24.2 lbs) 5 C to 40 C (41 F to 104 F)

80%

The processors are not supplied with a filter for the source gas

CAUTION input. Use a clean, oil-free gas source to help prevent sample contamination.

If you are using a filter with your processor, ensure your workstation can accommodate both the processor and filter. Agilent recommends using a BHT-4 (1/4 inch fittings) or BHT-2 (1/8 inch fittings) filter to help prevent sample contamination. The filter must be positioned vertically, and is not self-supporting.

The processors are designed to be operated only indoors.

Always perform sample processing in an area with appropriate

WARNING environmental controls (ventilation, additional enclosures) dictated by the hazards of the samples being processed.

Agilent recommends using compressed nitrogen as the gas supply for your processor. If compressed nitrogen is not available, the processors can operate using high purity filtered instrument air. Table 4 shows the optimum, minimum, and maximum input pressures for your processor.

Table 4 Processor input pressures

Optimum source pressure Minimum pressure Maximum pressure

80 psig 60 psig 100 psig

Agilent Positive Pressure Manifold Processor User Manual

Processor Setup

Parts and Accessories

Agilent Positive Pressure Manifold Proc

Table 5 and Table 6 display the parts included with the PPM-48 (p/n 5191-4101) and PPM-96 (p/n 5191-4116) respectively. Table 7 and Table 8 on page 22 show additional accessories that may be ordered individually.

The installation kits provided with the PPM-48 and PPM-96 are identical, and include the following:

Polyethylene tubing (6, 1/8 i.d., 1/4 o.d.)

1/8 NPT connectors

1/4 NPT connectors

Table 7 lists the accessories that may be used with the PPM-48 processor.

Table 5 Agilent positive pressure manifold 48 processor included parts

Part Part number

Waste rack with three waste bins 5191-4112

Installation kit 5191-4114

User Manual G6695-90001

Table 6 Agilent positive pressure manifold 96 processor included parts

Part Part number

Single well waste plate 5191-4121

Holder for plates 5191-4120

Installation kit 5191-4114

User Manual G6695-90001

Table 7 Agilent positive pressure manifold 48 processor accessories

Description Part number

Cartridge racks 1 mL cartridge rack 5191-4102

3 mL cartridge rack 5191-4103

6 mL cartridge rack 5191-4104

essor User Manual 21

22

Processor Setup

Table 8 lists the accessories that may be used with the PPM-96 processor.

Collection racks 10 75 mm tubes 5191-4105

12 75 mm tubes 5191-4106

13 100 mm tubes 5191-4107

16 100 mm tubes 5191-4108

12 32 mm autosampler vials 5191-4109

Additional accessories Sealing gasket for PPM-48 5191-4110

Waste bin for PPM-48, 3/pk 5191-4113

Gas traps Big Hydrocarbon Trap (1/4 inch fittings) BHT-4

Big Hydrocarbon Trap (1/8 inch fittings) BHT-2

Table 8 Agilent positive pressure manifold 96 processor accessories

Description Part number

Collection plates and sealing mats

Square-well collection plate, 350 L, 50/pk

5133007

Square-well collection plate, 1 mL, 50/pk

5133008

Square-well collection plate, 2 mL, 50/pk

5133009

Square 96-well sealing mat, 50/pk 5133005

Captiva 96-deep well collection plate, 1 mL, 10/pk

A696001000

Captiva pierceable 96-deep well collection plate cover,10/pk

A8961007

Additional accessories Sealing gasket for PPM-96 5191-4117

1 mL tabless cartridge holder for PPM-96

5191-4119

Single well waste plate 5191-4121

Gas traps Big Hydrocarbon Trap (1/4 inch fittings) BHT-4

Big Hydrocarbon Trap (1/8 inch fittings) BHT-2

Table 7 Agilent positive pressure manifold 48 processor accessories (continued)

Description Part number

Agilent Positive Pressure Manifold Processor User Manual

Processor Setup

Installation

Agilent Positive Pressure Manifold Proc

1 Set the processor on your workbench, near your gas source. See Site Requirements on page 20 for information about selecting an appropriate source.

When installing a new processor, only use the tubing provided in

WARNING the installation kit (p/n 5191-4114).

2 Ensure the flow rate selector is turned to Off, and that your gas source is off.

3 Cut the provided tubing to the desired lengths. If the cut is not clean and even, repeat (see Figure 6).

4 If you are using a filter with your processor, use the tubing provided to attach the filter to your gas source. Agilent recommends using a BHT-4 (1/4 inch fittings) or BHT-2 (1/8 inch fittings) filter to help prevent sample contamination.

5 Connect the filter to the processor gas inlet, so that gas flows through the filter prior to entering the processor. Push the tubing into the push-to-connect fitting until it is fully seated in the fitting. See Figure 7 on page 24 and Figure 8 on page 24 for diagrams showing proper tubing installation. If you are not using a filter, use the tubing provided to connect your gas source directly to your processors gas inlet.

Figure 6 Properly cut tubing

essor User Manual 23

24

Processor Setup

Figure 7 Agilent positive pressure manifold 48 processor gas connections

Figure 8 Agilent positive pressure manifold 96 processor gas connections

Gas inletOut to Manifold

From gas source or filter

Out to Manifold

From gas source or filter

Gas inlet

Agilent Positive Pressure Manifold Proc

essor U
ser Manual

Agilent Positive Pressure Manifold 48 and 96 Processors User Manual

4 Operation

Processor Preparation 26

Sample Processing 26

25Agilent Technologies

Operation

Processor Preparation

26

1 Ensure that the processor is connected to a gas supply, and that the flow rate selector is turned to Off. See Site Requirements on page 20 and Installation on page 23 for more information.

2 Turn on your gas supply.

Sample Processing

For a summary of this procedure that may be kept near your processor and referenced, see Using Your Agilent PPM-48 Processor on page 34 and Using Your Agilent PPM-96 Processor on page 35.

For specific workflow guidelines and sample protocols, refer to A Technical Guide to Agilent Positive Pressure Manifold 48 and 96 Processors (5991-8151EN).

1 Slide the platform out from under the manifold.

2 Assemble the stack (see Figure 9 and Figure 10).

3 Place the stack on top of the platform. When positioned correctly, the stack should sit level on the platform.

Figure 9 Agilent positive pressure manifold 48 processor stack

Figure 10 Agilent positive pressure manifold 96 processor stack

Cartridge rack

Sample collection rack or waste bin rack

96-well plate

Optional holder for plates

Sample collection plate or waste collection plate

Agilent Positive Pressure Manifold

Processor User Manual

Operation

Agilent Positive Pressure Manifold Proc

4 If you are using a PPM-48, flip the row switches on top of the manifold down to open the desired row valves.

5 Turn the flow rate selector from Off to Low Flow, to release any excess manifold pressure.

6 Adjust the flow regulator by pulling out on the knob, turning it to the desired pressure, then pushing it back in to lock it in place.

To avoid damage to the processor, never shut off the rotameter. Only

CAUTION use the flow rate selector to turn the gas flow on or off.

7 Without closing the rotameter, adjust the rotameter valve to achieve the desired flow rate. It is recommended to start with a low flow rate, and then increase the rate as necessary.

8 Turn the flow rate selector to Off.

9 Add solvent or sample to each cartridge or well, as required by your method.

Solvent or sample spillage should be cleaned immediately to prevent

CAUTION damage to the processor.

10 Slide the collection platform under the manifold, until it reaches the stops.

Ensure the flow rate selector is set to Off whenever raising or

CAUTION lowering the manifold.

WARNING When compressing or decompressing the cartridges, keep all body parts, hair, loose clothing, and extraneous objects away from the manifold.

11 Simultaneously press down on the two compression switches, until the manifold lowers completely. If the switches are released early, the manifold will rise. This is an intentional safety feature to keep your hands away from parts that move during the compression and decompression cycles. The compression and decompression speeds are preset and not adjustable.

12 Turn the flow rate selector to either Low Flow or High Flow, as required by your method.

essor User Manual 27

28

Operation

13 Readjust the rotameter valve to achieve the desired liquid flow rate, adjusting as necessary throughout sample processing.

14 Once processing is complete, turn the flow rate selector to Off.

15 Simultaneously press up on the two compression switches until the manifold rises completely.

16 Pull the platform out from under the manifold, until it reaches the stops at the front of the processor.

17 Remove and separate the stack.

18 Repeat as required by your method.

19 Clean the silicone rubber sealing gasket with methanol.

Agilent Positive Pressure Manifold Processor User Manual

Agilent Positive Pressure Manifold 48 and 96 Processors User Manual

5 Maintenance

Bleed off the Processor 30

Tubing Replacement 31

Sealing Gasket Replacement 32

29Agilent Technologies

Maintenance

Bleed off the Processor

30

After the gas supply has been shut off, the processor will remain pressurized until the pressure has been released. Agilent recommends bleeding off this excess pressure when the processor is not in use or if the processor will sit for an extended period. Situations where bleeding off the processor is necessary include, but are not limited to:

The processor is being relocated or moved

Maintenance is being performed

Tubing is being disconnected/replaced

The gas supply or filter is being changed

To bleed off the processor:

1 Turn off the gas supply.

2 If you are using a PPM-48, flip all four row switches down to open the row valves.

3 Turn the flow rate selector from Off to High Flow.

4 Wait until the pressure returns to 0 psi. Both pressure gauges should read "0", and there should be no audible hissing noise coming from the processor.

5 Turn the flow rate selector to Off.

Agilent Positive Pressure Manifold Processor User Manual

Maintenance

Tubing Replacement

Agilent Positive Pressure Manifold Proc

To replace the polyethylene tubing:

1 Bleed off the processor (see Bleed off the Processor on page 30).

2 Push in the outer ring of the push-to-connect fitting, while simultaneously pulling the tubing out of the fitting.

When installing a new processor, or replacing tubing, only use the

WARNING tubing provided in the installation kit supplied with your processor.

3 Push the new tubing from an installation kit (p/n 5191-4114) into the appropriate push-to-connect fittings (see Figure 7 on page 24 and Figure 8 on page 24). For more information on the installation kit, see Parts and Accessories on page 21.

essor User Manual 31

Maintenance

Sealing Gasket Replacement

32

Replacement instructions are provided with each replacement sealing gasket. If the instructions provided differ from those listed here, follow the provided instructions. Agilent recommends replacing the sealing gasket every 6 to 12 months, depending on frequency of use, wear, and solvents used.

To replace the sealing gasket:

1 Bleed off the processor (see Bleed off the Processor on page 30).

2 Use the included 3/16 hex key to remove the two 1/4-20 screws fastening the manifold to the processor.

3 Lift the manifold from the standoffs, and place it upside down on the table.

4 Remove the old sealing gasket from the manifold.

5 Remove the old adhesive by wiping the surface areas with a lint-free cloth, dampened with either acetone or isopropanol.

Remove all adhesive residue from the manifold prior to installation

CAUTION of the new sealing gasket. Do not clog the holes of the manifold when removing old adhesive.

6 Remove the backing from the replacement sealing gasket.

7 For the PPM-96, insert two of the included screws into two opposite corners of the sealing gasket, and partially screw them into the threaded holes. Place the additional two screws in the remaining corners, and carefully adhere the replacement sealing gasket to the manifold, ensuring all ports are visible.

For the PPM-48, align the replacement seal with the manifold cartridge holes, and adhere it to the manifold.

8 On the PPM-96, once the sealing gasket is attached, remove the four screws.

9 Carefully set the manifold on the standoffs.

10 Attach the manifold to the processor using the 3/16 hex key and 1/4-20 screws.

Agilent Positive Pressure Manifold Processor User Manual

Agilent Positive Pressure Manifold 48 and 96 Processors User Manual

6 Reference Procedures

Using Your Agilent PPM-48 Processor 34

Using Your Agilent PPM-96 Processor 35

The following operating procedures can be printed and kept next to your processor for easy reference. For more details on operating your processor, see Sample Processing on page 26, or A Technical Guide to Agilent Positive Pressure Manifold 48 and 96 Processors (5991-8151EN).

For important safety warnings that must be observed during processor operation, see Important Safety Warnings on page 8.

33Agilent Technologies

Reference Procedures

Using Your Agilent PPM-48 Processor

For important safety warnings that must be observed during processor operation, see Important Safety Warnings on page 8. For a more detailed operating procedure, see Sample Processing on page 26.

1 Assemble the cartridge stack and place it onto the platform.

2 Use the row switches to open the desired row valves.

3 Adjust the low flow regulator to achieve the desired pressure.

4 Add solvent/sample to the cartridges.

5 Slide the platform back until it reaches the stops.

CAUTION Solvent or sample spillage should be cleaned immediately to prevent damage to the processor.

w rate selector is set to Off whenever raising or

6 Lower the manifold.

7 Turn the flow rate selector from Off to Low Flow.

8 Adjust the rotameter to achieve the desired flow rate.

9 After elution, turn the flow rate selector to Off, and raise the manifold.

10 Slide the platform forward, and repeat steps 3-9 per protocol.

11 If a dry step is required: After elution of the solvent, turn the flow rate selector from Low Flow to High Flow and adjust the high flow regulator to achieve the desired pressure.

12 Collection step: Replace the waste rack with the appropriate collection rack and test tubes or autosampler vials. Use gravity or positive pressure (flow rate selector set to Low Flow), based on your specific protocol. Sample extracts may be diluted, dried down and reconstituted, or directly analyzed, based on your protocol.

13 Clean the silicone rubber sealing gasket with methanol.

CAUTION Ensure the flo lowering the manifold.

WARNING When compressing or decompressing the cartridges, keep all body parts, hair, loose clothing, and extraneous objects away from the manifold.

CAUTION To avoid damage to the processor, never shut off the rotameter. Only use the flow rate selector to turn the gas flow on or off.

34Agilent Technologies

Reference Procedures

Using Your Agilent PPM-96 Processor

For important safety warnings that must be observed during processor operation, see Important Safety Warnings on page 8. For a more detailed operating procedure, see Sample Processing on page 26.

1 Assemble the plate stack, and place it onto platform.

2 Adjust the low flow regulator to achieve the desired pressure.

3 Add solvent/sample to the wells.

4 Slide the platform back until it reaches the stops.

CAUTION Solvent or sample spillage should be cleaned immediately to prevent damage to the processor.

w rate selector is set to Off whenever raising or

5 Lower the manifold.

6 Turn the flow rate selector from Off to Low Flow.

7 Adjust the rotameter to achieve the desired flow rate.

8 After elution, turn the flow rate selector to Off, and raise the manifold.

9 Slide the platform forward, and repeat steps 3-8 per protocol.

10 If a dry step is required: After elution of the solvent, turn the flow rate selector from Low Flow to High Flow, and adjust the high flow regulator to achieve the desired pressure.

11 Collection step: Replace the single well waste plate with the appropriate collection plate. Use gravity or positive pressure (flow rate selector set to Low Flow), based on your specific protocol. Sample extracts may be diluted, dried down and reconstituted, or directly analyzed, based on your protocol.

12 Clean the silicone rubber sealing gasket with methanol.

CAUTION Ensure the flo lowering the manifold.

WARNING When compressing or decompressing the cartridges, keep all body parts, hair, loose clothing, and extraneous objects away from the manifold.

CAUTION To avoid damage to the processor, never shut off the rotameter. Only use the flow rate selector to turn the gas flow on or off.

35Agilent Technologies

36

Reference Procedures

Agilent Positive Pressure Manifold Processor User Manual

Agilent Technologies

Agilent Technologies, Inc.

Printed in USA, August 2017

*G6695-90001*

G6695-90001

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