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Agilent 1260 Infinity Degasser User Manual PDF
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Summary of Content for Agilent 1260 Infinity Degasser User Manual PDF
Agilent Technologies
Agilent 1260 Infinity High Performance Degasser
User Manual
Notices Agilent Technologies, Inc. 2011-2013, 2014
No part of this manual may be reproduced in any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.
Manual Part Number G4225-90002
Edition 02/2014
Printed in Germany
Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn
This product may be used as a com- ponent of an in vitro diagnostic sys- tem if the system is registered with the appropriate authorities and com- plies with the relevant regulations. Otherwise, it is intended only for gen- eral laboratory use.
Warranty
The material contained in this docu- ment is provided as is, and is sub- ject to being changed, without notice, in future editions. Further, to the max- imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par- ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor- mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep- arate agreement shall control.
Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor- dance with the terms of such license.
Restricted Rights Legend If software is for use in the performance of a U.S. Government prime contract or subcon- tract, Software is delivered and licensed as Commercial computer software as defined in DFAR 252.227-7014 (June 1995), or as a commercial item as defined in FAR 2.101(a) or as Restricted computer soft- ware as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will
receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated condi- tions are fully understood and met.
WARNING
A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indi- cated conditions are fully under- stood and met.
1260 Infinity High Performance Degasser - User Manual
In This Guide
In This Guide
This manual covers the Agilent 1260 Infinity High Performance Degasser (G4225A).
1 Introduction
This chapter gives an introduction to the module, instrument overview and internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and performance specifications.
3 Installing the Degasser
This chapter gives information about the preferred stack setup for your system and the installation of your module.
4 Using the Degasser
This chapter explains how to use the module.
5 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional devices.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.
7 Maintenance
This chapter describes the maintenance of the module.
1260 Infinity High Performance Degasser - User Manual 3
In This Guide
8 Parts for Maintenance
This chapter provides information on parts for maintenance.
9 Identifying Cables
This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.
10 Hardware Information
This chapter describes the degasser in more detail on hardware and electronics.
11 Appendix
This chapter provides addition information on safety, legal and web.
4 1260 Infinity High Performance Degasser - User Manual
Contents
Contents
1 Introduction 7
Introduction to the Vacuum Degasser 8 System Overview 9
2 Site Requirements and Specifications 13
Site Requirements 14 Physical Specifications 17 Performance Specifications 18
3 Installing the Degasser 19
Unpacking the Vacuum Degasser 20 Optimizing the Stack Configuration 21 Installation Information on Leak and Waste Handling 24 Installing the Vacuum Degasser 28 Flow Connections to the Vacuum Degasser 31 Operational Hints for the Vacuum Degasser 34 Transporting the Vacuum Degasser 37
4 Using the Degasser 39
Leak and Waste Handling 40 When to Use a Vacuum Degasser 41 Prevent Blocking of Solvent Filters 42 Solvent Information 43
5 Optimizing Performance 49
Increasing the Degasser Performance and Degassing Level 50
6 Troubleshooting and Diagnostics 51
Overview of the Degasser's Indicators 52 Status Indicators 53 Module Status Indicator 54 Hardware Symptoms 55
1260 Infinity High Performance Degasser - User Manual 5
Contents
7 Maintenance 57
Warnings and Cautions 58 Introduction to Maintenance 60 Cleaning the Module 61 Assembling the Main Cover 62 Exchanging the Fuses of the Power Inline Filter 63
8 Parts for Maintenance 65
Cover Parts 66 Fuse 67 Accessory Kit 68
9 Identifying Cables 69
Cable Overview 70 Analog Cables 72 Remote Cables 74 BCD Cables 77 CAN Cable 79 External Contact Cable 80 Agilent Module to PC 81 Agilent 1200 Module to Printer 82
10 Hardware Information 83
Operating Principle of the 1260 Infinity High Performance Degasser 84 Electrical Connections 85 Interfaces 86
11 Appendix 93
General Safety Information 94 The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 97 Radio Interference 98 Sound Emission 99 Agilent Technologies on Internet 100
6 1260 Infinity High Performance Degasser - User Manual
1260 Infinity High Performance Degasser - User Manual
1 Introduction
Introduction to the Vacuum Degasser 8
System Overview 9
Leak and Waste Handling 9
This chapter gives an introduction to the module, instrument overview and internal connectors.
7Agilent Technologies
1 Introduction Introduction to the Vacuum Degasser
Introduction to the Vacuum Degasser
The Agilent 1260 Infinity High Performance Degasser, model G4225A, comprises four separate vacuum chambers with semipermeable tubings, a vacuum pump and control assembly. When the vacuum degasser is switched on, the control assembly turns on the vacuum pump, which generates a low pressure in the vacuum chambers. The pressure is measured by a pressure sensor. The vacuum degasser maintains the low pressure by a controlled leak in the air inlet filter and a regulation of the vacuum pump using the pressure sensor.
The LC pump draws the solvents from their bottles through the semipermeable tubes of the vacuum chambers. When solvents pass through the vacuum chambers any dissolved gas in the solvents permeates through the tubings into the vacuum chambers. The solvents will be degassed when leaving the outlets of the vacuum degasser.
Figure 1 Overview (only one of the four solvent channels is shown)
8 1260 Infinity High Performance Degasser - User Manual
Introduction 1 System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.
1260 Infinity High Performance Degasser - User Manual 9
1 Introduction System Overview
Figure 2 Leak and waste handling concept (overview - typical stack configuration as an example)
10 1260 Infinity High Performance Degasser - User Manual
Introduction 1 System Overview
The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2.5 L. For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).
The leak pan (2) (individually designed in each module) guides solvents to the front of the module. The concept covers also leakages on internal parts (e.g. the detectors flow cell). The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module to the next, as the solvent flows into the next modules leak funnel (3, B) and the connected corrugated waste tube (3, C). The corrugated waste tube guides the solvent to the next lower positioned modules leak tray and sensor.
The waste tube of the samplers needle wash port (4) guides solvents to waste.
The condense drain outlet of the autosampler cooler (5) guides condensate to waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom instruments (7) guides the solvent to a suitable waste container.
1260 Infinity High Performance Degasser - User Manual 11
1 Introduction System Overview
12 1260 Infinity High Performance Degasser - User Manual
1260 Infinity High Performance Degasser - User Manual
2 Site Requirements and Specifications
Site Requirements 14
Physical Specifications 17
Performance Specifications 18
This chapter provides information on environmental requirements, physical and performance specifications.
13Agilent Technologies
2 Site Requirements and Specifications Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 17. Consequently there is no voltage selector in the rear of the module.
WARNING Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
Connect your instrument to the specified line voltage only.
WARNING The module is partially energized when switched off, as long as the power cord is plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when the cover is opened and the module is connected to power.
Always unplug the power cable before opening the cover.
Do not connect the power cable to the instrument while the covers are removed.
CAUTION Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the power line at any time.
Make sure the power connector of the instrument can be easily reached and unplugged.
Provide sufficient space behind the power socket of the instrument to unplug the cable.
14 1260 Infinity High Performance Degasser - User Manual
Site Requirements and Specifications 2 Site Requirements
Power Cords
Different power cords are offered as options with the module. The female end of all power cords is identical. It plugs into the power- input socket at the rear. The male end of each power cord is different and designed to match the wall socket of a particular country or region.
WARNING Absence of ground connection or use of unspecified power cord
The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit.
Never operate your instrumentation from a power outlet that has no ground connection.
Never use a power cord other than the Agilent Technologies power cord designed for your region.
WARNING Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
WARNING Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment.
Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment.
1260 Infinity High Performance Degasser - User Manual 15
2 Site Requirements and Specifications Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 17) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
CAUTION Condensation within the module
Condensation will damage the system electronics.
Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module.
If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation.
16 1260 Infinity High Performance Degasser - User Manual
Site Requirements and Specifications 2 Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 5 kg (11 lbs)
Dimensions (height width depth)
80 x 345 435 mm (3.1 x 13.5 17 inches)
Line voltage 100 240 V~, 10 % Wide-ranging capability
Line frequency 50 or 60 Hz, 5 %
Power consumption 30 VA / 30 W / 102 BTU Maximum
Ambient operating temperature
0 55 C (32 131 F) 1
1 This temperature range represents the technical specifications for this instrument. The tempera- tures mentioned may not be suitable for all applications and all types of solvent.
Ambient non-operating temperature
-40 70 C (-40 158 F)
Humidity < 95 % r.h. at 40 C (104 F) Non-condensing
Operating altitude Up to 2000 m (6562 ft)
Non-operating altitude Up to 4600 m (15091 ft) For storing the module
Safety standards: IEC, CSA, UL
Installation category II, Pollution degree 2 For indoor use only.
1260 Infinity High Performance Degasser - User Manual 17
2 Site Requirements and Specifications Performance Specifications
Performance Specifications
Table 2 Performance Specifications Agilent 1260 Infinity High Performance Degasser
Type Specification
Number of solvent channels 4
Flow range 0 10 mL/min per channel
Internal volume per channel 0.45 mL per channel
Materials in contact with solvent TFE/PDD Copolymer, FEP, PEEK
pH range 1 14
18 1260 Infinity High Performance Degasser - User Manual
1260 Infinity High Performance Degasser - User Manual
3 Installing the Degasser
Unpacking the Vacuum Degasser 20
Optimizing the Stack Configuration 21
One Stack Configuration 22
Installation Information on Leak and Waste Handling 24
Installing the Vacuum Degasser 28
Flow Connections to the Vacuum Degasser 31
Operational Hints for the Vacuum Degasser 34
General Priming Instructions 34
Priming the Degasser with the Pump 35
Priming the Degasser with a Syringe (only recommended if priming with the pump fails) 36
Transporting the Vacuum Degasser 37
This chapter gives information about the preferred stack setup for your system and the installation of your module.
19Agilent Technologies
3 Installing the Degasser Unpacking the Vacuum Degasser
Unpacking the Vacuum Degasser
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment.
Delivery Checklist
Ensure all parts and materials have been delivered with your module. The delivery checklist is shown below. For parts identification please check the illustrated parts breakdown in Parts for Maintenance on page 65. Please report any missing or damaged parts to your local Agilent Technologies sales and service office.
Vacuum Degasser Delivery Checklist
CAUTION "Defective on arrival" problems
If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.
Notify your Agilent sales and service office about the damage.
An Agilent service representative will inspect the instrument at your site and initiate appropriate actions.
p/n Description
Vacuum Degasser
Power cord
G4800-64005 LC HW User Information + Utilities DVD
G1379-68705 Accessory Kit for 1260 Infinity High Performance Degasser
20 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Optimizing the Stack Configuration
Optimizing the Stack Configuration
If your module is part of a complete Agilent Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.
1260 Infinity High Performance Degasser - User Manual 21
3 Installing the Degasser Optimizing the Stack Configuration
One Stack Configuration
Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration (See Figure 3 on page 22 and Figure 4 on page 23). This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required.
Figure 3 Recommended Stack Configuration for 1260 Infinity (Front View)
22 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Optimizing the Stack Configuration
Figure 4 Recommended Stack Configuration for 1260 Infinity (Rear View)
1260 Infinity High Performance Degasser - User Manual 23
3 Installing the Degasser Installation Information on Leak and Waste Handling
Installation Information on Leak and Waste Handling
The Agilent 1200 Infinity Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.
WARNING Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
The volume of substances should be reduced to the minimum required for the analysis.
Never exceed the maximal permissible volume of solvents (6 L) in the solvent cabinet.
Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.
Arrange the bottles as specified in the usage guideline for the solvent cabinet.
A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet.
NOTE Recommendations for Solvent Cabinet
For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.
24 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Installation Information on Leak and Waste Handling
Figure 5 Leak and waste handling (overview - typical stack configuration as an example)
1260 Infinity High Performance Degasser - User Manual 25
3 Installing the Degasser Installation Information on Leak and Waste Handling
1 Stack the modules according to the adequate stack configuration.
The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module, see Figure 5 on page 25.
2 Connect data and power cables to the modules, see section Installing the Module below.
3 Connect capillaries and tubes to the modules, see section Flow Connections to the module below or the relevant system manual.
1 Solvent cabinet
2 Leak pan
3 Leak pan's outlet port (A), leak funnel (B) and corrugated waste tube (C)
4 Waste tube of the samplers needle wash
5 Condense drain outlet of the autosampler cooler
6 Waste tube of the purge valve
7 Waste tube
WARNING Toxic, flammable and hazardous solvents, samples and reagents
Keep solvent path free from blockages.
Keep the flow path closed (in case the pump in the system is equipped with a passive inlet valve, solvent may leak out due to hydrostatic pressure, even if your instrument is off).
Avoid loops.
Tubes must not sag.
Do not bend tubes.
Do not immerse tube end in waste liquid.
Do not intubate tubes in other tubes.
For correct tubing follow instructions on label attached to the module.
26 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Installation Information on Leak and Waste Handling
Figure 6 Warning label (illustration for correct waste tubing)
1260 Infinity High Performance Degasser - User Manual 27
3 Installing the Degasser Installing the Vacuum Degasser
Installing the Vacuum Degasser
Parts required Description
Power cord
Remote Cable, see Cable Overview on page 70
Hardware required Degasser
Preparations Locate bench space
Provide power connections
Unpack the vacuum degasser module
WARNING Abnormal conditions
In case of abnormal conditions during operation, the instrument must be disconnected from line.
To disconnect the instrument from line, unplug the power cord.
CAUTION "Defective on arrival" problems
If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.
Notify your Agilent sales and service office about the damage.
An Agilent service representative will inspect the instrument at your site and initiate appropriate actions.
28 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Installing the Vacuum Degasser
1 Place the vacuum degasser on the bench.
2 Ensure the power switch on the front of the vacuum degasser is OFF (switch stands out).
3 Connect the power cable to the power connector at the rear of the vacuum degasser.
4 Connect the interface cable to the vacuum degasser. The remote cable is a one way connection to send a not- ready signal from the degasser to the other modules or to shut down down the whole system after an error condition of the degasser.
Figure 7 Rear of the Vacuum Degasser
NOTE In an Agilent 1260 Infinity stack, the individual modules are connected through a CAN cable. The Agilent 1260 Infinity High Performance Degasser is an exception. The degasser can optionally be connected via the remote cable to the other modules of the stack. An Agilent 1260 Infinity Instant Pilot can be connected to the CAN bus at any of the modules in the system except for the degasser. The control software can be connected to the system through a LAN cable (via LAN-Card) to the detector or any other module except the degasser, if no detector is present.
1260 Infinity High Performance Degasser - User Manual 29
3 Installing the Degasser Installing the Vacuum Degasser
Figure 8 Front of the Vacuum Degasser
5 Press in the power switch to turn ON the vacuum degasser.
NOTE The power switch stays pressed in and a green indicator lamp in the power switch is ON when the vacuum degasser is turned ON. When the line power switch stands out and the green light is OFF, the vacuum degasser is turned OFF.
30 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Flow Connections to the Vacuum Degasser
Flow Connections to the Vacuum Degasser
1 Put the solvent cabinet with the bottle(s) on top of the vacuum degasser.
2 Remove the front cover by pressing the snap fasteners on both sides.
Figure 9 Removing the Front Cover
3 If the vacuum degasser is not used with an Agilent 1260 Infinity pump, connect the waste tube from the accessory kit to the waste outlet and place into your waste system.
4 Put the bottle head assemblies into solvent bottles containing your mobile phase.
Parts required Description
Solvent cabinet including solvent bottles (filled with solvent) and bottle head assemblies
Solvent outlet tubes
Syringe with adapter
Hardware required Degasser
Preparations Install the degasser
WARNING When opening capillary or tube fittings, solvents may leak out.
The handling of toxic and hazardous solvents and reagents can carry health risks.
Observe appropriate safety procedures (for example, wear goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used.
1260 Infinity High Performance Degasser - User Manual 31
3 Installing the Degasser Flow Connections to the Vacuum Degasser
5 Connect the solvent tubes from the bottle head assemblies to the inlet connectors A through D (typically the left connection of the channel) of the vacuum degasser. Use the mounting tool for simplified holding and fixing of the tubing fitting. Fix the tubes in the clips of the vacuum degasser.
6 Connect the outlet tubes to the output ports (typically right connection of the channel) of the vacuum degasser. Use the mounting tool to fix the tube screw.
7 Prime the degasser before first use (see Priming the Degasser with the Pump on page 35).
NOTE Atmospheric gases can diffuse through the tubing and dissolve in the mobile phase solvents. For best chromatographic results, keep the length of tubing between the vacuum degasser and your pump as short as possible.
32 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Flow Connections to the Vacuum Degasser
Figure 10 Flow Connection to the Vacuum Degasser
1260 Infinity High Performance Degasser - User Manual 33
3 Installing the Degasser Operational Hints for the Vacuum Degasser
Operational Hints for the Vacuum Degasser
General Priming Instructions
Before using a new degasser or new tubings for the first time:
1 Prime all tubings with at least 5 ml of iso- propanol no matter whether the channels will be used with organic mobile phase or with water.
If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows:
2 Replace the current solvent with iso- propanol, if current solvent is organic or with water, if current solvent is an inorganic buffer or contains salt.
WARNING When opening capillary or tube fittings, solvents may leak out.
The handling of toxic and hazardous solvents and reagents can carry health risks.
Observe appropriate safety procedures (for example, wear goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used.
34 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Operational Hints for the Vacuum Degasser
Priming the Degasser with the Pump
The vacuum degasser can be primed by drawing solvent through the degasser by pumping with the connected pump.
For priming the vacuum degasser using the pump the following is recommended:
1 Connect all tubings to and from degasser and pump.
2 Open the pumps purge valve to waste.
3 Pump at a flow rate of about 5 ml/min until the solvent reaches the pump. Pumping at higher flow rates will stress the system and might result in a failure to prime the degasser with the pump.
4 Prime each channel of the degasser and pump individually with at least 5 mL of solvent.
NOTE When the pump has been turned off for a certain time (for example, overnight) oxygen will rediffuse into the solvent channels between the degasser and the pump. Volatile fractions of a solvent mixture can evaporate from the solvent if left in the degasser with no flow for an extended time causing a composition change of the mixture. Therefore priming of the vacuum degasser and the pumping system is required before starting an application.
NOTE In case priming with the pump should fail, the degasser can be primed with a syringe. This might happen because the used solvent is highly volatile or the degasser and pump inlet lines are completely dry and the pump therefore fails to draw the solvent from the solvent bottle through the degasser channels into the pump.
1260 Infinity High Performance Degasser - User Manual 35
3 Installing the Degasser Operational Hints for the Vacuum Degasser
Priming the Degasser with a Syringe (only recommended if priming with the pump fails)
1 Disconnect solvent outlet tube of the channel that is supposed to be primed from your pump.
2 Connect syringe adapter to solvent outlet tube.
3 Push syringe adapter onto syringe.
4 Pull syringe plunger to draw at least 5 mL of solvent through degasser and tubing.
5 Replace the priming solvent with the new solvent of your choice.
6 Pull syringe plunger to draw at least 5 mL of solvent through degasser and tubing.
7 Disconnect syringe adapter from solvent tube.
8 Connect solvent tube to your pump.
9 Repeat step 1 on page 36 to step 8 on page 36 for the other solvent channels.
CAUTION Damage to degasser
Drawing the solvent through the degasser very quickly might destroy the chambers.
Draw the solvent through the degasser channels with moderate speed in order to avoid damage to the chambers!
NOTE When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes faster compared to priming with a pump. The solvent at the degasser outlet will therefore not be fully degassed. Pump for approximately 5 minutes with your selected flow rate before starting any application. This will allow the vacuum degasser to properly degas the solvent in the degasser tubes.
NOTE Priming the degasser with a syringe is only recommended if priming with a pump (see Priming the Degasser with the Pump on page 35) fails.
36 1260 Infinity High Performance Degasser - User Manual
Installing the Degasser 3 Transporting the Vacuum Degasser
Transporting the Vacuum Degasser
1 Pull the solvent inlet tubing out of the solvent bottle of channel A.
2 Let the pump draw solvent and air through channel A of the degasser, until the chamber of channel A is completely dry.
3 Repeat these steps for the remaining solvent channels.
WARNING Solvents leaking out
Solvents remaining in the solvent channels may leak out during transport. This can possibly cause personal damage.
Drain any remaining solvents from the degassing channels before transporting the degasser.
1260 Infinity High Performance Degasser - User Manual 37
3 Installing the Degasser Transporting the Vacuum Degasser
38 1260 Infinity High Performance Degasser - User Manual
1260 Infinity High Performance Degasser - User Manual
4 Using the Degasser
Leak and Waste Handling 40
When to Use a Vacuum Degasser 41
Prevent Blocking of Solvent Filters 42
Solvent Information 43
Material Information 43
This chapter explains how to use the module.
39Agilent Technologies
4 Using the Degasser Leak and Waste Handling
Leak and Waste Handling
For details on correct installation, see Installation Information on Leak and Waste Handling on page 24.
WARNING Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
The volume of substances should be reduced to the minimum required for the analysis.
Do not operate the instrument in an explosive atmosphere.
Never exceed the maximal permissible volume of solvents (6 L) in the solvent cabinet.
Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.
Arrange the bottles as specified in the usage guideline for the solvent cabinet.
A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet.
The residual free volume in the appropriate waste container must be large enough to collect the waste liquid.
Check the filling level of the waste container regularly.
To achieve maximal safety, check the correct installation regularly.
NOTE Recommendations for Solvent Cabinet
For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.
40 1260 Infinity High Performance Degasser - User Manual
Using the Degasser 4 When to Use a Vacuum Degasser
When to Use a Vacuum Degasser
For flow rates less than 10 mL/min:
if your detector is used with maximum sensitivity in the low UV wavelength range,
if your application requires optimum injection precision,
if your application requires highest retention time reproducibility (mandatory at flow rates below 0.5 mL/min,
if your sample or detection is sensitive to dissolved oxygen in the mobile phase (degradation),
with a fluorescence detector,
with an LC- MS- detector.
For capillary LC application with our Agilent 1260 Infinity Capillary and Nano LC System.
Generally a degasser should be used when negative effects due to dissolved gas in the mobile phase exceed the limits that are acceptable for the user. Negative effects that can be caused by dissolved gas are:
Unstable flow due to unstable pumping conditions. This may result in an increased pressure ripple or increased standard deviations of peak retention times and peak areas especially at low flow rates.
Baseline noise on detectors that are sensitive to changes in the refractive index,
sample degradation,
fluorescence quenching due to dissolved oxygen,
baseline drift in electrochemical detectors due to dissolved oxygen especially in reduction mode.
WARNING Unspecified Conditions
Operating the instrumentation under conditions other than its intended use might result in a potential safety hazard or might damage the instrumentation.
Never operate your instrumentation under conditions other than those specified by the vendor.
1260 Infinity High Performance Degasser - User Manual 41
4 Using the Degasser Prevent Blocking of Solvent Filters
Prevent Blocking of Solvent Filters
Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the pump. This is especially true for aqueous solvents or phosphate buffers (4 7 pH). The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the pump.
Use sterile, if possible amber solvent bottles to slow down algae growth.
Filter solvents through filters or membranes that remove algae.
Exchange solvents every two days or refilter.
If the application permits add 0.0001 0.001 M sodium azide to the solvent.
Place a layer of argon on top of your solvent.
Avoid exposure of the solvent bottles to direct sunlight.
Checking the Solvent Filters
The solvent filters are on the low- pressure side of the pumping system. A blocked filter therefore does not affect the pressure readings of the pump. The pressure readings cannot be used to identify blocked filters. If the solvent cabinet is placed on top of the vacuum degasser the filter condition can be checked in the following way:
Remove the tubing at the inlet port of the vacuum degasser. If the filter is in good condition the solvent will freely drip out of the solvent tube (due to hydrostatic pressure). If the solvent filter is partly blocked no solvent or only very little solvent will drip out of the solvent tube.
Cleaning the Solvent Filters
Remove the blocked solvent filter from the bottle- head assembly and place it in a beaker with concentrated nitric acid (35%) for one hour.
Thoroughly flush the filter with bidistilled water (remove all nitric acid).
Replace the filter.
NOTE Never use the system without solvent filter installed.
42 1260 Infinity High Performance Degasser - User Manual
Using the Degasser 4 Solvent Information
Solvent Information
Observe the following recommendations on the use of solvents.
Follow recommendations for avoiding the growth of algae, see pump manuals.
Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.4 m filters.
Avoid or minimize the use of solvents that may corrode parts in the flow path. Consider specifications for the pH range given for different materials like flow cells, valve materials etc. and recommendations in subsequent sections.
Material Information
Materials in Flow Path
Following materials are used in the flow path of this module:
Table 3 Materials in flow path
Part Materials
Internal tubings TFE/PDD copolymer, PFA
Inlets PEEK
Tubings FEP
Fittings ETFE
1260 Infinity High Performance Degasser - User Manual 43
4 Using the Degasser Solvent Information
Material Information
Materials in the flow path are carefully selected based on Agilents experiences in developing highest quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special conditions, please consult the material information section or contact Agilent.
Disclaimer
Subsequent data were collected from external resources and are meant as a reference. Agilent cannot guarantee the correctness and completeness of such information. Data is based on compatibility libraries, which are not specific for estimating the long- term life time under specific but highly variable conditions of UHPLC systems, solvents, solvent mixtures and samples. Information can also not be generalized due to catalytic effects of impurities like metal ions, complexing agents, oxygen etc. Apart from pure chemical corrosion, other effects like electro corrosion, electrostatic charging (especially for non- conductive organic solvents), swelling of polymer parts etc. need to be considered. Most data available refers to room temperature (typically 20 25 C, 68 77 F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials.
PEEK
PEEK (Polyether- Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is therefore the material of choice for UHPLC and biochemical instrumentation.
It is stable in a wide pH range, and inert to many common solvents.
There is still a number of known incompatibilities with chemicals such as chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %, sulphuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogenes or aequous halogene solutions, phenol and derivatives (cresols, salicylic acid etc.).
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Using the Degasser 4 Solvent Information
Polyimide
Agilent uses semi- crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent.
Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like ammonia (e.g. ammonium salts in basic conditions) or acetates.
Polyethylene (PE)
Agilent uses UHMW (ultra- high molecular weight)- PE/PTFE blends for yellow piston and wash seals, which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps.
Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12.5. It is compatible to many organic solvents used in chromatographic systems like methanol, acetonitrile and isopropanol. It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar.
Tantalum (Ta)
Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides.
1260 Infinity High Performance Degasser - User Manual 45
4 Using the Degasser Solvent Information
Stainless Steel (ST)
Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by acids below pH 2.3. It can also corrode in following solvents:
Solutions of alkali halides, their respective acids (for example, lithium iodide, potassium chloride, and so on) and aqueous solutions of halogens.
High concentrations of inorganic acids like nitric acid, sulfuric acid and organic solvents especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel).
Halogenated solvents or mixtures which form radicals and/or acids, for example:
2 CHCl3 + O2 2 COCl2 + 2 HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.
Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di- isopropylether). Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides.
Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel.
Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra- acetic acid).
Mixtures of carbon tetrachloride with 2- propanol or THF.
Diamond-Like Carbon (DLC)
Diamond- Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
Fused silica and Quartz (SiO2)
Fused silica is used in 1290 Infinity Flow Cells and capillaries. Quartz is used for classical flow cell windows. It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides. It is corroded by strong bases and should not be used above pH 12 at room temperature. The corrosion of flow cell windows can negatively affect measurement results. For a pH greater than 12, the use of flow cells with sapphire windows is recommended.
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Using the Degasser 4 Solvent Information
Gold
Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia.
Zirconium Oxide (ZrO2)
Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
Platinum/Iridium
Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
Fluorinated polymers (PTFE, PFA, FEP, FFKM)
Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons.
TFE/PDD copolymer tubings, which are used in all Agilent degassers except G1322A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel. They have limited life time in the presence of Hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let dry out the chamber. For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off.
Sapphire, Ruby and Al2O3-based ceramics
Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
1260 Infinity High Performance Degasser - User Manual 47
4 Using the Degasser Solvent Information
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1260 Infinity High Performance Degasser - User Manual
5 Optimizing Performance
Increasing the Degasser Performance and Degassing Level 50
This chapter gives hints on how to optimize the performance or use additional devices.
49Agilent Technologies
5 Optimizing Performance Increasing the Degasser Performance and Degassing Level
Increasing the Degasser Performance and Degassing Level
For some critical applications it might be useful to increase the degassers performance and degassing level by using two channels of the degasser in series with only one solvent channel of the pump. For doing so:
1 Connect the solvent inlet tubing (bottle head assembly, Bottle- head assembly (G1311- 60003)) coming from the solvent bottle to the inlet line of the first channel, you want to use.
2 Connect the outlet of the first channel to the inlet if the 2nd solvent channel of the degasser with the help of the short connecting tubing (p/n G1379- 68706), delivered with the accessory kit of the degasser.
3 Connect to the outlet of the 2nd channel of the degasser and into the pump.
Figure 11 Connecting two degasser channels in series
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1260 Infinity High Performance Degasser - User Manual
6 Troubleshooting and Diagnostics
Overview of the Degasser's Indicators 52
Status Indicators 53
Module Status Indicator 54
Hardware Symptoms 55
All Lamps are Off 55
If the Status Indicator is Red 56
If the Status Indicator is Yellow and the Vacuum Pump is not Running 56
Status Indicator becomes Red and Vacuum Pump was Running 56
This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.
51Agilent Technologies
6 Troubleshooting and Diagnostics Overview of the Degasser's Indicators
Overview of the Degasser's Indicators
Status Indicators
The vacuum degasser is provided with two status indicators which indicate the operational state (ready, busy, and error states) of the vacuum degasser. The status indicators provide a quick visual check of the operation of the vacuum degasser (see Figure 12 on page 53).
Hardware Symptoms
A red status lamp at the vacuum degasser indicates a problem with the vacuum system or with the electronic control. The vacuum degasser generates an error output on the remote lines. The following pages describe hardware symptoms which help you to isolate the cause of a hardware failure (see Hardware Symptoms on page 55).
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Troubleshooting and Diagnostics 6 Status Indicators
Status Indicators
Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status.
Figure 12 Location of Status Indicators
1260 Infinity High Performance Degasser - User Manual 53
6 Troubleshooting and Diagnostics Module Status Indicator
Module Status Indicator
The module status indicator indicates one of six possible module conditions:
When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis.
A green status indicator, indicates the module is performing an analysis (run mode).
A yellow indicator indicates a not- ready condition. The module is in a not- ready state when it is waiting for a specific condition to be reached or completed (for example, immediately after changing a set point), or while a self- test procedure is running.
An error condition is indicated when the status indicator is red. An error condition indicates the module has detected an internal problem which affects correct operation of the module. Usually, an error condition requires attention (e.g. leak, defective internal components). An error condition always interrupts the analysis.
If the error occurs during analysis, it is propagated within the LC system, i.e. a red LED may indicate a problem of a different module. Use the status display of your user interface for finding the root cause/module of the error.
A blinking indicator indicates that the module is in resident mode (e.g. during update of main firmware).
A fast blinking indicator indicates that the module is in boot loader mode (e.g. during update of main firmware). In such a case try to re- boot the module or try a cold- start.
CAUTION Red status LED indicates error
This indicates either an internal leak in the vacuum system or an electronic failure.
To prevent any damage, switch off the vacuum degasser and remove the solvent bottles from the solvent cabinet to stop any gravity-caused flow of solvent into the vacuum chamber.
In case of an internal leak it is possible that solvent may enter the vacuum chamber and solvent may leak into the waste drain.
Apply troubleshhoting information in If the Status Indicator is Red on page 56.
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Troubleshooting and Diagnostics 6 Hardware Symptoms
Hardware Symptoms
In case of a problem with the vacuum system or the electronic control the vacuum degasser status lamp will be red. The vacuum degasser will generate an error output on the remote lines. This will shut down other system modules when connected via remote cable, see Installing the Vacuum Degasser on page 28. The vacuum degasser itself is not able to generate any error messages in the Agilent 1260 Infinity system logbook. The following pages describe hardware symptoms which help you to isolate the cause of a hardware failure.
All Lamps are Off
If all other modules in the system are on (power switch lamp is green) and are recognized by the connected user interface (module parameters can be set, module- specific screens appear, and so on), then do the following to determine the problem with the vacuum degasser:
Ensure the power cable is connected to the degasser, and the power cable is connected to line power.
Ensure the power switch on the front of the module is ON.
Ensure the power fuses are OK.
The fuse holders are located on the rear panel of the vacuum degasser and are part of the power socket. Check the fuses (see Exchanging the Fuses of the Power Inline Filter on page 63), and change if necessary:
If the previous steps did not solve the problem, contact your Agilent service representative.
1260 Infinity High Performance Degasser - User Manual 55
6 Troubleshooting and Diagnostics Hardware Symptoms
If the Status Indicator is Red
Sufficient vacuum is normally built up after the initial start- up and controlled by the pressure sensor.
If the vacuum cannot be reached, the vacuum degasser will switch to the error state. The error condition can be reset by turning the vacuum degasser off and on again.
If the Status Indicator is Yellow and the Vacuum Pump is not Running
Please contact your Agilent service representative.
Status Indicator becomes Red and Vacuum Pump was Running
Sufficient vacuum is normally built up after the initial start- up and controlled by the pressure sensor.
If the vacuum cannot be reached, the vacuum degasser will switch to the error state. The error condition can be reset by turning the vacuum degasser off and on again.
The following parts can be responsible for an insufficient vacuum:
1 Leaky tubing,
2 leaky or defective internal parts.
Contact your Agilent service representative.
NOTE The status indicator is yellow during the startup phase of the degasser until the operating pressure is reached. This may take several minutes.
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1260 Infinity High Performance Degasser - User Manual
7 Maintenance
Warnings and Cautions 58
Introduction to Maintenance 60
Cleaning the Module 61
Assembling the Main Cover 62
Exchanging the Fuses of the Power Inline Filter 63
This chapter describes the maintenance of the module.
57Agilent Technologies
7 Maintenance Warnings and Cautions
Warnings and Cautions
WARNING Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
The volume of substances should be reduced to the minimum required for the analysis.
Do not operate the instrument in an explosive atmosphere.
WARNING Electrical shock
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened.
Do not remove the cover of the module.
Only certified persons are authorized to carry out repairs inside the module.
WARNING Personal injury or damage to the product
Agilent is not responsible for any damages caused, in whole or in part, by improper use of the products, unauthorized alterations, adjustments or modifications to the products, failure to comply with procedures in Agilent product user guides, or use of the products in violation of applicable laws, rules or regulations.
Use your Agilent products only in the manner described in the Agilent product user guides.
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Maintenance 7 Warnings and Cautions
WARNING Sharp metal edges
Sharp-edged parts of the equipment may cause injuries.
To prevent personal injury, be careful when getting in contact with sharp metal areas.
CAUTION Safety standards for external equipment
If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.
1260 Infinity High Performance Degasser - User Manual 59
7 Maintenance Introduction to Maintenance
Introduction to Maintenance
The vacuum degasser is designed for easy repair. The most frequent repairs such as exchanging power fuses and assembling the main cover can be performed by the user.
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Maintenance 7 Cleaning the Module
Cleaning the Module
To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent.
WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module
Do not use an excessively damp cloth during cleaning.
Drain all solvent lines before opening any connections in the flow path.
1260 Infinity High Performance Degasser - User Manual 61
7 Maintenance Assembling the Main Cover
Assembling the Main Cover
When If cover is broken
Tools required Description
None
Parts required # p/n Description
1 5065-9989 Cover kit (includes base, top, left and right)
CAUTION Wrong assembly
Once installed wrongly, you may not be able to remove the side from the top part.
Make sure to install the side parts in the correct orientation.
1 Place the top part on the bench and insert the left and right side into the top part.
2 Replace the cover.
Next Steps:
3 Replace the vacuum degasser in the stack and reconnect the cables and capillaries.
4 Turn on the vacuum degasser.
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Maintenance 7 Exchanging the Fuses of the Power Inline Filter
Exchanging the Fuses of the Power Inline Filter
When When defective
Tools required Description
None
Parts required # p/n Description
2 2110-0458 Fuse: 250V, T 500 mA, compatible to all supported line voltages
NOTE The module power supply has wide-ranging capability (see Table 1 on page 17). It accepts any line voltage in the range mentioned in the table. Consequently there is no voltage selector in the rear of the module. There are two externally accessible fuses, that protect the power supply. These fuses are identical for all accepted line voltages.
WARNING Potential hazard of physical injury or death or damage to the instrument.
Using fuses other than the one specified for this module and purpose can lead to personal injury or damage of the electronic components.
Never use fuses other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
WARNING Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
1260 Infinity High Performance Degasser - User Manual 63
7 Maintenance Exchanging the Fuses of the Power Inline Filter
1 Switch off the power switch at the front of the instrument.
2 Remove the power cable from the power connector at the rear of the instrument.
3 Press down the clip of the fuse holder and pull out of the power socket.
4 Remove the fuses from the fuse holder.
5 Ensure the fuse wires inside the fuses are not broken. If a test meter is available, check the resistance of each fuse. A good fuse shows a low resistance (typically less than 1 Ohm).
6 If a fuse is defective (wire broken or high resistance), insert a new fuse.
7 Reinsert the fuse holder and the power cable.
8 Switch on the power switch.
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1260 Infinity High Performance Degasser - User Manual
8 Parts for Maintenance
Cover Parts 66
Fuse 67
Accessory Kit 68
This chapter provides information on parts for maintenance.
65Agilent Technologies
8 Parts for Maintenance Cover Parts
Cover Parts
Figure 13 Cover Parts
Item p/n Description
1 5065-9989 Cover kit (includes base, top, left and right)
2 5043-0207 Name plate 1260
3 5065-9990 Front cover
4 5041-8365 Blank plug for unused channels
5 5041-8387 Tube clip
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Parts for Maintenance 8 Fuse
Fuse
Figure 14 Fuse
Item p/n Description
1 2110-0458 Fuse: 250V, T 500 mA, compatible to all supported line voltages
1260 Infinity High Performance Degasser - User Manual 67
8 Parts for Maintenance Accessory Kit
Accessory Kit
Item p/n Description
1 G1379-68705 Accessory Kit for 1260 Infinity High Performance Degasser
2 G1379-68706 Connecting tubing (to connect to channels in series for increased performance) 2x
3 G1322-67300 Kit of 4 solvent tubes including labels for connection degasser to MCGV (Quaternary Pump)
4 5062-2461 Waste tube, 5 m (reorder pack)
5 0100-1710 Mounting Tool for Tubing Connections
6 5061-3378 Remote Cable
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1260 Infinity High Performance Degasser - User Manual
9 Identifying Cables
Cable Overview 70
Analog Cables 72
Remote Cables 74
BCD Cables 77
CAN Cable 79
External Contact Cable 80
Agilent Module to PC 81
Agilent 1200 Module to Printer 82
This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.
69Agilent Technologies
9 Identifying Cables Cable Overview
Cable Overview
Analog cables
Remote cables
BCD cables
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
p/n Description
35900-60750 Agilent module to 3394/6 integrators
35900-60750 Agilent 35900A A/D converter
01046-60105 Analog cable (BNC to general purpose, spade lugs)
p/n Description
03394-60600 Agilent module to 3396A Series I integrators
3396 Series II / 3395A integrator, see details in section Remote Cables on page 74
03396-61010 Agilent module to 3396 Series III / 3395B integrators
5061-3378 Remote Cable
01046-60201 Agilent module to general purpose
p/n Description
03396-60560 Agilent module to 3396 integrators
G1351-81600 Agilent module to general purpose
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Identifying Cables 9 Cable Overview
CAN cables
LAN cables
External Contact Cable
RS-232 cables
p/n Description
5181-1516 CAN cable, Agilent module to module, 0.5 m
5181-1519 CAN cable, Agilent module to module, 1 m
p/n Description
5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)
5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection)
p/n Description
G1103-61611 General Purpose Cable
p/n Description
G1530-60600 RS-232 cable, 2 m
RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
5181-1561 RS-232 cable, 8 m
1260 Infinity High Performance Degasser - User Manual 71
9 Identifying Cables Analog Cables
Analog Cables
One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made.
Agilent Module to 3394/6 Integrators
Agilent Module to BNC Connector
p/n 35900-60750 Pin 3394/6 Pin Agilent module
Signal Name
1 Not connected
2 Shield Analog -
3 Center Analog +
p/n 8120-1840 Pin BNC Pin Agilent module
Signal Name
Shield Shield Analog -
Center Center Analog +
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Identifying Cables 9 Analog Cables
Agilent Module to General Purpose
p/n 01046-60105 Pin Pin Agilent module
Signal Name
1 Not connected
2 Black Analog -
3 Red Analog +
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9 Identifying Cables Remote Cables
Remote Cables
One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.
Agilent Module to 3396A Integrators
Agilent Module to 3396 Series II / 3395A Integrators
Use the cable Agilent module to 3396A Series I integrators (03394- 60600) and cut pin #5 on the integrator side. Otherwise the integrator prints START; not ready.
p/n 03394-60600 Pin 3396A Pin Agilent module
Signal Name Active (TTL)
9 1 - White Digital ground
NC 2 - Brown Prepare run Low
3 3 - Gray Start Low
NC 4 - Blue Shut down Low
NC 5 - Pink Not connected
NC 6 - Yellow Power on High
5,14 7 - Red Ready High
1 8 - Green Stop Low
NC 9 - Black Start request Low
13, 15 Not connected
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Identifying Cables 9 Remote Cables
Agilent Module to 3396 Series III / 3395B Integrators
Agilent Module to Agilent 35900 A/D Converters
p/n 03396-61010 Pin 33XX Pin Agilent module
Signal Name Active (TTL)
9 1 - White Digital ground
NC 2 - Brown Prepare run Low
3 3 - Gray Start Low
NC 4 - Blue Shut down Low
NC 5 - Pink Not connected
NC 6 - Yellow Power on High
14 7 - Red Ready High
4 8 - Green Stop Low
NC 9 - Black Start request Low
13, 15 Not connected
p/n 5061-3378 Pin 35900 A/D
Pin Agilent module
Signal Name Active (TTL)
1 - White 1 - White Digital ground
2 - Brown 2 - Brown Prepare run Low
3 - Gray 3 - Gray Start Low
4 - Blue 4 - Blue Shut down Low
5 - Pink 5 - Pink Not connected
6 - Yellow 6 - Yellow Power on High
7 - Red 7 - Red Ready High
8 - Green 8 - Green Stop Low
9 - Black 9 - Black Start request Low
1260 Infinity High Performance Degasser - User Manual 75
9 Identifying Cables Remote Cables
Agilent Module to General Purpose
p/n 01046-60201 Wire Color Pin Agilent module
Signal Name Active (TTL)
White 1 Digital ground
Brown 2 Prepare run Low
Gray 3 Start Low
Blue 4 Shut down Low
Pink 5 Not connected
Yellow 6 Power on High
Red 7 Ready High
Green 8 Stop Low
Black 9 Start request Low
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Identifying Cables 9 BCD Cables
BCD Cables
One end of these cables provides a 15- pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to
Agilent Module to General Purpose
p/n G1351-81600 Wire Color Pin Agilent module
Signal Name BCD Digit
Green 1 BCD 5 20
Violet 2 BCD 7 80
Blue 3 BCD 6 40
Yellow 4 BCD 4 10
Black 5 BCD 0 1
Orange 6 BCD 3 8
Red 7 BCD 2 4
Brown 8 BCD 1 2
Gray 9 Digital ground Gray
Gray/pink 10 BCD 11 800
Red/blue 11 BCD 10 400
White/green 12 BCD 9 200
Brown/green 13 BCD 8 100
not connected 14
not connected 15 + 5 V Low
1260 Infinity High Performance Degasser - User Manual 77
9 Identifying Cables BCD Cables
Agilent Module to 3396 Integrators
p/n 03396-60560 Pin 3396 Pin Agilent module
Signal Name BCD Digit
1 1 BCD 5 20
2 2 BCD 7 80
3 3 BCD 6 40
4 4 BCD 4 10
5 5 BCD0 1
6 6 BCD 3 8
7 7 BCD 2 4
8 8 BCD 1 2
9 9 Digital ground
NC 15 + 5 V Low
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Identifying Cables 9 CAN Cable
CAN Cable
Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors.
CAN Cables
LAN Cables
p/n Description
5181-1516 CAN cable, Agilent module to module, 0.5 m
5181-1519 CAN cable, Agilent module to module, 1 m
p/n Description
5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)
5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection)
1260 Infinity High Performance Degasser - User Manual 79
9 Identifying Cables External Contact Cable
External Contact Cable
One end of this cable provides a 15- pin plug to be connected to Agilent modules interface board. The other end is for general purpose.
Agilent Module Interface Board to general purposes
p/n G1103-61611 Color Pin Agilent module
Signal Name
White 1 EXT 1
Brown 2 EXT 1
Green 3 EXT 2
Yellow 4 EXT 2
Grey 5 EXT 3
Pink 6 EXT 3
Blue 7 EXT 4
Red 8 EXT 4
Black 9 Not connected
Violet 10 Not connected
Grey/pink 11 Not connected
Red/blue 12 Not connected
White/green 13 Not connected
Brown/green 14 Not connected
White/yellow 15 Not connected
5 10 15
1
11 6
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Identifying Cables 9 Agilent Module to PC
Agilent Module to PC
p/n Description
G1530-60600 RS-232 cable, 2 m
RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
5181-1561 RS-232 cable, 8 m
1260 Infinity High Performance Degasser - User Manual 81
9 Identifying Cables Agilent 1200 Module to Printer
Agilent 1200 Module to Printer
p/n Description
5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module.
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1260 Infinity High Performance Degasser - User Manual
10 Hardware Information
Operating Principle of the 1260 Infinity High Performance Degasser 84
Electrical Connections 85
Rear view of the module 85
Interfaces 86
Overview Interfaces 89
This chapter describes the degasser in more detail on hardware and electronics.
83Agilent Technologies
10 Hardware Information Operating Principle of the 1260 Infinity High Performance Degasser
Operating Principle of the 1260 Infinity High Performance Degasser
During initialization, the status indicator is amber and the degasser vacuum pump tries to reach a target pressure below 146 mbar/hPa. If this limit cannot be reached within 10 minutes, an error is thrown and the status indicator gets red. As soon as the limit has been reached, which typically happens after about 2 minutes, the pump goes to a continuous regulation mode, where the pump tries to regulate to 100 mbar/hPa target pressure. If this pressure limit cannot be reached, it is increased stepwise to a maximum of 146 mbar and the status indicator goes to amber. If a stable pressure between 100 mbar and 146 mbar max. can be kept, the status indicator switches to off. If the maximum limit of 146 mbar is exceeded, the status indicator goes to red and an error is thrown.
84 1260 Infinity High Performance Degasser - User Manual
Hardware Information 10 Electrical Connections
Electrical Connections
The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shut down, prepare, and so on.
With the appropriate software, the RS- 232C connector may be used to control the module from a computer through a RS- 232C connection. This connector is activated and can be configured with the configuration switch.
The power input socket accepts a line voltage of 100 240 VAC 10 % with a line frequency of 50 or 60 Hz. Maximum power consumption varies by module. There is no voltage selector on your module because the power supply has wide- ranging capability. There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.
Rear view of the module
Figure 15 Rear View of Degasser Electrical Connections and Label
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
1260 Infinity High Performance Degasser - User Manual 85
10 Hardware Information Interfaces
Interfaces
The Agilent 1200 Infinity Series modules provide the following interfaces:
Table 4 Agilent 1200 Infinity Series Interfaces
Module CAN LAN/BCD (optional)
LAN (on-board)
RS-232 Analog APG Remote
Special
Pumps
G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed. G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio-inert Quat Pump
2 Yes No Yes 1 Yes
G4220A/B Bin Pump G4204A Quat Pump
2 No Yes Yes No Yes CAN-DC- OUT for CAN slaves
G1361A Prep Pump 2 Yes No Yes No Yes CAN-DC- OUT for CAN slaves
Samplers
G1329B ALS G2260A Prep ALS
2 Yes No Yes No Yes THERMOSTAT for G1330B/K1330B
G1364B FC-PS G1364C FC-AS G1364D FC-S G1367E HiP ALS K1367E HiP ALS Clinical Ed. G1377A HiP micro ALS G2258A DL ALS G5664A Bio-inert FC-AS G5667A Bio-inert Autosampler
2 Yes No Yes No Yes THERMOSTAT for G1330B/K1330B CAN-DC- OUT for CAN slaves
G4226A ALS 2 Yes No Yes No Yes
86 1260 Infinity High Performance Degasser - User Manual
Hardware Information 10 Interfaces
Detectors
G1314B VWD VL G1314C VWD VL+
2 Yes No Yes 1 Yes
G1314E/F VWD K1314F Clinical Ed.
2 No Yes Yes 1 Yes
G4212A/B DAD K4212B DAD Clinical Ed.
2 No Yes Yes 1 Yes
G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL
2 No Yes Yes 2 Yes
G1321B FLD K1321B FLD Clinical Ed. G1321C FLD
2 Yes No Yes 2 Yes
G1362A RID 2 Yes No Yes 1 Yes
G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO
Others
G1170A Valve Drive 2 No No No No No 1
G1316A/C TCC K1316C TCC Clinical Ed.
2 No No Yes No Yes
G1322A DEG K1322A DEG Clinical Ed.
No No No No No Yes AUX
G1379B DEG No No No Yes No Yes
G4225A DEG K4225A DEG Clinical Ed.
No No No Yes No Yes
Table 4 Agilent 1200 Infinity Series Interfaces
Module CAN LAN/BCD (optional)
LAN (on-board)
RS-232 Analog APG Remote
Special
1260 Infinity High Performance Degasser - User Manual 87
10 Hardware Information Interfaces
CAN connectors as interface to other modules
LAN connector as interface to the control software
RS- 232C as interface to a computer
REMOTE connector as interface to other Agilent products
Analog output connector(s) for signal output
G4227A Flex Cube 2 No No No No No CAN-DC- OUT for CAN slaves 1
G4240A CHIP CUBE 2 Yes No Yes No Yes CAN-DC- OUT for CAN slaves THERMOSTAT for G1330A/B (NOT USED), K1330B
1 Requires a HOST module with on-board LAN (e.g. G4212A or G4220A with minimum firmware B.06.40 or C.06.40) or with ad- ditional G1369C LAN Card
Table 4 Agilent 1200 Infinity Series Interfaces
Module CAN LAN/BCD (optional)
LAN (on-board)
RS-232 Analog APG Remote
Special
NOTE The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via LAN. The inter-module communication is done via CAN.
88 1260 Infinity High Performance Degasser - User Manual
Hardware Information 10 Interfaces
Overview Interfaces
CAN
The CAN is inter- module communication interface. It is a 2- wire serial bus system supporting high speed data communication and real- time requirement.
LAN
The modules have either an interface slot for an LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on- board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD). This interface allows the control of the module/system via a PC with the appropriate control software. Some modules have neither on- board LAN nor an interface slot for a LAN card (e.g. G1170A Valve Drive or G4227A Flex Cube). These are hosted modules and require a Host module with firmware B.06.40 or later or with additional G1369C LAN Card.
RS-232C (Serial)
The RS- 232C connector is used to control the module from a computer through RS- 232C connection, using the appropriate software. This connector can be configured with the configuration switch module at the rear of the module. Refer to Communication Settings for RS- 232C.
NOTE If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN should be connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent detector is part of the system, the LAN interface should be installed in the pump or autosampler.
NOTE There is no configuration possible on main boards with on-board LAN. These are pre-configured for
19200 baud,
8 data bit with no parity and
one start bit and one stop bit are always used (not selectable).
1260 Infinity High Performance Degasser - User Manual 89
10 Hardware Information Interfaces
The RS- 232C is designed as DCE (data communication equipment) with a 9- pin male SUB- D type connector. The pins are defined as:
Figure 16 RS-232 Cable
Analog Signal Output
The analog signal output can be distributed to a recording device. For details refer to the description of the modules main board.
Table 5 RS-232C Connection Table
Pin Direction Function
1 In DCD
2 In RxD
3 Out TxD
4 Out DTR
5 Ground
6 In DSR
7 Out RTS
8 In CTS
9 In RI
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Hardware Information 10 Interfaces
APG Remote
The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on.
Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique).
To provide maximum safety within a distributed analysis system, one line is dedicated to SHUT DOWN the systems critical parts in case any module detects a serious problem. To detect whether all participating modules are switched on or properly powered, one line is defined to summarize the POWER ON state of all connected modules. Control of analysis is maintained by signal readiness READY for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal levels are defined as:
standard TTL levels (0 V is logic true, + 5.0 V is false),
fan- out is 10,
input load is 2.2 kOhm against + 5.0 V, and
output are open collector type, inputs/outputs (wired- or technique).
NOTE All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with respect to the ground terminal).
1260 Infinity High Performance Degasser - User Manual 91
10 Hardware Information Interfaces
Special Interfaces
There is no special interface for this module.
Table 6 Remote Signal Distribution
Pin Signal Description
1 DGND Digital ground
2 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities.
3 START (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities.
4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump). Receiver is any module capable to reduce safety risk.
5 Not used
6 POWER ON (H) All modules connected to system are switched on. Receiver is any module relying on operation of others.
7 READY (H) System is ready for next analysis. Receiver is any sequence controller.
8 STOP (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection). Receiver is any module performing run-time controlled activities.
9 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler.
92 1260 Infinity High Performance Degasser - User Manual
1260 Infinity High Performance Degasser - User Manual
11 Appendix
General Safety Information 94
The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 97
Radio Interference 98
Sound Emission 99
Agilent Technologies on Internet 100
This chapter provides addition information on safety, legal and web.
93Agilent Technologies
11 Appendix General Safety Information
General Safety Information
General Safety Information
The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.
Safety Standards
This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards.
WARNING Ensure the proper usage of the equipment.
The protection provided by the equipment may be impaired.
The operator of this instrument is advised to use the equipment in a manner as specified in this manual.
94 1260 Infinity High Performance Degasser - User Manual
Appendix 11 General Safety Information
Operation
Before applying power, comply with the installation section. Additionally the following must be observed.
Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto- transformers, and devices connected to it must be connected to a protective earth via a ground socket. Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury. Whenever it is likely that the protection has been impaired, the instrument must be made inoperative and be secured against any intended operation.
Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short- circuiting of fuse holders must be avoided.
Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed. Energy available at many points may, if contacted, result in personal injury.
Any adjustment, maintenance, and repair of the opened instrument under voltage should be avoided whenever possible. When inevitable, this has to be carried out by a skilled person who is aware of the hazard involved. Do not attempt internal service or adjustment unless another person, capable of rendering first aid and resuscitation, is present. Do not replace components with power cable connected.
Do not operate the instrument in the presence of flammable gases or fumes. Operation of any electrical instrument in such an environment constitutes a definite safety hazard.
Do not install substitute parts or make any unauthorized modification to the instrument.
Capacitors inside the instrument may still be charged, even though the instrument has been disconnected from its source of supply. Dangerous voltages, capable of causing serious personal injury, are present in this instrument. Use extreme caution when handling, testing and adjusting.
When working with solvents, observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used.
1260 Infinity High Performance Degasser - User Manual 95
11 Appendix General Safety Information
Safety Symbols
Table 7 Safety Symbols
Symbol Description
The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product.
The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up.
WARNING A WARNING
alerts you to situations that could cause physical injury or death.
Do not proceed beyond a warning until you have fully understood and met the indicated conditions.
CAUTION A CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
Do not proceed beyond a caution until you have fully understood and met the indicated conditions.
96 1260 Infinity High Performance Degasser - User Manual
Appendix 11 The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC)
The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC)
Abstract
The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005.
NOTE This product complies with the WEEE Directive (2002/96/EC) marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste.
Product Category:
With reference to the equipment types in the WEEE Directive Annex I, this product is classed as a Monitoring and Control Instrumentation product.
NOTE Do not dispose off in domestic household waste
To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information.
1260 Infinity High Performance Degasser - User Manual 97
11 Appendix Radio Interference
Radio Interference
Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations.
Test and Measurement
If test and measurement equipment is operated with unscreened cables, or used for measurements on open set- ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
98 1260 Infinity High Performance Degasser - User Manual
Appendix 11 Sound Emission
Sound Emission
Manufacturers Declaration
This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
Sound Pressure Lp < 70 dB (A)
At Operator Position
Normal Operation
According to ISO 7779:1988/EN 27779/1991 (Type Test)
1260 Infinity High Performance Degasser - User Manual 99
11 Appendix Agilent Technologies on Internet
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web site on the Internet at:
http://www.agilent.com
100 1260 Infinity High Performance Degasser - User Manual
Index
Index
A accessory kit 68
Agilent on internet 100
algae growth 42
ambient non-operating temperature 17
ambient operating temperature 17
analog signal 90
analog cable 72
APG remote connector 29
apg remote 91
B BCD
cable 77
bench space 16
C cable
analog 72 BCD 77 CAN 79, 29 external contact 80 LAN 79 power 55 remote 74, 55 RS-232 81
cables analog 70 BCD 70 CAN 71 external contact 71 LAN 71
overview 70 remote 70 RS-232 71
CAN cable 29
CAN cable 79
changing solvents 34, 36
cleaning 61
condensation 16
configuration one stack 22, 22
control assembly 8
cover 66
D defect on arrival 20
delivery checklist 20
dimensions 17
E electrical connections
descriptions of 85
electronic waste 97
error state 56, 56
exchanging solenoid valve 63
external contact cable 80
F flow connections 31, 31
flow rate 18
frequency range 17
fuse 55, 63
H hardware symptoms 55
highest injection precision 41
highest retention time reproducibility 41
humidity 17
I information
on sound emission 99
installation bench space 16
interfaces 86
internet 100
introduction to the degasser 8
L lamps
off, degasser appears dead 55 status indicator is red 56, 56 status indicator is yellow, vacuum pump not running 56
LAN cable 79
line frequency 17
line voltage 17
M main cover, assembling 62
materials in contact with solvent 18
maximum flow rate 18
maximum sensitivity 41
1260 Infinity High Performance Degasser - User Manual 101
Index
N non-operating altitude 17
non-operating temperature 17
number of channels 18
O operating Altitude 17
operating temperature 17
optimization stack configuration 21
P packaging
damaged 20
parts accessory kit 68 cover 66 damaged 20 missing 20
performance specifications 18
pH range 18
physical specifications 17
power cable 55
power consideration 14
power consumption 17
power cords 15
power supply 63
power switch 29
pressure sensor 8
priming with a pump 35 with a syringe 35
principle of operation 8
R radio interference 98
remote cable 55
remote
cable 74
RS-232C cable 81
S safety class I 94
safety general information 94 standards 17 symbols 96
site requirements power cords 15
solenoid valve 63
solvent filters checking 42 cleaning 42 prevent blocking 42
sound emission 99
special interfaces 92
specification performance specifications 18 physical 17
status indicator 54, 56, 56, 56
syringe adapter 36
system setup and installation optimizing stack configuration 21
T transportation 37
troubleshooting status indicators 53
V vacuum pump 8
voltage range 17
voltage selector 63
volume per channel 18
W waste
electrical and electronic equipment 97
WEEE directive 97
weight 17
102 1260 Infinity High Performance Degasser - User Manual
Index
1260 Infinity High Performance Degasser - User Manual 103
www.agilent.com
In This Book
This manual contains information about the Agilent 1260 Infinity High Performance Degasser (G4225A). The manual describes the following:
introduction,
site requirements and specifications,
installing the degasser,
using the degasser,
troubleshooting and diagnostics,
maintenance,
parts for maintenance,
identifying cables,
appendix.
Agilent Technologies 2011-2013, 2014
Printed in Germany 02/2014
*G4225-9
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