Agilent InfinityLab LC Series 1290 Infinity II Modulator User Manual PDF

Agilent InfinityLab LC Series 1290 Infinity II MS Flow Modulator

User Manual

MS Flow Modulator User Manual

Notices Document Information Document No: SD-29000231 Rev. C Part No: G7170-90000 Rev. C EDITION 10/2019

Copyright Agilent Technologies, Inc. 2017-2019

No part of this manual may be repro- duced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written con- sent from Agilent Technologies, Inc. as governed by United States and interna- tional copyright laws.

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Warranty The material contained in this document is provided as is, and is subject to being changed, without notice, in future edi- tions. Further, to the maximum extent permitted by applicable law, Agilent dis- claims all warranties, either express or implied, with regard to this manual and any information contained herein, includ- ing but not limited to the implied warran- ties of merchantability and fitness for a particular purpose. Agilent shall not be lia- ble for errors or for incidental or conse- quential damages in connection with the furnishing, use, or performance of this document or of any information con- tained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Restricted Rights Legend U.S. Government Restricted Rights. Soft- ware and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Soft- ware and technical data pursuant to FAR 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Depart- ment of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commer- cial Computer Software or Computer Software Documentation).

Safety Notices

CAUTION A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARN- ING notice until the indicated conditions are fully understood and met.

MS Flow Modulator User Manual

In This Guide

This manual covers the Agilent InfinityLab LC Series 1290 Infinity II MS Flow Modulator (G7170B)

1 Introduction

This chapter gives an introduction to the module.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical and performance specifications.

3 Installing the Module

This chapter gives information about the installation of the valve drive and the valve heads.

4 Using the Module

This chapter provides information on how to use the module.

5 Troubleshooting and Diagnostics

Overview about the troubleshooting and diagnostic features.

6 Error Information

This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

7 Maintenance

This chapter describes the maintenance of the module.

8 Parts and Materials

This chapter provides information on parts for maintenance.

MS Flow Modulator User Manual 3

9 Identifying Cables

This chapter provides information on cables used with the modules.

10 Hardware Information

This chapter describes the module in more detail on hardware and electronics.

11 Appendix

This chapter provides addition information on safety, legal and web.

MS Flow Modulator User Manual 4

Contents

1 Introduction 8

1290 Infinity II MS Flow Modulator 9 Features (G7170B) 10 Leak and Waste (G7170B) 11 Operating Principle 12

2 Site Requirements and Specifications 14

Site Requirements 15 Physical Specifications 18 Performance Specifications 19

3 Installing the Module 20

Unpacking the Module 21 Installation of the MS Flow Modulator 22

4 Using the Module 31

Overview 32 Solvent Information 33 Status Indicator 40 Leak and Waste Handling 41 Configure the Flow Modulator 43

5 Troubleshooting and Diagnostics 46

User Interfaces 47 Agilent Lab Advisor Software 48

MS Flow Modulator User Manual 5

6 Error Information 49

What Are Error Messages 50 General Error Messages 51 Module Specific Error Messages 56

7 Maintenance 58

Introduction to Maintenance 59 Warnings and Cautions 60 Cleaning the Module 61 Replacing the Fuses of the Infinity Valve Drive 62 Replace Rotor and Stator 64 Replace Valve Heads 67 Replacing the Module Firmware 71

8 Parts and Materials 73

Accessory Kit for the Flow Modulator 74 Optional Parts 76

9 Identifying Cables 77

Cable Overview 78 Analog Cables 80 Remote Cables 82 CAN/LAN Cables 86 Agilent Module to PC 87 USB 88

MS Flow Modulator User Manual 6

In This Guide

10 Hardware Information 89

Electrical Connections 90 Interfaces 92 Config Switch Settings of the Infinity Valve Drive 98

11 Appendix 100

General Safety Information 101 Waste Electrical and Electronic Equipment (WEEE) Directive 108 Radio Interference 109 Sound Emission 110 Agilent Technologies on Internet 111

MS Flow Modulator User Manual 7

1 Introduction

1290 Infinity II MS Flow Modulator 9 Features (G7170B) 10 Leak and Waste (G7170B) 11 Operating Principle 12

This chapter gives an introduction to the module.

MS Flow Modulator User Manual 8

1 Introduction 1290 Infinity II MS Flow Modulator

1290 Infinity II MS Flow Modulator

Based on technology in our InfinityLab Quick Change valves, the 1290 Infinity II MS Flow Modulator facilitates efficient flow control for mass-based fraction collection on all InfinityLab LC Purification Solutions. The Flow Modulator is a compact module suitable for flow rates up to 100 mL/min and supports split ratios between 40:1 and 400000:1 with split frequencies up to 2 Hz.

Figure 1 Overview of the MS flow modulator

Status indicator

Valve

MS Flow Modulator User Manual 9

1 Introduction Features (G7170B)

Features (G7170B)

Purification efficiency Supports wide range of split modes from 40:1 up to 400000:1 depending on

flow rate Enables split frequencies of up to 2 Hz Handles flow rates up to 100 mL/min Instrument efficiency Integrates into any InfinityLab LC Purification Solution through CAN-bus

technology Supported by Agilent drivers no additional software or macros required Laboratory efficiency Compact design and easy connection to any InfinityLab LC Purification

Solution saves bench space Compatible with all InfinityLab LC Purification Solutions, enabling upgrade to

mass-based fraction collection when needs demand Integrates seamlessly in OpenLAB CDS ChemStation for familiar and reliable

operation

MS Flow Modulator User Manual 10

1 Introduction Leak and Waste (G7170B)

Leak and Waste (G7170B)

1

NOTE This example shows the leak and waste concept of the module, if installed to a leak plane and attached to another module.

Alternatively, the module can be installed and integrated to the leak and waste concept of 1290 Infinity II Preparative Col- umn Compartment.

Tube to waste

Leak plane

MS Flow Modulator User Manual 11

1 Introduction Operating Principle

Operating Principle

The flow modulator is a compact, active splitting device configured to deliver three different unique aliquots from a HPLC to a Single Quad Mass Spectroscopic Detector while maintaining uninterrupted flow.

Aliquot Transfer Principle of aliquot transfer (see also Figure 2 on page 12): 1 In the Aliquot Fill position, HPLC effluent enters Port 1 where a portion of the

stream is directed into one of the MS Flow Modulators volume transfer chambers.

2 The LC stream exits from Port 2 and may be directed to a second detector or a fraction collector.

3 A Make-up pump connects to Port 3 and, in the Aliquot Transfer position, the volume captured is transferred through Port 4 into the analytical stream of the MS.

Figure 2 Schematics of an active flow splitter, showing the fill and transfer positions of the valve

MS Flow Modulator User Manual 12

1 Introduction Operating Principle

Calculation of the Split Ratio

The MS Flow Modulator has 4 ports with 6 positions (2 x 3) The user can choose the size of aliquots, from three aliquots The valve will move the rotor between two of the same aliquots, backwards

and forwards The user can change the gating frequency of the valve from 14 different modes The split ratio, resulting from the selected mode and the prep flow rate, is calculated internally and displayed in the method.

Port

2 grooves per for each aliquot

Groove

MS Flow Modulator User Manual 13

2 Site Requirements and Specifications

Site Requirements 15 Physical Specifications 18 Performance Specifications 19

This chapter provides information on environmental requirements, physical and performance specifications.

MS Flow Modulator User Manual 14

2 Site Requirements and Specifications Site Requirements

Site Requirements

A suitable environment is important to ensure optimal performance of the instrument.

Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 18. Consequently there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.

WARNING Hazard of electrical shock or damage of your instrumentation

can result, if the devices are connected to a line voltage higher than specified.

Connect your instrument to the specified line voltage only.

WARNING Electrical shock hazard

The module is partially energized when switched off, as long as the power cord is plugged in.

The cover protects users from personal injuries, for example electrical shock.

Do not open the cover.

Do not operate the instrument and disconnect the power cable in case the cover has any signs of damage.

Contact Agilent for support and request an instrument repair service.

WARNING Inaccessible power plug.

In case of emergency it must be possible to disconnect the instrument from the power line at any time.

Make sure the power connector of the instrument can be easily reached and unplugged.

Provide sufficient space behind the power socket of the instrument to unplug the cable.

MS Flow Modulator User Manual 15

2 Site Requirements and Specifications Site Requirements

Power Cords Country-specific power cords are available for the module. The female end of all power cords is identical. It plugs into the power-input socket at the rear. The male end of each power cord is different and designed to match the wall socket of a particular country or region.

Agilent makes sure that your instrument is shipped with the power cord that is suitable for your particular country or region.

WARNING Unintended use of power cords

Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment.

Never use a power cord other than the one that Agilent shipped with this instrument.

Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

WARNING Absence of ground connection

The absence of ground connection can lead to electric shock or short circuit.

Never operate your instrumentation from a power outlet that has no ground connection.

WARNING Electrical shock hazard

Solvents may damage electrical cables.

Prevent electrical cables from getting in contact with solvents.

Exchange electrical cables after contact with solvents.

MS Flow Modulator User Manual 16

2 Site Requirements and Specifications Site Requirements

Condensation

CAUTION Condensation within the module

Condensation can damage the system electronics.

Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module.

If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation.

MS Flow Modulator User Manual 17

2 Site Requirements and Specifications Physical Specifications

Physical Specifications

Table 1 Physical Specifications

Type Specification Comments

Weight 2.2 kg (4.9 lbs)

Dimensions (height width depth)

95 x 95 x 300 mm (3.7 x 3.7 x 11.8 inches)

Line voltage 100 240 V~, 10 % Wide-ranging capability

Line frequency 50 or 60 Hz, 5 %

Power consumption 20 VA, 4 W

Ambient operating tempera- ture

4 40 C (39 104 F)

Ambient non-operating tem- perature

-40 70 C (-40 158 F)

Humidity < 95 % r.h. at 40 C (104 F) Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Non-operating altitude Up to 4600 m (15092 ft) For storing the module

Safety standards: IEC, EN, CSA, UL

Installation category II, Pollution degree 2 For indoor use only.

ISM Classification ISM Group 1 Class B According to CISPR 11

MS Flow Modulator User Manual 18

2 Site Requirements and Specifications Performance Specifications

Performance Specifications

Table 2 Performance Specifications 1290 Infinity II MS Flow Modulator (G7170B)

Feature Specification Comment

External Leak Sensor Yes controlled via ERI

Flow rate 4 200 mL/min

Maximum Pressure 69 bar (100 psi)

Valve Options 5067-4292 Quick-Change valve head (user-exchangeable)

Valve Identification Via RFID tag

Instrument Control LC & CE Drivers A.02.17 or above Instrument Control Framework (ICF) A.02.04 or above Instant Pilot (G4208A) with firmware B.02.22 or above InfinityLab LC Companion (G7108A) Lab Advisor B.02.10 or above

For details about sup- ported software versions refer to the compatibility matrix of your version of the LC and CE Drivers

Communications CAN (2x) G7170B is a hosted mod- ule. The LC stack needs to contain a LAN card for communication and con- trol.

Maintenance and safety-related features

Leak handling with external leak sensor and leak pane. Leak detection, safe leak handling, leak output signal for shutdown of pumping system, and low voltages in major mainte- nance areas Extensive diagnostics, error detection and dis- play with Agilent Lab Advisor software

GLP features Early maintenance feedback (EMF) for continu- ous tracking of instrument usage with user-set- table limits and feedback messages. Electronic records of maintenance and errors

Housing All materials recyclable

MS Flow Modulator User Manual 19

3 Installing the Module

Unpacking the Module 21 Installation of the MS Flow Modulator 22 Assembling and Installing the Leak Tray and Leak Sensor 23 Mounting the MS Flow Modulator to an Instrument 24 Mount the MS Flow Modulator to the 1290 Infinity II Preparative Column Compartment. 25 Install the MS Flow Modulator 26 Install the valve head and connecting capillaries 28

This chapter gives information about the installation of the valve drive and the valve heads.

MS Flow Modulator User Manual 20

3 Installing the Module Unpacking the Module

Unpacking the Module

Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment.

Delivery Checklist Ensure all parts and materials have been delivered with your module. The delivery checklist is included to your shipment. For parts identification please check the illustrated parts breakdown in the chapter Parts and Materials. Please report any missing or damaged parts to your local Agilent Technologies sales and service office.

Accessory Kit The flow modulator is shipped with Accessory Kit (G7170-68705) (see Accessory Kit for the Flow Modulator on page 74).

CAUTION "Defective on arrival" problems

If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.

Notify your Agilent sales and service office about the damage.

An Agilent service representative will inspect the instrument at your site and initiate appropriate actions.

MS Flow Modulator User Manual 21

3 Installing the Module Installation of the MS Flow Modulator

Installation of the MS Flow Modulator

The MS Flow Modulator can be installed in different ways. Mounted to another module

Inside the 1290 Infinity II Preparative Column Compartment

NOTE The MS Flow Modulator fits on Infinity I rails or Infinity II clamps.

MS Flow Modulator User Manual 22

3 Installing the Module Installation of the MS Flow Modulator

Assembling and Installing the Leak Tray and Leak Sensor

NOTE Only connect or disconnect the leak sensor while the module is powered off.

The module must be powered off for at least 10 s to recognize any hardware changes correctly.

1 Attach the leak tray clamp to the leak tray. 2 Push the leak sensor into its holder.

3 Slide the leak tray onto the bottom of the MS Flow Mod- ulator.

4 Connect the leak sensor cable to the valve drive.

MS Flow Modulator User Manual 23

3 Installing the Module Installation of the MS Flow Modulator

Mounting the MS Flow Modulator to an Instrument

CAUTION "Defective on arrival" problems

If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.

Notify your Agilent sales and service office about the damage.

An Agilent service representative will inspect the instrument at your site and initiate appropriate actions.

1 Ensure the power switch at the rear of the module is OFF and the power connector is unplugged.

2 Identify the marks for the screws on the side panel of the cabinet assy and screw the clamp guides to the side panel of the cabinet.

3 Slide the flow modulator into the clamp guides from front to back.

4 Attach the waste tubing to the leak plane and guide it to a proper waste container.

MS Flow Modulator User Manual 24

3 Installing the Module Installation of the MS Flow Modulator

Mount the MS Flow Modulator to the 1290 Infinity II Preparative Column Compartment.

1 Slide the MS Flow Modulator into a free slot of the 1290 Infinity II Preparative Column Compartment. Position the mod- ule correctly above the leak tray.

MS Flow Modulator User Manual 25

3 Installing the Module Installation of the MS Flow Modulator

Install the MS Flow Modulator

Software required Agilent OpenLab CDS A.02.02/ C.01.07 SR3 HF1 LC drivers A.02.17 LabAdvisor B.02.10

Firmware required Description Firmware Set: B/C/D.07.20

1 Mount the flow modulator to another module, see Mounting the MS Flow Modulator to an Instrument on page 24.

OR

Slide the flow modulator into a free slot inside the col- umn compartment, see Mount the MS Flow Modulator to the 1290 Infinity II Preparative Column Compartment. on page 25.

2 Plumb the UV-Detector, Fraction Collector, Make-up Pump and MSD lines to the 4 ports of the MS Flow Mod- ulator.

NOTE Use the finger tight 10-32 male threaded UHPFF fittings only, that are supplied with the splitter.

Note the different length for each fitting.

3 Connect the power cable to the power connector at the rear of the module.

4 Connect the CAN interface connection.

From make-up pump

From LC detector

To MSD

To fraction collector

or valve module

or column

MS Flow Modulator User Manual 26

3 Installing the Module Installation of the MS Flow Modulator

Next Steps:

5 Power on the flow modulator by switching the power button at the rear of the module.

NOTE To power cycle the module (necessary for example after a valve head, or leak sensor installation), wait for at least 10 sec- onds before re-powering.

MS Flow Modulator User Manual 27

3 Installing the Module Installation of the MS Flow Modulator

Install the valve head and connecting capillaries

CAUTION The valve actuator contains sensitive optical parts, which need to be protected from dust and other pollution. Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results.

Always install a valve head for operation and storage. For protecting the actuator, a dummy valve head can be used instead of a functional valve. Do not touch parts inside the actuator.

NOTE For a correct installation of the valve head, the outside pin (red) must completely fit into the outside groove on the valve drives shaft (red). A correct installation is only possible if the two pins (green and blue) on the valve head fit into their corresponding grooves on the valve drives actuator axis. Their match depends on the diameter of the pin and groove.

NOTE The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module. Valve properties will not be updated, if the valve head is replaced while the module is on. Selection of valve port positions can fail, if the instrument does not know the properties of the installed valve.

NOTE To allow correct valve identification, power off the module for at least 10 s.

NOTE For firmware requirements see Information on new RFID Tag Assembly Version Technical Note (01200-90130) which is included to each valve head.

MS Flow Modulator User Manual 28

3 Installing the Module Installation of the MS Flow Modulator

1 Insert the valve head into the valve shaft.

OR If the outside pin does not fit into the outside groove, you have to turn the valve head until you feel that the two pins snap into the grooves. Now you should feel additional resis- tance from the valve drive while continuously turning the valve head until the pin fits into the groove.

2 When the outer pin is locked into the groove, manually screw the nut onto the valve head.

NOTE Fasten the nut with the 5043-1767 Valve Removal tool.

MS Flow Modulator User Manual 29

3 Installing the Module Installation of the MS Flow Modulator

3 Install all required capillary connections to the valve. 4 Power on or power-cycle your module, so the valve head gets recognized during module initialization.

MS Flow Modulator User Manual 30

4 Using the Module

Overview 32 Solvent Information 33 Material Information 35

Status Indicator 40 Leak and Waste Handling 41 Waste Concept 42

Configure the Flow Modulator 43

This chapter provides information on how to use the module.

MS Flow Modulator User Manual 31

4 Using the Module Overview

Overview

The flow modulator is a compact, active splitting device capable of automated control. For details, see Operating Principle on page 12.

The split ratio depends on the main flow rate, the rotor seal groove size (aliquot volume) and the frequency (aliquots gated to the MS):

Use the controller software to establish the required split ratio, see Configure the Flow Modulator on page 43.

MS Flow Modulator User Manual 32

4 Using the Module Solvent Information

Solvent Information

Chemical Compatibility

Observe the following recommendations on the use of solvents. Follow the recommendations for avoiding the growth of algae, see the pump

manuals. Small particles can permanently block capillaries and valves. Therefore,

always filter solvents through 0.22 m filters. Avoid or minimize the use of solvents that may corrode parts in the flow path.

Consider specifications for the pH range given for different materials such as flow cells, valve materials etc. and recommendations in subsequent sections.

CAUTION Chemical compatibility of the valve parts

Corrosion of stator seal and rotor seal.

Do not use sulfuric acid and generally avoid the use of concentrated acids.

Use with care substances as Chlorobenzene, Chloroform, Dichlormethane, Dimethyl Sulfoxid, Toluene, and Ethyl Acetate.

MS Flow Modulator User Manual 33

4 Using the Module Solvent Information

Solvent compatibility for stainless steel in standard LC systems Stainless steel is inert to many common solvents. It is stable in the presence of acids and bases in the pH range specified for standard HPLC (pH 1 12.5). It can be corroded by acids below pH 2.3. In general, the following solvents may cause corrosion and should be avoided with stainless steel: Solutions of alkali halides, their respective acids (for example, lithium iodide,

potassium chloride, and so on) and aequous solutions of halogenes High concentrations of inorganic acids such as nitric acid, sulfuric acid, and

organic solvents, especially at higher temperatures (if your chromatography method allows, replace by phosphoric acid or phosphate buffer which are less corrosive to stainless steel).

Halogenated solvents or mixtures that form radicals and/or acids, for example: 2 CHCl3 + O2 2 COCl2 + 2 HCl

This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.

Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides.

Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel.

Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid).

Mixtures of carbon tetrachloride with 2-propanol or THF.

MS Flow Modulator User Manual 34

4 Using the Module Solvent Information

Material Information Materials in the flow path are carefully selected based on Agilents experiences in developing highest quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.

Disclaimer

Subsequent data was collected from external resources and is meant as a reference. Agilent cannot guarantee the correctness and completeness of such information. Data is based on compatibility libraries, which are not specific for estimating the long-term life time under specific but highly variable conditions of UHPLC systems, solvents, solvent mixtures and samples. Information can also not be generalized due to catalytic effects of impurities like metal ions, complexing agents, oxygen etc. Apart from pure chemical corrosion, other effects like electro corrosion, electrostatic charging (especially for non-conductive organic solvents), swelling of polymer parts etc. need to be considered. Most data available refers to room temperature (typically 20 25 C, 68 77 F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials.

PEEK

PEEK (Polyether-Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is therefore the material of choice for UHPLC and biochemical instrumentation.

It is stable in the specified pH range (for the Bio-inert LC system: pH 1 13, see bio-inert module manuals for details), and inert to many common solvents.

There is still a number of known incompatibilities with chemicals such as chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %, sulphuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogenes or aequous halogene solutions, phenol and derivatives (cresols, salicylic acid etc.).

When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions normal PEEK capillaries are very sensitive to high pressure. Therefore Agilent uses stainless steel cladded PEEK capillaries in bio-inert systems. The use of stainless steel cladded PEEK capillaries keeps the flow path free of steel and ensures pressure stability to at least 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK.

MS Flow Modulator User Manual 35

4 Using the Module Solvent Information

Polyimide

Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent.

Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like ammonia (e.g. ammonium salts in basic conditions) or acetates.

Polyethylene (PE)

Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow piston and wash seals, which are used in 1290 Infinity pumps, 1290 Infinity II pumps, the G7104C and for normal phase applications in 1260 Infinity pumps.

Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12.5. It is compatible with many organic solvents used in chromatographic systems like methanol, acetonitrile and isopropanol. It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar.

Tantalum (Ta)

Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides.

Stainless Steel (ST) Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by acids below pH 2.3. It can also corrode in following solvents: Solutions of alkali halides, their respective acids (for example, lithium iodide,

potassium chloride, and so on) and aqueous solutions of halogens. High concentrations of inorganic acids like nitric acid, sulfuric acid and

organic solvents especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel).

MS Flow Modulator User Manual 36

4 Using the Module Solvent Information

Halogenated solvents or mixtures which form radicals and/or acids, for example: 2 CHCl3 + O2 2 COCl2 + 2 HCl

This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.

Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, diisopropylether). Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides.

Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel.

Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid).

Mixtures of carbon tetrachloride with 2-propanol or THF.

Titanium (Ti)

Titanium is highly resistant to oxidizing acids (for example, nitric, perchloric and hypochlorous acid) over a wide range of concentrations and temperatures. This is due to a thin oxide layer on the surface, which is stabilized by oxidizing compounds. Non-oxidizing acids (for example, hydrochloric, sulfuric and phosphoric acid) can cause slight corrosion, which increases with acid concentration and temperature. For example, the corrosion rate with 3 % HCl (about pH 0.1) at room temperature is about 13 m/year. At room temperature, titanium is resistant to concentrations of about 5 % sulfuric acid (about pH 0.3). Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates. Titanium is sensitive to acidic metal chlorides like FeCl3 or CuCl2. Titanium is subject to corrosion in anhydrous methanol, which can be avoided by adding a small amount of water (about 3 %). Slight corrosion is possible with ammonia > 10 %.

Diamond-Like Carbon (DLC)

Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

MS Flow Modulator User Manual 37

4 Using the Module Solvent Information

Fused silica and Quartz (SiO2)

Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows. It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides. It is corroded by strong bases and should not be used above pH 12 at room temperature. The corrosion of flow cell windows can negatively affect measurement results. For a pH greater than 12, the use of flow cells with sapphire windows is recommended.

Gold

Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia.

Zirconium Oxide (ZrO2)

Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Platinum/Iridium

Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF)

Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons.

TFE/PDD copolymer tubings, which are used in all Agilent degassers except 1322A/G7122A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel. They have limited life time in the presence of Hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let dry out the chamber. For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off.

The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible with the solvent DMF (dimethyl formamide).

MS Flow Modulator User Manual 38

4 Using the Module Solvent Information

Sapphire, Ruby and Al2O3-based ceramics

Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

MS Flow Modulator User Manual 39

4 Using the Module Status Indicator

Status Indicator

Status indicators 1 Idle 2 Run mode 3 Not-ready. Waiting for a specific pre-run condition to be reached or

completed. 4 Error mode - interrupts the analysis and requires attention (for example a leak

or defective internal components). 5 Resident mode (blinking) - for example during update of main firmware. 6 Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start.

Then try a firmware update.

MS Flow Modulator User Manual 40

4 Using the Module Leak and Waste Handling

Leak and Waste Handling

For details on correct installation, see separate installation documentation.

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 C (392 F). Do not use solvents with a boiling point below 56 C (133 F).

Avoid high vapor concentrations. Always keep the temperature in the sample compartment at least 25 K below the boiling point of the solvent used.

Do not operate the instrument in an explosive atmosphere. Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for

example, carbon disulfide). Reduce the volume of substances to the minimum required for the

analysis. Never exceed the maximum permissible volume of solvents (8 L) in the

solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container. Regularly check the filling level of the waste container. The residual free

volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

NOTE For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet.

MS Flow Modulator User Manual 41

4 Using the Module Leak and Waste Handling

Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with

4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid.

MS Flow Modulator User Manual 42

4 Using the Module Configure the Flow Modulator

Configure the Flow Modulator

1 Choose your module from the list of Modules, click on >.

You can now configure your module. 2 Click Autoconfig to configure the module automatically.

OR Click Configure, manually configure the module and click Ok to confirm the configuration.

The module is ready for use.

MS Flow Modulator User Manual 43

4 Using the Module Configure the Flow Modulator

3 To create a method, right click on the dashboard and select Method....

4 Set the required parameters.

The possible Split Modes are calculated automatically by the software.

MS Flow Modulator User Manual 44

4 Using the Module Configure the Flow Modulator

5 Select the Split Mode.

6 Click Ok to save the method.

MS Flow Modulator User Manual 45

5 Troubleshooting and Diagnostics

User Interfaces 47 Agilent Lab Advisor Software 48

Overview about the troubleshooting and diagnostic features.

MS Flow Modulator User Manual 46

5 Troubleshooting and Diagnostics User Interfaces

User Interfaces

Depending on the user interface, the available tests and the screens/reports may vary.

Preferred tool should be Agilent Lab Advisor Software, see Agilent Lab Advisor Software on page 48.

The Agilent OpenLAB ChemStation C.01.03 and above do not include any maintenance/test functions.

Screenshots used within these procedures are based on the Agilent Lab Advisor Software.

MS Flow Modulator User Manual 47

5 Troubleshooting and Diagnostics Agilent Lab Advisor Software

Agilent Lab Advisor Software

The Agilent Lab Advisor Software is a standalone product that can be used with or without a chromatographic data system. Agilent Lab Advisor helps to manage the lab for high-quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status, Early Maintenance Feedback counters (EMF), instrument configuration information, and diagnostic tests. By the push of a button, a detailed diagnostic report can be generated. Upon request, the user can send this report to Agilent for a significantly improved troubleshooting and repair process. The Agilent Lab Advisor software is available in two versions: Lab Advisor Basic Lab Advisor Advanced

Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument.

The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines. In addition, all diagnostic test results, calibration results, and acquired signal data can be uploaded to a shared network folder. The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated. This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems.

The optional Agilent Maintenance Wizard Add-on provides an easy-to-use, step-by-step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Agilent InfinityLab LC Series instrument.

The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files.

MS Flow Modulator User Manual 48

6 Error Information

What Are Error Messages 50 General Error Messages 51 Timeout 51 Shutdown 52 Remote Timeout 53 Lost CAN Partner 53 Leak Sensor Short 54 Leak Sensor Open 54 Leak 55

Module Specific Error Messages 56 Initialization of Valve Failed 56 Valve Switching Failed 57 Valve Tag Violation 57

This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

MS Flow Modulator User Manual 49

6 Error Information What Are Error Messages

What Are Error Messages

Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook.

If an error occurs outside a method run, other modules will not be informed about this error. If it occurs within a method run, all connected modules will get a notification, all LEDs get red and the run will be stopped. Depending on the module type, this stop is implemented differently. For example, for a pump the flow will be stopped for safety reasons. For a detector, the lamp will stay on in order to avoid equilibration time. Depending on the error type, the next run can only be started, if the error has been resolved, for example liquid from a leak has been dried. Errors for presumably single time events can be recovered by switching on the system in the user interface.

Special handling is done in case of a leak. As a leak is a potential safety issue and may have occurred at a different module from where it has been observed, a leak always causes a shutdown of all modules, even outside a method run.

In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface).

MS Flow Modulator User Manual 50

6 Error Information General Error Messages

General Error Messages

General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well.

Timeout Error ID: 0062

The timeout threshold was exceeded.

Probable cause Suggested actions

1 The analysis was completed successfully, and the timeout function switched off the module as requested.

Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required.

2 A not-ready condition was present during a sequence or multiple-injection run for a period longer than the timeout threshold.

Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required.

MS Flow Modulator User Manual 51

6 Error Information General Error Messages

Shutdown Error ID: 0063

An external instrument has generated a shutdown signal on the remote line.

The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.

Probable cause Suggested actions

1 Leak detected in another module with a CAN connection to the system.

Fix the leak in the external instrument before restarting the module.

2 Leak detected in an external instrument with a remote connection to the system.

Fix the leak in the external instrument before restarting the module.

3 Shut-down in an external instrument with a remote connection to the system.

Check external instruments for a shut-down con- dition.

4 The degasser failed to generate sufficient vacuum for solvent degassing.

Check the vacuum degasser for an error condi- tion. Refer to the Service Manual for the degasser or the pump that has the degasser built-in.

MS Flow Modulator User Manual 52

6 Error Information General Error Messages

Remote Timeout Error ID: 0070

A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis. If a not-ready condition is still present on the remote line after one minute the error message is generated.

Lost CAN Partner Error ID: 0071

During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed.

The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.

Probable cause Suggested actions

1 Not-ready condition in one of the instru- ments connected to the remote line.

Ensure the instrument showing the not-ready condition is installed correctly, and is set up cor- rectly for analysis.

2 Defective remote cable. Exchange the remote cable.

3 Defective components in the instrument showing the not-ready condition.

Check the instrument for defects (refer to the instruments documentation).

Probable cause Suggested actions

1 CAN cable disconnected. Ensure all the CAN cables are connected cor- rectly.

Ensure all CAN cables are installed correctly.

2 Defective CAN cable. Exchange the CAN cable.

3 Defective main board in another module. Switch off the system. Restart the system, and determine which module or modules are not rec- ognized by the system.

MS Flow Modulator User Manual 53

6 Error Information General Error Messages

Leak Sensor Short Error ID: 0082

The leak sensor in the module has failed (short circuit).

The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits. If the current increases above the upper limit, the error message is generated.

Leak Sensor Open Error ID: 0083

The leak sensor in the module has failed (open circuit).

The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits. If the current falls outside the lower limit, the error message is generated.

Probable cause Suggested actions

1 Defective leak sensor. Please contact your Agilent service representa- tive.

2 Leak sensor incorrectly routed, being pinched by a metal component.

Please contact your Agilent service representa- tive.

Probable cause Suggested actions

1 Leak sensor not connected to the main board.

Please contact your Agilent service representa- tive.

2 Defective leak sensor. Please contact your Agilent service representa- tive.

3 Leak sensor incorrectly routed, being pinched by a metal component.

Please contact your Agilent service representa- tive.

MS Flow Modulator User Manual 54

6 Error Information General Error Messages

Leak Error ID: 0064

A leak was detected in the module.

The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent. This changes the resistance of the leak sensor which is sensed by the leak-sensor circuit on the main board.

Probable cause Suggested actions

1 Loose fittings. Ensure all fittings are tight.

2 Broken capillary. Exchange defective capillaries.

MS Flow Modulator User Manual 55

6 Error Information Module Specific Error Messages

Module Specific Error Messages

Initialization of Valve Failed Error ID: 24000

During the initialization process the motor of the valve drive moves to some special positions depending on the installed valve head. A failure in this process means either that the movement couldnt be performed properly or it was not noticed correctly by the sensor.

Probable cause Suggested actions

1 Mechanical problems. Friction too high or blockages on the valve drives motor or on the valve head.

Check valve head for correct installation

Try to identify the source of trouble by install- ing a different valve head if possible.

Contact your Agilent Service representative.

2 Defect Sensor on the Valve Drive Motor Check valve head for correct installation

Try to identify the source of trouble by install- ing a different valve head if possible.

Contact your Agilent Service representative.

MS Flow Modulator User Manual 56

6 Error Information Module Specific Error Messages

Valve Switching Failed Error ID: 24001

The valve drive was not able to operate the valve head correctly. Either due to mechanical reasons or the movement couldnt be detected correctly.

Valve Tag Violation Error ID: 24006

The valve drive identified a different valve head than it had identified during the last initialization.

Probable cause Suggested actions

1 Mechanical problems. Friction too high or blockages on the valve drives motor or on the valve head.

Check valve head for correct installation

Try to identify the source of trouble by install- ing a different valve head if possible.

Contact your Agilent Service representative.

2 Defect Sensor on the Valve Drive Motor Check valve head for correct installation

Try to identify the source of trouble by install- ing a different valve head if possible.

Contact your Agilent Service representative.

Probable cause Suggested actions

1 A valve head has been exchanged (hot-plugged) while the valve drive was still powered on.

Change the valve head. It is important to have the valve switched off for at least 10 s after or before a new valve head has been installed.

NOTE Soft power-down power supply of the valve drive.

Whenever you want to power cycle the valve drive for a re-boot, it needs to be powered off for at least 10 seconds.

MS Flow Modulator User Manual 57

7 Maintenance

Introduction to Maintenance 59 Warnings and Cautions 60 Cleaning the Module 61 Replacing the Fuses of the Infinity Valve Drive 62 Replace Rotor and Stator 64 Replace Valve Heads 67 Replacing the Module Firmware 71 Module Specific Information 72

This chapter describes the maintenance of the module.

MS Flow Modulator User Manual 58

7 Maintenance Introduction to Maintenance

Introduction to Maintenance

The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system.

NOTE There are no serviceable parts inside.

Do not open the module.

MS Flow Modulator User Manual 59

7 Maintenance Warnings and Cautions

Warnings and Cautions

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

The volume of substances should be reduced to the minimum required for the analysis.

Do not operate the instrument in an explosive atmosphere.

WARNING Electrical shock

Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened.

Do not remove the cover of the module.

Only certified persons are authorized to carry out repairs inside the module.

WARNING Personal injury or damage to the product

Agilent is not responsible for any damages caused, in whole or in part, by improper use of the products, unauthorized alterations, adjustments or modifications to the products, failure to comply with procedures in Agilent product user guides, or use of the products in violation of applicable laws, rules or regulations.

Use your Agilent products only in the manner described in the Agilent product user guides.

CAUTION Safety standards for external equipment

If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.

MS Flow Modulator User Manual 60

7 Maintenance Cleaning the Module

Cleaning the Module

To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent.

WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module

Do not use an excessively damp cloth during cleaning.

Drain all solvent lines before opening any connections in the flow path.

MS Flow Modulator User Manual 61

7 Maintenance Replacing the Fuses of the Infinity Valve Drive

Replacing the Fuses of the Infinity Valve Drive

When If the flow module shows no reaction.

Tools required Description Screwdriver

Parts required # p/n Description 2 2110-1486 Fuse 2 AT250 V

WARNING Electrical shock

Disconnect the module from line power before changing a fuse or trying to open the hatch of the power input socket.

Never reconnect the line power before havingthe power input socket closed.

1 Switch off the instrument. The line switch is located at the rear of the flow modulator.

2 Disconnect the power cable from the power input socket.

MS Flow Modulator User Manual 62

7 Maintenance Replacing the Fuses of the Infinity Valve Drive

3 To access the fuse drawer, gently lift the outer plastic housing of the power inlet socket using a flat screw- driver.

4 Pull out the fuse drawer as shown.

5 Replace the defect fuse(s). 6 Slide in the fuse drawer and push till it fits tightly.

7 Finally, close the fuse drawer housing, reconnect the instrument to the power line and switch it on.

MS Flow Modulator User Manual 63

7 Maintenance Replace Rotor and Stator

Replace Rotor and Stator

Parts required p/n Description 1535-5215 RheBuilt kit

NOTE Always replace rotor and stator as pair.

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

Be sure that no solvent can drop out of the solvent connections when removing them from your valve head.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

1 Switch off the module. 2 Remove all capillaries.

MS Flow Modulator User Manual 64

7 Maintenance Replace Rotor and Stator

3 With the hex key remove the screws from the stator. 4 Remove the stator and stator seal from the stator ring (the stator seal usually remains on the stator).

5 Remove the stator ring. 6 Remove the rotor seal from the valve body. The rotor seal is mounted on three pins and can be pulled off.

7 Use clean, filtered compressed gas to blow out the shaft assembly area of the valve

8 Mount the new rotor seal with the slots facing the sta- tor. The three pins on the shaft assembly fit into the mating holes in the Rotor Seal only one way.

MS Flow Modulator User Manual 65

7 Maintenance Replace Rotor and Stator

9 Replace the stator ring so the two short pins enter the include three sets of replacement mating holes in the body.

10 Mount the new stator seal onto the stator. The pins on the assembly fit into the mating holes in the stator only one way.

11 Install the stator. Secure the stator with the screws.

NOTE Do not over-tighten the screws. The screws hold the assem- bly together and do not affect the sealing force. The sealing force is automatically set as the screws close the stator head against the valve body.

12 Reconnect the capillaries and tubes to the valve ports.

NOTE Use the supplied tool to tighten the capillaries.

13 Switch on the module.

MS Flow Modulator User Manual 66

7 Maintenance Replace Valve Heads

Replace Valve Heads

Parts required p/n Description 5067-4292 InfinityLab MS Flow Modulator Valve 100 mL

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

Be sure that no solvent can drop out of the solvent connections when removing them from your valve head.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

CAUTION The valve actuator contains sensitive optical parts, which need to be protected from dust and other pollution. Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results.

Always install a valve head for operation and storage. For protecting the actuator, a dummy valve head (part of Transportation Lock Kit (G1316-67001 )) can be used instead of a functional valve. Do not touch parts inside the actuator.

NOTE The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module. Valve properties will not be updated, if the valve head is replaced while the module is on.

Selection of valve port positions can fail, if the instrument does not know the properties of the installed valve.

NOTE To have the valve correctly recognized by the module you must have the module powered off for at least 10 seconds.

NOTE When there is any unusual behavior, the rotor and stator seals will need to be cleaned, or replaced. You can clean these seals by wiping with a tissue, and then sonicating in isopropanol.

MS Flow Modulator User Manual 67

7 Maintenance Replace Valve Heads

1 Switch off the module. 2 Remove all capillary connections from the valve head

NOTE Use the provided Valve Removal tool if necessary.

MS Flow Modulator User Manual 68

7 Maintenance Replace Valve Heads

3 Unscrew the valve head. 4 Insert the valve head into the valve shaft.

OR If the outside pin does not fit into the outside groove, you have to turn the valve head until you feel that the two pins snap into the grooves. Now you should feel additional resis- tance from the valve drive while continuously turning the valve head until the pin fits into the groove.

MS Flow Modulator User Manual 69

7 Maintenance Replace Valve Heads

5 When the outer pin is locked into the groove, manually screw the nut onto the valve head.

NOTE Fasten the nut with the 5043-1767 Valve Removal tool.

6 Install all required capillary connections to the valve.

7 Power on or power-cycle your module, so the valve head gets recognized during module initialization.

MS Flow Modulator User Manual 70

7 Maintenance Replacing the Module Firmware

Replacing the Module Firmware

To upgrade/downgrade the modules firmware carry out the following steps: 1 Download the required module firmware, the latest FW Update Tool and the

documentation from the Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761

2 For loading the firmware into the module follow the instructions in the documentation.

Module Specific Information

Module is a hosted module and always needs to be connected to a host with matching firmware of same revision.

When The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same (validated) revision. The installation of older firmware might be necessary to keep all systems on the same (validated) revision or if a new module with newer firmware is added to a system or if third party control software requires a special version.

Tools required Description Agilent Lab Advisor software

Parts required # Description 1 Firmware, tools and documentation from Agilent web site

Preparations Read update documentation provided with the Firmware Update Tool.

MS Flow Modulator User Manual 71

7 Maintenance Replacing the Module Firmware

Module Specific Information Table 3 Module Specific Information (G7170B)

Firmware Set B/C/D.07.20

Agilent OpenLab CDS A.02.02/C.01.07 SR3 HF1

LC drivers A.02.17

LabAdvisor B.02.10

MS Flow Modulator User Manual 72

8 Parts and Materials

Accessory Kit for the Flow Modulator 74 Optional Parts 76

This chapter provides information on parts for maintenance.

MS Flow Modulator User Manual 73

8 Parts and Materials Accessory Kit for the Flow Modulator

Accessory Kit for the Flow Modulator

Accessory Kit (G7170-68705)

Item # p/n Description

1 1 5043-0915 Fitting mounting tool

2 1 5043-1767 Valve Removal tool

3 1 5067-6821 Capillary ST, 0.12x100 SX/SX

4 1 5067-6820 Capillary ST, 0.25x80 SX/RLO

5 1 5067-5685 Clamp Guide Kit-IF-II

6 2 2110-1486 Fuse 2 AT250 V

7 1 5181-1519 CAN cable, Agilent module to module, 1 m

8 1 5067-4792 Leak sensor assembly

9 1 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste)

10 1 5500-1156 T-Tube Connector ID6.4

11 1 5043-0270 Leak plane

12 1 5043-0271 Holder leak plane

1 0100-1818 TEE, LOW DEAD VOLUME (not shown)

MS Flow Modulator User Manual 74

8 Parts and Materials Accessory Kit for the Flow Modulator

2 3

5 6

7

9

10

12

11

1

4

8

MS Flow Modulator User Manual 75

8 Parts and Materials Optional Parts

Optional Parts

p/n Description

5067-4292 InfinityLab MS Flow Modulator Valve 100 mL

1535-5215 Active Splitter Rebuild Kit (3 Stator/Rotor pairs)

1535-4045 Bearing ring

1535-4857 Stator screws

5067-1510 Rail assy for column organizer

5067-4634 Valve rail assembly for G7159B

G1369-60012 LAN Interface Card

G9321-60013 Rail assembly for Prep-Scale Fraction Collector

MS Flow Modulator User Manual 76

9 Identifying Cables

Cable Overview 78 Analog Cables 80 Remote Cables 82 CAN/LAN Cables 86 Agilent Module to PC 87 USB 88

This chapter provides information on cables used with the modules.

MS Flow Modulator User Manual 77

9 Identifying Cables Cable Overview

Cable Overview

Analog cables

Remote cables

CAN cables

NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

p/n Description

35900-60750 Agilent 35900A A/D converter

01046-60105 Analog cable (BNC to general purpose, spade lugs)

p/n Description

5188-8029 ERI to general purpose

5188-8044 Remote Cable ERI ERI

5188-8045 Remote Cable APG ERI

5188-8059 ERI-Extension-Cable 1.2 m

5061-3378 Remote Cable to 35900 A/D converter

01046-60201 Agilent module to general purpose

5188-8057 Fraction Collection ERI remote Y-cable

p/n Description

5181-1516 CAN cable, Agilent module to module, 0.5 m

5181-1519 CAN cable, Agilent module to module, 1 m

MS Flow Modulator User Manual 78

9 Identifying Cables Cable Overview

LAN cables

RS-232 cables (not for

FUSION board)

USB cables

p/n Description

5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connec- tion)

p/n Description

RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561 RS-232 cable, 8 m

p/n Description

5188-8050 USB A M-USB Mini B 3 m (PC-Module)

5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive)

MS Flow Modulator User Manual 79

9 Identifying Cables Analog Cables

Analog Cables

One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made.

Agilent Module to 35900 A/D converters

p/n 35900-60750 35900 Pin Agilent module

Signal Name

1 Not connected

2 Shield Analog -

3 Center Analog +

MS Flow Modulator User Manual 80

9 Identifying Cables Analog Cables

Agilent Module to BNC Connector

Agilent Module to General Purpose

p/n 8120-1840 Pin BNC Pin Agilent module

Signal Name

Shield Shield Analog -

Center Center Analog +

p/n 01046-60105 Pin Pin Agilent module

Signal Name

1 Not connected

2 Black Analog -

3 Red Analog +

MS Flow Modulator User Manual 81

9 Identifying Cables Remote Cables

Remote Cables

ERI (Enhanced Remote Interface) 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) 5188-8044 ERI to ERI (D_Sub 15 pin male - male) 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female)

p/n 5188-8029 pin Color code Enhanced Remote

Classic Remote

Active (TTL)

1 white IO1 START REQUEST

Low

2 brown IO2 STOP Low

3 green IO3 READY High

4 yellow IO4 POWER ON High

5 grey IO5 NOT USED

6 pink IO6 SHUT DOWN Low

7 blue IO7 START Low

8 red IO8 PREPARE Low

9 black 1wire DATA

10 violet DGND

11 grey-pink +5V ERI out

12 red-blue PGND

13 white-green PGND

14 brown-green +24V ERI out

15 white-yellow +24V ERI out

NC yellow-brown

MS Flow Modulator User Manual 82

9 Identifying Cables Remote Cables

5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG))

p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL)

10 GND 1

1 Start Request 9 Low

2 Stop 8 Low

3 Ready 7 High

5 Power on 6 High

4 Future 5

6 Shut Down 4 Low

7 Start 3 Low

8 Prepare 2 Low

Ground Cable Shielding NC

MS Flow Modulator User Manual 83

9 Identifying Cables Remote Cables

5188-8057 ERI to APG and RJ45 (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG), Connector plug Cat5e (RJ45))

One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.

Table 4 5188-8057 ERI to APG and RJ45

p/n 5188-8057 Pin (ERI) Signal Pin (APG) Active (TTL) Pin (RJ45)

10 GND 1 5

1 Start Request

9 High

2 Stop 8 High

3 Ready 7 High

4 Fraction Trig- ger

5 High 4

5 Power on 6 High

6 Shut Down 4 High

7 Start 3 High

8 Prepare 2 High

Ground Cable Shield- ing

NC

MS Flow Modulator User Manual 84

9 Identifying Cables Remote Cables

Agilent Module to Agilent 35900 A/D Converters

Agilent Module to General Purpose

p/n 5061-3378 Pin 35900 A/D Pin Agilent module

Signal Name Active (TTL)

1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low

3 - Gray 3 - Gray Start Low

4 - Blue 4 - Blue Shut down Low

5 - Pink 5 - Pink Not connected

6 - Yellow 6 - Yellow Power on High

7 - Red 7 - Red Ready High

8 - Green 8 - Green Stop Low

9 - Black 9 - Black Start request Low

p/n 01046-60201 Wire Color Pin Agilent module

Signal Name Active (TTL)

White 1 Digital ground

Brown 2 Prepare run Low

Gray 3 Start Low

Blue 4 Shut down Low

Pink 5 Not connected

Yellow 6 Power on High

Red 7 Ready High

Green 8 Stop Low

Black 9 Start request Low

MS Flow Modulator User Manual 85

9 Identifying Cables CAN/LAN Cables

CAN/LAN Cables

Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors.

CAN Cables

LAN Cables

p/n Description

5181-1516 CAN cable, Agilent module to module, 0.5 m

5181-1519 CAN cable, Agilent module to module, 1 m

p/n Description

5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connec- tion)

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9 Identifying Cables Agilent Module to PC

Agilent Module to PC

p/n Description

RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561 RS-232 cable, 8 m

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9 Identifying Cables USB

USB

To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket.

p/n Description

5188-8050 USB A M-USB Mini B 3 m (PC-Module)

5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive)

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10 Hardware Information

Electrical Connections 90 Serial Number Information (ALL) 90 Front and Rear View of the Module 91

Interfaces 92 Overview Interfaces 94

Config Switch Settings of the Infinity Valve Drive 98

This chapter describes the module in more detail on hardware and electronics.

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10 Hardware Information Electrical Connections

Electrical Connections

The CAN bus is a serial bus with high speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization.

The power input socket accepts a line voltage of 100 240 VAC 10 % with a line frequency of 50 or 60 Hz. Maximum power consumption varies by module. There is no voltage selector on your module because the power supply has wide-ranging capability. There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.

Serial Number Information (ALL) The serial number information on the instrument labels provide the following information:

NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

CCXZZ00000 Format

CC Country of manufacturing DE = Germany JP = Japan CN = China

X Alphabetic character A-Z (used by manufacturing)

ZZ Alpha-numeric code 0-9, A-Z, where each combination unambig- uously denotes a module (there can be more than one code for the same module)

00000 Serial number

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10 Hardware Information Electrical Connections

Front and Rear View of the Module

Figure 3 Front view of the flow modulator

Figure 4 Rear view of the flow modulator

Status indicator

Valve

Serial number label

Fuses

External leak sensor

connector

Configuration switch

CAN ports

Power switch

Power connector

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10 Hardware Information Interfaces

Interfaces

The Agilent InfinityLab LC Series modules provide the following interfaces:

Table 5 Agilent InfinityLab LC Series Interfaces

Module CAN USB LAN (on-board)

RS-232 Analog APG (A) / ERI (E)

Special

Pumps

G7104A/C 2 No Yes Yes 1 A

G7110B 2 Yes Yes No No E

G7111A/B, G5654A 2 Yes Yes No No E

G7112B 2 Yes Yes No No E

G7120A 2 No Yes Yes 1 A

G7161A/B 2 Yes Yes No No E

Samplers

G7129A/B/C 2 Yes Yes No No E

G7167B/C, G5667A 2 Yes Yes No No E

G7157A 2 Yes Yes No No E

Detectors

G7114A/B 2 Yes Yes No 1 E

G7115A 2 Yes Yes No 1 E

G7117A/B/C 2 Yes Yes No 1 E

G7121A/B 2 Yes Yes No 1 E

G7162A/B 2 Yes Yes No 1 E

G7165A 2 Yes Yes No 1 E

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10 Hardware Information Interfaces

CAN connectors as interface to other modules LAN connector as interface to the control software RS-232C as interface to a computer USB (Universal Series Bus) as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector(s) for signal output

Fraction Collectors

G7158B 2 Yes Yes No No E

G7159B 2 Yes Yes No No E

G7166A 2 No No No No No Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with addi- tional G1369C LAN Card

G1364E/F, G5664B 2 Yes Yes No No E THERMOSTAT for G1330B

Others

G7116A/B 2 No No No No No Requires a HOST mod- ule via CAN

G7122A No No No Yes No A

G7170B 2 No No No No No Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with addi- tional G1369C LAN Card

Table 5 Agilent InfinityLab LC Series Interfaces

Module CAN USB LAN (on-board)

RS-232 Analog APG (A) / ERI (E)

Special

NOTE The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via LAN. The inter-module communication is done via CAN.

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10 Hardware Information Interfaces

Overview Interfaces

CAN

The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement.

LAN

The modules have either an interface slot for a LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD). This interface allows the control of the module/system via a PC with the appropriate control software. Some modules have neither on-board LAN nor an interface slot for a LAN card (e.g. G1170A Valve Drive or G4227A Flexible Cube). These are hosted modules and require a Host module with firmware B.06.40 or later or with additional G1369C LAN Card.

RS-232C (Serial)

The RS-232C connector is used to control the module from a computer through RS-232C connection, using the appropriate software. This connector can be configured with the configuration switch module at the rear of the module. Refer to Communication Settings for RS-232C.

The RS-232C is designed as DCE (data communication equipment) with a 9-pin male SUB-D type connector. The pins are defined as:

NOTE If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN should be connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent detector is part of the system, the LAN interface should be installed in the pump or autosampler.

NOTE There is no configuration possible on main boards with on-board LAN. These are pre-configured for

19200 baud,

8 data bit with no parity and

one start bit and one stop bit are always used (not selectable).

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10 Hardware Information Interfaces

Figure 5 RS-232 Cable

Analog Signal Output

The analog signal output can be distributed to a recording device. For details refer to the description of the modules main board.

Table 6 RS-232C Connection Table

Pin Direction Function

1 In DCD

2 In RxD

3 Out TxD

4 Out DTR

5 Ground

6 In DSR

7 Out RTS

8 In CTS

9 In RI

Instrument PC

Male Female Female Male

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10 Hardware Information Interfaces

APG Remote

The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on.

Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.

The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique). To provide maximum safety within a distributed analysis system, one line is dedicated to SHUT DOWN the systems critical parts in case any module detects a serious problem. To detect whether all participating modules are switched on or properly powered, one line is defined to summarize the POWER ON state of all connected modules. Control of analysis is maintained by signal readiness READY for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal levels are defined as: standard TTL levels (0 V is logic true, + 5.0 V is false), fan-out is 10, input load is 2.2 kOhm against + 5.0 V, and output are open collector type, inputs/outputs (wired- or technique).

NOTE All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with respect to the ground terminal).

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10 Hardware Information Interfaces

Special Interfaces

There is no special interface for this module.

Table 7 Remote Signal Distribution

Pin Signal Description

1 DGND Digital ground

2 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities.

3 START (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities.

4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump). Receiver is any module capable to reduce safety risk.

5 Not used

6 POWER ON (H) All modules connected to system are switched on. Receiver is any module relying on operation of others.

7 READY (H) System is ready for next analysis. Receiver is any sequence con- troller.

8 STOP (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection). Receiver is any module performing run-time controlled activities.

9 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler.

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10 Hardware Information Config Switch Settings of the Infinity Valve Drive

Config Switch Settings of the Infinity Valve Drive

Configuration Switch Settings

Figure 6 Config Switch Figure 7 Dipswitch 1 and 2

Table 8 Configuration Switch Settings

Dipswitch 1 Dipswitch 2 Function

ON (1) ON (1) Default/Standard Operation Mode

ON (1) OFF (0) Forced Cold Start

OFF (0) ON (1) Resident Mode

OFF (0) OFF (0) Not Supported

Dipswitch 1

Dipswitch 2

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10 Hardware Information Config Switch Settings of the Infinity Valve Drive

Special Settings

Boot-Resident

Firmware update procedures may require this mode in case of firmware loading errors (main firmware part). If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident mode. It is not operable as a module. It only uses basic functions of the operating system for example, for communication. In this mode the main firmware can be loaded (using update utilities).

Forced Cold Start

A forced cold start can be used to bring the module into a defined mode with default parameter settings.

CAUTION Loss of data

Forced cold start erases all methods and data stored in the non-volatile memory. Exceptions are calibration settings, diagnosis and repair log books which will not be erased.

Save your methods and data before executing a forced cold start.

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11 Appendix

General Safety Information 101 General Safety Information 101 Safety Standards 101 General 101 Before Applying Power 102 Ground the Instrument 102 Ground Solvent Lines 103 Do Not Operate in an Explosive Atmosphere 103 Do Not Remove the Instrument Cover 103 Do Not Modify the Instrument 103 In Case of Damage 104 Solvents 105 Safety Symbols 106

Waste Electrical and Electronic Equipment (WEEE) Directive 108 Radio Interference 109 Sound Emission 110 Agilent Technologies on Internet 111

This chapter provides addition information on safety, legal and web.

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11 Appendix General Safety Information

General Safety Information

General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.

Safety Standards This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards.

General Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions.

WARNING Ensure the proper usage of the equipment.

The protection provided by the equipment may be impaired.

The operator of this instrument is advised to use the equipment in a manner as specified in this manual.

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11 Appendix General Safety Information

Before Applying Power

Ground the Instrument

WARNING Wrong voltage range, frequency or cabling

Personal injury or damage to the instrument

Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Make all connections to the unit before applying power.

NOTE Note the instrument's external markings described under Safety Symbols on page 106.

WARNING Missing electrical ground

Electrical shock

If your product is provided with a grounding type power plug, the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard.

The ground pin must be firmly connected to an electrical ground (safety ground) terminal at the power outlet. Any interruption of the protective (grounding) conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury.

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11 Appendix General Safety Information

Ground Solvent Lines

Do Not Operate in an Explosive Atmosphere

Do Not Remove the Instrument Cover

Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product. Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained.

WARNING Missing electrical ground

Electrical shock, fire, explosion

Never use solvent guiding tubes, capillaries, and fittings other than the ones supplied by Agilent Technologies for use in preparative systems.

WARNING Presence of flammable gases or fumes

Explosion hazard

Do not operate the instrument in the presence of flammable gases or fumes.

WARNING Instrument covers removed

Electrical shock

Do Not Remove the Instrument Cover

Only Agilent authorized personnel are allowed to remove instrument covers. Always disconnect the power cables and any external circuits before removing the instrument cover.

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11 Appendix General Safety Information

In Case of Damage

WARNING Damage to the module

Personal injury (for example electrical shock, intoxication)

Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel.

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11 Appendix General Safety Information

Solvents

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 C (392 F). Do not use solvents with a boiling point below 56 C (133 F).

Avoid high vapor concentrations. Always keep the temperature in the sample compartment at least 25 K below the boiling point of the solvent used.

Do not operate the instrument in an explosive atmosphere.

Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

Reduce the volume of substances to the minimum required for the analysis.

Never exceed the maximum permissible volume of solvents (8 L) in the solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container.

Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

NOTE For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet.

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11 Appendix General Safety Information

Safety Symbols Table 9 Symbols

The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up.

Cooling unit is designed as vapor-compression refrigeration system. Con- tains fluorinated greenhouse gas (refrigerant) according to the Kyoto proto- col. For specifications of refrigerant, charge capacity, carbon dioxide equivalent (CDE), and global warming potential (GWP) see instrument label.

Confirms that a manufactured product complies with all applicable Euro- pean Community directives. The European Declaration of Conformity is available at: http://regulations.corporate.agilent.com/DoC/search.htm

Manufacturing date.

Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position

Pacemaker Magnets could affect the functioning of pacemakers and implanted heart defibrillators. A pacemaker could switch into test mode and cause illness. A heart defibril- lator may stop working. If you wear these devices keep at least 55 mm dis- tance to magnets. Warn others who wear these devices from getting too close to magnets.

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11 Appendix General Safety Information

Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

Indicates a pinching or crushing hazard

Indicates a piercing or cutting hazard.

Table 9 Symbols

WARNING A WARNING

alerts you to situations that could cause physical injury or death.

Do not proceed beyond a warning until you have fully understood and met the indicated conditions.

CAUTION A CAUTION

alerts you to situations that could cause loss of data, or damage of equipment.

Do not proceed beyond a caution until you have fully understood and met the indicated conditions.

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11 Appendix Waste Electrical and Electronic Equipment (WEEE) Directive

Waste Electrical and Electronic Equipment (WEEE) Directive

This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste.

NOTE Do not dispose of in domestic household waste

To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information.

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11 Appendix Radio Interference

Radio Interference

Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations.

Test and Measurement

If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

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11 Appendix Sound Emission

Sound Emission

Manufacturers Declaration

This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991.

This product has a sound pressure emission (at the operator position) < 70 dB. Sound Pressure Lp < 70 dB (A) At Operator Position Normal Operation According to ISO 7779:1988/EN 27779/1991 (Type Test)

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11 Appendix Agilent Technologies on Internet

Agilent Technologies on Internet

For the latest information on products and services visit our worldwide web site on the Internet at:

http://www.agilent.com

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In This Book

This manual contains technical reference information about the Agilent InfinityLab LC Series 1290 Infinity II MS Flow Modulator (G7170B) The manual describes the following: Introduction Site Requirements and Specification Installation Using the Module Troubleshooting and