Agilent InfinityLab LC Series 1290 Infinity II Bio LC Chromatograph Quick Guide PDF

1290 Infinity II Bio LC

Agilent InfinityLab LC Series

System Manual and Quick Guide

1290 Infinity II Bio LC System Manual and Quick Guide

Notices

Document Information Document No: D0002753 Rev. B.00 Edition: 03/2021

Copyright Agilent Technologies, Inc. 2020-2021

No part of this manual may be repro- duced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written con- sent from Agilent Technologies, Inc. as governed by United States and interna- tional copyright laws.

Warranty The material contained in this document is provided as is, and is subject to being changed, without notice, in future edi- tions. Further, to the maximum extent permitted by applicable law, Agilent dis- claims all warranties, either express or implied, with regard to this manual and any information contained herein, includ- ing but not limited to the implied warran- ties of merchantability and fitness for a particular purpose. Agilent shall not be lia- ble for errors or for incidental or conse- quential damages in connection with the furnishing, use, or performance of this document or of any information con- tained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Restricted Rights Legend U.S. Government Restricted Rights. Soft- ware and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Soft- ware and technical data pursuant to FAR 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Depart- ment of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commer- cial Computer Software or Computer Software Documentation).

Safety Notices

CAUTION A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARN- ING notice until the indicated conditions are fully understood and met.

Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn, Germany

1290 Infinity II Bio LC System Manual and Quick Guide

In This Book

This book describes the Agilent 1290 Infinity II Bio LC System.

1 Introduction

This chapter gives an introduction to the Agilent 1290 Infinity II Bio LC, the underlying concepts and the features of the Agilent 1290 Infinity II Bio LC.

2 Install the Modules

This chapter provides information on how to install the modules and the capillaries.

3 Configuration Settings

This chapter describes how to configure the system.

4 Quick Start Guide

This chapter provides information on running an Agilent 1290 Infinity II Bio LC System.

5 Parts and Consumables

This chapter provides information on additional parts and consumables.

6 Appendix

This chapter provides addition information on safety, legal, and web.

1290 Infinity II Bio LC System Manual and Quick Guide 3

Contents

1 Introduction 5

Product Description 6 Features of the Agilent 1290 Infinity II Bio LC 7 System Components 8 Bio Materials 16 Optimize the Stack Configuration 25 Leak and Waste Handling 29

2 Install the Modules 35

Installing the System Modules 36 Integration Into the Network 37 Capillary and Tubing Connections in Flow Path 38

3 Configuration Settings 39

General Information on LAN Configuration 40 Instrument Configuration 41

4 Quick Start Guide 43

Best Practices 44 Prepare a Run 45 Check Out the System 51

5 Parts and Consumables 55

Tool Kit 56 Heat Exchanger for MCT 57 Valve Kits 58

6 Appendix 59

Safety 60 Agilent Technologies on Internet 67

4 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction

Product Description 6

Features of the Agilent 1290 Infinity II Bio LC 7

System Components 8 Bio Flexible Pump (G7131A) 9 Bio High-Speed Pump (G7132A) 10 Bio Multisampler (G7137A) 11 Multicolumn Thermostat with Bio Heat Exchangers 12 Diode Array Detector FS with Bio Flow Cell 13 Diode Array Detector with Bio Flow Cell 14 Variable Wavelength Detector with Bio Flow Cell 15

Bio Materials 16 Solvent Information 18

Optimize the Stack Configuration 25 Agilent A-Line LC Flex Bench 25 One Stack Configuration 27 Two Stack Configuration 28

Leak and Waste Handling 29 Waste Guidance 33 Waste Concept 34

This chapter gives an introduction to the Agilent 1290 Infinity II Bio LC, the underlying concepts and the features of the Agilent 1290 Infinity II Bio LC.

1290 Infinity II Bio LC System Manual and Quick Guide 5

1 Introduction Product Description

Product Description

The 1290 Infinity II Bio LC Systembinary or quaternaryUHPLC system, consisting of biocompatible material for use in biopharma (e.g., critical quality attributes) and other applications utilizing high-salt and extreme-pH conditions, ensures biomolecule integrity and robustness of the system.

Based on proven 1290 Infinity II technology, the system offers easy method transfer, also from legacy instrumentation, and reduced training effort. It provides excellent resolution and lowest dispersion at pressures up to 1300 bar. A range of bio flow cells is available for sensitive UV detection.

6 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Features of the Agilent 1290 Infinity II Bio LC

Features of the Agilent 1290 Infinity II Bio LC

Biocompatible solvent and sample flow path ensure integrity of biomolecules and minimize unwanted surface interaction

Flexibility for all applicationsthrough wide power, temperature, an automatically scalable injection ranges, and gradient options (binary and quaternary solvent mixing)

Lowest delay volume throughout the system enables superb chromatographic resolution and lowest dispersion

High salt tolerance and wide pH range offer enhanced flexibility and robustness for increased instrument uptime

Based on the proven 1290 Infinity II technology for easy method transfer, also from legacy instrumentation, and less training effort

Shallow microplate drawers take a maximum load of 6144 samples for unmatched sample capacity

Power range combines ultrahigh pressure up to 1300 bar and high analytical flow rates up to 5 mL/min for maximum UHPLC performance

Different bio accessories include a range of bio heat exchangers, bio capillary kits, bio loops, and analytical heads to cover all the different application needs for instrument versatility and efficiency

A wide range of sensitive optical detection capabilities with various flow cells for VWD, DAD, FLD, Bio MDS, or LC/MS detection for outstanding adaptability

1290 Infinity II Bio LC System Manual and Quick Guide 7

1 Introduction System Components

System Components

The Agilent 1290 Infinity II Bio LC system consists of the following components:

Bio High-Speed Pump (G7132A) or Bio Flexible Pump (G7131A)

Bio Multisampler (G7137A)

Multicolumn Thermostat with Bio Heat Exchanger

Diode Array Detector and Variable Wavelength Detector with respective Bio flow cell

Solvent Cabinet

The Agilent 1290 Infinity II Bio LC is described in more detail in the following sections. All modules are stackable, see Optimize the Stack Configuration on page 25.

For specifications, please refer to the individual module user documentation.

8 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction System Components

Bio Flexible Pump (G7131A) The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of biocompatible material, designed for use in biopharma and other applications utilizing high-salt and extreme-pH conditions. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.

The 1290 Infinity II Bio Flexible Pump combines exceptional performance, method compatibility, and simplified operation with outstanding flexibility in automated gradient formation and solvent blending. Intelligent System Emulation Technology (ISET) allows you to seamlessly transfer existing methods from different instruments.

Figure 1 Overview of the Flexible Pump

Status indicator

Pump head

MCGV

Inlet weaver

Power switch Leak drain

Pressure sensor

1290 Infinity II Bio LC System Manual and Quick Guide 9

1 Introduction System Components

Bio High-Speed Pump (G7132A) The 1290 Infinity II Bio High-Speed Pump is a binary UHPLC pump consisting of biocompatible material for use in biopharma (critical quality attributes) and other applications utilizing high salt and extreme pH conditions. It uses high-pressure blending from two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.

The 1290 Infinity II Bio High-Speed Pump has an exceptionally low delay volume, achieving high throughput and highest resolution for the most demanding applications, giving you highest confidence in your results. It is the ideal front end for LC/MS applications.

Figure 2 Overview of the High-Speed Pump

Status indicator

Leak drain

Power switch

Pressure sensor

Pump head B

Pump head A

Seal wash pump

SSV

Jet weaver

10 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction System Components

Bio Multisampler (G7137A) The 1290 Infinity II Bio Multisampler, with its biocompatible sample flow path, is perfectly suited for biomolecule analysis, such as critical quality attributes (CQAs), ensuring integrity of biomolecules and minimizing unwanted surface interaction. It is a multipurpose autosampler that handles vials and microplates, and is optimized for highest chromatographic performance.

Injecting at pressures up to 1300 bar, the Bio Multisampler is a compact module with a capacity of up to 6144 samples, all within the footprint of an Agilent LC stack. For temperature-sensitive samples, a highly efficient compressor-based thermostatting device can be added.

Figure 3 Overview of the Multisampler

Status indicator

Needle assembly

Hotel drawer

Leak drain

Power switch

1290 Infinity II Bio LC System Manual and Quick Guide 11

1 Introduction System Components

Multicolumn Thermostat with Bio Heat Exchangers The Agilent 1290 Infinity II Multicolumn Thermostat (MCT) facilitates precise column thermostatting over a broad temperature range with cooling down to 20 C below ambient temperature and heating up to 110 C.

This capability provides high flexibility for optimized speed and enhanced selectivity of LC separations. Exchangeable ultra-high-pressure valves enable a wide range of applications such as column selection from eight columns in a single MCT, sample preparation for analyte enrichment or matrix removal, alternating column regeneration and many more.

The MCT matches perfectly with all InfinityLab LC Series systems and can also be combined with previous 1260 and 1290 Infinity Series modules.

Figure 4 Overview of the Multicolumn Thermostat

Power switch

Status indicator

Valve

Leak drain

12 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction System Components

Diode Array Detector FS with Bio Flow Cell The Agilent 1290 Infinity II Diode Array Detector FS (fixed slit) is based on the Agilent Max-Light cartridge cell with optofluidic waveguides that improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects.

With typical detector noise levels of < 0.6 AU/cm the 60 mm flow cell gives up to 10 times higher sensitivity than detectors with conventional flow cells.

Any compromising refractive index and thermal effects are almost completely eliminated, resulting in significantly less baseline drift for more reliable and precise peak integration.

For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 120 Hz.

Figure 5 Overview of the detector

Status indicator

Lamp housing cover

Cartridge Flow Cell

Power switch

Leak drain

Air inlet

1290 Infinity II Bio LC System Manual and Quick Guide 13

1 Introduction System Components

Diode Array Detector with Bio Flow Cell The Agilent 1290 Infinity II Diode Array Detector (DAD) is based on the Agilent Max-Light cartridge cell with optofluidic waveguides that improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects.

With typical detector noise levels of < 0.6 AU/cm the 60 mm flow cell gives up to 10 times higher sensitivity than detectors with conventional flow cells.

Any compromising refractive index and thermal effects are almost completely eliminated, resulting in significantly less baseline drift for more reliable and precise peak integration.

For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 240 Hz.

Figure 6 Overview of the detector

Status indicator

Lamp housing cover

Cartridge Flow Cell

Power switch

Leak drain

Air inlet

14 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction System Components

Variable Wavelength Detector with Bio Flow Cell The Agilent 1290 Infinity II Variable Wavelength Detector (VWD) is the most sensitive and fastest detector in its class.

Time-programmable wavelength switching provides sensitivity and selectivity for your applications.

More sample information can be acquired in the dual wavelength mode.

Low detector noise (<1.5 AU) and baseline drift (<110-4 AU/h) facilitates precise quantification of trace levels components.

High productivity can be achieved with fast analysis at up to 240 Hz data rates.

Figure 7 Overview of the detector

Status indicator

Flow cell

Lamp housing window and air inlet

Power switch

Leak drain

1290 Infinity II Bio LC System Manual and Quick Guide 15

1 Introduction Bio Materials

Bio Materials

For the 1290 Infinity II Bio LC System, Agilent Technologies uses highest quality materials in the flow path (also referred to as wetted parts). Life scientists accept these materials, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range. To enable chromatography at very high pressures, while maintaining inertness the metal alloy MP35N is used instead of stainless steel throughout the system.

The MP35N is a nonmagnetic, nickel-cobalt-chromium-molybdenum alloy with an excellent resistance to sulfation, oxidation, saline solutions, and most mineral acids. Its superior properties guaranty reliable performance, even under UHPLC conditions.

16 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Bio Materials

Table 1 Used Bio-compatible materials

Module Materials

Agilent 1290 Infinity Bio High-Speed Pump (G7132A)

MP35N, gold, ceramic, ruby, PTFE, PEEK, UHMW-PE, tantalum, TFE/PDD copolymer, PFA, FEP, FFKM, ETFE, DLC coated MP35N

Agilent 1290 Infinity Bio Flexible Pump (G7131A) MP35N, gold, ceramic, ruby, PEEK, UHMW-PE, tantalum, TFE/PDD copolymer, PFA, FEP, titanium, DLC coated MP35N, FFKM, ETFE

Agilent 1290 Infinity Bio Multisampler (G7137A) Standard: MP35N, gold, DLC coated MP35N, PEEK, UHMW-PE Multi Wash: PEEK, FFKM, titanium, PTFE, PPS, MP35N, gold, DLC coated MP35N, UHMW-PE

Bio-compatible Flow Cells:

Max-Light Cartridge Cell LSS (10 mm) (G7117-60020) (for Agilent 1290 Infinity II DAD (G7117A/B))

MP35N, fused silica, PEEK

Bio standard flow cell VWD, 10 mm 14 l, RFID (G1314-60188) (for Agilent 1290 Infinity II VWD (G7114B)

Sapphire, MP35N, FEP

Bio micro flow cell VWD, 3 mm, 2 l, RFID (G1314-60189) (for Agilent 1290 Infinity II VWD (G7114B)

Sapphire, MP35N, FEP

Bio-compatible Heat Exchangers, Valves and Capillaries:

Quick Connect Bio Heat Exchanger Standard Flow (G7116-60071), Quick Connect Bio Heat Exchanger High Flow (G7116-60081), Quick Connect Heat Exchanger 1290 Bio ULD (G7116-60091) (for Agilent 1290 Infinity II Multicolumn Thermostat (G7116B))

MP35N

2-position/10-port valve, bio 1300 bar (G5641A) MP35N coated with DLC, PEEK

12ps/13pt valve, bio-inert (G4235A) PEEK, ceramic (Al2O3 based)

Bio connection capillaries MP35N

NOTE To ensure optimum bio-compatibility of your Agilent 1290 Infinity II Bio LC System, do not include non-Bio standard modules or parts to the flow path. Do not use any parts that are not labeled as Agilent Bio. For solvent compatibility of these materials, see General Information about Solvent/Material Compatibility on page 18

1290 Infinity II Bio LC System Manual and Quick Guide 17

1 Introduction Bio Materials

Solvent Information Observe the following recommendations on the use of solvents.

Follow the recommendations for avoiding the growth of algae, see the pump manuals.

Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 m filters.

Avoid or minimize the use of solvents that may corrode parts in the flow path. Consider specifications for the pH range given for different materials such as flow cells, valve materials etc. and recommendations in subsequent sections.

General Information about Solvent/Material Compatibility

Materials in the flow path are carefully selected based on Agilents experiences in developing highest-quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.

Disclaimer

Subsequent data was collected from external resources and is meant as a reference. Agilent cannot guarantee the correctness and completeness of such information. Data is based on compatibility libraries, which are not specific for estimating the long-term life time under specific but highly variable conditions of UHPLC systems, solvents, solvent mixtures and samples. Information can also not be generalized due to catalytic effects of impurities like metal ions, complexing agents, oxygen etc. Apart from pure chemical corrosion, other effects like electro corrosion, electrostatic charging (especially for non-conductive organic solvents), swelling of polymer parts etc. need to be considered. Most data available refers to room temperature (typically 20 25 C, 68 77 F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials.

18 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Bio Materials

MP35N

MP35N is a nonmagnetic, nickel-cobalt-chromium-molybdenum alloy demonstrating excellent corrosion resistance (for example, against nitric and sulfuric acids, sodium hydroxide, and seawater) over a wide range of concentrations and temperatures. In addition, this alloy shows exceptional resistance to high-temperature oxidation. Due to excellent chemical resistance and toughness, the alloy is used in diverse applications: dental products, medical devices, nonmagnetic electrical components, chemical and food processing equipment, marine equipment. Treatment of MP35N alloy samples with 10 % NaCl in HCl (pH 2.0) does not reveal any detectable corrosion. MP35N also demonstrates excellent corrosion resistance in a humid environment. Although the influence of a broad variety of solvents and conditions has been tested, users should keep in mind that multiple factors can affect corrosion rates, such as temperature, concentration, pH, impurities, stress, surface finish, and dissimilar metal contacts.

Polyphenylene Sulfide (PPS)

Polyphenylene sulfide has outstanding stability even at elevated temperatures. It is resistant to dilute solutions of most inorganic acids, but it can be attacked by some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids, such as sulfuric acid and phosphoric acid, have little effect on polyphenylene sulfide, but at high concentrations and temperatures, they can still cause material damage. Nonoxidizing organic chemicals generally have little effect on polyphenylene sulfide stability, but amines, aromatic compounds, and halogenated compounds may cause some swelling and softening over extended periods of time at elevated temperatures. Strong oxidizing acids, such as nitric acid (> 0.1 %), hydrogen halides (> 0.1 %), peroxy acids (> 1 %), or chlorosulfuric acid degrade polyphenylene sulfide. It is not recommended to use polyphenylene sulfide with oxidizing material, such as sodium hypochlorite and hydrogen peroxide. However, under mild environmental conditions, at low concentrations and for short exposure times, polyphenylene sulfide can withstand these chemicals, for example, as ingredients of common disinfectant solutions.

PEEK

PEEK (Polyether-Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is therefore the material of choice for UHPLC and biochemical instrumentation.

It is stable in the specified pH range (for the Bio-Inert LC system: pH 1 13, see bio-inert module manuals for details), and inert to many common solvents.

1290 Infinity II Bio LC System Manual and Quick Guide 19

1 Introduction Bio Materials

There is still a number of known incompatibilities with chemicals such as chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %, sulfuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogens or aqueous halogen solutions, phenol and derivatives (cresols, salicylic acid, and so on).

When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions, normal PEEK capillaries are sensitive to high pressure. Therefore, Agilent uses stainless steel cladded PEEK capillaries in bio-inert systems. The use of stainless steel cladded PEEK capillaries keeps the flow path free of steel and ensures pressure stability up to 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK.

Polyimide

Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent.

Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like ammonia (e.g. ammonium salts in basic conditions) or acetates.

Polyethylene (PE)

Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow piston and wash seals, which are used in 1290 Infinity pumps, 1290 Infinity II pumps, the G7104C and for normal phase applications in 1260 Infinity pumps.

Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12.5. It is compatible with many organic solvents used in chromatographic systems like methanol, acetonitrile and isopropanol. It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar.

Tantalum (Ta)

Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides.

20 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Bio Materials

Stainless Steel (SST)

Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by acids below pH 2.3. It can also corrode in following solvents: Solutions of alkali halides, their respective acids (for example, lithium iodide,

potassium chloride, and so on) and aqueous solutions of halogens. High concentrations of inorganic acids like nitric acid, sulfuric acid and

organic solvents especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel).

Halogenated solvents or mixtures which form radicals and/or acids, for example: 2 CHCl3 + O2 2 COCl2 + 2 HCl

This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.

Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, diisopropylether). Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides.

Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel.

Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid).

Mixtures of carbon tetrachloride with isopropanol or THF.

Titanium (Ti)

Titanium is highly resistant to oxidizing acids (for example, nitric, perchloric and hypochlorous acid) over a wide range of concentrations and temperatures. This is due to a thin oxide layer on the surface, which is stabilized by oxidizing compounds. Non-oxidizing acids (for example, hydrochloric, sulfuric and phosphoric acid) can cause slight corrosion, which increases with acid concentration and temperature. For example, the corrosion rate with 3 % HCl (about pH 0.1) at room temperature is about 13 m/year. At room temperature, titanium is resistant to concentrations of about 5 % sulfuric acid (about pH 0.3). Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates. Titanium is sensitive to acidic metal chlorides like FeCl3 or CuCl2. Titanium is subject to corrosion in anhydrous methanol, which can be avoided by adding a small amount of water (about 3 %). Slight corrosion is possible with ammonia > 10 %.

1290 Infinity II Bio LC System Manual and Quick Guide 21

1 Introduction Bio Materials

Diamond-Like Carbon (DLC)

Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Fused silica and Quartz (SiO2)

Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows. It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides. It is corroded by strong bases and should not be used above pH 12 at room temperature. The corrosion of flow cell windows can negatively affect measurement results. For a pH greater than 12, the use of flow cells with sapphire windows is recommended.

Gold

Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia.

Zirconium Oxide (ZrO2)

Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Platinum/Iridium

Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

22 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Bio Materials

Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF)

Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons.

TFE/PDD copolymer tubings, which are used in all Agilent degassers except G1322A/G7122A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel. They have limited life time in the presence of hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let dry out the chamber. For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off.

The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible with the solvent DMF (dimethyl formamide).

Sapphire, Ruby and Al2O3-based ceramics

Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

1290 Infinity II Bio LC System Manual and Quick Guide 23

1 Introduction Bio Materials

Flow Cell

To protect optimal functionality of your flow cell:

If the flow cell is transported while temperatures are below 5 C, it must be ensured that the cell is filled with alcohol to avoid damage by freezing water.

Aqueous solvents in the flow cell can build up algae. Therefore, do not leave aqueous solvents sitting in the flow cell. Add a small percentage of organic solvents (for example, about 5 % of acetonitrile or methanol).

24 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Optimize the Stack Configuration

Optimize the Stack Configuration

To ensure safe operation and optimum performance of an Agilent InfinityLab LC System, Agilent Technologies prescribe stack configurations. The following configurations are possible:

InfinityLab Flex Bench

Single Stack (maximal 4 modules, in a bench rack or directly on the bench)

InfinityLab Benchtop, providing more flexibility

Two Stacks (in a bench rack or directly on the bench)

Agilent A-Line LC Flex Bench Agilent recommends to use the InfinityLab Flex Bench for all Agilent LC systems.

Main features:

Increases flexibility in the lab

Safe moving of LC

Easy stack customization

Included waste management

1290 Infinity II Bio LC System Manual and Quick Guide 25

1 Introduction Optimize the Stack Configuration

Figure 8 Agilent InfinityLab Flex Bench

Solvent cabinet

Detector

Sampler

Pump

Handle grip

Shelves

Wheel locking brake

Container for waste bottles

Column compartment

26 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Optimize the Stack Configuration

One Stack Configuration Ensure optimum performance by stacking the modules as shown exemplarily in Figure 9 on page 27. This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required.

Figure 9 Single stack configuration (bench installation, example shows a Multisampler)

Detector

Solvent cabinet

Pump

Sampler

Column compartment

1290 Infinity II Bio LC System Manual and Quick Guide 27

1 Introduction Optimize the Stack Configuration

Two Stack Configuration To avoid excessive height of the stack (for example when using the system in combination with an additional detector) it is recommended to form two stacks.

Figure 10 Two stack configuration (bench installation, example shows a Multisampler)

Solvent cabinet

Sampler

Pump

Detectors

Column compartment

28 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Leak and Waste Handling

Leak and Waste Handling

The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.

The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).

All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other. Waste tubes are guided through a channel on the right hand side of the instrument, keeping the front access clear from tubes.

The leak plane provides leak management by catching all internal liquid leaks, guiding them to the leak sensor for leak detection, and passing them on to the next module below, if the leak sensor fails. The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached.

Solvent and condensate is guided through the waste channel into the waste container:

from the detector's flow cell outlet

from the Multisampler needle wash port

from the Sample Cooler or Sample Thermostat (condensate)

from the pump's Seal Wash Sensor (if applicable)

from the pump's Purge Valve or Multipurpose Valve

1290 Infinity II Bio LC System Manual and Quick Guide 29

1 Introduction Leak and Waste Handling

Figure 11 Infinity II Leak Waste Concept (Flex Bench installation)

30 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Leak and Waste Handling

Figure 12 Infinity II Single Stack Leak Waste Concept (bench installation)

1290 Infinity II Bio LC System Manual and Quick Guide 31

1 Introduction Leak and Waste Handling

Figure 13 Infinity II Two Stack Leak Waste Concept (bench installation)

The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container.

32 1290 Infinity II Bio LC System Manual and Quick Guide

1 Introduction Leak and Waste Handling

Waste Guidance

NOTE The waste drainage must go straight into the waste containers. The waste flow must not be restricted at bends or joints.

1290 Infinity II Bio LC System Manual and Quick Guide 33

1 Introduction Leak and Waste Handling

Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with

4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid.

34 1290 Infinity II Bio LC System Manual and Quick Guide

2 Install the Modules

Installing the System Modules 36

Integration Into the Network 37

Capillary and Tubing Connections in Flow Path 38

This chapter provides information on how to install the modules and the capillaries.

1290 Infinity II Bio LC System Manual and Quick Guide 35

2 Install the Modules Installing the System Modules

Installing the System Modules

For details of installation procedures for the modules, refer to the individual module manuals. These manuals also contain information on specifications, maintenance and parts.

36 1290 Infinity II Bio LC System Manual and Quick Guide

2 Install the Modules Integration Into the Network

Integration Into the Network

For network integration of your system refer to user manuals of your modules (chapter LAN Configuration).

1290 Infinity II Bio LC System Manual and Quick Guide 37

2 Install the Modules Capillary and Tubing Connections in Flow Path

Capillary and Tubing Connections in Flow Path

Depending on the system configuration, you may need capillaries of different lengths. To achieve optimal LC results, the following Bio capillaries are available:

See module manuals for module-internal capillary and tubing connections.

Table 2 Capillary connections for 1290 Infinity II Bio LC

p/n From To

Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump

Capillary MP35N 0.17 mm x 500 mm, SI/SI (5500-1419) Pump Multisampler

Capillary MP35N 0.12 mm x 500 mm SI/SI (5500-1279) Multisampler MCT

Quick Connect Capillary MP35N 0.12 mm x 105 mm (5500-1578)

MCT Heat Exchanger Column

Quick Turn Capillary MP35N 0.12 mm x 280 mm (5500-1596) Column/MCT Valve Detector (DAD)

Quick Turn Capillary MP35N 0.12 mm x 500 mm (5500-1598) Column/MCT Valve Detector (VWD)

Waste accessory kit (5062-8535) VWD Waste

Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462) DAD/FLD Waste

Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712) Detector Fraction Collector

38 1290 Infinity II Bio LC System Manual and Quick Guide

3 Configuration Settings

General Information on LAN Configuration 40

Instrument Configuration 41

This chapter describes how to configure the system.

1290 Infinity II Bio LC System Manual and Quick Guide 39

3 Configuration Settings General Information on LAN Configuration

General Information on LAN Configuration

LAN configuration is executed from the module with direct LAN connection to the controller software. This must be the module (usually the detector) with the highest data rate.

40 1290 Infinity II Bio LC System Manual and Quick Guide

3 Configuration Settings Instrument Configuration

Instrument Configuration

Example shows an instrument configuration with a Diode Array Detector.

1 Set the switches of the Configuration switch at the rear of the module:

a All switches DOWN: module uses the default IP address 192.168.254.11.

b Switch 4 UP and others DOWN: module uses DHCP.

c Switch 5 UP and others DOWN: modules uses STORED address.

1290 Infinity II Bio LC System Manual and Quick Guide 41

3 Configuration Settings Instrument Configuration

2 Enter the setup information (MAC1 / IP address and/or Instrument Name).

a Agilent OpenLab ChemStation (Configure Instrument):

b Lab Advisor (Instrument Overview - Add Instrument):

1 MAC address can only be used in DHCP DIP-switch configuration.

42 1290 Infinity II Bio LC System Manual and Quick Guide

4 Quick Start Guide

Best Practices 44

Prepare a Run 45

Check Out the System 51 Checkout Method 51 Setup the Checkout Method 54

This chapter provides information on running an Agilent 1290 Infinity II Bio LC System.

1290 Infinity II Bio LC System Manual and Quick Guide 43

4 Quick Start Guide Best Practices

Best Practices

For best practices, refer to the Agilent Information Center on Agilent InfinityLab LC Series User Documentation (G4800-64600), Best Practices for Using an Agilent LC System (01200-90090), or the 1290 Infinity II Bio and 1260 Infinity II Prime Bio LC Quick Reference Sheet (G7132-90110).

44 1290 Infinity II Bio LC System Manual and Quick Guide

4 Quick Start Guide Prepare a Run

Prepare a Run

This procedure examplarily shows how to prepare a run. Parameters as shown in the screenshots may vary, depending on the system installed.

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 C (392 F). Do not use solvents with a boiling point below 56 C (133 F).

Avoid high vapor concentrations. Keep the solvent temperature at least 40 C (72 F) below the boiling point of the solvent used. This includes the solvent temperature in the sample compartment. For the solvents methanol and ethanol keep the solvent temperature at least 25 C (45 F) below the boiling point.

Do not operate the instrument in an explosive atmosphere.

Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

Reduce the volume of substances to the minimum required for the analysis.

Never exceed the maximum permissible volume of solvents (8 L) in the solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container.

Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

1290 Infinity II Bio LC System Manual and Quick Guide 45

4 Quick Start Guide Prepare a Run

1 Switch on the detector.

2 Fill the solvent bottles with adequate solvents for your application.

3 Place solvent tubings with bottle head assemblies into the solvent bottles.

4 Place solvent bottles into the solvent cabinet.

5 Solvent bottle filling dialog (in the software).

46 1290 Infinity II Bio LC System Manual and Quick Guide

4 Quick Start Guide Prepare a Run

6 Purge the pump.

NOTE For details on priming and purging, refer to the technical note Best Practices for Using an Agilent LC System.

1290 Infinity II Bio LC System Manual and Quick Guide 47

4 Quick Start Guide Prepare a Run

7 Change solvent type if necessary.

8 Choose the tray format of the sampler.

9 Add a new column.

48 1290 Infinity II Bio LC System Manual and Quick Guide

4 Quick Start Guide Prepare a Run

10 Enter the column information.

11 Select the column in the Method settings of the column compartment.

1290 Infinity II Bio LC System Manual and Quick Guide 49

4 Quick Start Guide Prepare a Run

12 Set the detector parameters according to the needs of your method.

50 1290 Infinity II Bio LC System Manual and Quick Guide

4 Quick Start Guide Check Out the System

Check Out the System

Checkout Method Configuration:

Pump

Multisampler

Multicolumn Thermostat

Diode Array Detector

Variable Wavelength Detector

The RRLC checkout sample (5188-6529) serves as standard for systems with 600 bar or higher and contains 100 ng/L each of nine components dissolved in water / acetonitrile (65/35). The nine components are:

Acetanilide

Acetophenone

Propiophenone

Butyrophenone

Benzophenone

Valerophenone

Hexanophenone

Heptanophenone

Octanophenone

NOTE Find the correct settings for the individual modules here:

Table 3 on page 52

Table 4 on page 52

Table 5 on page 52

Table 6 on page 53

Table 7 on page 53

1290 Infinity II Bio LC System Manual and Quick Guide 51

4 Quick Start Guide Check Out the System

Table 3 Checkout method parameter settings Bio High-Speed Pump (G7132A) and Bio Flexible

Pumps (G7131A/C)

Parameter Value

Flow 0.8 mL/min

Solvent A Water

Solvent B ACN

Compressibility Use solvent types

Composition 40 %B (ACN)

Composition 60 %A (Water)

Stoptime 10 min

Pressure Limit 600 bar

Minimum Stroke Automatic

Timetable 2.5 min, 80 %B

Table 4 Checkout method parameter settings Bio Multisampler (G7137A)

Parameter Value

Injection 1 L

Stoptime as pump

Draw speed 100 L/min

Table 5 Checkout method parameter settings Multicolumn Thermostat (G7116A/B)

Parameter Value

Temperature (left) 40 C

Temperature (right) combined

Stoptime as pump

52 1290 Infinity II Bio LC System Manual and Quick Guide

4 Quick Start Guide Check Out the System

Table 6 Checkout method parameter settings Diode Array Detectors (G7117A/B)

Parameter Value

Signal A 254/4 nm

Ref A 360/100 nm

Peakwidth 40 Hz

Stoptime as pump

Spectrum None

Autobalance Prerun

Table 7 Checkout method parameter settings Variable Wavelength Detector (G7114B)

Parameter Value

Wavelength 254 nm

Peakwidth 40 Hz

Stoptime as pump

Autobalance Prerun

1290 Infinity II Bio LC System Manual and Quick Guide 53

4 Quick Start Guide Check Out the System

Setup the Checkout Method

1 Turn on the lamp.

2 Load the the default method DEF_LC.M

3 Change the method and timetable settings for the pump.

4 Change the method settings for the sampler.

5 Change the method settings for the column thermostat.

6 Change the method settings for the detector.

7 Save the method as GRAD-1.M.

8 Equilibrate the system for 10 min under checkout conditions.

9 Run and evaluate the checkout method.

NOTE The setup of the checkout method in this procedure is an example. For the individual module parameters, see Checkout Method on page 51.

54 1290 Infinity II Bio LC System Manual and Quick Guide

5 Parts and Consumables

Tool Kit 56

Heat Exchanger for MCT 57

Valve Kits 58

This chapter provides information on additional parts and consumables.

1290 Infinity II Bio LC System Manual and Quick Guide 55

5 Parts and Consumables Tool Kit

Tool Kit

The HPLC System Tool Kit contains all tools to operate and maintain an Agilent LC system.

The High quality tools are packed in an appealing tool case.

Figure 14 Tool kit

8710-1924 8710-2409 8710-0510 8710-0510 5023-2500 8710-1534

Box with: 9301-0411 (Syringe, Plastic) 9301-1337 (Syringe Adapter) 0100-1710 (Mounting tool for flangeless nut) 0100-1681 (Adapter luer/barb) 01018-23702 (Seal Insert tool) 5067-6127 (Blank Nut V) 5043-1361

(Hex Key Set Driver)

8710-2394 (Hex Key 9/64, 15cm)

5023-2504 (Hex Driver SW-4 slitted)

5023-2499 (Hex Key Set)

5023-2502

35/ slitted) (Hex Driver SW-6,

5023-2503

SW-5 slitted) (Hex Driver

5023-2653 (Hex Key 3/32)

5023-3088

5023-3089 Torx Key Set (T8,T9,T10,T15,T20,T25)

5023-3138 (Reversible Screwdriver)

56 1290 Infinity II Bio LC System Manual and Quick Guide

5 Parts and Consumables Heat Exchanger for MCT

Heat Exchanger for MCT

For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.

p/n Description

G7116-60071 Quick Connect Bio Heat Exchanger Standard Flow

G7116-60081 Quick Connect Bio Heat Exchanger High Flow

G7116-60091 Quick Connect Bio Heat Exchanger Ultra Low Dispersion

1290 Infinity II Bio LC System Manual and Quick Guide 57

5 Parts and Consumables Valve Kits

Valve Kits

G5641A 2-position/10-port valve bio 1300 bar

G4235A Solvent Selection Valve Head, Bio-inert - Stand Alone Valve

For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.

Table 8 G5641A 2-position/10-port valve bio 1300 bar

Description Amount Unit Comment

2-position/10-port bio valve head, 1300 bar (5067-6682) 1 ea

Bio 2/10 Capillary Kit (5013-0002) 1 ea

Table 9 G4235A Solvent Selection Valve Head, Bio-inert - Stand Alone Valve

Description Amount Unit Comment

12 position/13 port selector valve head, 210 bar, bio-inert (5067-4159) 1 ea

Solvent selection tubing kit, 4 solvents (5067-4601) up to 4 ea optional

58 1290 Infinity II Bio LC System Manual and Quick Guide

6 Appendix

Safety 60 General Safety Information 60 Safety Standards 60 General 60 Before Applying Power 61 Ground the Instrument 61 Do Not Operate in an Explosive Atmosphere 62 Do Not Remove the Instrument Cover 62 Do Not Modify the Instrument 63 In Case of Damage 63 Solvents 64 Symbols 65

Agilent Technologies on Internet 67

This chapter provides addition information on safety, legal, and web.

1290 Infinity II Bio LC System Manual and Quick Guide 59

6 Appendix Safety

Safety

General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.

Safety Standards This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards.

General Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions.

WARNING Ensure the proper usage of the equipment.

The protection provided by the equipment may be impaired.

The operator of this instrument is advised to use the equipment in a manner as specified in this manual.

60 1290 Infinity II Bio LC System Manual and Quick Guide

6 Appendix Safety

Before Applying Power

Ground the Instrument

WARNING Wrong voltage range, frequency or cabling

Personal injury or damage to the instrument

Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Make all connections to the unit before applying power.

NOTE Note the instrument's external markings described under Symbols on page 65.

WARNING Missing electrical ground

Electrical shock

If your product is provided with a grounding type power plug, the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard.

The ground pin must be firmly connected to an electrical ground (safety ground) terminal at the power outlet. Any interruption of the protective (grounding) conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury.

1290 Infinity II Bio LC System Manual and Quick Guide 61

6 Appendix Safety

Do Not Operate in an Explosive Atmosphere

Do Not Remove the Instrument Cover

WARNING Presence of flammable gases or fumes

Explosion hazard

Do not operate the instrument in the presence of flammable gases or fumes.

WARNING Instrument covers removed

Electrical shock

Do Not Remove the Instrument Cover

Only Agilent authorized personnel are allowed to remove instrument covers. Always disconnect the power cables and any external circuits before removing the instrument cover.

62 1290 Infinity II Bio LC System Manual and Quick Guide

6 Appendix Safety

Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product. Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained.

In Case of Damage

WARNING Damage to the module

Personal injury (for example electrical shock, intoxication)

Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel.

1290 Infinity II Bio LC System Manual and Quick Guide 63

6 Appendix Safety

Solvents

WARNING Toxic, flammable and hazardous solvents, samples and reagents

The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 C (392 F). Do not use solvents with a boiling point below 56 C (133 F).

Avoid high vapor concentrations. Keep the solvent temperature at least 40 C (72 F) below the boiling point of the solvent used. This includes the solvent temperature in the sample compartment. For the solvents methanol and ethanol keep the solvent temperature at least 25 C (45 F) below the boiling point.

Do not operate the instrument in an explosive atmosphere.

Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

Reduce the volume of substances to the minimum required for the analysis.

Never exceed the maximum permissible volume of solvents (8 L) in the solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container.

Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

NOTE For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet.

64 1290 Infinity II Bio LC System Manual and Quick Guide

6 Appendix Safety

Symbols

Table 10 Symbols

The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up.

Sample Cooler unit is designed as vapor-compression refrigeration system. Contains fluorinated greenhouse gas (refrigerant) according to the Kyoto protocol. For specifications of refrigerant, charge capacity, carbon dioxide equivalent (CDE), and global warming potential (GWP) see instrument label.

Flammable Material For Sample Thermostat which uses flammable refrigerant consult Agilent Information Center / User Manual before attempting to install or service this equipment. All safety precautions must be followed.

Confirms that a manufactured product complies with all applicable European Community directives. The European Declaration of Conformity is available at: http://regulations.corporate.agilent.com/DoC/search.htm

Manufacturing date.

Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position

Pacemaker Magnets could affect the functioning of pacemakers and implanted heart defibrillators. A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets.

1290 Infinity II Bio LC System Manual and Quick Guide 65

6 Appendix Safety

Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

Indicates a pinching or crushing hazard

Indicates a piercing or cutting hazard.

Table 10 Symbols

WARNING A WARNING

alerts you to situations that could cause physical injury or death.

Do not proceed beyond a warning until you have fully understood and met the indicated conditions.

CAUTION A CAUTION

alerts you to situations that could cause loss of data, or damage of equipment.

Do not proceed beyond a caution until you have fully understood and met the indicated conditions.

66 1290 Infinity II Bio LC System Manual and Quick Guide

6 Appendix Agilent Technologies on Internet

Agilent Technologies on Internet

For the latest information on products and services visit our worldwide web site on the Internet at:

http://www.agilent.com

1290 Infinity II Bio LC System Manual and Quick Guide 67

In This Book

This manual contains technical reference information about the Agilent 1290 Infinity II Bio LC System.

The manual describes the following:

introduction,

install the modules,

configuration settings,

quick start guide,

parts and consumables.

www.agilent.com

Agilent T