Contents

Agilent 7693A Liquid Sampler Maintenance Manual V2 PDF

1 of 282
1 of 282

Summary of Content for Agilent 7693A Liquid Sampler Maintenance Manual V2 PDF

Agilent 7693A Automatic Liquid Sampler

Installation, Operation and Maintenance

Agilent Technologies

2 Installation, Operation, and Maintenance

Notices Agilent Technologies, Inc. 2017

No part of this manual may be reproduced in any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Manual Part Number G4513-90010

Edition Sixth edition, June 2017 Fifth edition, November 2010 Fourth edition, June 2010 Third edition, July 2009 Second edition, May 2009 First edition, February 2009

Printed in USA

Agilent Technologies, Inc. 2850 Centerville Road Wilmington, DE 19808-1610 USA

412 800820 3278

Safety Notices

CAUTION

A CAUTION notice denotes a haz- ard. It calls attention to an operat- ing procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated condi- tions are fully understood and met.

Contents

Part 1: Safety and Regulatory Information

1 Safety and Regulatory Information

Installation, Operation, and Mainten

Important Safety Warnings 14

Many internal parts of the instrument carry dangerous voltages 14

Electrostatic discharge is a threat to instrument electronics 14

Safety and Regulatory Certifications 15

EMC Declaration for South Korea 16 Information 16 Symbols 16 Technical and environmental specifications 17 Electromagnetic compatibility 18 Sound Emission Certification for Federal Republic of

Germany 18 Fuses and battery 19

Cleaning 19

Recycling the Product 19

Part 2: Installation

2 Compatibility

Hardware 24

Firmware 24

ance 3

4

G4513A Injector 25

G4514A Sample Tray 25

G4515A Bar Code Reader/Mixer/Heater 25

G4520A Sample Tray with Installed Bar Code Reader/Mixer/Heater 25

G4522A Heating and Cooling Plate 25

G7390A Intuvo 9000 GC Tray Support Assembly Kit 26

G4526A Upgrade Kit for 6890 Series GCs 27

3 Installation

Preparing the GC 30

Preparing the GC Inlet Area 31

Prepare the Intuvo 9000 GC 32

Install the pivot block and stop bracket 32

Prepare the 7890 Series GC and 7820 MSD 40

Prepare the 6890 Series GCs 41

Replace the inlet fan cover 41 Replace the injection port top cover 41

Installing the G4514A Sample Tray 44

Install the G4514A Sample Tray on the 7890 GC and 7820 MSD 45

Install the mounting bracket 45 Prepare the sample tray 46 Install the sample tray 51 Connect the communication cable 53

Install the G4514A Sample Tray on the Intuvo 9000 GC 54

Install tray bracket onto the tray 54 Install the tray on the GC 57

Installing the G4513A Injector 58

Installation, Operation, and Maintenance

Installation, Operation, and Mainten

Install the injector 58 Check your work 71

Choosing the Turret Type 72

Connecting the Cables 73

Intuvo 9000 GC 73 7890 Series GC 74 7820A GC 75 6890N or 6890 Plus GC 76 6890A GC 77 6850 Series GC 78 7820 MSD 79 5975T LTM-GC/MSD 80 Test the connections 80

Parking the Sample Tray 81

Installing the Vial Racks 82

Install the vial rack labels 82 Install the vial racks 83 Remove the vial rack labels 85

Updating the Firmware 87

View the current firmware version 87 Update the firmware 88 6890A and 6890 Plus GCs 88

Configuring the GC and Data System 89

Configure the GC 89 Configure the data system 89

Calibrating the ALS System 90

Making a Trial Run 93

4 Accessories

Installing the G4526A/G4517A ALS Controller (6890A GCs) 96

ance 5

6

Controller site requirements 96 Verify the power configuration 100

Installing the G4526A/G4516A ALS Interface Controller (6890 Plus GCs) 101

Installing the G4515A Bar Code Reader/Mixer/Heater 105

Remove the sample tray from the GC 105 Install the G4515A Bar Code Reader/Mixer/Heater 106

Installing the G4522A Heating and Cooling Plate 116

Tools needed 116 Remove the sample tray from the GC 116 Install the heating and cooling plate 117 Reassemble the sample tray 126 Install the drainage tubing 127 Complete the installation 128 Coolant 128 Water bath and pump specifications 128

Part 3: Operation

5 Introduction to Operation

About your 7693A Automatic Liquid Sampler 134

Components 134 Features 136 Capabilities 139

Fast Injection 140

Sample Carryover 142

Solvent wash 142 Sample wash 142 Sample pump 142 Number and type of washes 142

Installation, Operation, and Maintenance

Installation, Operation, and Mainten

Methods and Sequences 144

The Sampler Cycle 145

6 ALS Configuration

Configuring the Injector 148

7890 Series GC, 7820A GC, and 7820 MSD 148 6890 Series GC 149 6850 Series GC 151 5975T LTM-GC/MSD 152

Configuring the Sample Tray 153

7890 Series GC and 7820 MSD 153 6890 Series GC 155

Configure the ALS on an Intuvo 9000 GC 158

7 ALS Parameters

Setting the Injector Parameters 162

7890 Series GC, 7820A GC, and 7820 MSD 163 Intuvo 9000 GC 165 6890 Series GC 166 6850 Series GC 168 5975T LTM-GC/MSD 168

Setting the Sample Tray Parameters 169

7890 Series GC and 7820 MSD 169 Intuvo 9000 GC 170 6890 Series GC 170

8 Syringes and Needles

Selecting a Syringe 174

Inspecting a Syringe 177

Installing a Syringe 178

ance 7

8

Removing a Syringe 182

Replacing a Syringe Needle 183

9 Vials and Bottles

Preparing a Sample Vial 186

Select a sample vial 186 Select a vial septa 187 Label a sample vial 188 Fill a sample vial 189 Cap a sample vial 190

Preparing Solvent and Waste Bottles 192

Select the bottles 192 Fill the solvent bottles 193 Prepare the waste bottles 193

Placing Vials and Bottles in the Turret 194

Using Two Injectors (7890A and 6890 Series GCs Only) 197

With a sample tray 197 Without a sample tray 197

How Many Sample Vials Can I Run? 198

Solvent bottle equation (injector) 199 Waste bottle equation 199 Example 200 Reduced solvent and sample usage 203

Sandwich Injections 204

Example 2-layer sandwich injection 206 Example 3-layer sandwich injection 208

10 Running Samples

Running a Sample 212

Injection volume 212

Installation, Operation, and Maintenance

Installation, Operation, and Mainten

Using the ALS Controller 213

Interrupting a Run or Sequence 214

Sampler response to interruptions 214 Restarting an interrupted sequence 214

Running a Priority Sample 215

Part 4: Maintenance and Troubleshooting

11 Maintenance

Periodic Maintenance 220

Tray Home and Park Positions 222

Installing a Syringe 223

Removing a Syringe 227

Changing the Turret 228

Adapting for Cool On-Column Injection 232

Replacing the Needle Support Foot 233

Adapting for Syringes Over 100 L 235

Replacing the Syringe Carriage Assembly 236

Replacing a Syringe Needle 243

Aligning the Injector 245

Aligning the Sample Tray 247

Calibrating the ALS System 249

Replacing Power Fuses in the G4517A ALS Controller 252

12 Faults and Errors

Faults 256

Injector faults 256

ance 9

10

Sample tray faults 258

Error Messages 260

13 Troubleshooting

Symptom: Variability 266

Symptom: Contamination or Ghost Peaks 268

Symptom: Smaller or Larger Peaks Than Expected 269

Symptom: Sample Carryover 271

Symptom: No Signal/No Peaks 272

Correcting Syringe Problems 273

Correcting Sample Vial Delivery Problems 274

14 Replacement Parts

G4513A Injector 276

G4514A Sample Tray 278

G4517A ALS Controller (6890A GC Only) 280

G4516A ALS Interface Board (6890 Plus GC Only) 282

Installation, Operation, and Maintenance

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

Part 1:

Safety and Regulatory Information

Important Safety Warnings 14

Safety and Regulatory Certifications 15

Cleaning 19

Recycling the Product 19

11Agilent Technologies

12

Installation, Operation, and Maintenance

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

1 Safety and Regulatory Information

Important Safety Warnings 14

Many internal parts of the instrument carry dangerous voltages 14

Electrostatic discharge is a threat to instrument electronics 14

Safety and Regulatory Certifications 15

Information 16

Symbols 16

Technical and environmental specifications 17

Electromagnetic compatibility 18

Sound Emission Certification for Federal Republic of Germany 18

Fuses and battery 19

Cleaning 19

Recycling the Product 19

This chapter provides important safety and regulatory information about the Agilent 7693A Automatic Liquid Sampler (ALS) system.

13Agilent Technologies

Safety and Regulatory Information

Important Safety Warnings

14

There are several important safety notices that you should always keep in mind when using the ALS (including any injector, tray, or powered accessory).

Many internal parts of the instrument carry dangerous voltages

With the GC power switch on, potentially dangerous voltages can exist on:

All electronics boards in the instrument

The internal wires and cables connected to these boards

If using a G4517A ALS Controller and it is connected to a power source, even if the power switch is off, potentially dangerous voltages exist on:

The wiring between the instrument power cord and the AC power supply

The AC power supply itself

The wiring from the AC power supply to the power switch

The wiring from the G4517A ALS Controller to any injector or sample tray

Electrostatic discharge is a threat to instrument electronics

WARNING All these parts are shielded by covers. With the covers in place, it should be difficult to accidentally make contact with dangerous voltages. Unless specifically instructed to, never remove a cover.

WARNING If the power cord or wiring from the instrument to the gas chromatograph insulation is frayed or worn, the cord must be replaced. Contact your Agilent service representative.

The printed circuit (PC) boards in the instrument can be damaged by electrostatic discharge. Do not touch any of the boards unless it is absolutely necessary. If you must handle them, wear a grounded wrist strap and take other antistatic precautions. Wear a grounded wrist strap any time you must remove the electronics cover.

Installation, Operation, and Maintenance

Safety and Regulatory Information

Safety and Regulatory Certifications

Installation, Operati

The ALS conforms to the following safety standards:

International Electrotechnical Commission (IEC): 610101

EuroNorm (EN): 610101

The instrument conforms to the following regulations on Electromagnetic Compatibility (EMC) and Radio Frequency Interference (RFI):

CISPR 11/EN 55011: Group 1, Class A

IEC/EN 61326-1

AUS/NZ

This ISM device complies with Canadian ICES-001. Cet appareil ISM est conforme a la norme NMB001 du Canada.

The instrument is designed and manufactured under a quality system registered to ISO 9001.

Instructions for Disposal of Waste Equipment by Users in the European Union. This symbol on the product or its packaging indicates that this product must not be disposed of with other waste. Instead, it is your responsibility to dispose of your waste equipment by handing it over to a designated collection point for the recycling of waste electrical and electronic equipment. The separate collection and recycling of your waste equipment at the time of disposal will help conserve natural resources and ensure that it is recycled in a manner that protects human health and the environment. For more information about where you can drop off your waste equipment for recycling, please contact your local city recycling office or the dealer from whom you originally purchased the product.

on, and Maintenance 15

Safety and Regulatory Information

EMC Declaration for South Korea

16

. .

This equipment has been evaluated for its suitability for use in a commercial environment. When used in a domestic environment, there is a risk of radio interference.

Information

The Agilent Technologies ALS meets the following IEC (International Electrotechnical Commission) classifications: Safety Class I, Installation Category, Pollution Degree 2.

This unit has been designed and tested in accordance with recognized safety standards and is designed for use indoors. If the instrument is used in a manner not specified by the manufacturer, the protection provided by the instrument may be impaired. Whenever the safety protection of the Agilent ALS has been compromised, disconnect the unit from all power sources and secure the unit against unintended operation.

Refer servicing to qualified service personnel. Substituting parts or performing any unauthorized modification to the instrument may result in a safety hazard.

Symbols

Warnings in the manual or on the instrument must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions violates safety standards of design and the intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.

Installation, Operation, and Maintenance

Safety and Regulatory Information

Installation, Operati

See accompanying instructions for more information.

Indicates a hot surface.

Indicates hazardous voltages.

Indicates earth (ground) terminal.

Indicates explosion hazard.

Indicates electrostatic discharge hazard.

Technical and environmental specifications

Indoor use only in ordinary atmospheres

Altitude up to 4300 m

Operating ambient temperatures between 5 degrees centigrade and 55 degrees centigrade

Maximum relative humidity of 80 % for temperatures up to 31 degrees centigrade decreasing linearly to 50 % relative humidity at 40 degrees centigrade

Pollution degree 2, Installation Cat II

In addition to the above technical and environmental specifications, the G4517A ALS Controller conforms to the following technical and environmental specifications:

Rated for mains connection to 100120 VAC or 220240 VAC, 50/60 Hz, 180 VA

Main supply voltage fluctuations up to 10% of the nominal voltage

on, and Maintenance 17

Safety and Regulatory Information

Electromagnetic compatibility

18

This device complies with the requirements of CISPR 11 and IEC 61326-1. Operation is subject to the following two conditions:

1 This device may not cause harmful radio frequency interference.

2 This device must accept any radio frequency interference received, including interference that may cause undesired operation.

If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try one or more of the following measures:

1 Relocate the radio or antenna.

2 Move the device away from the radio or television.

3 Plug the device into a different electrical outlet, so that the device and the radio or television are on separate electrical circuits.

4 Make sure that all peripheral devices are also certified.

5 Make sure that appropriate cables are used to connect the device to peripheral equipment.

6 Consult your equipment dealer, Agilent Technologies, or an experienced technician for assistance.

7 Changes or modifications not expressly approved by Agilent Technologies could void the users authority to operate the equipment.

Sound Emission Certification for Federal Republic of Germany

Sound pressure

Sound pressure Lp < 82 dB(A) according to DIN-EN 27779 (Type test).

Schalldruckpegel

Schalldruckpegel LP < 82 dB(A) nach DIN-EN 27779 (Typprufung).

Installation, Operation, and Maintenance

Safety and Regulatory Information

Fuses and battery

Installation, Operati

Table 1 lists replaceable fuses in the G4517A ALS Controller module. Any fuses in the G4516A ALS Interface Controller Board should only be accessed by Agilent service personnel.

There are no fuses in any other 7693A ALS components.

The G4517A ALS Controller also contains a non-replaceable 3 V lithium ion battery.

Table 1 G4517A ALS Controller fuses

Fuse designation Location Fuse rating and type

2A G4517A Power line module 2A 250V, type T (220240 V power supply)

2A G4517A Power line module 2A 250V, type T (100120 V power supply)

Cleaning

To clean the external surfaces of the injector tower and sample tray, disconnect the power and wipe down with a damp, lint-free cloth. Refer to Periodic Maintenance on page 220 for more information.

Recycling the Product

For recycling, contact your local Agilent sales office.

on, and Maintenance 19

20

Safety and Regulatory Information

Installation, Operation, and Maintenance

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

Part 2:

Installation

Compatibility 23

Hardware 24

Firmware 24

G4513A Injector 25

G4514A Sample Tray 25

G4515A Bar Code Reader/Mixer/Heater 25

G4520A Sample Tray with Installed Bar Code Reader/Mixer/Heater 25

G4522A Heating and Cooling Plate 25

G7390A Intuvo 9000 GC Tray Support Assembly Kit 26

Installation 29

Preparing the GC 30

Preparing the GC Inlet Area 31

Installing the G4514A Sample Tray 44

Installing the G4513A Injector 58

Choosing the Turret Type 72

Connecting the Cables 73

Parking the Sample Tray 81

Installing the Vial Racks 82

Updating the Firmware 87

Configuring the GC and Data System 89

Making a Trial Run 93

Accessories 95

Installing the G4526A/G4517A ALS Controller (6890A GCs) 96

Installing the G4526A/G4516A ALS Interface Controller (6890 Plus GCs) 101

Installing the G4515A Bar Code Reader/Mixer/Heater 105

Installing the G4522A Heating and Cooling Plate 116

21Agilent Technologies

22

Installation, Operation, and Maintenance

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

2 Compatibility

Hardware 24

Firmware 24

G4513A Injector 25

G4514A Sample Tray 25

G4515A Bar Code Reader/Mixer/Heater 25

G4520A Sample Tray with Installed Bar Code Reader/Mixer/Heater 25

G4522A Heating and Cooling Plate 25

G7390A Intuvo 9000 GC Tray Support Assembly Kit 26

G4526A Upgrade Kit for 6890 Series GCs 27

This chapter assists you in determining whether the 7693A ALS system is suitable for your Agilent Gas Chromatograph (GC) and aids in identifying existing equipment.

23Agilent Technologies

2 Compatibility

Hardware

24

The 7693A ALS system is compatible with the following Agilent instruments:

Intuvo 9000 GC

7890 Series GC

7820A GC

6890 Series GC

6850 Series GC

7820 MSD

5975T LTM-GC/MSD

Firmware

Agilent GCs require the minimum firmware revisions listed in Table 2.

To update firmware, use the Agilent GC Firmware Update utility, available from the Agilent web site and from the Agilent User Manuals & Tools DVDs.

Table 2 Minimum GC firmware revisions for 7693A ALS system use

Instrument Minimum firmware version required

Intuvo 9000 GC A.01.02

7890 Series GC A.01.10

7820A GC A.01.01

6890N GC N.06.07

6890A GC A.03.08 (chip set)

6890 Plus GC A.03.08 (chip set)

6850 GC with serial number < US00003200 A.03.07

6850 GC with serial number > US10243001 (includes 6850 Network and Series II)

A.06.02

7820 MSD A.01.01 (GC)

5975T LTM-GC/MSD A.03.02.005 (GC) / 5.02.07 (MSD)

Installation, Operation, and Maintenance Part 2, Installation

Compatibility 2

G4513A Injector

Installation, Operati Part 2, Installation

The G4513A Injector is designed for the Agilent 7693A ALS system. No other injector models are compatible.

G4514A Sample Tray

The G4514A Sample Tray is designed for the Agilent 7693A ALS system. No other sample tray models are compatible.

Samples may be handled entirely with the 16-sample standalone turret or the 3-sample transfer turret supplied with the injector. The G4514A Sample Tray has a capacity of 150 samples.

G4515A Bar Code Reader/Mixer/Heater

The G4515A Bar Code Reader/Mixer/Heater (BCR) is designed for the Agilent 7693A ALS system, and provides positive sample identification checks, plus single vial heating and mixing capabilities. No other BCRs, mixers, or heaters are compatible.

G4520A Sample Tray with Installed Bar Code Reader/Mixer/Heater

The G4520A Sample Tray with pre-installed BCR/Mixer/Heater is identical to the individual parts G4514A Sample Tray and G4515A BCR/Mixer/Heater. In this manual it is assumed that any reference to G4514A or G4515A also applies to the G4520A accessory.

G4522A Heating and Cooling Plate

The Agilent G4522A Heating and Cooling Plate is designed specifically for the G4514A Sample Tray. No other cooling accessories are compatible.

on, and Maintenance 25

2 Compatibility

G7390A Intuvo 9000 GC Tray Support Assembly Kit

26

The G7390A Intuvo 9000 7693A Tray Support Assembly Kit is required for an Intuvo 9000 GC with an Agilent 7693A ALS system.

Intuvo 9000 7693A Tray Support Assembly kit contents:

ALS support truss

Pivot block

Shoulder screw (0515-3005)

Tray bracket assembly

Stop bracket

Screws (0515-2113) Screws for support truss (0515-6137)

Pivot block backers

Installation, Operation, and Maintenance Part 2, Installation

Compatibility 2

G4526A Upgrade Kit for 6890 Series GCs

Installation, Operati Part 2, Installation

The G4526A Upgrade Kit is required for any 6890 Series GC with an Agilent 7693A ALS system. The Upgrade Kit includes the following:

G1530-40205 Inlet Fan Cover

G1530-40070 Injection Port Top Cover (tahitian teal)

0515-2496 M4 0.7 12 mm screw (3)

1390-1024 M4 0.7 screw (1)

GC and GC/MS User Manuals & Tools DVD

The following options are available depending on your 6890 GC type:

Option 001-Required for 6890A GCs with the last five serial numbers < 20000. Includes the external G4516-64000 ALS Controller.

Option 002-Required for 6890 Plus GCs with the last five serial numbers > 20000. Includes the G4517-64000 ALS Interface Board controller.

Option 003-Required for all 6890N GCs. Includes the G1530-41205 Inlet Fan Cover and the G1530-40075 Injection Port Top Cover (tahitian teal) for older 6890N GC models, or the G1530-41075 Injection Port Top Cover (light gray) for newer 6890N GC models.

NOTE If your 6890A or 6890 Plus GC does not have a LAN connection, the G2335A 6890 LAN kit is also required.

on, and Maintenance 27

28

2 Compatibility

Installation, Operation, and Maintenance Part 2, Installation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

3 Installation

Preparing the GC 30

Preparing the GC Inlet Area 31

Prepare the Intuvo 9000 GC 32

Prepare the 7890 Series GC and 7820 MSD 40

Installing the G4514A Sample Tray 44

Install the G4514A Sample Tray on the 7890 GC and 7820 MSD 45

Install the G4514A Sample Tray on the 7890 GC and 7820 MSD 45

Installing the G4513A Injector 58

Choosing the Turret Type 72

Connecting the Cables 73

Parking the Sample Tray 81

Installing the Vial Racks 82

Updating the Firmware 87

Configuring the GC and Data System 89

Calibrating the ALS System 90

Making a Trial Run 93

The procedure for installing the 7693A ALS depends on the system components purchased and the type of GC involved. In all cases, you must remove any existing ALS system components before installation. Older GCs will require firmware updates. Follow the steps in this chapter that are relevant to your GC and ALS system setup.

29Agilent Technologies

3 Installation

Preparing the GC

30

This procedure explains how to prepare an Agilent GC for the 7693A ALS system.

WARNING The inlet may be hot enough to cause skin burns. Allow the inlet to cool to ambient temperature before working near the inlet.

1 Set the GC inlets, detectors, and oven to room temperature.

2 After the GC inlets, detectors, and oven have cooled, turn off the GC and unplug the power cord.

3 If installed, unplug any ALS component cables. Remove any injectors, injector inlet mounting posts, parking posts, tray brackets and supports, and sample tray from the GC.

For details, refer to the samplers original documentation.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Preparing the GC Inlet Area

Installation, Operati Part 2, Installation

This procedure explains how to prepare the Intuvo 9000 GC, 7890 Series GC, 6890 Series GC, and the 7820 MSD inlet area for the 7693A ALS system.

If you have a 7820A GC, a 6850 Series GC, or the 5975T LTM-GC/MSD, skip this section.

WARNING The inlet may be hot enough to cause skin burns. Allow the inlet to cool to ambient temperature before working near the inlet.

on, and Maintenance 31

3 Installation

Prepare the Intuvo 9000 GC

32

If installing a 7693A ALS tray with an Intuvo 9000 GC, install the pivot bracket, support bracket, and support truss now. (Refer to the Intuvo 9000 7693A Tray Support Assembly, accessory G7390A).

Install the pivot block and stop bracket

Install pivot block 1 Remove the GC top cover.

5 mmT20

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

2 Remove the GC right side panel.

3 Remove left side panel.

on, and Maintenance 33

34

3 Installation

4 Install pivot block with shoulder screw (0515-3005).

5 Install the two pivot block backers (G4580-00272) with two screws (0515-2113), then attach three screws (0515-2113) to the bottom three holes of the pivot block.

0515-3005

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

6 If not using a D2 or MSD, remove the two captive screws in the left-side panel. Install the left side panel onto the GC (without screws you will install two new screws in a later step). Install the MSD frame cover.

7 If using a D2 or MSD, install the left side panel onto the GC. (Do not install the MSD frame cover.)

on, and Maintenance 35

36

3 Installation

8 If not using a D2 or MSD, install the MSD cover insert, then attach the support truss (G4580-60517) to left side of GC.

If using a D2 configuration or MSD, you will not have a support truss. Skip this step.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

a Match the slots on the left side of the GC.

b Secure the support truss with two thumbscrews (0515-6137).

on, and Maintenance 37

38

3 Installation

Install the stop bracket on the right side of the GC 1 Install the stop bracket with four screws (0515-2113). The label

ONE DEGREE TILT should face outward.

ONE DEGREE TILT

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

2 Install the right-side cover.

3 Reinstall the GC top cover.

on, and Maintenance 39

3 Installation

Prepare the 7890 Series GC and 7820 MSD

40

The 7890 Series GC and 7820 MSD inlet area (injection port top cover, inlet fan cover) is designed to be fully-compatible with the 7693A ALS system, but must be prepared if installing a sample tray. If not installing a sample tray, skip this section.

This section contains instructions and photos for the 7890 Series GC. Steps for the 7820 MSD are similar.

Remove five of the seven screws securing the inlet cover to the GC. Do not remove the two screws in the locations shown below.

Do not remove the screws in these locations

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Prepare the 6890 Series GCs

Installation, Operati Part 2, Installation

All 6890 Series GCs require the replacement of the inlet fan cover before installing any component of the 7693A ALS system. Most 6890 Series GCs also require the replacement of the injection port top cover. The procedure below prepares the 6890 Series GC inlet area for the 7693A ALS system.

Replace the inlet fan cover

The inlet fan cover encloses the fan that draws air through the 6890 GC inlets.

1 Loosen the Torx T-20 screw on the right side of the fan cover. See Figure 2 on page 42 for screw location.

2 Slide the cover slightly to the right to disengage it from the left mounting post and lift the cover up and off.

3 Lower the replacement inlet fan cover (G1530-41205) and engage it to the left mounting post.

4 Install the Torx T-20 screw on the right side of the fan cover until tight.

Replace the injection port top cover

The injection port top cover is the plastic cover mounted over the two inlets. In most cases, you will need to replace the injection port top cover before using the 7693A ALS system. However, some later-model 6890 Series GCs were manufactured with a compatible injection port top cover. If your injection port top cover has the two features noted in Figure 1, then you can skip this section. Otherwise follow the procedure below.

on, and Maintenance 41

42

3 Installation

1 Fully loosen the six Torx T-20 screws on the top of the cover (Figure 2).

Figure 1 Compatible injection port top cover features

Figure 2 Removing the injection port top cover and inlet fan cover (6890 Series GC)

Inlet cover screws

Fan cover screw

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

2 Lift off the cover.

3 Set the replacement inlet cover (G1530-41075) on the GC. Make sure all plumbing and wires are properly routed in their channels.

4 Secure the replacement injection port top cover using two screws from the original inlet cover in the hole locations (Figure 3).

5 If installing a sample tray, cover replacement is complete. Be sure to store the remaining screws in a safe location. Skip to the next section.

If not installing a sample tray, install the remaining four Torx T-20 screws on the injection port top cover.

Figure 3 Replacing the injection port top cover and inlet fan cover (6890 Series GC)

Reuse screws from original cover

on, and Maintenance 43

3 Installation

Installing the G4514A Sample Tray

44

This procedure explains how to install the G4514A Sample Tray on the Intuvo 9000 GC, 7890 Series GC, 6890 Series GC, and 7820 MSD.

If you have a 7820A GC, a 6850 Series GC, a 5975T LTM-GC/MSD, or are not planning to install a sample tray, skip this section.

If you have a 6890A GC, be sure to install the G4526A/G4517A ALS Controller before installing the G4514A Sample Tray. See Installing the G4526A/G4517A ALS Controller for more information.

If you have a 6890 Plus GC, be sure to install the G4526A/G4516A ALS Interface Controller before installing the G4514A Sample Tray. See Installing the G4526A/G4516A ALS Interface Controller for more information.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Install the G4514A Sample Tray on the 7890 GC and 7820 MSD

Install the mounting bracket

Installation, Operati Part 2, Installation

1 Attach the mounting bracket (G4514-63000) to the inlet cover.

1390-1024 Screw M4

G4514-20529 Washer

0515-0437 Screw M4

on, and Maintenance 45

3 Installation

Prepare the sample tray

46

WARNING Take care when holding the tray. Because the motors are heavy and located off-center, an improper balance point can cause the tray to tip out of your grasp.

1 Set the tray on a secure, flat surface. Position the tray on its side so the tray bracket faces up. Let the gantry slide all the way down.

2 Slide the gripper jaw away from the tray base until the packing screw is accessible.

Expose packing screw

Slide out

Slide down

Gripper jaw

Gantry

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

3 Remove the packing screw using a Phillips screw driver and tweezers.

Packing screw

on, and Maintenance 47

48

3 Installation

4

5

T-20

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

6

7

T-20

on, and Maintenance 49

50

3 Installation

8

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Install the sample tray

Installation, Operati Part 2, Installation

1 Using both hands, carefully hang the sample tray bracket over the mounting bracket tabs (Figure 4). Try to keep the gantry from sliding towards the tray bracket while performing this step.

2 Tilt the tray, line up the tray bracket with the tabs on the mounting bracket, and slowly lower into place.

Figure 4 Hanging the tray bracket over the mounting bracket tabs.

on, and Maintenance 51

52

3 Installation

3 Once attached, make sure that the sample tray is lying flat against the mounting bracket. Each tab on the mounting bracket must be fully latched to the tray bracket (Figure 5).

Figure 5 Sample tray correctly installed.

Figure 6 Sample tray incorrectly installed.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

4 Secure the sample tray to the mounting bracket by installing three T-30 Torx screws.

Connect the communication cable

1 Connect the communication cable to the sample tray. See Connecting the Cables on page 73 for more information.

on, and Maintenance 53

3 Installation

Install the G4514A Sample Tray on the Intuvo 9000 GC

54

If a tray is not available, skip this section.

Install tray bracket onto the tray

1 Prepare the tray. See Prepare the sample tray on page 46.

2 Park gently.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

3 Place the bracket on the tray. Tighten the captive screws.

Figure 7 Assembling the tray bracket to the tray

on, and Maintenance 55

56

3 Installation

Figure 8 Bracket and tray (assembled)

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Install the tray on the GC

Installation, Operati Part 2, Installation

1 Place tray on the GC, inserting the tray bracket into the stop bracket.

2 Lock the tray in place.

3 Connect the tray to the GC (the ALS 2 connector).

on, and Maintenance 57

3 Installation

Installing the G4513A Injector

Install the injector

58

This procedure explains how to install the G4513A Injector.

If installing the G4514A Sample Tray with your ALS system, you must install the mounting bracket first. See Install the mounting bracket on page 45 for more information.

If installing the G4514A Sample Tray with your ALS system on an Intuvo 9000 GC, you must install the mounting bracket first. See Install the G4514A Sample Tray on the Intuvo 9000 GC on page 54 for more information.

If you have a 6890A GC, be sure to install the G4526A/G4517A ALS Controller before installing the G4514A Sample Tray. See Installing the G4526A/G4517A ALS Controller for more information.

If you have a 6890 Plus GC, be sure to install the G4526A/G4516A ALS Interface Controller before installing the G4514A Sample Tray. See Installing the G4526A/G4516A ALS Interface Controller for more information.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

1 7890 Series GC. Install the parking post(s) on the GC. If you have a 5975T LTM-GC/MSD, skip to the next step.

Figure 9 Parking post positions (7890A shown)

Parking post positions

on, and Maintenance 59

60

3 Installation

Intuvo 9000 GC.

2 Lay the injector on a flat surface, or mount the injector on a parking post.

3 Remove the shipping tape from the turret and injector tower door.

4 Open the injector door.

Figure 10 Installing the parking post on an Intuvo 9000 GC

Parking post

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

5 Using a T-10 Torx driver, completely loosen the T-10 screw and remove the shipping clamp from the syringe carriage (Figure 11 and Figure 12).

Figure 11 Shipping clamp installed.

Shipping clamp

on, and Maintenance 61

62

3 Installation

6 Close the injector door.

Figure 12 Shipping clamp removed.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

CAUTION In the following steps, use a flat blade screwdriver that snugly fits the slot in the top of the posts. An undersized blade can damage the post top and prevent the injector from mounting properly.

Do not mount the G4513A Injector on a mounting post for any other injector; this can

CAUTION damage the injector. Remove the old post and replace it with the new one.

7 Install the injector mounting post (G4513-20561, Figure 13) to the GC inlet covers threaded connection. If the wrong post is used (for example the 7683B ALS mounting post) the injector will not work. The mounting post supplied in this kit only supports the G4513A Injector.

Figure 13 Mounting post (G4513-20561)

on, and Maintenance 63

64

3 Installation

Figure 14 Intuvo 9000 GC. Install the mounting post.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

7890 Series GC, 7820A GC, and 7820 MSD. Install into the inlet cover in the front or rear location, as desired. The post must be turned all the way down (Figure 15).

Figure 15 Installing the mounting post(s) on a 7890 Series GC

Mounting posts

Front injector position Back injector position

on, and Maintenance 65

66

3 Installation

6890 Series GC. Install into the inlet cover in the front or rear location, as desired. The post must be turned all the way down (Figure 16).

6850 Series GC. Install into the inlet mounting bracket. Loosen the inlet screws, install the post and tighten the screws. The post must be turned all the way down (Figure 17).

Figure 16 Installing the mounting post(s) (6890 GCs)

Figure 17 Installing the mounting post (6850 GCs)

6890

Front

Back

6850

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

5975T LTM-GC/MSD. Install into the inlet mounting bracket. The post must be turned all the way down (Figure 18).

Figure 18 Installing the mounting post (5975T LTM-GC/MSD)

5975T LTM-GC/MSD

on, and Maintenance 67

68

3 Installation

8 Intuvo 9000 GC. Mount the injector , then skip to Check your work on page 71.

Figure 19 Mount the injector on the Intuvo 9000 GC.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

9 If a sample tray is installed, route the front injector cable through the mounting bracket support structure. If installing a back injector, make sure the cable is available.

10 If installing a back injector, connect the back injector cable to the injector cable port.

Figure 20 Front injector cable routed through mounting bracket support.

Front injector cable routed Back injector cable through mounting bracket support

Front injector cable

Injector cable port

on, and Maintenance 69

70

3 Installation

11 7890 Series GC. Position the back injector on the mounting post and the back inlet covers support foot.

12 If installing a front injector, connect the front injector cable to the injector cable port.

Back injector

Injector cable port

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

13 Position the front injector on the mounting post and the front inlet covers support foot.

Front injector

Check your work

The injector must be vertical and stable.

If the injector will not sit upright on the GC, check that the plumbing and cabling under the inlet cover are properly routed in their channels. Also verify that the front injector cable is routed correctly through the GC mounting bracket support as shown in Figure 20 on page 69.

on, and Maintenance 71

3 Installation

Choosing the Turret Type

72

Two interchangeable sample turrets are provided with the injector:

The standalone turret allows you to analyze up to 16 samples. It holds two solvent positions and one waste bottle position. Two sample positions can be alternately configured for sample preparation. The standalone turret is not compatible with the sample tray.

The transfer turret is designed to work with the sample tray to analyze up to 150 samples. The transfer turret has three sample vial transfer locations; two of which are configurable for use in sample preparation. There are six solvent A positions, four solvent B positions, and five waste positions. This turret can be used with or without the sample tray.

The injector comes with the transfer turret installed. If you wish to use the 16-sample standalone turret, see Changing the Turret for details.

No other turrets are compatible with the G4513A Injector.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Connecting the Cables

Installation, Operati Part 2, Installation

This section shows the cable routing for a 7693A ALS system with a GC.

Intuvo 9000 GC

Connect the cable to the GC (ALS 1) connector.

on, and Maintenance 73

3 Installation

7890 Series GC

74

Follow the instructions below to properly wire the injector and sample tray to the 7890 Series GC.

1 Connect the injector(s) to the GC with a G4514-60610 cable(s).

2 Connect the tray to the GC with a G4514-60610 cable.

3 Connect the GC power cord to the outlet.

Figure 21 Cables for 7890 Series GC

Power cords not shown

Injectors

BackFront

Inj1 Inj2

Cables (G4514-60610)

7890 Series GC

TraySampler1 Sampler2

Tray (Includes optional BCR)

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

7820A GC

Installation, Operati Part 2, Installation

Follow the instructions below to properly wire the injector to the 7820A GC.

1 Connect the injector to the GC with a G4514-60610 cable.

2 Connect the GC power cord to the outlet.

Figure 22 Cables for 7820A GC

Power cords not shown

Front

Cables (G4514-60610)

7820A GC

ALS Port Front

Injector Back

Injector

Back

on, and Maintenance 75

3 Installation

6890N or 6890 Plus GC

76

Follow the instructions below to properly wire the injector and sample tray to a 6890N or 6890 Plus GC.

1 Connect the injector(s) to the GC with a G4514-60610 cable(s).

2 Connect the tray to the GC with a G4514-60610 cable.

3 Connect the GC power cord to the outlet.

Figure 23 Cables for 6890 Plus and 6890N GCs

Power cords not shown

Injectors

BackFront

Inj1 Inj2

6890 Plus or 6890N GC

TraySampler1 Sampler2

Cables (G4514-60610)

Tray (Includes optional BCR)

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

6890A GC

Installation, Operati Part 2, Installation

Follow the instructions below to properly wire the 6890A GC to the G4517A ALS Controller, and the controller to the injector(s) and sample tray. See Installing the G4526A/G4517A ALS Controller for installation instructions for the G4517A ALS Controller including the power supply cord.

1 Connect the injector(s) to the ALS controller with a G4514-60610 cable(s).

2 Connect the sample tray to the ALS controller with a G4514-60610 cable.

3 Connect the ALS controller to the GC with a G1530-60930 cable and a G1530-60600 cable.

4 Connect the GC and controller power cords to the outlets.

Figure 24 Cables for 6890A GC

Power cords not shown

6890A GC

Remote

Sampler Injectors

BackFront

Inj1 Inj2

G4517A Controller

Tray Front Injector

Back InjectorRS-232

G1530-60600

G1530-60930 6890 Remote

Cables (G4514-60610)

Tray

(Includes optional BCR)

on, and Maintenance 77

3 Installation

6850 Series GC

78

Follow the instructions below to properly wire the 6850 Series GC to the to the injector.

1 Connect the injector(s) to the controller with a G4514-60610 cable. Use the top injector connection on the back of the GC. See Figure 25.

2 Connect the GC power cord to the outlet.

Figure 25 Cable for 6850 Series GC

6850 Series GC

G4513A Injector

Injector cable

Connect to top connector

(G4514-60610)

Power cord not shown

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

7820 MSD

Installation, Operati Part 2, Installation

Follow the instructions below to properly wire the injector and sample tray to the 7820 MSD.

1 Connect the injector to the GC with a G4514-60610 cable.

2 Connect the tray to the GC with a G4514-60610 cable.

3 Connect the GC power cord to the outlet.

Figure 26 Cables for 7820 MSD

Power cords not shown

Injector

Front

Inj1

Cables (G4514-60610)

7820 MSD

TraySampler1

Tray (Includes optional BCR)

7820A GC

on, and Maintenance 79

3 Installation

5975T LTM-GC/MSD

80

Follow the instructions below to properly wire the injector and sample tray to the 5975T LTM-GC/MSD.

1 Connect the injector to the GC with a G4514-60610 cable.

2 Connect the GC power cord to the outlet.

Figure 27 Cables for 5975T LTM-GC/MSD

Power cords not shown

Injector

Front

Inj1

Cable (G4514-60610)

5975T LTM-GC/MSD

Sampler1

Test the connections

When the cables are connected, turn on the GC power. After the start-up process ends:

The Ready light on the injector tower should be on.

If the Align Mode light on the injector tower is on, see Aligning the Injector.

If the Fault light on the sample tray is on, see Faults.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Parking the Sample Tray

Installation, Operati Part 2, Installation

If a sample tray is installed, follow the procedure below to park the sample tray. If you do not have a sample tray, skip this section.

1 Power on the GC.

2 Park the sample tray by pushing the [P] button on the sample tray front panel.

The gantry will move to the far-left position (away from the tray bracket), and gripper jaw will move to the far-back position (away from the tray front panel). This allows for clear access to the tray base.

To operate the sample tray, the gantry must be out of the Park position. Press [P] on the

NOTE tray front panel to move the gantry from the Park position to the Home position.

on, and Maintenance 81

3 Installation

Installing the Vial Racks

82

If a sample tray is installed, follow the procedure below to install the vial racks. If you do not have a sample tray, skip this section.

Install the vial rack labels

Before using the vial racks, you must install the vial rack labels:

1 Place the vial racks on a flat surface.

2 Align the vial rack label with the front of the vial rack so that the lip of the vial rack label fits over the front of the vial rack. The tabs on the underside of the vial rack label will align with the insert holes on the vial rack.

3 Press the vial rack label down into the vial rack until it snaps into place.

Vial rack label lip

Vial rack label

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Install the vial racks

Installation, Operati Part 2, Installation

1 After the vial rack labels are installed, lower the back end of the vial rack into the tray base.

on, and Maintenance 83

84

3 Installation

2 Align the tab found on the back side of the vial rack with the hole in the trays back wall.

3 Lower the front of the vial rack so that it fits into place and rests flat on the tray base. The LED light below each vial rack will light if the vial rack is

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

present. Be sure to install the vial racks so the vial rack label numbers are in sequential order from right to left.

4 Repeat the process for the two remaining vial racks.

Remove the vial rack labels

Skip this section if you do not wish to remove the vial rack labels. To remove the vial rack labels:

1 Hold the vial rack upside-down.

on, and Maintenance 85

86

3 Installation

2 Using your free hand, pinch the two tabs towards each other until the vial rack label snaps out of the vial rack.

Vial rack label tabs

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Updating the Firmware

Installation, Operati Part 2, Installation

The 7693A ALS requires the firmware revisions noted in Table 2. Before using the 7693A ALS system, check the firmware revisions as described below.

Use the Agilent Firmware Update tool to update GC and ALS firmware. The tool is available on the User Manuals & Tools DVD and from the Agilent web site. You can download the latest firmware revisions from the Agilent web site at www.agilent.com, or contact your local Agilent sales representative.

Failure to update the firmware will result in unrecognized components, vial delivery errors, or fewer available features.

View the current firmware version

To view the current firmware version for the GC or installed ALS components:

All GCs Power cycle the instrument. When it reboots, it displays the current firmware version.

Intuvo 9000 GC Touch [Settings] > [About].

7890 Series GC Press [Status] > [Clear], or press [Service Mode] > Diagnostics > Instrument status to display the current GC firmware version. To view the current ALS component firmware, press [Service Mode] > Diagnostics > ALS Status. Scroll through the menu to view the ALS controller, Front/Back Tower, Tray, and BCR firmware versions.

7820A GC, 7820 MSD Using the software keypad, press [Status] > [Clear], or press [Service Mode] > Diagnostics > Instrument status to display the current GC firmware version. To view the current ALS component firmware, press [Service Mode] > Diagnostics > ALS Status. Scroll through the menu to view the ALS controller, Front/Back Tower, Tray, and BCR firmware versions.

All 6890 GCs Press [Options], then select Diagnostics > Instrument Status. Scroll down to view the GC and ALS component firmware versions. For 6890A GCs the G4517A ALS Interface Controller, Front/Back Tower, Sample Tray, and BCR firmware versions are displayed. For 6890N GCs the sample tray and BCR firmware versions are displayed.

on, and Maintenance 87

88

3 Installation

5975T LTM-GC/MSD Press [Menu] to scroll to + Version or + LTM GC, and use the [Item] key to scroll to the desired component firmware information. Use an Agilent data system to view the injector firmware information.

Update the firmware

To update firmware, use the Agilent GC Firmware Update utility, available from the Agilent web site and from the Agilent User Manuals & Tools DVDs. Refer to the software help and user documentation for firmware update information.

Once you connect to an instrument in the softwares Firmware Update screen, no other tasks can be performed on the instrument until you disconnect.

6890A and 6890 Plus GCs

These GCs use programmable chips that must be physically replaced. Contact your local Agilent service representative.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Configuring the GC and Data System

Configure the GC

Installation, Operati Part 2, Installation

When hardware installation is complete, configure your GC for use with the 7693A ALS system. See ALS Configuration for details. Be sure to check:

Injector usage

Solvent bottle usage

Solvent volume usage

Configure the data system

Configuration

Agilent data systems contain information about the sampling equipment in use. This must be updated to remove the old information and replace it with information about the new equipment that you have installed. See your data system documentation for details.

Upgrading methods

Before using methods created for a previous sampler system, be sure to edit them as needed to match the new hardware.

on, and Maintenance 89

3 Installation

Calibrating the ALS System

90

If a sample tray is installed, follow the procedure below to calibrate the ALS system. If you do not have a sample tray, skip this section.

The ALS system calibration process aligns the sample tray with the injector turret position so vial transfers occur without incident. Calibration should be performed if a calibration does not exist, and also as a routine maintenance procedure.

Calibrating the ALS system is recommended if any ALS components are moved.

To calibrate your ALS system:

1 Place the calibration vial (G4514-40588) in tray position 1 (Figure 28).

Figure 28 Tray position 1

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Installation, Operati Part 2, Installation

2 Remove any vials from transfer turret positions L1, L2, and L3 for all installed injectors (Figure 29).

3 Start the ALS system calibration:

On a 7890A GC, use the GC front keypad and select [Options] Calibration > ALS > Start Calibration.

On a 6890 Series GC, use the GC front keypad and select [Options] Calibration > Sample tray > Start Calibration.

On an Intuvo 9000 GC:

Figure 29 Transfer turret positions L1, L2, L3 (top view)

L1

L2

L3

on, and Maintenance 91

92

3 Installation

The following calibration process will occur for all installed injectors:

a The tray assesses the turret alignment by placing the calibration vial into turret position L1 and returning to sample tray position 1.

b The tray tests the vial height and turret position by using the alignment tab between positions L1 and L2 on the transfer turret.

c The tray verifies the turret alignment by placing the calibration vial into turret position L1 and returning it to sample tray position 1.

4 When the calibration process is complete, the green Ready status light will come on and the gantry will stop in the Home position (Figure 66 on page 222).

Remember to replace any vials that were moved for the calibration process.

Installation, Operation, and Maintenance Part 2, Installation

Installation 3

Making a Trial Run

Installation, Operati Part 2, Installation

Once installation, configuration, updating, and calibration is complete, make a quick injection using the sampler to verify that it works properly.

1 Install an empty syringe in the injector.

2 If using the G4514A Sample Tray with the G4513A Injector, make sure the transfer turret is installed. See Changing the Turret for details.

If using only the G4513A Injector, replace the transfer turret with the standalone turret, if desired. See Changing the Turret for details.

3 Place empty bottles in all the Solvent A and Waste A turret positions. Place an empty capped sample vial in the tray 1 position (or the turret sample 1 position, if not using the sample tray).

4 Make the sampler settings shown in Table 3. These are designed for a 7890 Series GC. If you are using a different GC, use these settings as a guide.

For 7890A and 6890 GCs Use the front keypad

For 6850 GCs Use your Agilent data system

Table 3 Trial run parameters

Parameter Setting

Injection volume 1.00

Viscosity delay 0

Inject Dispense Speed 6000

Airgap Volume 0.20

Sample Pumps 6

Sample Washes 0

Solvent A post washes 1

Solvent A pre washes 1

Solvent B post washes 0

Solvent B pre washes 0

Sample Draw Speed 300

Pre dwell time 0

Post dwell 0

Sample offset 0

Injection Mode Normal (1-layer injection)

on, and Maintenance 93

94

3 Installation

5 Set the GC oven program to 30 C (or current room temperature) with a 0 C/min ramp, a hold time of 0.1 minutes, an equilibrium time of 0.3 minutes, and an initial time of 0.3 minutes.

6 Store, load, then run the sequence.

If there are no faults, the injector will make one injection from the first vial position.

If problems occur, see Faults, Error Messages, Correcting Syringe Problems, or Correcting Sample Vial Delivery Problems.

Tower LED On

Table 3 Trial run parameters (continued)

Parameter Setting

Installation, Operation, and Maintenance Part 2, Installation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

4 Accessories

Installing the G4526A/G4517A ALS Controller (6890A GCs) 96

Installing the G4526A/G4516A ALS Interface Controller (6890 Plus GCs) 101

Installing the G4515A Bar Code Reader/Mixer/Heater 105

Installing the G4522A Heating and Cooling Plate 116

The procedure for installing the 7693A ALS accessories depend on the system components purchased and the type of GC involved. Follow the steps in this chapter that are relevant to your GC and ALS system setup.

95Agilent Technologies

4 Accessories

Installing the G4526A/G4517A ALS Controller (6890A GCs)

96

This procedure applies to the 6890A GC only. If you do not have a 6890A GC, skip this section.

Controller site requirements

This procedure explains how to install the G4517A ALS Controller on a 6890A GC.

Temperature and humidity ranges

The ALS system is designed for indoor use only in ordinary atmospheres.

After exposing the ALS controller to extremes of temperature or humidity, allow 15 minutes for it to return to the recommended ranges.

Ventilation requirements

The controller is cooled by air flow which enters the front of the instrument and exits through the rear. Do not4obstruct air flow in the front or rear of the instrument.

Temperature range 5 C to 55 C

Relative humidity range Maximum relative humidity of 80% for temperatures up to 31 C decreasing linearly to 50% relative humidity at 40 C

Altitude range Up to 4300 m

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

Benchtop space requirements

Figure 30 shows the space requirements around the controller. The area in the back of the controller must be kept clear for easy operation of the power switch and the front of the controller requires access to the front panel buttons.

Dimensions

25 cm (9.84 in) wide 31 cm (12.2 in) deep 11 cm (4.33 in) high 5 kg (11 lbs) weight

Figure 30 Benchtop space requirements

15 cm minimum

31 cm

25 cm

Front

G4517AA Controller

on, and Maintenance 97

98

4 Accessories

Orientation

The G4517A ALS Controller is designed to be installed in a horizontal orientation as shown in Figure 31. If mounted in the vertical orientation, the probability of tipping increases with the possibility of injury to personnel.

Electrical requirements

Figure 31 ALS controller orientation

Horizontal recommended Vertical

ALS Controller

ALS Controller

AL S

Co nt

ro lle

r

A proper earth ground is required.

CAUTION

Grounding To protect users, the cabinet is grounded through the three-conductor power line cord in accordance with International Electrotechnical Commission (IEC) requirements.

The three-conductor power line cord, when plugged into a properly grounded receptacle, grounds the instrument and minimizes shock hazard. A properly grounded receptacle is one that is connected to a suitable earth ground. Proper receptacle grounding should be verified.

Any interruption of the grounding conductor or disconnection of the power cord

WARNING could cause a shock that could result in personal injury.

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

Line voltage The G4517A ALS Controller operates from one of the AC voltage supplies listed in Table 4, depending on the standard voltage of the country from which it was ordered. It is designed to work at a specific voltage; make sure your instruments voltage option is appropriated for your lab. The voltage requirement is printed near the power cord attachment. See Verify the power configuration on page 100 for setting the proper voltage for this controller.

Table 4 Voltage requirement by country

Country Voltage Part number

Americas, 10 amp 120 V 8120-1378

Argentina 220 V 8120-6869

Australia, 10 amp 240 V 8120-1369

Chile, 10 amp 220 V 8120-6978

China, 10 amp 220 V 8121-0723

DK/Greenland, 10 amp 220 V 8120-3997

Europe, 10 amp 230 V 8120-1689

GB/HK/SG/MY, 10 amp 240 V 8120-8705

India/S Africa, 10 amp 240 V 8120-4211

Israel, 10 amp 220 V 8120-5182

Japan, 10 amp 200 V 8120-4753

Korea, 10 amp 220 V 8121-1226

Switzerland, 10 amp 230 V 8120-2104

on, and Maintenance 99

4 Accessories

Verify the power configuration

100

The G4517A ALS Controller can be configured for either 110120 V power or for 220240 V power.

CAUTION Failure to configure the correct voltage setting will blow the fuses.

To determine the current power configuration, look at the back panel of the controller. The fuse holder module below the power cord receptacle is labeled with 220240 V on one edge and with 110120 V on the other (Figure 32).

To change the power configuration, remove the fuse holder module using a small, flat-bladed screwdriver in the notch at the top of the fuse holder. Turn it over and reinstall. See Figure 32.

Figure 32 G4517A power configurations

USE ONLY WITH 250V FUSES/EMPLOYER UNIQUEMENT AVEC

DES FUSIBLES DE 250V 110-120 V

220-240 V

USE ONLY WITH 250V FUSES/EMPLOYER UNIQUEMENT AVEC

DES FUSIBLES DE 250V 110-120 V

220-240 V

Indicates current voltage configuration

Configured for 110-120 V Configured for 220240 V

Notch

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installing the G4526A/G4516A ALS Interface Controller (6890 Plus GCs)

Installation, Operati Part 2, Installation

This procedure explains how to install the G4516A ALS Interface controller board on the 6890 Plus GC. If you do not have a 6890 Plus GC, skip this section.

The G4516A ALS Interface controller board must be present in a 6890 Plus GC to operate the 7693A ALS. The GC must have a serial number greater than 20,000.

WARNING Before proceeding, turn off the main power switch and unplug the power cord.

CAUTION Make sure you are properly grounded with an ESD strap before continuing.

1 Remove the GC back panels and the right side cover.

CAUTION Attempting to slide the entire board under the locking tab will damage the boards components.

on, and Maintenance 101

102

4 Accessories

2 Hold the board upright and slightly angled (Figure 33).

3 Place the board onto the mounting bracket.

4 Slide it into the bracket until the cutouts in the board are aligned with the locking tabs in the bracket.

5 Lay the board against the chassis then slide it in until it stops. The locking tabs should hold the board in place.

6 Secure the board to the chassis using two screws. The board should not be stressed or bowed against the locking tab (Figure 33).

7 Locate the 2-wire cable leading from the transformer and connect it to the ALS Interface board at J5. (Figure 33 and Figure 34).

Figure 33 Installing the ALS interface board

Step 2. Hold board at an angle

Step 3. Place board in bracket

Step 4. Align tabs and cutouts

Step 5. Insert board to stopsStep 6.

Secure board with screws

Step 7. Connect transformer cable

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

8 If an MIO card (LAN card) is installed, loosen its two mounting screws and slide the card out of the GC (Figure 35).

Figure 34 ALS interface connectors

Figure 35 Removing the LAN card

J5, cable from transformer (step 7) connects here

P5, controller cable (step 9) connects here

on, and Maintenance 103

104

4 Accessories

9 Connect the Controller PCB Cable, part no. G2612-60510, to the main board at J8 and to the ALS Interface board at P5. Route the cable through the cutout in the main board (Figure 34 and Figure 36).

10 If an MIO card was removed from the GC, re-install it.

11 Using a nut driver, remove the cover plate over the three holes labeled Injector 1 (default front), Injector 2 (default back), and Tray from the rear panel. Save the plate and nuts so you can reinstall them to prevent access to the high voltage area of the GC if you ever remove the ALS interface board in the future.

12 Re-install the GC covers.

Figure 36 GC main board

J8, controller cable connects here

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installing the G4515A Bar Code Reader/Mixer/Heater

Installation, Operati Part 2, Installation

If you have purchased a separate G4515A Bar Code Reader/Mixer/Heater accessory, install it now. Otherwise skip this section.

Remove the sample tray from the GC

1 Set the GC inlets, detectors, and oven to room temperature.

2 Park the sample tray by pressing the [P] button on the front tray panel.

3 After the GC inlets, detectors, and oven have cooled, turn off the GC and unplug the power cord.

4 Unplug the sample tray cable.

5 Unplug all injector cables.

6 Remove all vials from the injector turrets.

7 Remove all injectors from the GC inlet area. If desired, mount the injectors on any available parking posts.

8 Remove all vial racks from the sample tray.

9 Completely remove the three T-30 Torx screws securing the sample tray to the mounting bracket.

10 Carefully remove the sample tray from the GC mounting bracket.

on, and Maintenance 105

4 Accessories

Install the G4515A Bar Code Reader/Mixer/Heater

106

1 With the gantry in the far-left position, loosen the two T-20 screws securing the unknown vial station cover, and remove the plastic cover.

T-20 screws

Unknown vial station cover

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

2 Slide the gantry towards the tray bracket as far as it will go.

on, and Maintenance 107

108

4 Accessories

3 Loosen the T-20 Torx screw and remove tray cover.

T-20 screw

Installation, Operation

, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

4 Set the tray cover on a flat surface and remove the T-10 Torx screw that fastens the blanking plate to the tray cover. The blanking plate will fall to the surface below the tray cover when the screw is completely removed.

T-10 screw

on, and Maintenance

109

110

4 Accessories

5 Move the G4514-60601 cable to the side.

6 Place the Bar Code Reader in the tray and secure with three screws.

G4514-60601 cable

3 screws

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

7 Connect the BCR ribbon cable and power cable.

From tray

From BCR to tray

to BCR

on, and Maintenance 111

112

4 Accessories

8 Reinstall the tray cover. Make sure the tray cover edge does not pass the outer housing edge. Push the tray cover towards the front of the tray as far as possible before installing screws.

Outer housing edge

Tray cover edge

Installation, Oper

ation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

9 Install the T-20 Torx screw.

T-20 screw

on, and Maintenance

113

114

4 Accessories

10 Install the self-tapping screw (0624-0681) supplied with the BCR accessory kit using a T-10 Torx driver.

T-10 screw

Installation, Operation

, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

11 Slide the gantry to the opposite end of the tray, and replace the unknown vial station cover.

12 Tighten the two T-20 Torx screws to secure the unknown vial station cover to the sample tray.

13 If you wish to install an optional 1/8-in ID heater vent line, remove the bottom access panel on the underside of the sample tray using a T-20 Torx driver. Otherwise, skip to the next section.

14 Remove the Heater Vent Base (G4515-20532) using a straight blade screwdriver.

15 Attach the 1/8-in ID vent line to the BCR, and route the vent tubing out through the bottom access panel. Three wire saddles (1400-3408) and three M3 x 4 screws (0515-0663) are provided to help route the vent tubing.

T-20 screws

Unknown vial station cover

on, and Maintenance 115

4 Accessories

Installing the G4522A Heating and Cooling Plate

116

This section describes how to install the G4522A Heating And Cooling Plate on the G4514A Sample Tray.

This section does not explain how to set up a water bath and pump.

You can control the temperature of the sample vials in the tray by pumping temperature-controlled liquid through the heating and cooling plate installed underneath the vial racks.

Tools needed

CAUTION Ensure that the drainage tube is attached to the base of the sample tray. If possible, remove any equipment from underneath the tray or take steps to protect any equipment underneath the tray from potential leaks. When controlling for low sample temperature with high ambient temperature or high humidity, water condensation from the heating and cooling plate and sample tray could damage equipment under the sample tray.

Diagonal cutters

T-10 Torx driver

T-20 Torx driver

T-30 Torx driver

Remove the sample tray from the GC

1 Set the GC inlets, detectors, and oven to room temperature.

2 Park the sample tray by pressing the [P] button on the front tray panel.

3 After the GC inlets, detectors, and oven have cooled, turn off the GC and unplug the power cord.

4 Unplug the sample tray cable.

5 Unplug all injector cables.

6 Remove all vials from the injector turrets.

7 Remove all injectors from the GC inlet area. If desired, mount the injectors on any available parking posts.

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

8 Remove all vial racks from the sample tray.

9 Completely remove the three T-30 Torx screws securing the sample tray to the mounting bracket.

10 Carefully remove the sample tray from the GC mounting bracket.

Install the heating and cooling plate

1 Make sure the gantry is in the park position (far-left, away from the tray bracket).

2 Completely loosen the two T-20 screws securing the unknown vial station cover.

3 Remove the unknown vial station cover.

4 Completely loosen the two T-20 screws securing the unknown vial station.

T-20 screws

Unknown vial station cover

on, and Maintenance 117

118

4 Accessories

5 Remove the unknown vial station.

6 Slide the gantry towards the home position (far-right, towards the tray bracket).

Calibration post

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

7 Loosen the T-20 Torx screw securing the tray cover.

8 Remove the tray cover.

9 Slide the gantry towards the park position (far-left, away from the tray bracket).

T-20 screw

Tray cover

on, and Maintenance

119

120

4 Accessories

10 Loosen and remove the four screws securing the vial rack support plate.

T-20 screws

Vial rack support plate

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

11 Remove the support plate by lifting the front of the plate and pulling outward towards the front of the sample tray. The support plate will slide out.

on, and Maintenance 121

122

4 Accessories

12 Place the sample tray on its back and remove the bottom access panel using a T-20 Torx driver.

13 Using a diagonal cutter, clip the metal tabs and remove the accessory plate from the underside of the tray chassis.

Bottom access panel

Cooling accessory plate

T-20 screw

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

14 Place the sample tray on its base and position the heating and cooling plate partially in place, leaving room to run the heating and cooling plate cable out of the tray chassis through the bottom access opening.

Heating and cooling plate

Cable through bottom access opening

on, and Maintenance 123

124

4 Accessories

15 Once the cable is accessible through the bottom access opening, fit the heating and cooling plate in place so the top is level with the tray chassis.

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

16 Secure the heating and cooling plate to the tray chassis using four screws (two in front, two in back).

T-20 screws

T-20 screws

Back of tray

on, and Maintenance 125

126

4 Accessories

17 Place the sample tray on its back and connect the heating and cooling plate cable to the tray logic board (accessible through the bottom access opening).

18 Reinstall the bottom access panel.

Connect cable to tray board

Reassemble the sample tray

1 If necessary, slide the gantry to the home position (far-right, towards the tray mount).

2 Replace the sample tray cover and secure it with a T-20 Torx screw.

3 Slide the gantry to the park position (far-left, away from the tray mount).

4 Replace the unknown vial station and secure it with two T-20 Torx screws.

5 Replace the unknown vial station cover and secure it with two T-20 Torx screws.

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

6 Install the heating and cooling plate vial racks. See Installing the Vial Racks for details.

7 Install the sample tray on the GC. See Installing the Sample Tray for details.

Install the drainage tubing

1 Connect the plastic tubing to the heating and cooling plates drainage nipple on the back of the sample tray. The tubing must allow for the heating and cooling plate to drain condensate easily with no back pressure. Make sure that:

The tubing slopes downward towards the drainage container.

The tubing is kept straight without kinks that may block the flow.

The open end of the tubing is not submersed in the drainage container (Figure 37).

on, and Maintenance 127

128

4 Accessories

The tubing does not become clogged or dirty. Replace tubing if necessary.

2 Secure the tubing to the back of the heating and cooling plate using a 1/4-in Swagelok connection.

3 Check for any leaks before powering on the GC.

Figure 37 Drainage tube correctly hung (left) and incorrectly submersed (right)

Complete the installation

1 Install the injectors. See Installing the G4513A Injector for details.

2 Connect the GC power cable, and power on the GC.

3 Calibrate the ALS system. See Calibrating the ALS System for details.

Coolant

Use only distilled water, ethylene glycol, or propylene glycol as coolant.

Water bath and pump specifications

To limit burn hazards, the heating liquids should not exceed 60 C.

CAUTION

Installation, Operation, and Maintenance Part 2, Installation

Accessories 4

Installation, Operati Part 2, Installation

The water bath and pump system used to control the sample vial temperatures must meet the following specifications:

The components must meet national standards for safety requirements, be suitable for unattended operation, be suitable for continuous operation, and be controllable for high-temperature protection.

The recommended coolant temperature range is 5 to 60 C

If you use a built-in pump, it must be suitable for external circulation of liquid and for connection of 1/4-in od (6.35 mm) tubing or larger.

If you use a pressure pump, it must maintain a pressure from 1.5 to 2.5 psi.

If you use a suction pump, the pump vacuum cannot exceed 4 psi.

Typical recirculator cooling power capacity varies from 1000 to 2000 watts.

NOTE The temperature sensor detects the average coolant temperature in the heating and cooling plate.

on, and Maintenance 129

130

4 Accessories

Figure 38 Pressure vs. Water Flow

Installation, Operation, and Maintenance Part 2, Installation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

Part 3:

Operation

Introduction to Operation 133

About your 7693A Automatic Liquid Sampler 134

Fast Injection 140

Sample Carryover 142

Methods and Sequences 144

The Sampler Cycle 145

ALS Configuration 147

Configuring the Injector 148

Configuring the Sample Tray 153

ALS Parameters 161

Setting the Injector Parameters 162

Setting the Sample Tray Parameters 169

Syringes and Needles 173

Selecting a Syringe 174

Inspecting a Syringe 177

Installing a Syringe 178

Removing a Syringe 182

Replacing a Syringe Needle 183

Vials and Bottles 185

Preparing a Sample Vial 186

Preparing Solvent and Waste Bottles 192

Placing Vials and Bottles in the Turret 194

Using Two Injectors (7890A and 6890 Series GCs Only) 197

How Many Sample Vials Can I Run? 198

Sandwich Injections 204

Running Samples 211

131Agilent Technologies

132

Running a Sample 212

Using the ALS Controller 213

Interrupting a Run or Sequence 214

Running a Priority Sample 215

Installation, Operation, and Maintenance

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

5 Introduction to Operation

About your 7693A Automatic Liquid Sampler 134

Components 134

Features 136

Capabilities 139

Fast Injection 140

Sample Carryover 142

Solvent wash 142

Sample wash 142

Sample pump 142

Number and type of washes 142

Methods and Sequences 144

The Sampler Cycle 145

This chapter describes the components that make up the Agilent 7693A ALS, some of the important features of the system, and the sampler capabilities.

133Agilent Technologies

5 Introduction to Operation

About your 7693A Automatic Liquid Sampler

Components

134

The 7693A ALS system (Figure 39) can include:

G4513A Injector module (one or two)

G4514A Sample Tray (7890 Series GC, 6890 Series GC, 7820 MSD)

G4514A Sample Tray (Intuvo 9000 GC)

G4515A Bar Code Reader/Mixer/Heater (BCR)

G4517A ALS Controller (6890A GC)

G4516A ALS Interface Board (6890 Plus GC)

G4520A Sample Tray with pre-installed BCR/Mixer/Heater

G4521A Enhanced Sample Handling Syringe Carriage

G4522A Heating and Cooling Plate

G4525A Set of 3 colored vial rack labels

G4526A 7693A Upgrade Kit for 6890 Series GCs

Installation, Operation, and Maintenance Part 3, Operation

Introduction to Operation 5

Installation, Operati Part 3, Operation

Figure 39 The 7693A ALS with 7890 Series GC and 5975 MSD

on, and Maintenance 135

5 Introduction to Operation

Features

136

The key features of the ALS system include:

Two interchangeable sample turrets provided with the injector:

The standalone turret allows you to analyze up to 16 samples. It holds two solvent positions and one waste bottle position. Two sample positions can be alternately configured for sample preparation. This turret is not compatible with the sample tray.

The transfer turret is designed to work with the sample tray to analyze up to 150 samples. The transfer turret has three sample vial transfer locations; two of which are configurable for use in sample preparation. There are six solvent A positions, four solvent B positions, and five waste positions. This turret can be used with or without the sample tray.

Accessible solvent and waste vial capacity of 20 mL

Ability to run sandwich injections with up to three sample layers separated with air gaps

A standard syringe carriage for syringes up to 100 L

An optional enhanced sample handling syringe carriage with a slow, high-powered motor for syringes over 100 L

Cool on-column injections into 250-m, 320-m, and 530-m columns

An optional BCR/Mixer/Heater module

An optional heating and cooling plate for the sample tray

The ability to inject different sample amounts from different sample vials

The ability to interrupt a sequence to run priority samples, then resume the sequence

Installation, Operation, and Maintenance Part 3, Operation

Introduction to Operation 5

Installation, Operati Part 3, Operation

Fast, slow, and variable plunger speeds under Agilent data system control (Table 5 and Table 6)

The default values for variable plunger speeds are based on a 10 L syringe. You should reduce the injection speed to accommodate larger syringe volumes. If you are performing a large volume injection and see a plunger error, try slowing the inject rate.

Table 5 Fast/slow plunger speed as a function of syringe volume

Plunger Speed (L/min)

Syringe Volume (L)

0.5 1 2 5 10 25 50 100 250 500

Draw (L/min)

Fast 15 30 60 150 300 750 1500 3000 300 600

Slow 15 30 60 150 300 750 1500 3000 300 600

Dispense (L/min)

Fast 300 600 1200 3000 6000 15000 30000 60000 1500 3000

Slow 300 600 1200 3000 6000 15000 30000 60000 1500 3000

Inject (L/min)

Fast 300 600 1200 3000 6000 15000 30000 60000

Slow 15 30 60 150 300 750 1500 3000 75 150

on, and Maintenance 137

138

5 Introduction to Operation

Table 6 Variable plunger speed as a function of syringe volume

Variable Plunger Speed (L/min)

Syringe Volume (L)

(x) = Default value 0.5 1 2 5 10 25 50 100 250 500

Sample draw 1 - 30 (15)

1 - 60 (30)

1 - 120 (60)

1 - 300 (150)

1 - 600 (300)

1 - 1500 (750)

3 - 3000

(1500)

6 - 6000

(3000)

15 - 15000 (7500)

30 - 30000

(15000)

Sample dispense 1 - 300 (300)

1 - 600 (600)

1 - 1200 (1200)

1 - 3000 (3000)

1 - 6000 (6000)

1 - 15000

(15000)

3 - 30000

(30000)

6 - 60000

(60000)

15 - 150000

(150000)

30 - 300000

(300000)

Injection dispense

1 - 300 (300)

1 - 600 (600)

1 - 1200 (1200)

1 - 3000 (3000)

1 - 6000 (6000)

1 - 15000

(15000)

3 - 30000

(30000)

6 - 60000

(60000)

15 - 150000

(150000)

30 - 300000

(300000)

Solvent draw 1 - 30 (15)

1 - 60 (30)

1 - 120 (60)

1 - 300 (150)

1 - 600 (300)

1 - 1500 (750)

3 - 3000

(1500)

6 - 6000

(3000)

15 - 15000 (7500)

30 - 30000

(15000)

Solvent dispense 1 - 300 (300)

1 - 600 (600)

1 - 1200 (1200)

1 - 3000 (3000)

1 - 6000 (6000)

1 - 15000

(15000)

3 - 30000

(30000)

6 - 60000

(60000)

15 - 150000

(150000)

30 - 300000

(300000)

Installation, Operation, and Maintenance Part 3, Operation

Introduction to Operation 5

Capabilities

Installation, Operati Part 3, Operation

Table 7 summarizes the capabilities of the 7693A ALS.

Table 7 ALS capabilities

Parameter Range

Syringe size 1 to 500 L

Wash mode A, B A - A2, B - B2 A - A6, B - B4

Solvent saving 10%, 20%, 30%, 40%, 80% of syringe size (L)

Injection volume 1 - 50% of syringe size (L)

Sample pumps 0 - 15

Viscosity delay 0 - 7 seconds

Air gap 0 - 10% of syringe size (L)

Pre-injection sample washes 0 - 15

Post-injection solvent A washes 0 - 15

Post-injection solvent B washes 0 - 15

Plunger speed Refer to Table 6 on page 138

Pre-injection solvent A washes 0 - 15

Pre-injection solvent B washes 0 - 15

Pre-injection dwell 0 - 1 minutes in .01 minute

Post-injection dwell 0 - 1 minutes in .01 minute

Sampling offset On, Off

Variable sampling depth position 2 mm below, 30 mm above

Multiple injection mode, number of injections 1 - 99

Multiple injection mode, injection delay time 0 - 100 seconds

on, and Maintenance 139

5 Introduction to Operation

Fast Injection

140

This is a method of introducing a sample to a heated inlet without the negative effect of needle fractionation.

If you are using the ALS for the first time, you may see some changes in the resulting chromatograms. Most of the changes are due to reducing the amount of vaporization from the needle during injection.

The peak areas of your chromatograms may be smaller. Automatic fast injection delivers the desired setpoint volume of sample. Without fast injection, residual amounts of sample boil out of the needle and enter the inlet. This extra amount could measure up to 1 L.

The peak areas of your chromatograms may show less differentiation between the low boiling and high boiling components.

Without fast injection, the sample introduced is richer in low boiling component than in high boiling components because of fractional distillation in the needle. Not only does residual sample in the needle enter the inlet, but the low boiling components boil off first. This is needle fractionation or discrimination.

Installation, Operation, and Maintenance Part 3, Operation

Introduction to Operation 5

Installation, Operati Part 3, Operation

Figure 40 compares manual injection with automatic fast injection from the ALS for a 1-L sample of C10 to C40 paraffins in hexane.

For more information on the performance of the ALS, order the following technical papers from your Agilent representative:

Publication No. 43-5953-1843:Snyder, W.Dale. Fast Injection with the 7673A Automatic Injector: Chemical Performance, Technical Paper 108, June 1985.

Publication No. 43-5953-1878:Snyder, W.Dale. Performance Advantage of the 7673A Automatic Injector Over Manual Injection, Technical Paper 109, August 1985.

Publication No. 43-5953-1879:Kolloff, R.H.C.Toney, and J.Butler. Automated On-Column Injection with Agilent 7673A Automatic Injector and 19245A On-Column Capillary Inlet: Accuracy and Precision, Technical Paper 110, August 1985.

Figure 40 Automated vs. manual injection

Automated injection

Manual injection

on, and Maintenance 141

5 Introduction to Operation

Sample Carryover

142

Carryover is the presence of peaks from an earlier injection in the present analysis.

The injector uses solvent washes, sample washes, and sample pumps to control carryover. Each of these actions reduces the amount of sample left in the syringe. The effectiveness of each depends on your application.

Solvent wash

The injector draws solvent into the syringe from either the solvent A positions or solvent B positions, then discards the syringe contents into one or more waste bottles. Solvent washes can occur before taking a sample (pre-injection solvent wash) or immediately after the injection (post-injection solvent wash). The volume of the wash can be adjusted.

Sample wash

During a sample wash, the injector draws the next sample into the syringe and discards the contents into one or more waste bottles. Sample washes occur before the injection. When sample is limited, you can use a solvent prewash to wet the syringe before drawing sample. The volume of the wash can be adjusted.

Sample pump

During a sample pump, the injector draws sample into the syringe and returns it to the sample vial. Pumps occur after sample washes and immediately before the injection. Pumps serve to eliminate bubbles. If the needle contains solvent from a previous wash, the pump may add a small amount of solvent that mixes with the sample and can dilute a small volume.

Number and type of washes

Under ideal conditions, four default volume (80%) washes reduce the carryover to one part in 10,000. The number and type of washes you need depends on:

The amount of carryover that you can accept

The viscosity and solubility of the analyte(s)

The viscosity and volatility of the solvent(s)

Installation, Operation, and Maintenance Part 3, Operation

Introduction to Operation 5

Installation, Operati Part 3, Operation

The degree of wear in the syringe barrel

The wash volume

Chromatograms A and B (Figure 41) show the effect of carryover when 1 L from a vial of methanol is injected after 1 L from a vial of a solute dissolved in methanol. The peaks in chromatogram B are from the solute left in the syringe from the first injection.

Chromatogram C shows the result of washing the syringe with four 80% syringe volume solvent washes. The carryover peaks disappear.

Figure 41 Sample carryover

Chromatogram A: 20 mg/mL of solute in methanol

Chromatogram B: Methanol blank without washes

Chromatogram C: Methanol blank after four washes

on, and Maintenance 143

5 Introduction to Operation

Methods and Sequences

144

The sampler is usually controlled by a sequence, which is a list of samples to be analyzed. It contains:

Where to find the sample

What method to use to analyze the sample

How to measure and inject the sample

How to generate a report of analysis

A method is a collection of setpoints (temperatures, times, etc.) that controls the operation of a gas chromatograph.

The combination of a sequence and the methods it specifies provides complete control over the analysis of your samples. However, the specific details differ depending on the hardware and software used, so you are referred to other documents for that information.

This discussion is limited to sampler-specific details. For setting up methods and sequences see your Agilent data system help or GC documentation.

Installation, Operation, and Maintenance Part 3, Operation

Introduction to Operation 5

The Sampler Cycle

Installation, Operati Part 3, Operation

All versions of the ALS perform the same basic operations (Table 8).

Table 8 Sampler cycle

Step Action Notes

1 Transport the sample vial. If necessary, move the sample vial from the tray to the turret.

2 Wash the syringe with solvent. a Rotate a solvent bottle under the syringe.

b Lower the syringe needle into the solvent.

c Draw in solvent. d Raise the syringe needle out of the

solvent bottle. e Rotate a waste bottle under the

syringe. f Lower the syringe. Depress the

plunger to discard solvent. g Raise the syringe needle out of the

waste vial.

The syringe can be washed several times and with more than one solvent. This is controlled by the solvent pre-wash parameters.

3 Wash the syringe with sample. a Rotate the sample vial under the syringe.

b Lower the syringe needle so that the needle pierces the vial septum and enters the sample.

c Draw in sample. d Raise the syringe and needle out of

the solvent bottle. e Rotate a waste bottle under the

syringe. f Lower the syringe needle. Depress

the plunger to discard sample. g Raise the syringe needle out of the

waste vial.

The syringe can be rinsed with sample several times.

on, and Maintenance 145

5 Introduction to Operation

4 Load the syringe with sample. a Rotate the sample vial under the syringe.

b Lower the syringe needle so that it pierces the vial septum.

c Draw in sample. d With the needle still in the sample,

depress the syringe plunger quickly. e After the final pump, draw in

sample. f Raise the syringe needle out of the

sample vial.

Actions c and d can be repeated several times. The purpose is to expel air bubbles from the syringe.

5 Inject the sample. a Rotate the turret to expose the GC inlet.

b Lower the syringe needle so that it pierces the inlet septum.

c Depress the syringe plunger to make the injection.

d Raise the syringe needle out of the GC inlet.

Start signals are sent to the GC and the data processor at the moment of injection.

6 Wash the syringe with solvent. Same as step 2, but according to the post-injection parameters.

7 Transport the sample vial. Return the sample vial to its initial turret or tray location.

8 Perform repeat injections. If so programmed, wait for the GC to become Ready and repeat the cycle from step 1.

Table 8 Sampler cycle (continued)

Step Action Notes

146

Installation, Operation, and Maintenance Part 3, Operation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

6 ALS Configuration

Configuring the Injector 148

7890 Series GC, 7820A GC, and 7820 MSD 148

6890 Series GC 149

6850 Series GC 151

5975T LTM-GC/MSD 152

Configuring the Sample Tray 153

7890 Series GC and 7820 MSD 153

6890 Series GC 155

Configure the ALS on an Intuvo 9000 GC 158

This chapter describes how to configure the ALS using different controlling devices.

The descriptions in this chapter refer to the features available with the GC firmware and does not necessarily describe the capabilities available with Agilent data systems. Refer to Agilent data system online help.

147Agilent Technologies

6 ALS Configuration

Configuring the Injector

7890 Series GC, 7820A GC, and 7820 MSD

148

For a 7890 Series GC, press [Config] [Front Injector] or [Config] [Back Injector] on the GC keypad to display the front or back injector configuration parameters. For a 7820A GC or 7820 MSD, press [Config] [Injector] on the GC software keypad to display the front or back injector configuration parameters.

Wash Mode Bottles This is only displayed when the injector has a transfer turret installed. On a 7890 Series GC the turret solvent bottle positions are selected using the [Mode/Type] key:

6-A and 4-B WashesUses all 6 solvent A bottles if injector uses solvent A washes. Uses all 4 solvent B bottles if injector uses solvent B washes. The injector alternates between all bottles.

2-A and 2-B WashesUses solvent bottles A and A2 if injector uses solvent A washes and solvent bottles B and B2 if injector uses solvent B washes. The injector alternates between all bottles.

1-A and 1-B WashesUses solvent bottle A if injector uses solvent A washes and solvent bottle B if injector uses solvent B washes.

The injector always uses both WA waste bottles if a solvent A wash is used and both WB waste bottles if a solvent B wash is used.

Syringe size Enter a syringe size between 0.5 and 500 L.

CONFIGURE FRONT INJECTOR

Wash Mode Bottles 1 A, 1 B Syringe Size 10.0

The GC assumes the syringe volume represents full plunger stroke. If the maximum volume

NOTE marked on the syringe barrel is halfway up the barrel, you will need to enter double that volume (the label in this case is half of the full plunger stroke).

Installation, Operation, and Maintenance Part 3, Operation

ALS Configuration 6

6890 Series GC

Installation, Operati Part 3, Operation

Press [Config] [Front Injector] or [Config] [Back Injector] to display the parameters.

Scroll to the desired parameter detailed below.

Inject modeSelect the injection mode type that is installed on your injector. On a 6890N GC the injection mode is selected using the [Mode/Type] key:

Normal-Injects a single sample.

Sample + L2-Injects a mixture of two samples separated by an air gap.

Sample + L2 + L3-Injects a mixture of three samples separated by air gaps.

Large Volume-Injects sample using a 250 or 500 L syringe.

Wash mode This is only displayed when the injector has a transfer turret installed. On a 6890N GC the turret solvent bottle positions are selected using the [Mode/Type] key:

Wash using A, BUses solvent bottle A if injector uses solvent A washes and solvent bottle B if injector uses solvent B washes.

Use A-A2, B-B2Uses solvent bottles A and A2 if injector uses solvent A washes and solvent bottles B and B2 if injector uses solvent B washes. The injector alternates between both bottles.

Use All A, BUses all 6 solvent A bottles if injector uses solvent A washes. Uses all 4 solvent B bottles if injector uses solvent B washes. The injector alternates between all bottles.

For a 6890A or 6890 Plus GC, the wash mode is controlled by scrolling through the options using the [On] key, or entering a value of:

CONFIG F INJECTOR

Inject mode Normal Wash mode A, B Solvent saving Off

Syringe size 10.0 Injector Light On

on, a

nd Maintenance 149

150

6 ALS Configuration

1 for using solvent bottle A if injector uses solvent A washes and solvent bottle B if injector uses solvent B washes.

2 for using solvent bottles A and A2 if injector uses solvent A washes and solvent bottles B and B2 if injector uses solvent B washes. The injector alternates between all bottles.

3 for using all solvent A bottles if injector uses solvent A washes and all solvent B bottles if injector uses solvent B washes. The injector alternates between all bottles.

The injector always uses both WA waste bottles if a solvent A wash is used and both WB waste bottles if a solvent B wash is used.

Solvent SavingUse this option to vary the syringe wash amount from about 10% to 80% of syringe volume. This setting reduces the amount of solvent and sample consumed during each injection cycle. See "Sample Carryover" on page 142 for more information.

On a 6890N, the wash amount is selected using the [Mode/Type] key. This brings up a selection of actual wash volumes based on the volume of the syringe used. Select Off to disable solvent saving option.

For a 6890A or 6890 Plus, these settings are controlled by scrolling through the options using the [On] key, or entering a setpoint value of:

0 to disable the solvent saving feature resulting in a wash volume equal to 80% of syringe volume.

1 for 10% of syringe volume wash

2 for 20% of syringe volume wash

3 for 30% of syringe volume wash

4 for 40% of syringe volume wash

Syringe sizeEnter a syringe size between 0.5 and 500 L.

NOTE The GC assumes the syringe volume represents full plunger stroke. If the maximum volume marked on the syringe barrel is halfway up the barrel, you will need to enter double that volume (the label in this case is half of the full plunger stroke).

Injector LightControl the LED light (On, Off) inside the injection tower.

Installation, Operation, and Maintenance Part 3, Operation

ALS Configuration 6

6850 Series GC

Installation, Operati Part 3, Operation

Use your Agilent data system to configure the injector for use.

Inject modeSelect the injection mode.

Normal-Injects a single sample (including large volume injections with only one septum puncture).

Sample + L2-Injects a mixture of two samples separated by an air gap.

Sample + L2 + L3-Injects a mixture of three samples separated by air gaps.

Large Volume-Performs large volume injections using multiple septum punctures (multiple injections per run).

Wash mode This is only displayed when the injector has a transfer turret installed.

Wash using A, BUses solvent bottle A if injector uses solvent A washes and solvent bottle B if injector uses solvent B washes.

Use A-A2, B-B2Uses solvent bottles A and A2 if injector uses solvent A washes and solvent bottles B and B2 if injector uses solvent B washes. The injector alternates between both bottles.

Use All A, BUses all 6 solvent A bottles if injector uses solvent A washes. Uses all 4 solvent B bottles if injector uses solvent B washes. The injector alternates between all bottles.

Solvent SavingUse this option to vary the syringe wash amount from about 10% to 80% of syringe volume. This setting reduces the amount of solvent and sample consumed during each injection cycle. See "Sample Carryover" on page 142 for more information.

Syringe sizeEnter a syringe size between 1 and 500 L.

NOTE The GC assumes the syringe volume represents full plunger stroke. If the maximum volume marked on the syringe barrel is halfway up the barrel, you will need to enter double that volume (the label in this case is half of the full plunger stroke).

Injector LightControl the LED light (On, Off) inside the injection tower.

on, and Maintenance 151

6 ALS Configuration

5975T LTM-GC/MSD

152

Configure the injector using your Agilent data system. Refer to your data system help for details.

Installation, Operation, and Maintenance Part 3, Operation

ALS Configuration 6

Configuring the Sample Tray

7890 Series GC and 7820 MSD

Installation, Operati Part 3, Operation

The sample tray delivers sample vials to the front and rear injectors according to the defined sequence parameters. There is a separate set of sequence parameters for each injector. The sample tray delivers vials to the front injector before the rear injector. Stored sequences and bar code configurations can be used to tell the sample tray where to deliver and retrieve sample vials.

For the 7890 Series GC, press [Config] [Sample Tray] to access the sample tray and Bar Code Reader setpoints. For the 7820 MSD, press [Config] [Sample Tray] on the GC software keypad to display the sample tray configuration parameters

To edit the sample tray BCR symbology configuration, press [Config] [Sample Tray] > BCR Symbology > [Mode/Status].

CONFIGURE SAMPLE TRAY

BCR symbology Enable All Enable checksum Off

BCR SYMBOLOGY SELECT

Enable All Enable 3 of 9

Enable Code 128

Enable Matrix 2 of 5 Enable Standard 2 of 5

Enable Interleaved 2 of 5 Enable UPC-A

Enable EAN/JAN 13 Enable EAN/JAN 8

Enable UPC-E

on, a

nd Maintenance 153

154

6 ALS Configuration

Press [Info] to view the possible values to enter.

BCR Symbology-Select one of the following bar code setpoints.

Enable AllThe Enable All setting offers the greatest versatility for laboratory use. It can encode both letters and numbers, plus a few punctuation marks, and message length can be varied to suit both the amount of data to be encoded and the space available.

Enable 3 of 9The 3 of 9 setting can encode both letters and numbers, plus a few punctuation marks, and message length can be varied to suit both the amount of data to be encoded and the space available.

Enable Code 128The code 128 setting is used extensively worldwide. It offers a full ASCII 128 symbol set with very dense code. The total character set (14 if alphanumeric, 28 if numeric with starting code C) includes a checksum digit.

Enable Interleaved 2 of 5The 2 of 5 code is restricted to numbers but does allow variable message length.

Enable UPC-AThe Universal Product Code (UPC) is probably the most well-known code in use today. UPC-A codes are 12 numbers (1 number system, 10 data and 1 checksum) and have fixed message length.

Enable UPC-EThe Universal Product Code (UPC) is probably the most well-known code in use today. UPC-E codes are 6 numbers (6 data) and have fixed message length.

Enable EAN/JAN 13The EAN/JAN 13 code offers 13 numbers (2 country, 10 data, 1 checksum).

Enable EAN/JAN 8The EAN/JAN 8 code offers 8 numbers (2 country, 5 data, 1 checksum).

For more information on the Bar Code Reader, see "G4515A Bar Code Reader/Mixer/Heater" on page 25, "Installing the G4515A Bar Code Reader/Mixer/Heater" on page 105, or its Operating Manual.

Enable checksum-Select one of the following checksum setpoints.

OFF-Disables the BCR checksum.

ON-Enables BCR checksum for 3 of 9 and 2 of 5.

Enable Tray Chiller-If installed, enable or disable the heating and cooling plate temperature by turning it On or Off.

Installation, Operation, and Maintenance Part 3, Operation

ALS Configuration 6

Installation, Operati Part 3, Operation

Tray Chiller Temp-If installed, the current heating and cooling plate temperature is displayed. Sets the target heating and cooling plate temperature.

Tray Chiller Error-band-If installed, set the allowable readiness of detection of the temperature error band for the heating and cooling plate temperature before it is declared not ready.

6890 Series GC

The sample tray delivers sample vials to the front and rear injectors according to the defined sequence parameters. There are a separate set of sequence parameters for each injector. The sample tray delivers vials to the front injector before the rear injector. Stored sequences and bar code configurations can be used to tell the sample tray where to deliver and retrieve sample vials.

Press [Config] [Sample Tray] to access the sample tray, Bar Code Reader, and heating and cooling plate setpoints.

CONFIG SAMPLE TRAY

Use chiller rdy Off BCR mode All Enable checksum Off

on, a

nd Maintenance 155

156

6 ALS Configuration

To edit the sample tray BCR symbology mode, press [Config] [Sample Tray] > BCR mode > [Mode/Status].

Press [Info] to view the possible values to enter.

Use chiller rdy-If installed, enable or disable detection of the heating and cooling plate temperature readiness. This feature is only available for the 6890N GC.

For 6890A and 6890 Plus GCs, the chiller/heater temperature must be manually monitored to determine readiness before running samples.

Chiller err band-If the heating and cooling plate readiness detection is enabled, set the allowable error band for the heating and cooling plate temperature before it is declared not ready.

BCR mode-Select one of the following bar code setpoints.

All symbologiesThe All symbologies setting offers the greatest versatility for laboratory use. It can encode both letters and numbers, plus a few punctuation marks, and message length can be varied to suit both the amount of data to be encoded and the space available.

3 of 9The 3 of 9 setting can encode both letters and numbers, plus a few punctuation marks, and message length can be varied to suit both the amount of data to be encoded and the space available.

BCR SYMBOLOGY MODE

All symbologies 3 of 9

Code 128

Standard 2 of 5 Matrix 2 of 5

Interleaved 2 of 5 UPC-A UPC-E

EAN/JAN 8 EAN/JAN 13

Installation, Operation, and Maintenance Part 3, Operation

ALS Configuration 6

Installation, Operati Part 3, Operation

Code 128The code 128 setting is used extensively worldwide. It offers a full ASCII 128 symbol set with very dense code. The total character set (14 if alphanumeric, 28 if numeric with starting code C) includes a checksum digit.

Interleaved 2 of 5The 2 of 5 code is restricted to numbers but does allow variable message length.

UPC-AThe Universal Product Code (UPC) is probably the most well-known code in use today. UPC-A codes are 12 numbers (1 number system, 10 data and 1 checksum) and have fixed message length.

UPC-EThe Universal Product Code (UPC) is probably the most well-known code in use today. UPC-E codes are 6 numbers (6 data) and have fixed message length.

EAN/JAN 8The EAN/JAN 8 code offers 8 numbers (2 country, 5 data, 1 checksum).

EAN/JAN 13The EAN/JAN 13 code offers 13 numbers (2 country, 10 data, 1 checksum).

For more information on the Bar Code Reader, see "G4515A Bar Code Reader/Mixer/Heater" on page 25, "Installing the G4515A Bar Code Reader/Mixer/Heater" on page 105, or its Operating Manual.

Enable checksum-Enable or disable the checksum feature (On, Off).

on, and Maintenance 157

6 ALS Configuration

Configure the ALS on an Intuvo 9000 GC

158

Configure the injector and tray (if installed). Select the syringe size and wash mode as applicable. (The syringe supplied by Agilent with an injector is typically 10 L.)

Installation, Operation, and Maintenance Part 3, Operation

ALS Configuration 6

Installation, Operati Part 3, Operation

For descriptions of the injector solvent wash modes, see "Configuring the Injector" on page 148. For descriptions of the available barcode symbologies, see"Configuring the Sample Tray" on page 153.

on, and Maintenance 159

160

6 ALS Configuration

Installation, Operation, and Maintenance Part 3, Operation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

7 ALS Parameters

Setting the Injector Parameters 162

7890 Series GC, 7820A GC, and 7820 MSD 163

Intuvo 9000 GC 165

6890 Series GC 166

6850 Series GC 168

5975T LTM-GC/MSD 168

Setting the Sample Tray Parameters 169

7890 Series GC and 7820 MSD 169

Intuvo 9000 GC 170

6890 Series GC 170

This chapter describes how to set the automatic liquid sampler parameters using different controlling devices.

The descriptions in this chapter refer to the features available with the GC firmware and does not necessarily describe the capabilities available with Agilent data systems. Refer to Agilent data system online help.

161Agilent Technologies

7 ALS Parameters

Setting the Injector Parameters

162

The available parameters depend on the specific configuration of your GC and injector. Press the [Info] key to view the possible setpoint ranges for each parameter. Refer to Table 7 on page 139 and Table 6 on page 138 for ALS system capabilities.

Installation, Operation, and Maintenance Part 3, Operation

ALS Parameters 7

7890 Series GC, 7820A GC, and 7820 MSD

Installation, Operati Part 3, Operation

For a 7890 Series GC, press [Front Injector] or [Back Injector] on the GC keypad. For a 7820A GC and 7820 MSD, press [Injector] on the software keypad. Scroll to the desired setpoint detailed below.

Enter a setpoint value, use [Mode/Type] to change the selection, or turn the setpoint on or off.

Injection volumeSample volume to be injected. Enter the injection volume in L up to 50% of the configured syringe size. (Using a 10 L syringe, entries would be 0.1, 0.2, 0.3, and so forth up to 5 L.) The GC will round the volume to the next allowable parameter.

FRONT INJECTOR

Injection volume 1.00 Viscosity delay 0 Inject Dispense Speed 6000

Airgap Volume 0.20 Sample Pumps 6 Sample Washes 2 Sample Wash Volume 8 Solvent A post washes 0 Solvent A pre washes 0 Solvent A wash volume 8 Solvent B post washes 0 Solvent B pre washes 0 Solvent B wash volume 8 Sample Draw Speed 300 Sample Dispense Speed 6000 Pre dwell time 0 Post dwell 0 Sample offset 0 Injection Mode NORMAL Tower LED ON

on, a

nd Maintenance 163

164

7 ALS Parameters

Viscosity delayHow many seconds the plunger pauses at the top of the pump and injection strokes to wait for the syringe to fill. For viscous samples, the pause allows the sample to flow into the vacuum created in the syringe.

Inject Dispense SpeedHow many microliters per minute to inject. Parameter value entered here rounds to the nearest acceptable parameter value. For example, 7000 L/min rounds to 7009 L/min.

Airgap VolumeThe amount of air separating the sample from the end of the needle.

Sample pumpsHow many times the syringe plunger is moved up and down with the needle in the sample to expel air bubbles and improve reproducibility.

Sample washesHow many times the syringe is rinsed with sample before the injection. The injector lowers the syringe needle into the sample vial, draws up sample, and empties it into one of the waste bottles.

Sample Wash VolumeHow many microliters used for the sample wash.

Solvent A post washesHow many times the syringe is rinsed with solvent from the solvent A (A1 through A6) bottles.

Solvent A pre washesHow many times the syringe is rinsed with solvent from the solvent A (A1 through A6) bottles.

Solvent A wash volumeHow many microliters used for the sample A wash.

Solvent B post washesHow many times the syringe is rinsed with solvent from the solvent B (B1 through B4) bottles.

Solvent B pre washesHow many times the syringe is rinsed with solvent from the solvent B (B1 through B4) bottles.

Solvent B wash volumeHow many microliters used for the sample B wash.

Sample Draw SpeedThe speed of the syringe plunger during sample injection.

Sample Dispense SpeedIf using a variable plunger speed, the speed of the sample injection.

Solvent Draw SpeedIf using a variable plunger speed, the speed of the syringe plunger during solvent injection.

Installation, Operation, and Maintenance Part 3, Operation

ALS Parameters 7

Installation, Operati Part 3, Operation

Solvent Dispense SpeedIf using a variable plunger speed, the speed of the solvent injection.

Pre dwell timeHow long, in minutes, the needle remains in the inlet before the injection.

Post dwellHow long, in minutes, the needle remains in the inlet after the injection.

Sample offsetEnables variable sampling depth.

Injection modeThe type of injection mode.

Injection RepsIf Injection mode is LVI with multiple reps, the amount of repetitions.

Injection DelayIf Injection mode is LVI with multiple reps, the length of delay between repetitions.

L2 volumeIf Injection mode is a 2-layer or 3-layer Sandwich Injection, the amount of sample used in layer 2.

L2 Airgap volumeIf Injection mode is a 2-layer or 3-layer Sandwich Injection, the amount of air used between sample layer 1 and sample layer 2.

L3 volumeIf Injection mode is a 3-layer Sandwich Injection, the amount of sample used in layer 3.

L3 Airgap volumeIf Injection mode is a 3-layer Sandwich Injection, the amount of air used between sample layer 2 and sample layer 3.

Tower LEDControl the LED light inside the injection tower.

Intuvo 9000 GC

When mounted on an Intuvo 9000 GC, the injector has the same capabilities as on the 7890 Series GC. Set the parameters using the Agilent data system.

on, and Maintenance 165

7 ALS Parameters

6890 Series GC

166

Press [Front Injector] or [Back Injector].

Scroll to the desired setpoint detailed below.

Enter a setpoint value, use the [Mode/Type] key, or turn the setpoint on or off.

Depending on the Inject mode set during injector configuration, the following parameters may be available:

Injection volume/Sample volumeSample volume to be injected. Enter the injection volume in L up to 50% of the configured syringe size. (Using a 10 L syringe, entries would be 0.1, 0.2, 0.3, and so forth up to 5 L.) The GC will round the volume to the next allowable parameter.

Set the injection volume to Off to disable the injector tower.

L2 volumeSample volume to be injected for sample layer 2.

L3 volumeSample volume to be injected for sample layer 3.

#Injection repsFor Large Volume injections. How many times the syringe plunger is moved up and down with the needle in the sample to expel air bubbles and improve reproducibility.

FRONT INJECTOR

Injection vol 1.00 #Sample pumps 6 Air gap 0.20

Viscosity delay 0 #Sample washes 0 #Solv A washes 0 #Solv B washes 0 Plunger speed FAST Pre dwell time 0.00 Post dwell 0.00 Samp offset Off #Solv A pre wash 0 #Solv B pre wash 0

Installation, Operation, and Maintenance Part 3, Operation

ALS Parameters 7

Installation, Operati Part 3, Operation

Injection delayFor Large Volume injections. How many seconds the plunger pauses at the top of the pump and injection strokes. For viscous samples, the pause allows the sample to flow into the vacuum created in the syringe.

#Sample pumpsHow many times the syringe plunger is moved up and down with the needle in the sample to expel air bubbles and improve reproducibility.

#L2 pumpsFor 2-sample layer injections. How many times the syringe plunger is moved up and down with the needle in the sample to expel air bubbles and improve reproducibility.

#L3 pumpsFor 3-sample layer injections. How many times the syringe plunger is moved up and down with the needle in the sample to expel air bubbles and improve reproducibility.

Air gapThe amount of air drawn after the sample.

L2 air gapThe amount of air drawn after sample layer 2.

L3 air gapThe amount of air drawn after sample layer 3.

Viscosity delayHow many seconds the plunger pauses at the top of the pump and injection strokes. For viscous samples, the pause allows the sample to flow into the vacuum created in the syringe.

#Sample washesHow many times the syringe is rinsed with sample before the injection. The injector lowers the syringe needle into the sample vial, draws up sample, and empties it into one of the waste bottles.

#Solvent A washesHow many times the syringe is rinsed with solvent from the solvent A (A, A2, A3) bottles.

#Solvent B washesHow many times the syringe is rinsed with solvent from the solvent B (B, B2, B3) bottles.

Plunger speedThe speed of the syringe plunger during injection.

Pre dwell timeHow long, in minutes, the needle remains in the inlet before the injection.

Post dwellHow long, in minutes, the needle remains in the inlet after the injection.

Samp offsetEnables variable sampling depth. Off disables the setpoint.

on, and Maintenance 167

168

7 ALS Parameters

#Solvent A prewashHow many times the syringe is rinsed with solvent before loading the syringe.

#Solvent B prewashHow many times the syringe is rinsed with solvent before loading the syringe.

6850 Series GC

Set all parameters from your Agilent data system. Refer to your data system help for details.

5975T LTM-GC/MSD

Set all parameters from your Agilent data system. Refer to your data system help for details.

Installation, Operation, and Maintenance Part 3, Operation

ALS Parameters 7

Setting the Sample Tray Parameters

Installation, Operati Part 3, Operation

The available parameters depend on the specific configuration of your GC and sample tray. Press the [Info] key to view the possible setpoint ranges for each parameter. Refer to Table 7 on page 139 and Table 6 on page 138 for ALS system capabilities.

7890 Series GC and 7820 MSD

Press [Sample Tray].

Scroll to the desired setpoint detailed below.

Enter a setpoint value, use [Mode/Type] to change the selection, or turn the setpoint on or off.

Press [Info] to view the possible values to enter.

Enable barcode-If installed, enable or disable the Bar Code Reader by choosing On or Off.

Enable vial heater-If a BCR is installed, enable or disable the vial heater by choosing On or Off.

Vial heater temp-If the vial heater is enabled, set the vial heating temperature.

Vial heater time-If the vial heater is enabled, set the vial heating time.

Enable mixer-If a BCR is installed, enable or disable the mixer by choosing On or Off.

SAMPLE TRAY

Enable barcode Off Enable vial heater Off Enable mixer On

Mixing cycles 4 Mixing cycle time 1 Mixing speed 4000

on, a

nd Maintenance 169

170

7 ALS Parameters

Mixing cycles-If the mixer is enabled, set the number of mixing cycles.

Mixing cycle time-If the mixer is enabled, set the mixing cycle time in seconds.

Mixing speed-If the mixer is enabled, set the mixing speed in revolutions per minute.

Intuvo 9000 GC

When mounted on an Intuvo 9000 GC, the tray and BCD have the same capabilities as on the 7890 Series GC. Set the parameters using the data system.

6890 Series GC

The sample tray delivers sample vials to the front and rear injectors according to the defined sequence parameters. There are a separate set of sequence parameters for each injector. The sample tray delivers vials to the front injector before the rear injector. Stored sequences and bar code configurations can be used to tell the sample tray where to deliver and retrieve sample vials.

Press [Sample Tray].

Scroll to the desired setpoint detailed below.

Enter a setpoint value, use the [Mode/Type] key, or turn the setpoint on or off.

Enable TrayTurn On for a tray sequence, Off for sample bottles in the injector turret.

Tray tempAvailable if the heating and cooling plate is installed. Displays the actual tray temperature.

SAMPLE TRAY

Enable tray On Enable barcode On

BCR temp 28 Off Mix cycles Off

Installation, Operation, and Maintenance Part 3, Operation

ALS Parameters 7

Installation, Operati Part 3, Operation

Tray temp targetAvailable if the heating and cooling plate is installed. Set the tray target temperature.

Enable barcodeAvailable if the Bar Code Reader is installed. Turns the Bar Code Reader on or off.

BCR tempDisplays the actual BCR heater temperature and defines the BCR heater temperature setpoint.

Mix cyclesDefines the number of mix cycles.

For more information on the Bar Code Reader, see "G4515A Bar Code Reader/Mixer/Heater" on page 25, "Installing the G4515A Bar Code Reader/Mixer/Heater" on page 105, or its Operating Manual.

on, and Maintenance 171

172

7 ALS Parameters

Installation, Operation, and Maintenance Part 3, Operation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

8 Syringes and Needles

Selecting a Syringe 174

Inspecting a Syringe 177

Installing a Syringe 178

Removing a Syringe 182

Replacing a Syringe Needle 183

The injector uses syringes as the sample-handling device. This chapter describes their properties and uses.

173Agilent Technologies

8 Syringes and Needles

Selecting a Syringe

174

1 Select the syringe type based on the inlet you are using and the volume of sample you want to inject.

CAUTION Failure to use an on-column syringe when injecting into an on-column inlet could damage the injector, syringe and column.

2 Select a syringe. Refer to the Agilent catalog for consumables and supplies for part numbers and ordering information.

3 Select the appropriate syringe needle gauge (Table 9).

Table 9 Needle gauge selection

Inlet type Column type Needle gauge

Purged-packed, split or splitless (including MMI and PTV)

Any Applicable

23 gauge 26s gauge or 23/26s gauge tapered

Cool on-column 250 m 320 m 530 m

26s/32 gauge tapered 26s/32 gauge tapered 23/26s gauge tapered or 26s gauge

Installation, Operation, and Maintenance Part 3, Operation

Syringes and Needles 8

Installation, Operati Part 3, Operation

Use syringe needles with a conical tip. Do not use sharp-tipped needles. They tear the inlet septum and cause leaks. Also, a sharp-tipped needle tends to wipe off on the septum as it exits resulting in a large solvent tail on the chromatogram (Figure 42 and Figure 43).

Table 10 On-column autosampler syringes

Volume (L) Description Unit Part No.

5 Removable needle, barrel only 5182-0836

Stainless steel needle for 530 m column 3/pk 5182-0832

Stainless steel needle for 0.32 mm column 3/pk 5182-0831

Stainless steel needle for 0.25 mm column 3/pk 5182-0833

Plunger button 10/pk 5181-8866

Figure 42 Needle tips

Sharp tip Cone tip

on, and Maintenance 175

176

8 Syringes and Needles

Figure 43 Needle shapes

5.04.54.03.53.02.52.01.51.00.50.0

Needle tips

Tapered needle

Installation, Operation, and Maintenance Part 3, Operation

Syringes and Needles 8

Inspecting a Syringe

Installation, Operati Part 3, Operation

Before installing a syringe:

1 Roll the syringe on the edge of a clean flat surface. If the tip of the needle moves in a circle, straighten the shaft by bending it slightly near where it connects to the syringe barrel and check it again (Figure 44).

2 Check for a rough needle. The needle surface may have closely spaced concentric ridges that act like a miniature file and abrade pieces of the septum into the inlet or vial. The ridges are easy to see under 10X magnification.

If there are ridges, polish the needle by pulling it through a folded piece of fine emery paper between your finger and thumb until the ridges are gone. Be careful not to modify the tip of the syringe.

3 Check for a sticky plunger. Slide the plunger of the syringe up and down a few times. It should move smoothly without sticking or binding. If it is sticky, remove the plunger and clean it with solvent.

Figure 44 Inspecting the syringe

Bend here if necessary

on, and Maintenance 177

8 Syringes and Needles

Installing a Syringe

178

To install a syringe (Figure 45):

1 Unplug the injector cable, and if desired, mount the injector on a parking post, or lay the injector tower on a work bench.

2 Open the injector door.

3 Slide the syringe carriage to the top position.

4 Open the syringe latch by swinging it in a counterclockwise direction.

5 Lift the plunger carrier to the top position.

6 Carefully pass the syringe needle through the guide hole in the needle support foot.

Figure 45 Installing a syringe

Plunger carrier

Plunger screw

Flange guide

Syringe carrier

Syringe latch

Needle support foot

Slide

Plunger

Flange

Needle

Barrel

Installation, Operation, and Maintenance Part 3, Operation

Syringes and Needles 8

Installation, Operati Part 3, Operation

7 Align the syringe flange with the flange guide and press the syringe into place, keeping the needle end in the guide hole of the needle support foot. Make sure that the flat edge of the syringe flange faces out (Figure 46).

NOTE Failure to correctly install the syringe flange into the flange guide will result in damage to the syringe plunger.

8 Close the syringe latch by swinging it clockwise until it snaps in place.

9 Loosen the plunger screw entirely by turning it counterclockwise until the stop is reached.

10 Slide the plunger carrier down until it is completely over the syringe plunger, and tighten the plunger thumb screw until finger-tight.

11 Manually move the plunger carrier up and down. If the syringe plunger does not move along with the carrier, repeat the previous steps until installed correctly. Be sure the plunger thumb screw is secure and tight. If the carrier is not completely attached to the syringe plunger it may become detached after a few injections.

Figure 46 Syringe flange orientation

Flange

Flange guide

on, and Maintenance 179

180

8 Syringes and Needles

CAUTION Repeating this movement can damage the syringe.

12 Verify that the needle is inside the guide hole of the needle support foot. The needle should be straight and pass freely through the needle guide hole.

If the needle is bent or is outside the guide hole, remove the syringe and reinstall. See Figure 47 for a properly installed syringe.

13 Close the injector door.

Figure 47 Syringe carriage and needle support with syringe installed

Syringe latch (closed)

Needle support foot

Installation, Operation, and Maintenance Part 3, Operation

Syringes and Needles 8

Installation, Operati Part 3, Operation

14 Do the following only if the injector tower was removed from the mounting post during installation:

a If necessary, plug in the injector cable.

b Install the injector on the mounting post. See Installing the G4513A Injector" on page 58 for details.

c If you have a sample tray, calibrate the ALS system. See Calibrating the ALS System" on page 249 for details.

on, and Maintenance 181

8 Syringes and Needles

Removing a Syringe

182

To remove a syringe:

1 Unplug the injector cable, and if desired, mount the injector on a parking post.

2 Open the injector door.

3 Slide the syringe carriage to the top position.

4 Completely loosen the plunger thumb screw until it reaches the stop, and lift the plunger carrier off of the syringe plunger.

5 Open the syringe latch by swinging it in a counterclockwise direction.

CAUTION Be careful not to bend the syringe needle. Only pull the syringe out of the carriage until clear. The needle bends easily when still seated in the needle support guide.

6 Carefully pull the top of the syringe out of the flange guide, then lift the needle out of the needle support foot.

To install a syringe, see Installing a Syringe" on page 223 for details.

Installation, Operation, and Maintenance Part 3, Operation

Syringes and Needles 8

Replacing a Syringe Needle

Installation, Operati Part 3, Operation

The stainless steel needles used for 250-m and 320-m injections must be inserted into a glass syringe barrel. Select the correct size needle for the column you will use.

Needles for 250-m injections have silver-colored stops. Needles for 320-m injections have gold-colored stops. See your Agilent consumables and supplies catalog or the Agilent web site (www.agilent.com/chem) for a list of syringes and needles.

Table 11 On-column autosampler syringes

Volume (L) Description Unit Part No.

5 Removable needle, barrel only 5182-0836

Stainless steel needle for 530 m column 3/pk 5182-0832

Stainless steel needle for 320 m column 3/pk 5182-0831

Stainless steel needle for 250 m column 3/pk 5182-0833

Plunger button 10/pk 5181-8866

on, and Maintenance 183

184

8 Syringes and Needles

To insert a needle into a syringe barrel (Figure 48):

1 Unscrew the syringe barrel cap and remove the spring.

2 Make sure the needle has a PTFE disk (Figure 48). If the syringe barrel does not have the PTFE disk, use the instructions in the syringe box to wrap the needle yourself.

3 Slide the spring and the cap down over the needle.

4 Insert the needle into the syringe barrel.

5 Screw the cap back on the syringe barrel.

Figure 48 Syringe parts

Plunger

Barrel

PTFE disk

Needle

Spring

Cap

PTFE disk

Stop

Needle

Installation, Operation, and Maintenance Part 3, Operation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

9 Vials and Bottles

Preparing a Sample Vial 186

Select a sample vial 186

Select a vial septa 187

Fill a sample vial 189

Label a sample vial 188

Cap a sample vial 190

Preparing Solvent and Waste Bottles 192

Select the bottles 192

Fill the solvent bottles 193

Prepare the waste bottles 193

Placing Vials and Bottles in the Turret 194

With a sample tray 195

Without a sample tray 196

Using Two Injectors (7890A and 6890 Series GCs Only) 197

With a sample tray 197

Without a sample tray 197

How Many Sample Vials Can I Run? 198

Sandwich Injections 204

Example 2-layer sandwich injection 206

Example 3-layer sandwich injection 208

This chapter describes sample vials, solvent bottles, and waste bottles, including how to place them in the tray or turret. It also discusses how to estimate the maximum number of samples that can be run before solvent bottles must be refilled or waste bottles must be emptied.

185Agilent Technologies

9 Vials and Bottles

Preparing a Sample Vial

Select a sample vial

186

The injector and sample tray use clear or amber glass sample vials with crimp caps, or screw-cap vials. Use amber glass vials for light-sensitive samples. Refer to your Agilent catalog for consumables and supplies for acceptable vial types. Incompatible sample vials cause tray and turret errors.

Figure 49 shows the critical dimensions for sample vials used with the 7693A ALS system. These dimensions do not make up a complete set of specifications.

Figure 49 Sample vial dimensions

8.2 0.2

Body Diameter (BD) = 11.7 0.2 Cap Diameter (CD) = BD 1.03 maximum All dimensions in millimeters

3.7 minimum

CD

BD

11.7 0.2

6.25 0.25

25 minimum body height

32 0.5 33.5 1.5

Vial plus cap height

Maximum height of a capped vial Crimp cap sample vial

8.2 .

NOTE The transfer turret does not work with the micro V-vial. The bottom of the micro V-vial can get caught in the turret.

Install

ation, Operation,
and Maintenance Part 3, Operation

Vials and Bottles 9

Select a vial septa

Installation, Operati Part 3, Operation

There are two types of septa used with crimp caps and screw-on caps, each with different resealing characteristics and different resistance to solvents.

One type is natural rubber formulation coated with PTFE on the sample side. This septum is suitable for samples with a pH range of 4.0 to 7.5. They are less resistant to solvents after puncture and are more easily cored than silicone rubber septa. Coring may deposit septum pieces in the vial and affect your chromatograms.

Another is high-quality, low-extractable silicone rubber septa, coated with PTFE on one or both sides. They are more resistant to solvents after puncture and to coring by the needle.

Refer to your Agilent catalog for consumables and supplies for more information.

Figure 50 shows the diameter for vial cap apertures.

Figure 50 Vial cap aperture specifications

5.58 mm (0.220 in.) recommended4.65 mm (0.183 in.) minimum

on, and Maintenance 187

9 Vials and Bottles

Label a sample vial

188

Some vials are available with a write-on spot for easy marking. If you choose to make and apply your own labels, Agilent Technologies recommends the positioning and maximum label thickness shown in Figure 51.

Figure 51 Sample vial label dimensions

3.6 minimum

Overlap mismatch 1.0 maximum

26.6 maximum

13.4 minimum No label or tape

No label or tape

Label skew

16.4 0.5

13.2 maximum Text areas are optional

Text

Bar code

Text

All dimensions are in millimeters Maximum width

measured at overlap

12.56

Correct sample vial dimensions are critical for proper gripper operation. Vials and labels

CAUTION that do not meet these specifications may cause sampler errors. Service calls and repairs found to be due to vials and microvials that do not meet these specifications are not covered under warranty or the service contract.

Do not use glossy labels when using the bar code reader accessory.

NOTE

Installation, Operatio

n, and Maintenance Part 3, Operation

Vials and Bottles 9

Fill a sample vial

Installation, Operati Part 3, Operation

Figure 52 shows the recommended fill volumes for sample vials of:

1 mL for the 2-mL vial

50 L for the 100-L vial

The air space in the vial is necessary to avoid forming a vacuum when sample is withdrawn. This could affect reproducibility.

CAUTION Do not inject air into the vials to prevent this vacuum. This often damages the cap seal and may damage syringe needles.

When developing your method, keep the following in mind:

If you need to test a large amount of sample over repeated injections, divide the sample among several vials to obtain reliable results.

When sample volume in the vial is low, contaminants from the previous sample injection or solvent washes may have a greater impact on the sample.

If you change suppliers, you may need to redevelop your method. Differing manufacturing practices for vial hardware sometimes cause variances in your results.

Figure 52 Recommended fill volumes for sample vials

3.6 mm*

1 mL 50 L

100-L vial2-mL vial

* Needle position based on default sampling depth.

on, and Maintenance 189

9 Vials and Bottles

Cap a sample vial

190

CAUTION If using a sample vial with a screw cap top, be sure to completely tighten the screw cap before use.

To install the airtight crimp caps:

1 Clean the inside surfaces of the crimper jaws.

2 Place the crimp cap over the top of the vial.

3 Lift the vial into the crimper. Squeeze the handle until it reaches the adjuster screw.

Figure 53 Crimping caps

Handle

Adjuster screw

Jaws

Crimp cap

Sample vial

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Installation, Operati Part 3, Operation

Figure 54 shows acceptable and unacceptable vial caps.

Check each vial for proper crimping:

1 Be sure there are no folds or wrinkles on the part of the cap that wraps under the neck of the vial. To remove folds or wrinkles, turn the vial about 10 and crimp it again. Adjust the crimper for a looser crimp by turning the adjusting screw clockwise.

2 The cap should be finger-tight. If the cap is loose, adjust the crimper for a tighter crimp by turning the adjusting screw counterclockwise. Crimp the cap again. If the cap is too tight, the septum will distort and the vial may leak.

3 Be sure that each cap has a flat septum centered over the top of the vial.

If the septum is not flat, remove the cap, turn the crimper adjusting screw clockwise, and try again.

If the cap is not centered, remove the cap and make sure the new cap is flat on the top of the vial before you squeeze the crimper.

Figure 54 Acceptable and unacceptable caps

Centered

No folds or wrinkles

Acceptable

Off-center

Folds and wrinkles

Unacceptable

on, and Maintenance 191

9 Vials and Bottles

Preparing Solvent and Waste Bottles

192

The solvent bottles hold solvent for rinsing the syringe between injections. The injector dispenses the solvent washes and sample washes into waste bottles. The number of samples that can be analyzed may be limited by the wash or waste bottle capacity.

Select the bottles

Solvent and waste bottles can be closed with either diffusion caps (a plastic cap with a hole; it retards evaporation while letting the needle enter freely) or septa. Agilent Technologies recommends diffusion caps (Figure 55) over septa for two reasons:

The diffusion cap allows multiple entrances into a bottle without contaminating the liquid inside the bottle with small pieces of septum material.

For common solvents, the rate of diffusion out of the bottle is less with a diffusion cap than with a septum that has been punctured multiple times with a standard syringe needle.

Figure 55 Four milliliter bottle used for solvent or waste

Diffusion cap (recommended)

Minimum solvent level

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Fill the solvent bottles

Installation, Operati Part 3, Operation

Rinse and fill each solvent bottle with 4 mL of fresh solvent. The liquid level will be near the shoulder of the bottle. Good laboratory practice dictates using no more than 2.0 mL of the 4 mL solvent for syringe washes. The needle tip draws solvent 18.5 mm from the bottom of the vial (Figure 56).

Figure 56 Needle tip position when withdrawing solvent

18.5 mm

4-mL fill volume

2.0 mL usable solvent volume

2-mL solvent remains

MINIMUM SOLVENT LEVEL

Prepare the waste bottles

Empty and rinse each waste bottle after each multiple vial run. The syringe can discard about 4 mL of waste into the waste bottle (Figure 57).

Figure 57 Needle tip position when discharging waste

4-mL maximum waste

on, and Maintenance 193

9 Vials and Bottles

Placing Vials and Bottles in the Turret

194

Two turrets are provided with the G4513A injector (Figure 58). The 16-sample standalone turret, and the 3-sample transfer turret.

The labeled positions are defined in Table 12 and Table 13.

Figure 58 Injector turrets

Table 12 Standalone turret labels

Position Label Bottle/Vial

1 through 14 1 through 14 Sample vials

15 15 L2

Sample vial Layer 2 bottle

16 16 L3

Sample vial Layer 3 bottle

17 W Waste bottle

18 B Solvent B bottle

19 A Solvent A bottle

Transfer turret (3 samples)

Standalone turret (16 samples)

A B

16

3

1

5

7

9 12

14

Top views.

W

15

13

11 10 8

6

4

2 A

A2

A4 L3

L1

WB

WA

WA B2

A6

A3

A5

B

B3 B4 WA

WB

L2

L3

L2

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Installation, Operati Part 3, Operation

Table 13 Transfer turret labels

Position Label Bottle/Vial

1 L1 Dedicated vial transfer position A Layer 1 bottle

2 L2 Configurable vial transfer position B Layer 2 bottle

3 L3 Configurable vial transfer position C Layer 3 bottle

4 and 5 WB Waste B1 - B2 bottles

6 through 8 WA Waste A1 - A3 bottles

9 through 12 B - B4 Solvent B1 - B4 bottles

13 through 18 A - A6 Solvent A1 - A6 bottles

With a sample tray

You must use the transfer turret. Place up to 150 samples in the three vial racks, according to the programmed sequence. Figure 59 shows tray loading for a sequence that uses tray positions 6 through 21.

Figure 59 Loading tray positions

First vial in position 6

Last vial in position 21

150

141 1

10

21

110

101

100

91

60

51

50

41

6

8 9

7

11

20

12 13 14

16

18 19

15

17

on, and Maintenance 195

9 Vials and Bottles

Without a sample tray

196

You can place up to sixteen vials, a solvent A, a solvent B, and a waste bottle in the standalone turret. If you use the transfer turret, you can load three sample vials and the solvent and waste bottle capacity increases to 15 (Figure 58).

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Using Two Injectors (7890A and 6890 Series GCs Only)

Installation, Operati Part 3, Operation

With two injectors mounted on the GC, you can use the front injector, the back injector, or both injectors simultaneously.

Two-injector configurations have the following characteristics:

The system sends one injector ready signal to the GC when both injectors are ready and one start signal at the beginning of the injection stroke.

The two injectors inject simultaneously.

With a sample tray

Set run parameters for each injector in the sequence. When you set up the run parameters, you must identify which data channel is for which injector on a 7890A or 6890 Series GC.

When using a 7890A or 6890 Series GC for sampler control, you assign the channel when you set up the sequence.

When using the Agilent data system, refer to its online help when defining the instrument channels.

When both injectors have samples, they begin the injection cycle together, including any sample washes. After they have completed the specified sampling steps, they inject the samples. After the injection, both injectors go through the solvent wash cycle. When both injectors are done, the tray picks up the sample vial from the front injector and returns it to its original position on the tray, then picks up the vial from the back injector and returns it to its original position.

Without a sample tray

The injectors go through the same motions as with a sample tray. The injectors inject samples from position 1 first, followed by samples from the remaining sample vial positions. If the two injectors do not have the same number of samples, the injector that completes its sequence first remains idle while the other finishes.

on, and Maintenance 197

9 Vials and Bottles

How Many Sample Vials Can I Run?

198

The number of sample vials that you can run at one time is determined by the following parameters for your application:

The number of sample injections per sample vial.

The syringe size used: 1 L to 500 L.

The syringe wash volume (Table 14)

The number of solvent washes (both pre- and post-injection) required from each solvent bottle.

The number of sample washes and solvent washes per sample injection that the injector discards into each waste bottle.

Number of waste bottles.

Table 14 Syringe wash volumes

Syringe Size (L)

80% syringe volume L

40% syringe volume L

30% syringe volume L

20% syringe volume L

10% syringe volume L

1 0.8 0.4 0.3 0.2 0.1

2 1.6 0.8 0.6 0.4 0.2

5 4 2 1.5 1 0.5

10 8 4 3 2 1

25 20 10 7.5 5 2.5

50 40 20 15 10 5

100 80 40 30 20 10

250 200 100 75 50 25

500 400 200 150 100 50

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Solvent bottle equation (injector)

Installation, Operati Part 3, Operation

CAUTION The number of sample vials given by these equations are estimates. Solvent characteristics such as evaporation rate and surface tension may affect the capacity of the bottles.

This equation estimates the maximum number of sample vials you can run from one solvent bottle.

Maximum number of sample vials per solvent bottle

where:

VW = Volume of Wash in L taken from Table 14

NSI = Number of sample injections taken from each sample vial

NSW = Number of Solvent washes (pre and post) per sample injected

= 2000

VW x NSI x NSW

Waste bottle equation

This equation estimates the maximum number of sample vials you can run per one waste vial type (A orB).

Maximum number of sample vials per waste bottles used (WA or WB)

where:

VWaste = Volume of waste bottles used in L. Use 4000.

= VWaste

WWash x NSI x NSS

x W

on, and Maintenance

199

200

9 Vials and Bottles

VWash = Volume of wash in L taken from Table 14

NSI = Number of sample injections taken from each sample vial

NSS = Total number of pre- and post-solvent A or solvent B washes and sample washes per sample injected. When using both solvent A and B, the sample wash is divided evenly between all four waste bottles in the 1-sample turret

W = Waste vial usage.

Standalone, W = 1 Transfer, W = 3 for waste A, W = 2 for waste B

Example

A tray is installed with a 3-sample transfer turret in the injector. Your application parameters are:

Two injections per vial

Three washes from solvent bottle A

Two washes from solvent bottle B

Two sample washes

10-L syringe

80% wash volume (default)

1 Substitute the parameters of your application into the solvent bottle equation:

For Solvent A

VW = 8 from Table 14 NSI = 2 NSW = 3

Maximum number of sample vials per solvent bottle

= 2000

8 x 2 x 3 = 41

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Installation, Operati Part 3, Operation

For Solvent B

VW = 8 from Table 14 NSI = 2 NSW = 2

Maximum number of sample vials per solvent bottle

2 Substitute the parameters of your application into the waste bottle equation:

For Waste A

VWaste = Use 12000 for the 3-sample transfer turret VWash = 8 from Table 14 NSI = 2 NSS = 3 (solvent A washes) + 1 (Sample wash) = 4

Maximum number of sample vials per waste bottles used (WA)

For Waste B

VWaste = Use 8000 for the 3-sample transfer turret VWash = 8 from Table 14 NSI = 2 NSS = 2 (solvent B washes) + 1 (sample wash) = 3

Maximum number of sample vials per waste bottles used (WB)

= 2000

8 x 2 x 2 = 62

= 12000

8 x 2 x 4 = 187.5

on, and Maintenance 201

202

9 Vials and Bottles

3 Analyze the results keeping the following in mind:

The 3-sample transfer turret has 3 waste A bottles and 2 waste B bottles. If solvent A is used, all waste A bottles must be used. If solvent B is used, all waste B bottles must be used.

Sample washes are evenly distributed to all waste bottles used.

The injector can be configured to use 1 A, 1 B; 2 As, 2 Bs; or all 6 As and 4 Bs.

Using a single solvent bottle of each type, all five waste bottles must be used; 41 vials can be run. Using two solvent A bottles and two solvent B bottles, 84 vials can be run. If you use three solvent A bottles and three solvent B bottles, you can run 150 vials which is the tray capacity limit.

= 8000

8 x 2 x 3 = 166

We strongly recommend that all waste positions in all turrets contain bottles at all

CAUTION times, regardless of which ones are actually used in the current configuration. This protects against accidental discharge of solvent into the turret body, which could damage it.

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Reduced solvent and sample usage

Installation, Operati Part 3, Operation

Solvent and sample washes by default use 80% of the syringe capacity. Solvent and sample can be conserved by reducing this volume. This is done by configuring each injectors solvent saving setting.

CAUTION PTFE-tipped syringes must be used to lubricate the barrel wall. Standard syringes, will fail quickly with limited lubrication.

The solvent wash can be set to use less than the usual 80%. This modifies the solvent wash process as follows:

1 The syringe draws in solvent to the percent specified, which can be as little as 10% of the syringe size.

2 The syringe and needle rise out of the solvent bottle.

3 The plunger rises to the 80% mark, rinsing the syringe barrel with solvent, followed by air.

4 The solvent and air are discharged into a waste bottle.

Confirm that sample carryover (see Sample Carryover) is not a problem with the reduced solvent washes.

on, and Maintenance 203

9 Vials and Bottles

Sandwich Injections

204

The ALS system has the ability to draw liquid from multiple vials to create multilayered, or sandwich, injections. Each layer of sample, internal standard, or solvent can be separated by a layer of air (from 0% to 10% of the syringe size). Figure 60 and Figure 61 show examples of 2-and 3-layer sandwich injections.

Figure 60 2-layer sandwich injection

Figure 61 3-layer sandwich injection

Air gap 1 Air gap 2

Layer 1 (L1) Layer 2 (L2)

Air gap 1 Air gap 2

Layer 1 (L1) Layer 2 (L2)

Air gap 3

Layer 3 (L3)

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Installation, Operati Part 3, Operation

When loading vials for sandwich injections, layer 1 (L1) can be placed in any turret position 1-14 if using a standalone turret, or in any tray position 1-150 if using a transfer turret with sample tray. The vials for layer 2 (L2) and layer 3 (L3) must be placed in turret positions L2 and L3 (Figure 62), respectively.

For the following examples, we assume the use of a 7890 Series GC with a front injector and sample tray. Refer to Setting the Injector Parameters for more information.

Figure 62 Turret vial locations for sandwich injections

Transfer turretStandalone turret

A B

16

3

1

5

7

9 12

14

W

15

13

11 10 8

6

4

2 A

A2

A4 L3

L1

WB

WA

WA B2

A6

A3

A5

B

B3 B4 WA

WB

L2

L3

L2

on, and Maintenance 205

9 Vials and Bottles

Example 2-layer sandwich injection

206

Suppose you want to create a 2-layered sandwich injection using 2.0 L of sample and 15 L of solvent separated by 0.10 L air gaps (Figure 63).

1 Place the sample vial (L1) in tray position 1.

2 Place the solvent vial (L2) in turret position L2.

3 Press [Front Injector] on the GC keypad.

4 Scroll to Injection Mode and select 2-LAYER.

5 Set the following parameters for the front injector:

Injection volume2.0 L

Airgap Volume0.10 L

L2 volume15 L

L2 Airgap Volume0.10 L

Note that for 2- and 3-layer injection modes, Injection volume and Airgap volume represent layer 1 (L1) parameters.

6 Store the method parameters:

a Press [Method] on the GC keypad.

b Scroll to an available method number.

c Press [Store] to select the method number.

d Press [Yes] to confirm.

Figure 63 Example 2-layer sandwich injection with 2.0 L of sample and 15 L of sol- vent

Air gap 1 (0.10 L)

Air gap 2 (0.10 L)

Sample (L1) (2.0 L)

Solvent (L2) (15 L)

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Installation, Operati Part 3, Operation

7 Set up the sandwich injection sequence:

a Press [Seq] on the GC keypad.

b Scroll to Method # under Subseq 1 and press [On/Yes].

c Enter the method number from step 6 using the GC numeric keypad, and press [Enter] to confirm the entry.

d Scroll to Samples and press [1] [.] [1] to set the vial range on the sample tray, and press [Enter] to confirm the entry.

8 Run the sandwich injection sequence:

a Press [Seq control] on the GC keypad.

b Scroll to Start sequence and press [Enter]. The sandwich injection sequence begins.

on, and Maintenance 207

9 Vials and Bottles

Example 3-layer sandwich injection

208

Suppose you want to create a 3-layered sandwich injection using 10 L of solvent A, 2.0 L of sample, and 15 L of solvent B separated by 0.10 L air gaps (Figure 64).

1 Place the solvent A vial (L1) in tray position 1.

2 Place the sample vial (L2) in turret position L2.

3 Place the solvent B vial (L3) in turret position L3. Refer to Figure 62 on page 205 for turret position information.

4 Press [Front Injector] on the GC keypad.

5 Scroll to Injection Mode and select 3-LAYER.

6 Set the following parameters for the front injector:

Injection volume10 L

Airgap Volume0.10 L

L2 volume2 L

L2 Airgap Volume0.10 L

L3 volume15 L

L3 Airgap Volume0.10 L

Note that for 2- and 3-layer injection modes, Injection volume and Airgap volume represent layer 1 (L1) parameters.

Figure 64 Example 3-layer sandwich injection with 10 L of solvent A, 2.0 L of sam- ple, and 15 L of solvent B

Air gap 1 (0.10 L)

Air gap 2 (0.10 L)

Solvent A (L1) (10 L)

Sample (L2) (2.0 L)

Solvent B (L3) (15 L)

Air gap 3 (0.10 L)

Installation, Operation, and Maintenance Part 3, Operation

Vials and Bottles 9

Installation, Operati Part 3, Operation

7 Store the method parameters:

a Press [Method] on the GC keypad.

b Scroll to an available method number.

c Press [Store] to select the method number.

d Press [Yes] to confirm.

8 Set up the sandwich injection sequence:

a Press [Seq] on the GC keypad.

b Scroll to Method # under Subseq 1 and press [On/Yes].

c Enter the method number from step 7 using the GC numeric keypad, and press [Enter] to confirm the entry.

d Scroll to Samples and press [1] [.] [1] to set the vial range on the sample tray, and press [Enter] to confirm the entry.

9 Run the sandwich injection sequence:

a Press [Seq Control] on the GC keypad.

b Scroll to Start sequence and press [Enter]. The sandwich injection sequence begins.

on, and Maintenance 209

210

9 Vials and Bottles

Installation, Operation, and Maintenance Part 3, Operation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

10 Running Samples

Running a Sample 212

Injection volume 212

Using the ALS Controller 213

Sampler response to interruptions 214

Restarting an interrupted sequence 214

Running a Priority Sample 215

This chapter describes the process for running one or more samples.

211Agilent Technologies

10 Running Samples

Running a Sample

WARNING When running a sample, keep your hands away from the syringe needle. The needle is sharp and may contain hazardous chemicals.

212

To operate your automatic liquid sampler:

1 Install a clean syringe. See Installing a Syringe" on page 178.

2 Fill the solvent bottles. See Preparing Solvent and Waste Bottles" on page 192.

3 Place the solvent and waste bottles in the turret. See Placing Vials and Bottles in the Turret" on page 194.

4 Load the sample vials into the turret or the tray. See Preparing a Sample Vial" on page 186.

5 Prepare the GC sequence. See your GC or data system documents.

6 Run the sequence by pressing the start button on the GC. When the GC is ready, the ALS begins the injections.

Injection volume

The injection volume depends on the sample size and the syringe size.

Syringe size may be 1 L, 2 L, 5 L, 10 L, 25 L, 50 L, or 100 L for the standard syringe carriage.

Syringe size may be 250 L or 500 L for the enhanced sample handling syringe carriage.

Sample size may be 1-50% of the syringe size in 1% increments.

Installation, Operation, and Maintenance Part 3, Operation

Running Samples 10

Using the ALS Controller

Installation, Operati Part 3, Operation

When the ALS controller is on a 6890A GC:

[Start] on the G4517A ALS Controller is disabled.

Pressing [Start] on the 6890A GC starts the sequence.

Pressing [Stop] on the G4517A ALS Controller stops the ALS controller sequence, and if the GC is waiting for a signal from the controller to start a run, an error message is displayed on the GC. To resume the sequence, see the following section on Restarting an interrupted sequence" on page 214.

on, and Maintenance 213

10 Running Samples

Interrupting a Run or Sequence

214

The following events interrupt a run:

Power failuresThe power to the GC or controlling device fails.

Stop commands[Stop] on the GC is pressed, or the Stop Run/Abort option is selected from the Agilent data system.

Safety or operator faultsThe sampler recognizes these fault(s):

The injector door was opened

Turret error

Plunger error

Tray arm axis error

The injector was moved on the GC during injection

The tray failed to deliver a vial

Sampler response to interruptions

If the interruption was caused by a problem that the sampler recognizes, a message appears on the GC or Agilent data system. The 6890A, and 6890 Plus GCs do not display sampler messages. For more information, see Error Messages" on page 260.

Power failureAborts the run. Restart the sequence (see below).

Stop commandsInterrupts the run. Restart the sequence (see below).

Safety or operator faultsAborts the run. Restart the sequence (see below).

Restarting an interrupted sequence

To restart an aborted sequence from the point of interruption:

1 Resolve the problem that caused the interruption.

2 The tray automatically moves any unknown vials found in the gripper arms or in an unexpected location to the unknown vials station.

3 The tray automatically starts the run with the next vial in the sequence.

This allows normal operation to continue after an interruption without delaying the entire sequence.

Installation, Operation, and Maintenance Part 3, Operation

Running Samples 10

Running a Priority Sample

Installation, Operati Part 3, Operation

Priority samples are for 7890 and 6890 Series GCs only.

If the Use priority parameter is on in your GC sequence, the sample tray checks for a vial in position 150 before loading a new vial into the injector turret. If it finds a vial there, it loads that sample vial into the turret and runs it according to the method you assigned for priority samples.

When the priority sample run is completed, it returns the priority sample vial to tray position 149, therefore keeping position 150 empty when using this feature. The tray gripper again checks vial position 150 and, if there is no vial there, continues your original sequence from where it was interrupted. For more information on how to program priority sequences, see your 6890 GC documentation.

on, and Maintenance 215

216

10 Running Samples

Installation, Operation, and Maintenance Part 3, Operation

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

Part 4:

Maintenance and Troubleshooting

Maintenance 219

Periodic Maintenance 220

Tray Home and Park Positions 222

Installing a Syringe 223

Removing a Syringe 227

Changing the Turret 228

Adapting for Cool On-Column Injection 232

Replacing the Needle Support Foot 233

Adapting for Syringes Over 100 L 235

Replacing the Syringe Carriage Assembly 236

Replacing a Syringe Needle 243

Aligning the Injector 245

Aligning the Sample Tray 247

Calibrating the ALS System 249

Replacing Power Fuses in the G4517A ALS Controller 252

Faults and Errors 255

Faults 256

Error Messages 260

Troubleshooting 265

Symptom: Variability 266

Symptom: Contamination or Ghost Peaks 268

Symptom: Smaller or Larger Peaks Than Expected 269

Symptom: Sample Carryover 271

Symptom: No Signal/No Peaks 272

Correcting Syringe Problems 273

Correcting Sample Vial Delivery Problems 274

217Agilent Technologies

218

Replacement Parts 275

G4517A ALS Controller (6890A GC Only) 280

G4513A Injector 276

G4514A Sample Tray 278

G4516A ALS Interface Board (6890 Plus GC Only) 282

Installation, Operation, and Maintenance

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

11 Maintenance

Periodic Maintenance 220

Tray Home and Park Positions 222

Installing a Syringe 223

Removing a Syringe 227

Changing the Turret 228

Adapting for Cool On-Column Injection 232

Replacing the Needle Support Foot 233

Adapting for Syringes Over 100 L 235

Replacing the Syringe Carriage Assembly 236

Replacing a Syringe Needle 243

Aligning the Injector 245

Aligning the Sample Tray 247

Calibrating the ALS System 249

Replacing Power Fuses in the G4517A ALS Controller 252

The information in this chapter will assist you in keeping the sampler running smoothly.

219Agilent Technologies

11 Maintenance

Periodic Maintenance

220

This section contains some suggestions for ensuring good performance of your Automatic Liquid Sampler system. The maintenance interval varies with the use of the instrument.

CAUTION Do not use any lubricants on the Automatic Liquid Sampler. They may affect the chemical performance of the GC and damage the instrument.

CAUTION When cleaning the instrument, as described below, use a damp (not wet!), lint-free cloth. Do not use chemical cleaners.

On an occasional basis:

? Calibrate the ALS system. See Calibrating the ALS System on page 249 for details.

? Clean the gantry, tray base, vial racks, turrets, and other surfaces.

? Wipe away any excess spill remains from the spill tray found underneath the turret. See Removing the turret on page 230 for details.

? Clean the needle support foot and nearby surfaces on the injector. Dust and dirt accumulate in these areas and can be picked up by the syringe needle and carried into the inlet.

? Inspect the needle support foot for signs of wear, and replace if necessary. See Replacing the Needle Support Foot on page 233 for details.

? Clean the injector surfaces and door.

? Vacuum any dust found on or around the vents on the injector or tray.

? Make sure the injector mounting post is tight.

? Make sure the tray mounting screws are tight.

? Make sure the mounting bracket screws are tight.

? Be sure all cables are securely connected.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

? If using a liquid heating and cooling plate, the drainage tubing must allow any condensate to drain easily with no back pressure. Make sure that:

The tubing slopes downward towards the drainage container.

The tubing is kept straight without kinks that may block the flow.

The open end of the tubing is not submersed in the drainage container.

The tubing does not become clogged or dirty. Replace tubing if necessary.

on, and Maintenance 221 and Troubleshooting

11 Maintenance

Tray Home and Park Positions

222

Figure 65 Tray in Park position

Figure 66 Tray in Home position

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installing a Syringe

Installation, Operati Part 4, Maintenance

To install a syringe (Figure 67):

1 Unplug the injector cable, and if desired, mount the injector on a parking post, or lay the injector tower on a work bench.

2 Open the injector door.

3 Slide the syringe carriage to the top position.

4 Open the syringe latch by swinging it in a counterclockwise direction.

5 Lift the plunger carrier to the top position.

6 Carefully pass the syringe needle through the guide hole in the needle support foot.

Figure 67 Installing a syringe

Plunger carrier

Plunger screw

Flange guide

Syringe carrier

Syringe latch

Needle support foot

Slide

Plunger

Flange

Needle

Barrel

on, and Maintenance 223 and Troubleshooting

224

11 Maintenance

7 Align the syringe flange with the flange guide and press the syringe into place, keeping the needle end in the guide hole of the needle support foot. Make sure that the flat edge of the syringe flange faces out (Figure 68).

NOTE Failure to correctly install the syringe flange into the flange guide will result in damage to the syringe plunger.

8 Close the syringe latch by swinging it clockwise until it snaps in place.

9 Loosen the plunger screw entirely by turning it counterclockwise until the stop is reached.

10 Slide the plunger carrier down until it is completely over the syringe plunger, and tighten the plunger thumb screw until finger-tight.

11 Manually move the plunger carrier up and down. If the syringe plunger does not move along with the carrier, repeat the previous steps until installed correctly. Be sure the plunger thumb screw is secure and tight. If the carrier is not completely attached to the syringe plunger it may become detached after a few injections.

Figure 68 Syringe flange orientation

Flange

Flange guide

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

CAUTION Repeating this movement can damage the syringe.

12 Verify that the needle is inside the guide hole of the needle support foot. The needle should be straight and pass freely through the needle guide hole.

If the needle is bent or is outside the guide hole, remove the syringe and reinstall. See Figure 69 for a properly installed syringe.

13 Close the injector door.

Figure 69 Syringe carriage and needle support with syringe installed

Syringe latch (closed)

Needle support foot

on, and Maintenance 225 and Troubleshooting

226

11 Maintenance

14 Do the following only if the injector tower was removed from the mounting post during installation:

a If necessary, plug in the injector cable.

b Install the injector on the mounting post. See Installing the G4513A Injector on page 58 for details.

c If you have a sample tray, calibrate the ALS system. See Calibrating the ALS System on page 249 for details.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Removing a Syringe

Installation, Operati Part 4, Maintenance

To remove a syringe:

1 Unplug the injector cable, and if desired, mount the injector on a parking post.

2 Open the injector door.

3 Slide the syringe carriage to the top position.

4 Completely loosen the plunger thumb screw until it reaches the stop, and lift the plunger carrier off of the syringe plunger.

5 Open the syringe latch by swinging it in a counterclockwise direction.

CAUTION Be careful not to bend the syringe needle. Only pull the syringe out of the carriage until clear. The needle bends easily when still seated in the needle support guide.

6 Carefully pull the top of the syringe out of the flange guide, then lift the needle out of the needle support foot.

To install a syringe, see Installing a Syringe on page 223 for details.

on, and Maintenance 227 and Troubleshooting

11 Maintenance

Changing the Turret

228

Two turrets are provided with the G4513A injector (Figure 70). The 16-sample standalone turret, and the 3-sample transfer turret.

The labeled positions are defined in Table 15 and Table 16.

Figure 70 Injector turrets

Table 15 Standalone turret labels

Position Label Bottle/Vial

1 through 14 1 through 14 Sample vials

15 15 L2

Sample vial Layer 2 bottle

16 16 L3

Sample vial Layer 3 bottle

17 W Waste bottle

18 B Solvent B bottle

19 A Solvent A bottle

Transfer turret (3 samples)

Standalone turret (16 samples)

A B

16

3

1

5

7

9 12

14

Top views.

W

15

13

11 10 8

6

4

2 A

A2

A4 L3

L1

WB

WA

WA B2

A6

A3

A5

B

B3 B4 WA

WB

L2

L3

L2

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

To change your turret, use the following instructions for proper replacement.

1 Remove all vials from the turret.

2 Unplug the injector cable and place the injector tower on a parking post.

3 Open the injector door.

4 Remove the syringe. See Removing a Syringe on page 227.

5 While holding the turret in place with your hand, completely loosen the three T-10 Torx screws that fasten the turret cap to the motor hub.

6 Remove the turret cap.

7 Rotate the turret to the home position (with the turret injector opening facing the injector carriage).

8 Lift the turret off of the motor hub, then slide out. Avoid contact with the injector tower when removing the turret.

Table 16 Transfer turret labels

Position Label Bottle/Vial

1 L1 Dedicated vial transfer position A Layer 1 bottle

2 L2 Configurable vial transfer position B Layer 2 bottle

3 L3 Configurable vial transfer position C Layer 3 bottle

4 and 5 WB Waste B1 - B2 bottles

6 through 8 WA Waste A1 - A3 bottles

9 through 12 B - B4 Solvent B1 - B4 bottles

13 through 18 A - A6 Solvent A1 - A6 bottles

on, and Maintenance 229 and Troubleshooting

230

11 Maintenance

9 Rotate the motor hub so the tabs on the motor hub face outward.

Figure 71 Removing the turret

Turret

Turret cap

Turret cap arms

Turret injector opening

Motor hub tabs

Motor hub

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

10 Reinstall the turret. Align the inner groove on the turret with the motor hub tabs, then slide the turret onto the motor hub. The turret should sit flat on the hub.

11 Reinstall the turret cap. Align the turret cap arms with the turret injector opening and place the turret cap in place.

12 Tighten the three T-10 Torx screws.

13 Install the syringe. See Installing a Syringe for more information.

14 Close the injector door.

15 Remove the injector tower from the parking post and install the injector on the mounting post. See Installing the G4513A Injector for details.

16 Plug in the injector cable.

17 When powered on, the injector verifies the turret type. A fault light will show if the turret is not installed correctly.

18 Align the injector. See Aligning the Injector on page 245 for details.

19 If you installed a transfer turret and are using a sample tray, then you must calibrate your ALS system. See Calibrating the ALS System on page 249 for details.

Turret cap arms

Turret injector opening

on, and Maintenance 231 and Troubleshooting

11 Maintenance

Adapting for Cool On-Column Injection

232

The 7693A injectors can inject samples directly into 250-m, 320-m, and 530-m columns in GCs with a cool-on-column inlet.

When performing cool on-column injections, the injector:

Slows the carriage speed so the overall injection time increases to 500 milliseconds.

Lowers the tip of the syringe needle an additional 19 mm into the column.

To adapt the injector and GC for cool on-column use, follow these steps:

1 If necessary, remove the current syringe from the injector. See Removing a Syringe on page 227 for more information.

2 If necessary, remove the injector from the mounting post and unplug the injector cable. If desired, park it on a parking post.

3 Select the on-column syringe needed for the column size. See your Agilent catalog for consumables and supplies, your GC operating documentation, and the Agilent web site (www.agilent.com/chem) for a list of parts.

4 Replace the needle support foot with the supplied on-column foot. See Replacing the Needle Support Foot on page 233 for more information.

5 Install the on-column syringe. See Installing a Syringe on page 223 for more information.

6 Prepare the GC inlet. See your GC operating documentation for instructions.

Check your needle to column size.

Verify that the insert matches the needle size.

If necessary, replace the septum.

For a list of other consumables needed to perform these injections, see your GC operating documentation.

7 Plug in the injector cable.

8 Install the injector on the mounting post. See Installing the G4513A Injector for details.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Replacing the Needle Support Foot

Installation, Operati Part 4, Maintenance

You must use either the standard needle support foot (Figure 72) or the on-column needle support foot depending on the type of injection.

Replace the needle support foot when you change injection types or when the support foot shows signs of wear:

1 Open the injector door.

2 Remove the syringe. See Removing a Syringe on page 227 for details.

3 Slide the syringe carriage up to the top position.

4 Completely remove the T-10 Torx screw from the support foot. Be careful to not let the screw fall into the turret assembly.

5 Slide off the support foot.

6 Slide on the new support foot.

7 Replace the T-10 Torx screw and tighten.

Figure 72 Replacing the needle support foot

Standard needle guide

On-column needle guide

on, and Maintenance 233 and Troubleshooting

234

11 Maintenance

8 Install the appropriate syringe. See Installing a Syringe on page 223 for details.

9 Close the injector door.

10 Align the injector. See Aligning the Injector on page 245 for details.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Adapting for Syringes Over 100 L

Installation, Operati Part 4, Maintenance

The injectors can perform enhanced sample handling injections using syringes over 100 L. To adapt the injector and GC for enhanced sample handling injections, follow these steps:

1 If necessary, remove the current syringe from the injector. See Removing a Syringe on page 227 for details.

2 Replace the standard syringe carriage assembly with the G4521A Enhanced Sample Handling Syringe Carriage assembly. See Replacing the Syringe Carriage Assembly on page 236 for details.

3 Install the syringe needle support foot supplied with the G4521A Large Volume Syringe Carrier. See Replacing the Needle Support Foot on page 233 for details.

4 Install the appropriate syringe. See Installing a Syringe on page 223 for details.

5 If necessary, reinstall the injector on the GC. See Installing the G4513A Injector for details.

6 Align the injector. See Aligning the Injector on page 245 for details.

7 If you have a sample tray, calibrate the ALS system. See Calibrating the ALS System on page 249 for details.

on, and Maintenance 235 and Troubleshooting

11 Maintenance

Replacing the Syringe Carriage Assembly

236

Use the standard syringe carriage assembly (G4513-67570) (Figure 73) for injections up to 100 L, and the G4521A Enhanced Sample Handling Syringe Carriage (G4521-63000) (Figure 74) for injections over 100 L.

Figure 73 Standard syringe carriage assembly (G4513-67570)

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

To replace the syringe carriage assembly:

1 Remove all vials and bottles from the turret, and disconnect the injector cable from the GC.

2 If desired, remove the injector from the mounting post and mount the injector tower on a parking post.

3 Open the injector door.

4 Remove the syringe. See Removing a Syringe on page 227 for details.

5 Remove the turret. See Changing the Turret on page 228 for details.

6 Slide the syringe carriage assembly down until the assembly cable is accessible below the tower casing, and detach the cable from the assembly.

Figure 74 Enhanced Sample Handling syringe carriage assembly (G4521-63000)

on, and Maintenance 237 and Troubleshooting

238

11 Maintenance

7 Completely loosen and remove the T-10 Torx screw at the top of the syringe carriage assembly.

T-10 Torx screw

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

8 Slide the syringe carriage assembly completely up until the flange and latch are accessible.

Figure 75 Removing the syringe carriage assembly

Plunger carrier

Syringe carriage

Cable

T-10 Torx screw

Flange

Latch

on, and Maintenance 239 and Troubleshooting

240

11 Maintenance

9 Completely loosen and remove the T-10 Torx screw above the syringe latch.

10 Carefully remove the syringe carriage assembly from the injector carriage.

11 Slowly position the replacement syringe carriage assembly on the injector carriage. The syringe carriage assembly will hang in place when positioned correctly.

12 Completely tighten the T-10 Torx screw above the syringe latch.

T-10 Torx screw

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

13 Slide the syringe carriage assembly completely down until the cable input jack on the assembly is accessible below the injector casing.

Cable input jack

on, and Maintenance 241 and Troubleshooting

242

11 Maintenance

14 Completely tighten the T-10 Torx screw at the top of the syringe carriage assembly.

15 Connect the assembly cable to the syringe carriage assembly.

16 Slide the syringe carriage assembly completely up.

17 Make sure you have the appropriate needle support foot installed. See Replacing the Needle Support Foot on page 233 for details.

18 Install the turret. See Changing the Turret on page 228 for details.

19 Install the syringe. See Installing a Syringe on page 223 for details.

20 Close the injector door.

21 If necessary, plug in the injector cable and install the injector tower on the mounting post. See Installing the G4513A Injector for details.

22 Align the injector. See Aligning the Injector on page 245 for details.

23 If you have a sample tray, calibrate the ALS system. See Calibrating the ALS System on page 249 for details.

T-10 Torx screw

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Replacing a Syringe Needle

Installation, Operati Part 4, Maintenance

The stainless steel needles used for 250-m and 320-m injections must be inserted into a glass syringe barrel. Select the correct size needle for the column you will use.

Needles for 250-m injections have silver-colored stops. Needles for 320-m injections have gold-colored stops. See your Agilent consumables and supplies catalog or the Agilent web site (www.agilent.com/chem) for a list of syringes and needles.

Table 17 On-column autosampler syringes

Volume (L) Description Unit Part No.

5 Removable needle, barrel only 5182-0836

Stainless steel needle for 530 m column 3/pk 5182-0832

Stainless steel needle for 320 m column 3/pk 5182-0831

Stainless steel needle for 250 m column 3/pk 5182-0833

Plunger button 10/pk 5181-8866

on, and Maintenance 243 and Troubleshooting

244

11 Maintenance

To insert a needle into a syringe barrel (Figure 76):

1 Unscrew the syringe barrel cap and remove the spring.

2 Make sure the needle has a PTFE disk (Figure 76). If the syringe barrel does not have the PTFE disk, use the instructions in the syringe box to wrap the needle yourself.

3 Slide the spring and the cap down over the needle.

4 Insert the needle into the syringe barrel.

5 Screw the cap back on the syringe barrel.

Figure 76 Syringe parts

Plunger

Barrel

PTFE disk

Needle

Spring

Cap

PTFE disk

Stop

Needle

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Aligning the Injector

Installation, Operati Part 4, Maintenance

This section explains how to perform the injector alignment procedure. The injector has been factory-aligned prior to shipment. The alignment procedure should only be run if the injector hardware configuration has changed or if the Align Mode light comes on.

NOTE Agilent does not recommend performing this procedure unless the Align Mode light turns on. It is not necessary to align the turret after changing turrets.

If the top, orange Align Mode light is on, the injector will not operate again until the alignment procedure has successfully completed.

To align the injector:

1 Remove all vials from the turret.

2 Open the injector door and remove the syringe. See Removing a Syringe on page 227 for details.

3 Close the injector door.

4 Use a long, narrow object to press the recessed Alignment button above the indicator lights. Avoid contact with anything behind or around the button inside the recessed hole (Figure 77).

Figure 77 Aligning the injector

Alignment button

Align Mode

Fault

Ready

on, and Maintenance 245 and Troubleshooting

246

11 Maintenance

The orange Align mode light is on during the alignment procedure:

a The turret rotates to verify that the syringe was removed, then rotates to determine what type of turret is installed.

b The syringe carriage moves all the way down, homes, then moves back up again.

c The syringe carriage steps down until it touches the turret. This sets its position relative to the turret.

d The plunger moves to calibrate stops.

e The syringe carriage moves down and the turret rotates to set its position relative to the syringe carriage position.

NOTE If any of these steps fail, the process will halt and the fault light will shine. If it halts at step b, confirm that the turret is installed correctly and press the Alignment button again. If it fails again, unplug the injector, reconnect it, and try one more time before obtaining Agilent service.

5 When the injector stops and the green Ready light is on, the calibration is finished.

If this alignment was user-initiated (orange Align Mode light was off before pressing the Alignment button) and alignment fails, unplug the injector, reconnect it, and it will reset to the old alignment values.

6 Open the injector door and install the syringe. See Installing a Syringe on page 223 for details.

7 Close the injector door.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Aligning the Sample Tray

Installation, Operati Part 4, Maintenance

This section explains how to perform the sample tray alignment procedure. The sample tray has been factory-aligned prior to shipment. The alignment procedure should only be run if the Align Mode light comes on.

NOTE Agilent does not recommend performing this procedure unless the Align Mode light turns on or if Agilent support requests it to be performed.

If the left, orange Align Mode light is on, the sample tray will not operate again until the alignment procedure has successfully completed.

To align the sample tray:

1 Use a long, narrow object (such as a pen tip) to press the recessed Alignment button to the left of the indicator lights. Avoid contact with anything behind or around the button inside the recessed hole (Figure 78).

The orange Align mode light blinks during the following steps:

a The gripper power calibrates.

b The gantry calibrates the X, Y, and Z home and span settings.

c The gantry is zeroed to calibration position (X, Y, Z).

Figure 78 Align the sample tray

Alignment button

Align Mode

Fault

Ready

NOTE If any of these steps fail, the process will halt. Press the Alignment button again. If it fails again, unplug the sample tray, reconnect it, and try one more time before obtaining Agilent service.

on, and Maintenance 247 and Troubleshooting

248

11 Maintenance

2 When the sample tray stops in the Home position and the green Ready light is on, the calibration is finished.

If this alignment was user-initiated (orange Align Mode light was off before pressing the Alignment button) and alignment fails, unplug the sample tray, reconnect it, and it will reset to the old alignment values.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Calibrating the ALS System

Installation, Operati Part 4, Maintenance

The ALS system calibration process aligns the sample tray with the injector turret position so vial transfers occur without incident. Calibration should be performed if a calibration does not exist, and also as a routine maintenance procedure.

Calibrating the ALS system is recommended if any ALS components are moved.

To calibrate your ALS system:

1 Place the calibration vial (G4514-40588) in tray position 1 (Figure 79).

Figure 79 Tray position 1

on, and Maintenance 249 and Troubleshooting

250

11 Maintenance

2 Remove any vials from transfer turret positions L1, L2, and L3 for all installed injectors (Figure 80).

3 Start the ALS system calibration:

On a 7890A GC, use the GC front keypad and select [Options] Calibration > ALS > Start Calibration.

On a 6890 Series GC, use the GC front keypad and select [Options] Calibration > Sample tray > Start Calibration.

On an Intuvo 9000 GC:

Figure 80 Transfer turret positions L1, L2, L3 (top view)

L1

L2

L3

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

The following calibration process will occur for all installed injectors:

a The tray assesses the turret alignment by placing the calibration vial into turret position L1 and returning to sample tray position 1.

b The tray tests the vial height and turret position by using the alignment tab between positions L1 and L2 on the transfer turret.

c The tray verifies the turret alignment by placing the calibration vial into turret position L1 and returning it to sample tray position 1.

4 When the calibration process is complete, the green Ready status light will come on and the gantry will stop in the Home position (Figure 66 on page 222).

Remember to replace any vials that were moved for the calibration process.

on, and Maintenance 251 and Troubleshooting

11 Maintenance

Replacing Power Fuses in the G4517A ALS Controller

252

The G4517A ALS Controller uses two replaceable fuses to protect its electronics from line voltage problems. If blown, replace them as described below.

CAUTION Fuses blow for a reason. The most likely cause at first installation is an incorrect power configuration (see Verify the power configuration on page 100). Otherwise, normal operation should not blow the fuses. If the fuses blow frequently, identify and correct the problem

1 Turn off the controller.

2 Remove the power cord.

3 Remove the fuse holder module using a small, flat-bladed screwdriver. (See Installing the G4526A/G4517A ALS Controller (6890A GCs) for details.)

4 Pull the tab out using a small, flat-bladed screwdriver.

5 Remove the fuses.

6 Install the new fuses (Figure 81).

7 Push the tabs closed.

Figure 81 Proper fuse orientation

250V

TYPE T

2A 2 A

2 A 250 VAC

2 A 250 VAC

Tabs

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Maintenance 11

Installation, Operati Part 4, Maintenance

8 Reinstall the fuse holder module, making sure the orientation is correct for the line voltage input. See Installing the G4526A/G4517A ALS Controller (6890A GCs) for details.

on, and Maintenance 253 and Troubleshooting

254

11 Maintenance

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

12 Faults and Errors

Faults 256

Injector faults 256

Sample tray faults 258

Error Messages 260

Sometimes things do not work as expected. In most cases, the nature of the problem is signalled either by status lights on the injector and sample tray or by error messages on the GC display. If this happens, use this chapter to discover the probable cause and the corrective action to take.

255Agilent Technologies

12 Faults and Errors

Faults

Injector faults

256

The injector status is indicated by the three lights on the front injector panel (Figure 82).

During normal operation, the green Ready light is on. If the injector is busy, the green Ready light is flashing.

If another combination of lights is on, an error has occurred.

Use the information in this chapter to try to solve or identify the problem before obtaining Agilent service.

Figure 82 G4513A Injector status lights

Table 18 Injector status lights

Lights Possible cause Action

All lights are off. The line voltage to the GC is off. The injector cable or

connection to the GC is bad. Your GC requires service.

1 Verify the injector is properly connected to the GC.

2 Check the power source for your GC.

3 Obtain Agilent service.

Align Mode light

Fault light Ready light

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Faults and Errors 12

Installation, Operati Part 4, Maintenance

Fault light is on. The injector door is open. Injector is not properly

mounted to the GC.

1 Ensure that the injector door is closed.

2 If the fault light stays on, obtain Agilent service.

3 Ensure that the injector is mounted properly. For more information, see Installing the G4513A Injector on page 58.

4 Ensure the correct mounting post is installed. See Installing the G4513A Injector on page 58.

5 If the Fault light stays on, obtain Agilent service.

Fault light is flashing two times.

Syringe error. 1 Re-install or replace the syringe.

Fault light is flashing three times.

Turret error. 1 Re-install the turret.

Fault light is flashing four times.

Plunger error.

Align Mode light is on. The system was not initialized. There is an injector memory

error.

1 Verify the turret is properly installed. See Changing the Turret on page 228.

2 Perform the alignment procedure to initialize the system. See Aligning the Injector on page 245.

3 If alignment fails, obtain Agilent service.

Align Mode light is flashing.

Customer pushed the align mode button.

1 The alignment and calibration process is currently underway. Let the process finish.

All lights are on. There is a board failure. There is a firmware revision

conflict.

1 Check all cable connections. 2 Turn the instrument off, then on

again. 3 If the lights remain on, obtain

Agilent service.

Table 18 Injector status lights (continued)

Lights Possible cause Action

on, and Maintenance 257 and Troubleshooting

258

12 Faults and Errors

All lights are flashing. Wrong driver board installed. 1 Install the correct driver board. 2 Check firmware revision. 3 If lights remain flashing, obtain

Agilent service.

Table 18 Injector status lights (continued)

Lights Possible cause Action

Sample tray faults

The sample tray status is indicated by the three lights on the front panel (Figure 83).

During normal operation, the green Ready light is on. If the sample tray is busy, the green Ready light is flashing.

If another combination of lights is on, an error has occurred.

Use the information in this chapter to try to solve the problem before obtaining Agilent service.

Figure 83 G4514A Sample Tray status lights

Align Mode light

Fault light

Ready light

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Faults and Errors 12

Installation, Operati Part 4, Maintenance

Table 19 Tray status lights

Lights Possible cause Action

All lights are off. The device is unplugged.

Fault light is on. System fault.

Fault light is flashing one time.

A vial is lost or missing.

Fault light is flashing two times.

X-axis error.

Fault light is flashing three times.

Y-axis error.

Fault light is flashing four times.

Z-axis error.

Fault light is flashing five times.

Gripper error.

Align Mode light is on. The system was not initialized. There is a tray memory error.

1 Perform the alignment procedure to initialize the system. See Aligning the Injector on page 245.

2 If alignment fails, obtain Agilent service.

Align Mode light is flashing.

Customer pushed the align mode button.

1 The alignment and calibration process is currently underway. Let the process finish.

All lights are on. There is a board failure. There is a firmware revision

conflict.

1 Check all cable connections. 2 Turn the instrument off, then on

again. 3 If the lights remain on, obtain

Agilent service.

All lights are flashing. Wrong driver board installed. 1 Install the correct driver board. 2 Check firmware revision. 3 If lights remain flashing, obtain

Agilent service.

on, and Maintenance 259 and Troubleshooting

12 Faults and Errors

Error Messages

260

Table 20 lists the sampler error messages as reported on the GCs. Error messages are not displayed on 6890A or 6890 Plus GCs. If you receive an error message that is not shown below, record it. Make sure that your GC is properly configured and that your sample vials and equipment match your method and/or sequence. If the problem continues, report your error message to Agilent service.

Table 20 Error messages

Message Probable cause Suggested action

Bottle in gripper The sample vial was not delivered properly and stayed in the tray gripper.

1 Remove the vial and return it to its position in the tray.

2 Ensure that the vial racks are snapped into place.

3 Ensure that the injector is plugged into the correct connector on the back of the GC. For 6890, ensure it is configured properly. See Connecting the Cables on page 73.

4 Check the deliver to location for the vial and verify that the location is empty and free from obstructions.

5 Make sure that the injector is upright/vertical on the GC.

6 Restart the sequence. 7 If the error occurs again, obtain

Agilent service.

Front (or Back) door open or Injector not mounted

See Faults on page 256.

Front (or Back) injector com error There is a communications error between the injector and the GC.

Obtain Agilent service.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Faults and Errors 12

Front (or Back) injector incomplete injection

The syringe needle is bent. The plunger or syringe carriage is

operating incorrectly during injection.

1 See Correcting Syringe Problems on page 273.

2 Remove the syringe from the injector and check the plunger for stickiness or binding. Replace the syringe if necessary.

3 Restart the sequence. 4 If the error occurs again, obtain

Agilent service.

Front (or Back) injector reset There is an interruption in the power supply from the GC.

Obtain Agilent service.

Front (or Back) plunger error The syringe plunger is sticking or not securely connected to the plunger carrier.

The plunger solenoid is binding. The plunger carrier encoder is

inoperable.

1 Remove the syringe and check it for plunger stickiness or binding. Replace the syringe if necessary. For more information, see Inspecting a Syringe on page 177.

2 Check the viscosity of the sample against the viscosity parameter. Reset the viscosity parameter if necessary.

3 Restart the sequence. 4 If the error occurs again, obtain

Agilent service.

Front (or Back) syringe error The syringe carriage motor is defective.

The syringe is not currently installed or is an incorrect type.

The syringe carriage sensor is inoperable.

1 Ensure the syringe is installed correctly. For more information, see Installing a Syringe on page 178.

2 Ensure the syringe meets specifications.

3 If the syringe needle is bent, see Correcting Syringe Problems on page 273.

4 Restart the sequence. 5 If the error occurs again, obtain

Agilent service.

Table 20 Error messages (continued)

Message Probable cause Suggested action

Installation, Operation, and Maintenance 261 Part 4, Maintenance and Troubleshooting

12 Faults and Errors

Front (or Back) turret error Something has interfered with the turret rotation.

The turret motor/encoder assembly is inoperable.

The turret type was changed while the power was on and the turret alignment procedure was not performed.

Turret is loose.

1 Clear any obstructions. 2 Check the Align Mode light. If it is

on, perform the alignment procedure. See Aligning the Injector on page 245.

3 Tighten the top of the turret. 4 If the error occurs again, obtain

Agilent service.

Injector not present There is a board failure in the injector or GC.

The injector cable is bad or not securely connected to the GC.

There is a cable failure in the GC. Your method specifies an incorrect

injector location (method mismatch).

1 Make sure that the injector-to-GC cable connection is secure.

2 Check your method to make sure it uses the appropriate injector location.

3 If the error remains, obtain Agilent service.

Injector offline There is a board failure in the injector or GC.

The injector cable is bad or not connected.

There is a cable failure in the GC.

1 Make sure that the injector to GC cable connection is secure.

2 If the error remains, obtain Agilent service.

No Bar Code Reader Bar Code Reader cable not securely connected.

Bar Code Reader is defective. Tray is defective.

1 Ensure the Bar Code Reader cable connection is secure.

2 If the problem continues, obtain Agilent service.

No bottle in gripper The sample vial was not found by the gripper.

The gripper could not grasp the vial. The vial was dropped during transfer

to or from the turret. The sensor in the gripper is

defective. The vial does not meet

specifications.

1 Make sure that the sample vials are in the locations specified by the sequence.

2 Make sure that the sample vials meet recommended specification.

3 If you are using adhesive labels, verify that the labels are properly installed. See Label a sample vial on page 188.

4 If the error recurs frequently, obtain Agilent service.

Table 20 Error messages (continued)

Message Probable cause Suggested action

262 Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Faults and Errors 12

Tray not present There is a board failure in the tray or GC.

The tray cable is bad or not connected between the GC and tray.

There is a cable failure in the GC.

1 Make sure that the tray cable connection is secure.

2 Replace the tray cable. 3 If the error remains, obtain Agilent

service.

Tray offline There is a board failure in the tray or GC.

The tray cable is bad or not connected.

There is a cable failure in the GC.

1 Make sure that the tray to GC cable connection is secure.

2 Replace the tray cable. 3 If the error remains, obtain Agilent

service.

Invalid sequence The sequence is set up for the wrong injection device.

Hardware required by the sequence is not installed and configured.

The GC configuration was changed during sequence execution.

The injector cable is bad or not connected properly.

1 Make sure that the connection to the GC is secure.

2 Verify the sequence parameters against the GC configuration.

3 If the error remains, obtain Agilent service.

No injector The cabling connection to the GC became loose during a run.

An injector board or GC board failed during a run.

1 Make sure that the connection to the GC is secure.

2 If the error remains, obtain Agilent service.

Prerun >10 min The GC is Not Ready. Check for Not Ready and other GC messages to determine the cause.

Sampler Offline (6890A/Plus GCs Only)

You are attempting to upload or download an ALS workfile to an ALS system that is not powered on or connected.

Make sure that the connection to the GC is secure.

Table 20 Error messages (continued)

Message Probable cause Suggested action

Installation, Operation, and Maintenance 263 Part 4, Maintenance and Troubleshooting

12 Faults and Errors

264 Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

13 Troubleshooting

Symptom: Variability 266

Symptom: Contamination or Ghost Peaks 268

Symptom: Smaller or Larger Peaks Than Expected 269

Symptom: Sample Carryover 271

Symptom: No Signal/No Peaks 272

Correcting Syringe Problems 273

Correcting Sample Vial Delivery Problems 274

The information in this chapter will assist you in keeping the sampler running smoothly.

If the chromatogram is not satisfactory, clearly something is wrong. Use this chapter to determine the probable cause and, in many cases, the cure for the problem.

This chapter deals only with sampler-related problems. However, many of the symptoms described here could also come from other sources, particularly the stability of the GC temperature and its gas supplies.

If you cannot correct the problem, obtain Agilent service.

265Agilent Technologies

13 Troubleshooting

Symptom: Variability

266

Figure 84 Retention times or areas are not reproducible

Table 21 Variability problems

Possible cause Action

Inlet septum is leaking. If the septum is leaking, replace it. If the septum you replaced experienced less than 200 injections, check for the following possible problems to prevent premature septum failure:

The septum retainer nut is too tight. The syringe needle is not straight. The syringe is not installed correctly.

Syringe is worn or dirty. If the syringe looks dirty or the plunger is sticking, clean the syringe with an appropriate solvent or follow the syringe manufacturers cleaning instructions.

Sample volume is too low or too high.

Check sample level. If the sample vials are not filled correctly, evaporation or contamination may affect the analysis. The sample level hold will be approximately half the volume of the vial. See Fill a sample vial on page 189.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Troubleshooting 13

Installation, Operati Part 4, Maintenance

Vial caps are loose. Check vial caps. If you can turn the vial crimp cap by hand, they are too loose. Loose caps may cause volatile samples to change concentration over time. See Cap a sample vial on page 190. Screw caps, if not sufficiently tight, can come loose in the mixer and even come off.

Sample is not stable. Check the sample stability. Some samples change with heat or ultraviolet light. There are several ways to reduce changes to unstable samples:

Use the vial racks to cool the sample. Use amber sample vials. Store the samples in a protected environment.

Sample size varies. Install a new syringe. If the sample size varies, the syringe is probably not precise or the plunger is worn. Variation may be due to syringes with removable needles because of dead volume or needle-to-needle variation.

Air bubbles are in the needle. If air bubbles are in the needle, user variable speed and a sample draw speed slow enough to avoid bubble formation. See Setting the Injector Parameters on page 162. If this does not help and the sample is viscous, try the following:

Increase the viscosity delay time. Use the vial racks to warm the sample. Dilute the sample in an appropriate low-viscosity

solvent.

Table 21 Variability problems (continued)

Possible cause Action

on, and Maintenance 267 and Troubleshooting

13 Troubleshooting

Symptom: Contamination or Ghost Peaks

268

Table 22 Contamination or ghost peak problems

Possible cause Action

Vial cap septum is dissolving in solvent. Ghost peaks sometimes appear when small pieces of septum material dissolve in the sample. Make several blank runs to determine the presence or absence of the ghost peaks.

Check for the following: Be sure the vial septum is flat. If the vial septum is not

flat, the needle tends to core the septum and drop pieces into the sample. See Cap a sample vial on page 190.

Check the needle. If the syringe needle has burrs, it could cut pieces of the septum and push them into the sample.

Check the vial septum. If the vial septum is not resistant enough to the solvent you are using, try a more resistant type.

Sample vials are contaminated. Ghost peaks are sometimes caused by contaminated sample vials. Try new or clean vials to see if ghost peaks disappear. Store new vials in a contaminant-free location.

Inlet septum is giving off volatiles. Make several blank runs with a small piece of aluminum foil backing the inlet septum. If the contamination peaks disappear, they were probably due to the septum. Try replacing the septum you usually use with another type.

Column is contaminated. High molecular weight samples that contain residues may cause the syringe, the inlet liner, or the first few inches of column to become contaminated.

Do the following: Replace or clean and deactivate the inlet liner. Examine the first few inches of a capillary column for

foreign material by holding a light behind it. If possible, remove the contaminated section.

Sample is not stable. Some samples change with heat or ultraviolet light. Check the sample stability. There are several ways to reduce the change:

Use the tray quadrant to cool the sample. Use amber sample vials. Store the samples in a protected environment.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Troubleshooting 13

Symptom: Smaller or Larger Peaks Than Expected

Installation, Operati Part 4, Maintenance

Figure 85 Smaller or larger peaks than expected

Table 23 Peak size problems

Possible cause Action

You are comparing a chromatogram without needle fractionation against one with needle fractionation.

Check your injection mode. In the normal injection mode, the sampler uses fast injection to deliver a representative amount of the sample. Fast injection minimize needle fractionation. Chromatograms from manual injection or slower auto injection devices show higher levels of low molecular weight materials versus higher molecular weight materials because the volatiles boil out of the needle faster than the higher weight materials.

Automated injection

Manual injection

on, and Maintenance 269 and Troubleshooting

270

13 Troubleshooting

You are using a packed inlet and a 530-m column.

Check your inlet. Capillary columns used with packed inlets have some inherent sample discrimination characteristics.

There is a leak in the GC system. Replace the septum and check the fitting for leaks. If the leaking septum has experienced less than 200 injections. To prevent future premature failures, be sure that:

The septum retainer nut is not too tight. The syringe needle is straight. The syringe is installed correctly.

Sample is not stable. Some samples change with heat or ultraviolet light. Check the sample stability. There are several ways to reduce the change:

Use the vial racks to cool the sample. Use amber sample vials. Store the samples in a protected environment.

Vial caps are loose. Check the vial caps. Loose vial caps can cause selective loss of lighter materials from a sample. The cap should not rotate easily if installed properly. See Cap a sample vial on page 190.

Table 23 Peak size problems (continued)

Possible cause Action

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Troubleshooting 13

Symptom: Sample Carryover

Installation, Operati Part 4, Maintenance

Figure 86 Blank run showing carryover peaks

Table 24 Carryover problems

Possible cause Action

Number or type of washes is insufficient.

Check the run parameters for the number of sample and solvent washes. The number of washes needed depends on your application. See Sample Carryover on page 142.

You ran out of solvent. Check the solvent bottles. If the solvent level is below 2.5 mL, the syringe cannot reach the solvent. Replace the remaining solvent with 4 to 4.5 mL of fresh solvent. See Preparing Solvent and Waste Bottles on page 192. Check the waste bottles. If the waste level is near the neck of the bottle, replace it with an empty bottle.

Syringe is worn or dirty. If the syringe look dirty or the plunger is sticking, clean the syringe with an appropriate solvent or follow the syringe manufacturers cleaning instructions. If the syringe seems worn, replace it.

Samples (vial-to-vial) are of immiscible types.

In this situation, the sample and solvent washes may not rinse the syringe properly. Increase the number of wash cycles or use a solvent that rinses a variety of sample types.

on, and Maintenance 271 and Troubleshooting

13 Troubleshooting

Symptom: No Signal/No Peaks

272

Table 25 Signal/Peak problems

Possible cause Action

Syringe plunger is malfunctioning. Verify that the syringe plunger is secured by the plunger screw. If the plunger screw is loose, tighten it. SeeInstalling a Syringe on page 178. Check the syringe needle for plugging. If the syringe is plugged, replace or clean the syringe.

Sample level is too low in vial. If there is no or very little sample in the vial, the needle may not be able to reach it. See Fill a sample vial on page 189. Alternately, you may edit your method to adjust the needle sampling depth. See Sampling offset in Setting the Injector Parameters on page 162.

Sample is viscous. If the sample is viscous, try the following: Increase the viscosity delay time. Use the vial racks to warm the sample. Dilute the sample in an appropriate low-viscosity

solvent.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Troubleshooting 13

Correcting Syringe Problems

Installation, Operati Part 4, Maintenance

WARNING When troubleshooting the injector, keep your hands away from the syringe needle. The needle is sharp and may contain hazardous chemicals.

Several things can cause syringe needles to bend. When you find one, check for the following conditions before installing a replacement:

Was the syringe installed properly in the syringe carriage?

Are you using the correct syringe type? Is the combined length of the syringe barrel and needle 126.5 mm? For more information, see Selecting a Syringe on page 174.

Is the needle support foot clean? Remove any residue or septum deposits. For more information, see Periodic Maintenance on page 220.

If performing a cool on-column injection, is the correct insert for your syringe installed in the cool on-column inlet? For more information, see Adapting for Cool On-Column Injection on page 232.

Is the GC septum nut too tight? For more information, see your GC operating documentation.

Is the septum of the crimp cap centered over the sample vial? For more information, see Cap a sample vial on page 190.

Are the inside diameters of the sample vial, microvial insert, and vial cap septum at least 5 mm? For more information, see Preparing a Sample Vial on page 186.

on, and Maintenance 273 and Troubleshooting

13 Troubleshooting

Correcting Sample Vial Delivery Problems

274

When you find a mishandled sample vial, check:

Are the vial caps installed correctly?

Are there folds or wrinkles in the crimp cap, especially near the neck of the sample vial? For more information, see Preparing a Sample Vial on page 186.

If you use labels on the sample vials, are they the correct size? For more information, see Label a sample vial on page 188.

If you use labels on the sample vials, are they interfering with the gripper? For more information, see Label a sample vial on page 188.

Are there any obstacles to the sample tray arm or injector turret motions? Remove any obstacles.

Are the vial racks and turret in good condition? Clean any residue from the sample positions.

Does the bottom of a sample vial touch the top of the turret? To calibrate the ALS system, see Calibrating the ALS System on page 249.

Does the side of a sample vial touch the side of the turret hole as the vial is raised or lowered? To calibrate the ALS system, see Calibrating the ALS System on page 249.

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Agilent 7693A Automatic Liquid Sampler Installation, Operation and Maintenance

14 Replacement Parts

G4513A Injector 276

G4514A Sample Tray 278

G4517A ALS Controller (6890A GC Only) 280

G4516A ALS Interface Board (6890 Plus GC Only) 282

The following pages list the replacement parts for the 7693A ALS system. Also refer to the Agilent web site at www.agilent.com/chem for the most up-to-date replacement part and firmware listings.

275Agilent Technologies

14 Replacement Parts

G4513A Injector

276

Table 26 and Figure 87 list and show the replacement parts for the G4513A Injector module.

Table 26 G4513A Injector replacement parts

Item Description Part no. Qty/Assy

1 Transfer turret G4513-67730 1

2 Standalone turret G4513-40532 1

3 Needle support insert - Standard G4513-40525 1

4 Needle support insert - COC G4513-40529 1

5 Dual parking post 05890-61525 1

6 Injector mounting post G4513-20561 1

7 Injector module (new/exchange) G4513A 1

8 Communication cable G4514-60610 1

NS Packaging kit for G4513A Injector G4513-60810 1

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Replacement Parts 14

Figure 87 G4513A Injector replacement parts

1 2 3 4 5 6

7 8

Installation, Operation, and Maintenance 277 Part 4, Maintenance and Troubleshooting

14 Replacement Parts

G4514A Sample Tray

278

Table 27 and Figure 88 list and show the replacement parts for the G4514A Sample Tray.

Table 27 G4514A Sample Tray replacement parts

Item Description Part no. Qty/Assy

1 Vial rack, 3 per pack G4514-67505 1

2 L Key Torx T-20 8710-2430 1

3 5MM Hex L Key G4514-80524 1

4 Tray bracket insulating washer G4514-20529 1

5 M4 33.3 screw 0515-0437 1

6 M6 25 screw 0515-0192 3

7 Gripper finger caps G4514-60710 16

8 M4 0.7 screw 1390-1024 4

9 Calibration vial G4514-40588 1

10 Mounting bracket G4514- 63000 1

11 Bar code reader/mixer/heater G4515-64000 1

12 ALS main cable assembly G4514-60610 1

13 Vial rack label kit G4525-60701 3

NS Heating and cooling plate G4522A 1

NS Vial rack set of 3, with 12 label tags (4 colors)

G4525A 1

NS Packaging kit for G4514A Tray G4514-60810 1

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Replacement Parts 14

Figure 88 G4514A Sample Tray replacement parts

1

2

3

6

7

4 5

8 9

10

11

12

13

Installation, Operation, and Maintenance 279 Part 4, Maintenance and Troubleshooting

14 Replacement Parts

G4517A ALS Controller (6890A GC Only)

280

Table 28 and Figure 89 list and show the replacement parts for the G4517A ALS Controller (for 6890A GCs only).

WARNING Replacement of internal fuses and the battery can expose the servicing person to hazardous voltages and also exposes the controller to damage. Replacement is not covered in this manual. Refer servicing to qualified, Agilent-trained personnel.

Table 28 G4517A ALS Controller replacement parts

Item Description Part No. Qty/Assy

1 G4517A Controller (new) G4517-64000

2 Fuse, 2 A 250 VAC

NS Fuse, power supply PCA

NS Fuse, ALS controller PCA

NS Battery, 3 V .5 A, Lithium ion

Installation, Operation, and Maintenance Part 4, Maintenance and Troubleshooting

Replacement Parts 14

Figure 89 Replacement parts for the G4517A ALS Controller

250V

TYPE T

2A 2 A

USE ONLY WITH 250V

FUSES/EMPLOYER

UNIQUEMENT AVEC

DES FUSIBLES DE 250V

110-120 V

220-240 V

Back panel

2

1

2

Installation, Operation, and Maintenance 281 Part 4, Maintenance and Troubleshooting

14 Replacement Parts

G4516A ALS Interface Board (6890 Plus GC Only)

282

Table 29 G4516A ALS Interface Board replacement parts

Item Description Part no. Qty/Assy

NS ALS Interface Board controller G4516-64000 1

Manualsnet FAQs

If you want to find out how the 7693A Agilent works, you can view and download the Agilent 7693A Liquid Sampler Maintenance Manual V2 on the Manualsnet website.

Yes, we have the Maintenance Manual for Agilent 7693A as well as other Agilent manuals. All you need to do is to use our search bar and find the user manual that you are looking for.

The Maintenance Manual should include all the details that are needed to use a Agilent 7693A. Full manuals and user guide PDFs can be downloaded from Manualsnet.com.

The best way to navigate the Agilent 7693A Liquid Sampler Maintenance Manual V2 is by checking the Table of Contents at the top of the page where available. This allows you to navigate a manual by jumping to the section you are looking for.

This Agilent 7693A Liquid Sampler Maintenance Manual V2 consists of sections like Table of Contents, to name a few. For easier navigation, use the Table of Contents in the upper left corner.

You can download Agilent 7693A Liquid Sampler Maintenance Manual V2 free of charge simply by clicking the “download” button in the upper right corner of any manuals page. This feature allows you to download any manual in a couple of seconds and is generally in PDF format. You can also save a manual for later by adding it to your saved documents in the user profile.

To be able to print Agilent 7693A Liquid Sampler Maintenance Manual V2, simply download the document to your computer. Once downloaded, open the PDF file and print the Agilent 7693A Liquid Sampler Maintenance Manual V2 as you would any other document. This can usually be achieved by clicking on “File” and then “Print” from the menu bar.