Contents

Agilent Brave Metabolomics Workbench Handler User Guide PDF

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Summary of Content for Agilent Brave Metabolomics Workbench Handler User Guide PDF

This guide contains the following topics: About this guide on page 2 Utility description on page 3 Before you start on page 3 Accessing the utility on page 4 Running the protocol on page 5 Automation movements during the protocol on page 10

Bravo Metabolomics Workbench

Tip Transfer Utility User Guide For Research Use Only. Not for use in diagnostic procedures.

Bravo Metabolomics Workbench About this guide

2 Tip Transfer Utility Guide

About this guide

Overview

This guide describes the Tip Transfer utility for Bravo Metabolomics Sample Prep Platform. For more details on the Bravo Metabolomics Sample Prep Platform, see the Getting Started Guide in the Literature Library of the Bravo Metabolomics Workbench. The procedures in this guide assume that you are trained in the safe operation of the Bravo Platform.

WARNING Using controls, making adjustments, or performing procedures other than those specified in the user documentation can expose you to moving-parts hazards and hazardous voltage. Before using the Bravo Platform, make sure you are aware of the potential hazards and understand how to avoid being exposed to them.

Software version

This guide documents the following versions or later: Bravo Metabolomics Workbench 1.0 VWorks Automation Control 13.1.3 Bravo Diagnostics 19.1

Related guides

Use this guide in conjunction with the following guides: Automation Solutions Products General Safety Guide. Provides general safety

information and describes potential safety hazards that you might encounter when using Agilent Automation Solutions products. A copy of this safety guide is included with your shipment.

G5562A, G5563A Bravo Platform Safety and Installation Guide. Describes potential safety hazards and how to avoid them, how to install the Bravo Platform, and how to install the Light Curtain and shields. A copy of this safety guide is included with your shipment.

Bravo Platform User Guide. Explains how to set up, operate, and maintain the Bravo Platform and how to install accessories.

You can find the workbench user guides in the Literature Library of the Bravo Metabolomics Workbench software.

Contacting Agilent Technologies

Web: https://www.agilent.com Contact page: https://www.agilent.com/en/contact-us/page Documentation feedback: documentation.automation@agilent.com

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Bravo Metabolomics Workbench Utility description

Tip Transfer Utility Guide

Utility description

You can use the Tip Transfer utility to prepare the tip boxes for the Bravo Metabolomics Workbench applications. The Tip Transfer v2.0 utility moves 112 columns of Agilent 250-L pipette tips from a source tip box into an empty tip box. This utility is designed to transfer contiguous, full columns of tips, where each column has eight pipette tips.

Before you start

Hardware requirements

The Tip Transfer utility requires the Bravo Metabolomics Sample Prep Platform. For a description of the components, see the Bravo Metabolomics Sample Prep Platform Getting Started Guide in the Literature Library of the Bravo Metabolomics Workbench.

Labware requirements

CAUTION Using a labware type at a deck location other than an approved labware option can cause a collision resulting in equipment damage. Ensure that you use only an approved labware option for each deck location.

The Tip Transfer utility requires Agilent 96 250-L pipette tips (part number 19477.002): Full tip box at deck location 5 Empty tip box at deck location 8 The following figure shows the Bravo deck locations for labware.

Figure Tip Transfer utility labware locations on the Bravo deck (top view)

Bravo Metabolomics Workbench Accessing the utility

4 Tip Transfer Utility Guide

Accessing the utility

To open the utility: In the App & Utility Libraries page of the Bravo Metabolomics Workbench, locate Tip Transfer, and then click Open. The Tip Transfer form opens.

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Bravo Metabolomics Workbench Running the protocol

Tip Transfer Utility Guide

Running the protocol

Before you start

On the Bravo deck, ensure the required labware is fully seated within the platepads on the deck: A tip box full of pipette tips is at deck location 5. An empty tip box is at deck location 8. The tip box lids are removed. All other deck locations are empty.

CAUTION A collision can occur if any pipette tips are present in the destination tip box. Ensure that the destination tip box is empty.

IMPORTANT The Source Tip Box must contain full, contiguous columns of 8 pipette tips each, within the range of columns specified.

Selecting the settings in the form

To specify the settings and select the labware types: 1 Under Tip Box Settings, specify the following values:

Setting Description

Columns of pipette tips in the Source Tip Box

Specifies the range of pipette tips initially present in the Source Tip Box before the run starts. In the left box, type the number of the first column of pipette tips

present in the tip box. In the right box, type the number of the last column of pipette tips

present in the tip box.

Columns to be filled in the Destination Tip Box

Sets the range of wells to be filled with pipette tips in the Empty Tip Box. In the right box, type the number of the last column in the tip box that will receive tips during the transfer. Note: The first column must start at A1.

Bravo Metabolomics Workbench Running the protocol

6 Tip Transfer Utility Guide

CAUTION If the Source Tip box column selection is greater than the actual number of pipette tips used, the Bravo Platform will apply too much force when mounting the tips. Likewise, if the column selection is less than the actual number of tips used, the Bravo Platform will not apply enough force to seat the tips properly. To prevent potential equipment damage, ensure that the column selection is correct.

Starting the protocol run

To start the protocol run: 1 Review the selections in the protocol form to confirm they are correct. Verify that

the physical layout on the Bravo deck matches the Deck Layout image in the form.

2 Make sure the tip boxes are properly seated within the platepads on the Bravo deck

CAUTION Use only the labware specified, and place them at the Bravo deck locations specified in the instructions. Using different labware or placing labware at unapproved deck locations can cause a collision resulting in equipment damage.

CAUTION Improperly seated labware can cause a hardware collision, resulting in equipment damage. Ensure that all labware are properly seated within the alignment features of their respective platepads.

3 Click to start the protocol run.

If this is the first time the utility has been run after powering up the Bravo Platform, the device initialization process begins. Proceed to step 4.

If the platform is already initialized, skip to step 6.

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Bravo Metabolomics Workbench Running the protocol

Tip Transfer Utility Guide

4 If the Bravo Error message appears stating There appears to be a plate present, verify that Bravo gripper is not holding labware, and then click Ignore and Continue, leaving device in current state to continue the initialization.

5 If the Please verify that it is safe to home the W-axis message appears, click Retry to continue homing the pipetting axis (w-axis).

When the initialization process is finished, the orange lights on the Bravo Platform light panel flicker briefly and then begin to flash.

Bravo Metabolomics Workbench Running the protocol

8 Tip Transfer Utility Guide

6 If an Error message appears, the software detected a conflict in the protocol setup. a Follow the on-screen instructions to click Pause and Diagnose and then click

Abort process in the Scheduler Paused dialog box. b Resolve the conflict described in the error message, for example, insufficient

number of source pipette tips for the requested transfer.

c Click to restart the run.

To monitor the progress of the run, check the Status area of the form.

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Bravo Metabolomics Workbench Running the protocol

Tip Transfer Utility Guide

About stopping or pausing a run

IMPORTANT Attempting to pause a running protocol to change a setting can be detrimental to the protocol. If you need to change a setting in a protocol that is actively running, pause the protocol, select Abort process from the Scheduler Paused dialog box, change the setting, and then restart the protocol.

For more detailed instruction, see the Bravo Metabolomics Sample Prep Platform Getting Started Guide in the Literature Library of the Bravo Metabolomics Workbench.

Cleaning up

When the protocol run is finished, make sure you remove all labware from the Bravo deck.

Bravo Metabolomics Workbench Automation movements during the protocol

G5589-90004 Revision A, December 2018

Copyright Agilent Technologies, Inc. 2018 All rights reserved

Automation movements during the protocol

This section describes the basic automation movements of the Bravo Platform during the Tip Transfer run.

Protocol step Head moves to deck location...

Action

Pick Up Tips 5 Presses on all the pipette tips in the Source Tip Box. Note: The Bravo head is centered over the tip box to press on tips even if the tip box is only partially full.

Eject Tips 8 Ejects the pipette tips into the empty Destination Tip Box at deck location 8. Note: The Bravo head is centered over the tip box to eject all the pipette tips.

Remove Excess Tips 8 If applicable, picks up any columns of excess tips from the Destination Tip Box. Note: The Bravo head is offset instead of centered above the tip box, when picking up excess columns of pipette tips. The number of excess tips is calculated from the Application Settings. If the number of columns of tips in the Source Tip Box matches the number of columns of tips to be transfered into the Destination Tip Box, this step is skipped.

Return Excess Tips to S

Manualsnet FAQs

If you want to find out how the Brave Metabolomics Workbench Agilent works, you can view and download the Agilent Brave Metabolomics Workbench Handler User Guide on the Manualsnet website.

Yes, we have the User Guide for Agilent Brave Metabolomics Workbench as well as other Agilent manuals. All you need to do is to use our search bar and find the user manual that you are looking for.

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