Contents

Agilent 96 Channel Wash Station Handler Maintenance Guide PDF

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Summary of Content for Agilent 96 Channel Wash Station Handler Maintenance Guide PDF

This guide contains the following topics: About this guide on page 2 About the wash station on page 4 When to replace the chimneys on page 6 Replacing the chimneys on page 7 AssayMAP Bravo: Verifying the wash station teachpoint on page 11 AssayMAP Bravo: Testing the wash station on page 22 Testing the wash station in a Bravo Platform on page 24 Replacing a wash station on page 28 Emptying the wash station on page 29 Uninstalling the wash station on page 33 Installing the wash station and connecting the tubing on page 34 Troubleshooting problems with the wash station on page 38 Measuring your chimney to verify type on page 40

Bravo Platform

96 Channel Wash Station Maintenance Guide

About this guide

2 Bravo 96 Channel Wash Station Maintenance Guide

About this guide

Assumptions

The instructions in this guide assume that you have the following: Experience working on mechanical equipment An understanding of the Bravo Platform Familiarity with the VWorks software

What this guide covers

This guide describes how to inspect and replace chimneys in the following Agilent wash station models.

Wash station name, Agilent part number (internal part number)

Description Liquid-handling head compatibility

AssayMAP cartridge compatibility

96 Channel Wash Station, G5498B#90 (G5409-60125)

Equipped with 96 wide-bore chimneys (part number G5409-68004) and a bracketed platepad to secure it. This model supersedes the following wash station models listed in this table. Note: Use only the wide-bore chimneys with this wash station model.

Bravo 96AM Head, and 96LT, 96ST, and 384ST heads

5 L or 25 L cartridges

96AM Wash Station, G5498B#57 (11961-311)

Equipped with 96 standard-bore chimneys (part number 15586-002) before June 2020 or 96 wide-bore chimneys (part number G5409-68004) after June 2020. This model also has a bracketed platepad to secure it. This wash station may be retrofitted with the wide-bore chimneys (part number G5409-68004).

Bravo 96AM Head, and 96LT, 96ST, and 384ST heads

5 L cartridges 25 L cartridges only if upgraded with white, wide- bore chimneys

96 Well MicroWash Reservoir, G5498B#51 (11961-301)

Equipped with 96 standard-bore chimneys (part number 15586-002). The setscrews in the deck platepads are used to secure this wash station in place.

96LT, 96ST, and 384ST heads

Incompatible Using this wash station on the AssayMAP Bravo Platform can cause a head crash.

3

About this guide

Bravo 96 Channel Wash Station Maintenance Guide

Software versions

The procedures in this guide describe the following software versions: Protein Sample Prep Workbench 3.2 and VWorks 13.1.5 or later Protein Sample Prep Workbench 4.0 and VWorks 14.1 or later

Related user guides and where to find them

You can access the Bravo user documentation in the VWorks Knowledge Base using any of the following methods: From within VWorks software, select Help > Knowledge Base or press F1.

From the Windows 10 desktop, select Start > All Apps > Agilent Technologies > VWorks Knowledge Base.

Go to www.agilent.com/chem/askb. For AssayMAP user documentation, see the Literature Library page in the Protein Sample Prep Workbench.

Contacting Agilent Technologies

Web: https://www.agilent.com Contact page: https://www.agilent.com/en/contact-us/page Documentation feedback: documentation.automation@agilent.com

For information on See

Potential safety hazards of the platform and how to avoid them Installing and setting up the Bravo Platform

G5562A, G5563A Bravo Platform Safety and Installation Guide

Installing and setting up the AssayMAP Bravo Platform

AssayMAP Bravo Platform Installation Guide

How to use Bravo Diagnostics Bravo Platform User Guide

About the wash station

4 Bravo 96 Channel Wash Station Maintenance Guide

About the wash station

Overview

The wash station is a component of the Bravo autofilling station, as the following figure shows.

Figure Example of autofilling station: wash station, Pump Module, tubing, and carboys

How liquid flows through the wash station

Fresh wash liquid enters the wash station through two inlet ports (1), shown in the following figure. The wash liquid flows up through the chimneys, and the waste overflows from the chimneys and is removed through two outlet ports (2).

Figure Wash station (1) inlet ports and (2) outlet ports

2 1

5

About the wash station

Bravo 96 Channel Wash Station Maintenance Guide

Types of wash station chimneys for 96 channel wash stations

The following figure and table describe the two types of wash station chimneys: Wide-bore chimneys. The 96 Channel Wash Station (part number G5409-60125)

comes equipped with the wide-bore chimneys. Standard-bore chimneys (formerly, large-bore chimneys). The 96AM Wash Station

(part number 11961-311) were previously equipped with the standard-bore chimneys, but as of June 2020 they ship with the wide-bore chimneys. The 96 Well MicroWash Reservoir/Tip Wash Station (part number 11961-301) ships with the standard-bore chimneys.

Note: If you are unsure of the chimney type that you have in the wash station, see Measuring your chimney to verify type on page 40.

Feature Wide-bore chimney Standard-bore chimney

Part number Agilent G5409-68004 (pack of 100) Agilent 15586-002 (pack of 100)

Color White, opaque Neutral, semi-translucent

Geometry Tapered diameter with wider top opening and narrower bottom opening.

Very slight taper from top to bottom opening.

Correct chimney orientation

Wider opening at top end; white bump for alignment closer to top end

White dot for alignment closer to top end

Liquid-handling head compatibility

All Agilent 96-channel heads and pipette tips

All Agilent 96-channel heads and pipette tips

AssayMAP cartridge compatibility

AssayMAP 25-L and 5-L cartridges AssayMAP 5-L cartridges only

When to replace the chimneys

6 Bravo 96 Channel Wash Station Maintenance Guide

When to replace the chimneys

The wash station chimneys require replacement for the following reasons: Irregular or damaged chimneys. See the following figure for examples. Incompatibility with AssayMAP 25-L cartridges. The standard-bore chimneys are

not compatible with the AssayMAP 25-L cartridges. Before using the 25-L cartridges, replace the standard-bore chimneys with the white wide-bore chimneys. See Types of wash station chimneys for 96 channel wash stations on page 5.

CAUTION To avoid a hardware crash and equipment damage, ensure that the wash station contains white wide-bore chimneys when using the AssayMAP 25 L cartridges.

Determining if the chimneys are in good condition

Carefully inspect the chimneys in the wash station to ensure that they are: In good condition and free of deformities and irregularities. See the following figure

for examples. Chimney irregularities or damaged chimneys can prevent wash solution from forming domes atop the chimneys, which will decrease the ability to wick off the droplets from the syringe probes of the Bravo 96AM Head and may introduce variability in the assay.

Of equal height. When the chimneys are fully seated in the wash station their height should be consistent (less than 1 mm difference in height). If any chimneys seem higher than the others, push them down to the hard stop. See step 8 in the following replacement procedure.

Installed in the correct orientation. See Types of wash station chimneys for 96 channel wash stations on page 5. Note: Incorrect orientation is only possible on the standard-bore chimneys.

Clean and free of any debris.

Figure Close up views of wash station chimneys

Standard-bore chimneys: Good condition Damaged standard-bore chimneys (possibly from a head collision)

7

Replacing the chimneys

Bravo 96 Channel Wash Station Maintenance Guide

Replacing the chimneys

WARNING The probes of the AssayMAP Bravo 96AM Head are sharp and can scratch you if they brush across your hand. A probe scratch can expose you to any contaminants remaining on the probes. Wear gloves and use extreme caution to avoid brushing against the probes.

Before you start

Required tools and materials Replacement chimneys and chimney insertion tool (included in the package)

Ensure that you have the correct chimney type. See Types of wash station chimneys for 96 channel wash stations on page 5. To verify the chimney type installed in your wash station, see Measuring your chimney to verify type on page 40. Note: If you do not have an insertion tool, use a rubber pad between your thumb and the chimney to protect your thumb while pressing down on each chimney.

Needle-nose pliers to remove the old model or defective chimneys from the wash station.

IMPORTANT Ensure that all chimneys are of the same type, wide-bore or standard-bore. Ensure you discard any chimneys that you remove. Using pliers on a chimney will permanently damage the chimney.

IMPORTANT Use only wide-bore chimneys with the 96 Channel Wash Station (part number (G5409-60125).

Workflow Step For this task... See...

1 Inspect the chimneys for damage and irregularities.

Determining if the chimneys are in good condition on page 6

2 Replace the chimneys. Replacing the chimneys on page 7

3 AssayMAP Bravo Platform. Verify the teachhpoint at the wash station and adjust it as necessary.

AssayMAP Bravo: Verifying the wash station teachpoint on page 11

Bravo Platform. If you replace standard-bore chimneys with wide-bore chimneys, verify the x- and y-axes of the teachpoint at the wash station.

Bravo Platform User Guide

Replacing the chimneys

8 Bravo 96 Channel Wash Station Maintenance Guide

Chimney replacement procedure

To replace the chimneys: 1 Exit the Protein Sample Prep Workbench and VWorks software. 2 On the side of the Bravo Platform, press the power switch to the off (o) position. 3 Gently, move the Bravo head manually to a deck location that allows you

unobstructed access to the wash station installed on the deck. For example, if the wash station is installed at deck location 1, move the head to the far right side over deck location 3, as the following figure shows.

Figure Bravo head positioned at deck location 3

4 Using needle-nose pliers, pull out the defective (or older model) chimney from the wash station.

IMPORTANT Ensure that you discard any chimneys that you remove from the wash station. Using pliers to remove the chimneys will damage them.

5 Orient the new chimney with the little alignment bump (wide-bore chimney) or dot (standard-bore chimney) as the figure shows, and then insert the chimney bottom end into the hole in the wash station. Note: All the chimney alignment bumps or dots should face the same direction.

4 Start up the platform and verify that all the chimneys work consistently using one of the following procedures:

AssayMAP Bravo Platform AssayMAP Bravo: Testing the wash station on page 22

Bravo Platform Testing the wash station in a Bravo Platform on page 24

Step For this task... See...

Bravo

9

Replacing the chimneys

Bravo 96 Channel Wash Station Maintenance Guide

CAUTION To avoid a hardware crash and equipment damage, ensure that the chimneys are oriented correctly in the wash station.

6 Position the (1) insertion tool atop the (2) chimney, and press straight down on the insertion tool to install the chimney until you feel a hard stop. The chimney touches the bottom surface of the wash station when fully seated.

CAUTION Use care while pressing down in a vertical motion to avoid bending the chimneys during installation.

Wide-bore chimney orientation Standard-bore chimney orientation

Alignment bump faces the front of the wash station. Larger opening is at the top. This is the only orientation that will fit.

Little dot on side of chimney is at the top and faces wash station rear (side with ports). Even though this chimney fits in either orientation, the size of the top and bottom ends are different.

Front

Bump Dot

Rear

1

2

Replacing the chimneys

10 Bravo 96 Channel Wash Station Maintenance Guide

7 Repeat steps 1 to 5 to replace any other chimneys. 8 Carefully inspect the chimneys in the wash station to ensure proper alignment:

All the chimney tops are level with each other (less than 1 mm difference in height). If any chimney is taller than the others, use the insertion tool or your thumb to press down on the chimney so that it touches the bottom surface (hard stop) of the wash station.

All the alignment bumps or dots on the chimneys are facing the same direction.

Figure Example of wide-bore chimney alignment: tops level and alignment bumps aligned

IMPORTANT Good condition chimneys and consistent chimney height are critical for proper functioning of the wash station.

Bravo

11

AssayMAP Bravo: Verifying the wash station teachpoint

Bravo 96 Channel Wash Station Maintenance Guide

AssayMAP Bravo: Verifying the wash station teachpoint

The syringes in the Bravo 96AM Head are fragile and can be damaged if the head collides with an object on the deck. To prevent the potential of collisions at the wash station, you must verify the teachpoint at the wash station after installing new chimneys. For this procedure, you will determine if the alignment of the syringe probes in the Bravo 96AM Head are still centered in wash station chimneys. If an adjustment is necessary, only the x- and y-axes will be adjusted.

Before you start

Ensure the following: The emergency-stop pendant is within easy reach.

No pipette tips or cartridges are on the syringes of the head.

WARNING The Jog/Teach tab in Bravo Diagnostics poses an increased risk of collision because the jog controls enable you to move the head without regard to items on the Bravo deck. To prevent potential injury and damage to the device, use extreme caution when using the Jog/Teach tab controls.

WARNING The red Stop motors button in Bravo Diagnostics does not perform an immediate stop. To perform an emergency stop, press the red button on the emergency-stop pendant.

Workflow

Workflow for verifying and adjusting the teachpoints Step For this task... See...

1 In the Bravo Diagnostics dialog box, initialize the AssayMAP 1 profile.

Initializing AssayMAP 1 profile on page 12

2 Verify the wash station teachpoint for the AssayMAP 1 profile, and adjust it if necessary.

Verifying and adjusting the teachpoint for AssayMAP 1 profile on page 14

AssayMAP Bravo: Verifying the wash station teachpoint

12 Bravo 96 Channel Wash Station Maintenance Guide

Initializing AssayMAP 1 profile

1 Ensure that the Protein Sample Prep Workbench and VWorks software is closed.

2 To start the VWorks software, click the VWorks icon on the desktop and log

in using your user name and password. 3 On the VWorks toolbar, ensure that Simulation is off is displayed.

4 In the VWorks window, click File > Open. 5 In the Open File dialog box, ensure the file type is Device Files (*.dev), select

AssayMAP Bravo.dev, and then click Open: Workbench 3.2. The file is stored at

C:\VWorks Workspace\Workbench\Device Files\ Workbench 4.0. The file is stored in Shared Services storage:

VWorks Plus stores the file at /VWorks Projects/VWorks/Protein Sample Prep Workbench/Device Files/

VWorks Standard stores the file at C:\OLSS Projects\VWorks Projects\VWorks\Protein Sample Prep Workbench\Device Files\

6 Click No when the Would you like to initialize devices now? message appears.

7 In the Devices tab (AssayMAP Bravo.dev), select the device and then click Device diagnostics.

3 If you updated the AssayMAP 1 profile, update the remaining profiles:

Protein Sample Prep Workbench 3.2 and VWorks 13.1.5 13.1.x

Updating the remaining profiles in VWorks 13.1.x on page 17

Protein Sample Prep Workbench 4.0 and VWorks 14.1 or later

Updating the remaining profiles in VWorks 14.1 or later on page 20

Step For this task... See...

13

AssayMAP Bravo: Verifying the wash station teachpoint

Bravo 96 Channel Wash Station Maintenance Guide

8 In the Agilent Bravo Diagnostics dialog box, click the Profiles tab, and then do the following: a Verify the Profile name is AssayMAP 1, and verify the Teaching tip type is

250 L. b Click Initialize this profile.

AssayMAP Bravo: Verifying the wash station teachpoint

14 Bravo 96 Channel Wash Station Maintenance Guide

Verifying and adjusting the teachpoint for AssayMAP 1 profile

To verify and, if necessary, adjust the teachpoint: 1 In Agilent Bravo Diagnostics, click the Jog/Teach tab, and then click Location 1.

Note: Additional accessories will appear in the image if you have configured them in this profile.

2 In the Location list, locate the Z-axis box. Write down the Z-axis value on a piece of paper. You will use this value in a subsequent step. Note: The Z-axis teachpoint value for deck location 1 is typically between 105.2105.8 mm for a standard height AssayMAP Bravo Platform and standard platepad.

15

AssayMAP Bravo: Verifying the wash station teachpoint

Bravo 96 Channel Wash Station Maintenance Guide

3 Move the head down so that you can verify the teachpoint as follows: a In the Approach height above teachpoint box, type 50, and then click Approach.

b Carefully, use the Jog Axes controls to move the head down (z-axis) using small incremental steps until the tips of the probes are approximately 1 mm above the tops of the chimneys. To do this: Set the jog increment (mm), and then click the Down +Z button. Reduce the increment value as the tips get closer to the wash station chimneys.

4 While syringe probe tips are in position just above the chimney tops, check the alignment to see if the probe tips are centered in the chimneys: If no adjustment is necessary to center the probe tips, click Move to safe height

at current X/Y position and then click Cancel to close Bravo Diagnostics without saving any changes. Proceed to AssayMAP Bravo: Testing the wash station on page 22.

If an adjustment is necessary to center the probe tips, continue to step 5. 5 Carefully, use the Jog Axes controls to position the syringe probes so that they are

centered over the corresponding chimneys in the x- and y-axes.

AssayMAP Bravo: Verifying the wash station teachpoint

16 Bravo 96 Channel Wash Station Maintenance Guide

6 When the probes and chimneys are aligned, use the Jog Axes controls to move the head down incrementally along the z-axis, using smaller and smaller jog increments until you reach the original Z-axis teachpoint value that you recorded in step 2. Note: This step will insert the probes into the wash station chimneys, which is a normal part of the teaching process.

7 When the position of the z-axis (as indicated by the Z-axis readout in the bottom right of the screen) matches the Z-axis value that you recorded in step 2, click Teach to set the new teachpoint for deck location 1.

8 Click Move to safe height at current X/Y position. The head moves up and returns to a safe height above the wash station.

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AssayMAP Bravo: Verifying the wash station teachpoint

Bravo 96 Channel Wash Station Maintenance Guide

9 In the Profiles tab, click Update this profile to save the new teachpoint.

10 VWorks Plus only. If an audit trail is being logged, the Audit Comment dialog box opens. Select or type the audit comment, and then click OK.

Updating the remaining profiles in VWorks 13.1.x

The AssayMAP Teachpoint Update protocol will set the teachpoints for all other profiles in the Protein Sample Prep Workbench based on the AssayMAP 1 profile. Note: The AssayMAP Teachpoint Update protocol also automatically makes z-axis teachpoint adjustments for the GlykoPrep-Plus profile to accommodate the height of the Red PCR Insert at deck location 4, and the AssayMAP Normalization profile to accommodate the height of the Bravo Plate Riser at deck locations 2 and 6.

Before you start Ensure that you have the AssayMAP Teachpoint Update protocol (.pro), which is installed in the following location: C:\VWorks Workspace\Workbench\Protocols\AssayMAP Teachpoint Update v1p0.pro Note: The AssayMAP Teachpoint Update protocol is not designed to work in simulation mode.

To start the AssayMAP Teachpoint Update protocol: 1 In the VWorks window, click File > Open. The Open dialog box appears. 2 In the Open dialog box, ensure the file type is Protocol Files (*.pro). Locate the

C:\VWorks Workspace\Workbench\Protocols folder, select the AssayMAP Teachpoint Update v1p0.pro file, and then click Open.

AssayMAP Bravo: Verifying the wash station teachpoint

18 Bravo 96 Channel Wash Station Maintenance Guide

3 When the following VWorks initialization message appears, click Yes.

The AssayMAP Bravo.dev file opens, and the AssayMAP 1 profile initializes. 4 Ensure that the AssayMAP Teachpoint Update protocol is open in the VWorks

window, and then click Start.

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AssayMAP Bravo: Verifying the wash station teachpoint

Bravo 96 Channel Wash Station Maintenance Guide

5 In the Run Configuration dialog box, click Finish to start the protocol.

6 When the Update Teachpoints dialog box appears, click Continue to update teachpoints in all the other Workbench profiles based on the AssayMAP 1 profile.

7 When the WARNING dialog box appears, click Continue to complete the update.

8 Close the VWorks software.

AssayMAP Bravo: Verifying the wash station teachpoint

20 Bravo 96 Channel Wash Station Maintenance Guide

Updating the remaining profiles in VWorks 14.1 or later

This procedure updates the following teachpoints: Pipette head teachpoints (x- , y-, and z-axes) for all nine deck locations Gripper y-axis offset Note: The AssayMAP Normalization profile for Workbench 4.0 includes z-axis offsets configured at deck locations 2 and 6 to accommodate the height of the Bravo Plate Riser.

To copy the AssayMAP 1 teachpoints to the other profiles: 1 In the Bravo Diagnostics dialog box, ensure that the AssayMAP 1 profile is initialized

in the Profiles tab. 2 In the Jog/Teach tab, under Advanced operations, select Copy teachpoints to other

Profiles, and then click Perform advanced operation.

A message appears and warns you that the software must inspect every Bravo profile before providing a list of eligible profiles. Click Yes to proceed.

3 In the Copy teachpoints to other Profiles dialog box, select the following profiles, and then click OK: AssayMAP Normalization AssayMAP Peptide Sample Prep Single Plate InSolution Digestion v2

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AssayMAP Bravo: Verifying the wash station teachpoint

Bravo 96 Channel Wash Station Maintenance Guide

4 VWorks Plus only. If an audit trail is being logged, the Audit Comment dialog box opens. Select or type the audit comment for all the profiles to be updated, and then click OK.

5 Click OK when the confirmation message appears.

AssayMAP Bravo: Testing the wash station

22 Bravo 96 Channel Wash Station Maintenance Guide

AssayMAP Bravo: Testing the wash station

Before you start

Ensure that you verify the wash station teachpoint and adjust it as required. For details, see AssayMAP Bravo: Verifying the wash station teachpoint on page 11.

Starting up the AssayMAP Bravo Platform

To test the performance of the wash station, you run the Startup utility in the Protein Sample Prep Workbench. During the Startup protocol, carefully observe the fluid as it flows out of the top of the chimneys in the wash station.

To start up the system: 1 Check the levels of the wash station source and waste carboys, and fill or empty as

required. 2 Turn on the AssayMAP Bravo Platform and accessories. 3 Start the Protein Sample Prep Workbench, and open the Utility Library.

4 Open the System Startup/Shutdown utility .

5 Workbench 4.0 only. Under Experiment Settings, select the following:

a If applicable, click Select Experiment. The Experiments Editor opens.

Select the Experiment ID that you want to use to capture the steps in this application or utility, and then click Use Selected. The Experiments Editor closes.

b In the form, click Select Method to locate and select a method. In the Open File dialog box, select the method, and click Open. To modify the selected method, proceed to step c. Otherwise, go to step 6.

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AssayMAP Bravo: Testing the wash station

Bravo 96 Channel Wash Station Maintenance Guide

c To create or modify a method: VWorks Plus only. Administrator or technician privileges are required to create or modify methods. In the Startup Options area of the AssayMAP Startup & Shutdown form, enter the values for the following properties:

To save the method, click Save Method. In the Save File As dialog box, type the file name and click Save. VWorks Plus only. You must save the method before you can run it.

Note: If the wash station was allowed to dry, change the Wash Station Prime Duration setting from 10 to 60 seconds before clicking Run Startup.

6 Click Run Startup to initialize the AssayMAP Bravo Platform and accessories.

WARNING When you initialize the Bravo Platform, the head and tie bar will move. To prevent injury, keep clear of the device while it is in motion.

7 During the Startup protocol, verify that all the wash station chimneys have liquid flowing through them. If all the chimneys are flowing evenly, the device is ready for operation. If no liquid is flowing through any of the chimneys, check to see if:

Liquid is in the source carboy. The tubing is clamped into the pump heads. The tubing is routed correctly, and free of kinks or damage. Repeatedly run the Startup utility or increase the wash station prime duration in the Startup utility. For more guidelines, see Troubleshooting problems with the wash station on page 38.

If no liquid is flowing through a subset of chimneys, suspect an airlock. Use a plastic transfer pipette or a 1 mL pipette to draw liquid through the problematic chimneys. Rerun the Startup utility, and repeat this step until all the chimneys are flowing freely.

Property Description Number of Syringe Wash Cycles

Specifies the number of syringe wash cycles that will be conducted at the wash station at deck location 1. Default: 3 Range: 010

Wash Station Prime Duration (sec)

Specifies how long (seconds) to run the wash station pumps in order to prime the tubing lines and wash station chimneys. If the tubing lines are dry, set this value to 60 seconds. Default: 10 Range: 0300

Testing the wash station in a Bravo Platform

24 Bravo 96 Channel Wash Station Maintenance Guide

Testing the wash station in a Bravo Platform

Before you start

If you did not uninstall the wash station, you do not need to verify the teachpoints for the wash station installed on a standard Bravo Platform. If you replaced the standard- bore chimneys in the wash station with wide-bore chimneys, you should verify the teachpoint at the wash station.

CAUTION If you uninstall and reinstall the wash station, the wash station teachpoint can shift. To prevent a potential crash, verify the wash station teachpoint in your Bravo profiles any time you reinstall the wash station. In addition, you should verify the wash station teachpoint if you replace standard-bore chimneys with wide-bore chimneys.

For instructions on how to set and verify the Bravo teachpoints, see Bravo Platform User Guide. To test the performance of the wash station, use the following procedure to run the Pump Reagent task in Bravo Diagnostics to pump liquid into the wash station. While running the task, carefully observe the fluid as it flows out of the top of the chimneys in the wash station. Verify that all the wash station chimneys have liquid flowing through them.

Starting up the Bravo Platform

To start up the system: 1 Check the levels of the wash station source and waste carboys, and fill or empty as

required. 2 Turn on the Bravo Platform and accessories. 3 Start the VWorks software. 4 In the VWorks window, ensure that Simulation is off.

5 In the VWorks window, open the Bravo device file. To do this, click File > Open. In the Open File dialog box, ensure the file type is Device Files (*.dev), select the file, and then click Open. VWorks 13.1.x. Typically, the file is stored at

C:\VWorks Workspace\Workbench\Device Files\ VWorks 14.1. The VWorks project folder path varies depending on the VWorks

edition and how the folders are configured within the VWorks project, for example:

VWorks edition Example Devices folder path VWorks Plus /VWorks Projects/VWorks/Devices

25

Testing the wash station in a Bravo Platform

Bravo 96 Channel Wash Station Maintenance Guide

6 Click No when the Would you like to initialize devices now? message appears.

7 Open Bravo Diagnostics as follows: In the VWorks window, select the device in the Device tab (.dev), and click Device diagnostics.

The Agilent Bravo Diagnostics dialog opens.

VWorks Standard C:\OLSS Projects\VWorks Projects\VWorks\Devices VWorks edition Example Devices folder path

Testing the wash station in a Bravo Platform

26 Bravo 96 Channel Wash Station Maintenance Guide

8 To initialize your Bravo profile, click the Profiles tab, select the Profile name, and then click Initialize this profile.

WARNING When you initialize the Bravo Platform, the head and tie bar will move. To prevent injury, keep clear of the device while it is in motion.

9 If the There appears to be a plate present error message appears, click Ignore and Continue to continue the homing process.

10 When the Verify that it is safe to home the W-axis message appears, click Ignore to skip the w-axis homing for now.

27

Testing the wash station in a Bravo Platform

Bravo 96 Channel Wash Station Maintenance Guide

Running the Pump Reagent task to fill the wash station

To run the Pump Reagent task: 1 In Bravo Diagnostics, click the Processes tab.

2 Select the following:

3 Click Execute command. 4 Observe the fluid as it flows out of the top of the chimneys in the wash station. If all

the chimneys are flowing evenly, the wash station is ready for operation. To troubleshoot problems with the wash station, locate your problem in the troubleshooting table and try the solution. See Troubleshooting problems with the wash station on page 38. If the problem persists, contact Agilent Technical Support.

IMPORTANT To home the w-axis, make sure that you reinitialize the profile before running a protocol.

Setting Value Location Number of the deck location where

the wash station is installed. Labware at selected location 96AM Tip Wash Station Command to execute Pump Reagent Pump Reagent properties

Reservoir mode Fill Pump speed (0100%) 50% Pump on time (1600 s) 60 seconds How often 1 Run second pump Yes Second pump speed (0100%) 60% Use weigh station No

Replacing a wash station

28 Bravo 96 Channel Wash Station Maintenance Guide

Replacing a wash station

If the wash station requires replacement, use the following workflow.

Workflow

Step For this task... See...

1 Empty the wash station. Emptying the wash station on page 29

2 Uninstall the wash station. Uninstalling the wash station on page 33

3 Install the wash station. Installing the wash station and connecting the tubing on page 34

4 Verify the wash station teachpoint and adjust it, as necessary, for your configuration:

AssayMAP Bravo Platform. Initialize the AssayMAP 1 profile and adjust the teachpoint for the wash station.

AssayMAP Bravo: Verifying the wash station teachpoint on page 11

Bravo Platform. Initialize your Bravo profile and verify the teachpoint for the wash station.

Bravo Platform User Guide

5 Start up the platform and verify that all the chimneys work consistently using one of the following procedures:

AssayMAP Bravo Platform AssayMAP Bravo: Testing the wash station on page 22

Bravo Platform Testing the wash station in a Bravo Platform on page 24

29

Emptying the wash station

Bravo 96 Channel Wash Station Maintenance Guide

Emptying the wash station

This topic describes how to use the Pump Reagent task in Bravo Diagnostics to empty any fluid from the wash station.

Before you start

WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they brush across your hand. A probe scratch can expose you to any contaminants remaining on the probes. Wear gloves and use extreme caution to avoid brushing against the probes.

WARNING When you initialize the Bravo Platform, the head and tie bar will move. To prevent potential injury, keep clear of the device while it is in motion.

CAUTION To prevent potential equipment damage, ensure that the deck is clear of any obstacles before initializing the Bravo Platform.

CAUTION Using an incorrect profile can cause equipment damage. Ensure that the profile is correct for the head type and deck configuration.

Running the Pump Reagent task to empty the wash station

To empty the wash station using the Pump Reagent task: 1 At the carboy that contains the wash solution, disconnect the lid and lift it so that

the tubing is above the liquid level in the carboy. 2 AssayMAP Bravo Platform only. Ensure that the Protein Sample Prep Workbench is

closed. 3 Start the VWorks software.

You can use the VWorks desktop icon or the Start menu on the Windows desktop to start the VWorks software.

4 In the VWorks window, ensure that Simulation is off.

5 In the VWorks window, open the device profile: To do this, click File > Open. In the Open File dialog box, ensure the file type is Device Files (*.dev), select the file, and then click Open. AssayMAP Bravo Platform only. Open the AssayMAP Bravo.dev file. Workbench 3.2. The file is stored at

C:\VWorks Workspace\Workbench\Device Files\

Emptying the wash station

30 Bravo 96 Channel Wash Station Maintenance Guide

Workbench 4.0. The file is stored in Shared Services storage: VWorks Plus stores the file at

/VWorks Projects/VWorks/Protein Sample Prep Workbench/Device Files/

VWorks Standard stores the file at C:\OLSS Projects\VWorks Projects\VWorks\Protein Sample Prep Workbench\Device Files\

6 Click No when the Would you like to initialize devices now? message appears.

7 In the Devices tab (for example, AssayMAP Bravo.dev), select the device and then click Device diagnostics.

Figure AssayMAP Bravo.dev tab and Profile setting for AssayMAP 1

The Agilent Bravo Diagnostics dialog opens.

31

Emptying the wash station

Bravo 96 Channel Wash Station Maintenance Guide

8 Click the Profiles tab, select the Profile name, and then click Initialize this profile. For the AssayMAP Bravo Platform, make sure that you select the AssayMAP 1 profile.

9 If the There appears to be a plate present error message appears, click Ignore and Continue to continue the homing process.

10 When the Verify that it is safe to home the W-axis message appears, click Ignore to skip the homing of the w-axis for now.

Emptying the wash station

32 Bravo 96 Channel Wash Station Maintenance Guide

11 Click the Processes tab and run the Pump Reagent task as follows:

a Select the following:

b Click Execute command. The wash station empties.

Setting Value Location Number of the deck location

where the wash station is installed For the AssayMAP Bravo Platform, the location is 1.

Labware at selected location 96AM Tip Wash Station Command to execute Pump Reagent Pump Reagent properties

Reservoir mode Fill Pump speed (0100%) 50% Pump on time (1600 s) 60 seconds How often 1 Run second pump Yes Second pump speed (0100%) 60% Use weigh station No

33

Uninstalling the wash station

Bravo 96 Channel Wash Station Maintenance Guide

Uninstalling the wash station

IMPORTANT Exit the Protein Sample Prep Workbench and VWorks software before you turn off the Bravo Platform.

WARNING To prevent potential injury, turn off the Bravo Platform before you install or remove the wash station.

To uninstall the wash station: 1 On the side of the Bravo Platform, press the power switch to the off (o) position. 2 Gently, move the Bravo head manually to position it so that you have unobstructed

access to the wash station installed on the deck. For example, if the wash station is installed at deck location 1, move the head to the far right over deck location 3, as the following figure shows.

3 On the left side as you face the front of the Bravo Platform, loosen the two 2-mm screws using a hex driver. Uninstall the bracket that secures the wash station to the platepad.

Figure Wash station secured with platepad brackets

Bravo

Installing the wash station and connecting the tubing

34 Bravo 96 Channel Wash Station Maintenance Guide

4 On the opposite side of the platepad, loosen but do not remove the screws that secure the remaining bracket. Guide the wash station horizontal slot off the platepad bracket.

5 Lift the wash station off of the platepad. For ease of handling, you may disconnect the tubing from the wash station using the quick-disconnect fittings.

Installing the wash station and connecting the tubing

CAUTION On the AssayMAP Bravo Platform, ensure that you use either the 96 Channel Wash Station (part number G5409-60125) or the 96AM Wash Station. Using a different wash station model can result in a potential collision with the Bravo 96AM Head.

Installing the wash station

Before you start

WARNING To prevent potential injury, turn off the Bravo Platform before you install or remove the wash station.

The following procedure assumes that the platepad with brackets is already installed on the Bravo deck at deck location 1.

Figure Wash station installed at deck location 1

Bravo

35

Installing the wash station and connecting the tubing

Bravo 96 Channel Wash Station Maintenance Guide

To install the wash station: 1 At the wash station deck location, ensure that the outer side bracket (next to the

crosshairs) is removed from the platepad, or that the screws securing the bracket are loosened.

Figure Bracket next to platepad crosshairs

Note: The wash station may be easier to install if you install one of the platepad brackets after the wash station is in position.

2 Install the wash station on the platepad as follows: a Ensuring that the inlet and outlet ports are towards the rear of the deck, place

the wash station on the platepad. b Guide the platepad bracket into the wash stations horizontal side slot. c Re-install the remaining platepad bracket. Ensure that you guide the bracket

into the corresponding horizontal slot on the wash station. Ensure that both brackets remain engaged as you tighten the bracket screws.

d Ensure that the wash station sits level on the platepad and that both brackets are holding the wash station securely in place.

Figure Wash station secured with platepad brackets

Installing the wash station and connecting the tubing

36 Bravo 96 Channel Wash Station Maintenance Guide

Tubing connections for the wash station

Reconnect any of the disconnected tubing. For reference, see the following figure. As the figure shows, the upper pump (A) is for the fill line and the lower pump (B) is for the empty line. Note: You specify which pump is for filling and which pump is for emptying in Bravo Diagnostics.

Figure Wash station fill line (A) and empty line (B)

To ensure proper chimney filling and waste emptying characteristics for the wash station, ensure that you use the proper tubing combination for the Pump Module. See the following figure and table. For a detailed tubing installation procedure, see the AssayMAP Bravo Platform Installation Guide or the Pump Module User Guide.

CAUTION To ensure that the tubing is not pinched by the pump head clamp, leave the clamps wide open and only tighten them if necessary. Make sure you push the tubing to the back of the pump head while closing the flip-top cover.

Figure Open pump head (side view) with fully open clamp and clamp dial

A

B Source Waste

37

Installing the wash station and connecting the tubing

Bravo 96 Channel Wash Station Maintenance Guide

Figure Tubing configuration

2

54 63 43 45

7 8

8

6

1

4 4 4

6

6 4

99

Item Component Description

1 Source bottle Supplies the fill liquid for the wash station.

2 Waste bottle Collects the waste liquid that is pumped from the reservoir.

3 Tubing, 8-mm (5/16-in)

The empty-line tubing in the lower pump head, which is used for emptying the wash station. The larger diameter tubing ensures that the fluid is removed efficiently from the wash station

4 Tubing, 6.4-mm (1/4-in)

The fill-line tubing from the source bottle to the wash station, and empty-line tubing connections on either side of the 5/16-in diameter tubing. Note: The 5/16-in diameter tubing is too large for the quick-disconnect fittings and the wash station ports. So, you use a union connector (5) to splice the 5/16-in tubing to the 1/4-in tubing.

5 Connector, union Joins the two sizes of tubing.

6 Quick-disconnect fitting

Enables easy removal of the wash station and quick replacement of the tubing in the pump heads. The quick-disconnect fittings include an automatic-close valve. Fluid can flow only if a positive connection is made.

7 Inline pump filter Removes the particulates that can clog the chimneys in the wash station.

8 3-way connector Enables one tube to branch into two tubes at the input and output ports on the wash station.

9 Quick-disconnect fitting

Acts as a vent to prevent pressure from building up inside the bottle. Plug a quick-disconnect fitting in the open hole in the carboy to act as a vent. If an extra quick-disconnect fitting is not available, loosen the lid to allow air to vent.

Troubleshooting problems with the wash station

38 Bravo 96 Channel Wash Station Maintenance Guide

Troubleshooting problems with the wash station

Problem Potential causes Solution

Liquid flow is constricted or insufficient in all the chimneys of the wash station.

The source carboy does not have any liquid.

Ensure that the source carboy is filled.

A pump head is not closed.

Ensure that the flip-top cover on each pump head is closed. To ensure that the tubing is not pinched by the pump head clamp, leave the clamps wide open and only tighten them if necessary. Make sure you push the tubing to the back of the pump head while closing the flip-top cover.

No liquid is in the tubing. Run the pumps for 30 to 60 seconds to drive liquid through the length of the tubing. For an AssayMAP Bravo Platform, run the Startup utility with a longer run time.

The tubing is not routed properly, the tubing is kinked or blocked, or the tubing is worn where it is clamped onto the pump head.

Inspect the tubing to verify that it is routed properly and in good condition. Check for kinks and damage to the tubing. Reroute and replace the tubing if necessary. If the tubing is worn where it is clamped onto the pump head, replace the tubing going through the pump head. To ensure that the tubing is not pinched by the pump head clamp, leave the clamps wide open and only tighten them if necessary. Make sure you push the tubing to the back of the pump head while closing the flip-top cover.

The tubing diameter of the fill line is the wrong size, or the pump head clamp is too tight for the tube size.

Replace the tubing on the fill line using the correct diameter tubing. See Tubing connections for the wash station on page 36. If necessary adjust the pump head clamp, see the Pump Module User Guide.

The inline filter is clogged. Replace the inline filter. See Tubing connections for the wash station on page 36.

Liquid is not flowing through a subset of the chimneys in the wash station.

The chimneys are blocked or damaged.

To resolve the problem chimneys:

1 Use a plastic Pasteur or 1 mL pipette to quickly aspirate liquid through the chimneys that are not flowing. Test the wash station again to verify that the chimneys are flowing. If the problem persists, repeat this step several times.

2 If the problem persists, replace the problematic chimneys. See Replacing the chimneys on page 7.

39

Troubleshooting problems with the wash station

Bravo 96 Channel Wash Station Maintenance Guide

Waste is accumulating in the wash station.

The waste carboy is not properly vented and the carboy lid is too tight causing pressure to build within the carboy.

Plug a quick-disconnect fitting into the open hole in the carboy to act as a vent and prevent pressure from building up inside the bottle. If an extra quick-disconnect fitting is not available, loosen the lid to allow air to vent.

A pump head is not closed.

Ensure that the flip-top cover on each pump head is closed.

The tubing is not routed properly, the tubing is kinked or blocked, or the tubing is worn where it is clamped onto the pump head.

Inspect the tubing to verify that it is routed properly and in good condition. Check for kinks and damage to the tubing. Reroute and replace the tubing if necessary. If the tubing is worn where it is clamped onto the pump head, replace the tubing in the pump head with fresh tubing. To ensure that the tubing is not pinched by the pump head clamp, leave the clamps wide open and only tighten them if necessary. Make sure you push the tubing to the back of the pump head while closing the flip-top cover.

The tubing diameter of the fill line is the wrong size, or the pump head clamp is too tight for the tube size.

Replace the tubing on the fill line using the correct diameter tubing. See Tubing connections for the wash station on page 36. If necessary adjust the pump head clamp, see the Pump Module User Guide.

Tubing creeps through the pump head instead of being secured in place.

The pump head clamp is too loose for the tube size.

Adjust the tubing clamp for the tube size. See the Pump Module User Guide.

The tubing is crushed or damaged in the pump head.

The pump head clamp is too tight for the tube size.

Adjust the tubing clamp for the tube size. See the Pump Module User Guide.

The fluid in the wash station chimneys is rapidly emptying when the pumps are not running.

The tubing is worn where it is clamped onto the pump head.

Replace the worn tubing in the pump head with fresh tubing.

Wash station chimneys are sticking to AssayMAP 25 L cartridges, or 25 L cartridges are sticking to the chimneys.

The wash station has the standard-bore chimneys and they are installed upside down.

To avoid a potential crash, ensure that the wash station contains white wide-bore chimneys if you are using the 25 L cartridges. See When to replace the chimneys on page 6. To avoid a potential crash, ensure that the chimneys are properly oriented in the wash station. See Replacing the chimneys on page 7.

Problem Potential causes Solution

Measuring your chimney to verify type

40 Bravo 96 Channel Wash Station Maintenance Guide

Measuring your chimney to verify type

If you are unsure of the chimney type that you have, you can use the following figure as a tool to measure a chimney. You can do this even while the chimney is installed in the wash station.

To check the chimney type: 1 Print out this page, and cut out the figure using the cut line as a guide. 2 Visually inspect the vertical surface of your chimney for the following:

Wide-bore chimney. Small white bump is located closer to the top end. Standard-bore chimney. Small white dot is located closer to the top end.

3 Align the dimension marks at the top of this drawing above the top opening of the actual chimney to confirm the size. The radius of the wide-bore chimney is larger that the standard-bore chimney. See the following figures for examples.

Standard-bore chimney

Wide-bore chimney

Bottom end

3.68 mm4.72 mm

Scale 1:1

White bump White dot

Cut line Cut line

41

Measuring your chimney to verify type

Bravo 96 Channel Wash Station Maintenance Guide

Figure Wide-bore chimney and drawing aligned atop chimney

Figure Standard-bore chimney and drawing aligned atop chimney

Standard-bore chimney

Wide-bore chimney

3.68 mm4.72 mm

White dot

Drawing aligned atop chimney Mismatch

Top of wide-bore chimney

White bump

Standard-bore chimney

Wide-bore chimney

3.68 mm4.72 mm

White dot

Top of standard-bore chimney

Drawing aligned atop chimney Mismatch

White bump

www.agilent.com Agilent Technologies, Inc. 2021

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