Contents

Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide PDF

Download download
Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide PDF
Agilent Manual Agilent Analyzer Manual Agilent 5300 Fragment Manual Agilent 96-Capillary Arrays Manual Agilent Analyzer Quick Guide
Pages 4
Year 2018
Language(s)
English en
1 of 4
1 of 4

Summary of Content for Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide PDF

Page 1

Agilent CRISPR Discovery Gel Kit 5300 & 5400 Fragment Analyzer Systems 96-Capillary Arrays

Quick Guide For Research Use Only. Not for use in diagnostic procedures.

Kit Specifications ................................................................................................................................ 1

Kit Components ................................................................................................................................... 2

Analysis Protocol ................................................................................................................................ 2

Gel Preparation .................................................................................................................................. 2

Instrument and Sample Preparation ............................................................................................. 2

Fragment Analyzer Software Operating Procedure ................................................................... 4

Technical Support and Further Information ................................................................................... 4

Kit Specifications

Specifications Description

DNA sizing range 100 bp 6,000 bp

Separation resolution 8 bp @ 500 bp (33-55 Array)

DNA sizing precision1 2% CV

DNA sizing accuracy1 5% or better

DNA fragment concentration range 0.005 ng/L 2 ng/L input DNA (adjustable by dilution of sample)

1 Results using DNA ladder or DNA fragment standards initially prepared with 1x TE Buffer

Page 2

Agilent CRISPR Discovery Gel Kit Quick Guide 2

Kit Components

Agilent CRISPR Discovery Gel kit

Part Number Name

DNF-810 dsDNA 810 Gel

DNF-600-U030 Intercalating Dye

DNF-355 5x 930 dsDNA Inlet Buffer (Dilute to 1x)

DNF-475 5x Capillary Conditioning Solution (Dilute to 1x)

FA-MRK915F-0003 Markers, 1 bp & 6000 bp

FS-SLR480-0001 MDK DNA Ladder

DNF-494 Dilution Buffer 0.1x TE

FS-SMO15 Mineral Oil Dropper Bottle

GP-440-0100 Capillary Storage Solution (sold separately)

Analysis Protocol

Gel preparation

Prepare gel/dye mixture for 5300 (96-Capillary) & 5400 Fragment Analyzer systems: # of samples to be analyzed1 Volume of Intercalating Dye Volume of gel

96 4.0 L 40 mL

192 8.0 L 80 mL

288 12.0 L 120 mL

384 16.0 L 160 mL

480 20.0 L 200 mL

1 Typically one sample well per separation is dedicated to the ladder.

Instrument and sample preparation

1 Mix fresh gel and dye. Refill 1x Capillary Conditioning Solution as needed.

Page 3

Agilent CRISPR Discovery Gel Kit Quick Guide 3

2 Place a fresh 1x 930 dsDNA Inlet Buffer tray on the Fragment Analyzer system. Fill entire tray (1.0 mL/well). Replace 1x 930 dsDNA Inlet Buffer daily.

3 Place Capillary Storage Solution in sample tray 3. Fill 100 L/well. Replace Capillary Storage Solution every 2-4 weeks.

4 Place marker plate in marker drawer location. Fill entire tray (30 L/well + mineral oil overlay).

5 Mix samples with Diluent Buffer 0.1x TE in sample plate. Place 24 L of MDK DNA Ladder (ready to use; no dilution) into well H12.

WARNING

Working with Chemicals The handling of reagents and chemicals might hold health risks.

Refer to product material safety datasheets for further chemical and biological safety information.

Follow the appropriate safety procedures such as wearing goggles, safety gloves and protective clothing

Page 4

Fragment Analyzer software operating procedure

1 Select Tray to run for 96-Cap. 2 Enter Sample ID and Tray ID (optional). 3 Select Add to Queue, select the CRP-910-(33 or 55) CRISPR Discovery method from the

dropdown menu. 4 Enter Tray Name, Folder Prefix, and Notes (optional). 5 Select OK to add method to the queue. 6 Select to start the separation.

NOTE Please refer to the Kit Guide for additional details.

Technical Support and Further Information

For technical support, please visit www.agilent.com.

It offers useful information, support and current developments about the products and technology.

www.agilent.com Agilent Technologies, Inc. 2018

Manualsnet FAQs

If you want to find out how the 5300 Fragment Agilent works, you can view and download the Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide on the Manualsnet website.

Yes, we have the Quick Guide for Agilent 5300 Fragment as well as other Agilent manuals. All you need to do is to use our search bar and find the user manual that you are looking for.

The Quick Guide should include all the details that are needed to use a Agilent 5300 Fragment. Full manuals and user guide PDFs can be downloaded from Manualsnet.com.

The best way to navigate the Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide is by checking the Table of Contents at the top of the page where available. This allows you to navigate a manual by jumping to the section you are looking for.

This Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide consists of sections like Table of Contents, to name a few. For easier navigation, use the Table of Contents in the upper left corner.

You can download Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide free of charge simply by clicking the “download” button in the upper right corner of any manuals page. This feature allows you to download any manual in a couple of seconds and is generally in PDF format. You can also save a manual for later by adding it to your saved documents in the user profile.

To be able to print Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide, simply download the document to your computer. Once downloaded, open the PDF file and print the Agilent 5300 Fragment 96-Capillary Arrays Analyzer Quick Guide as you would any other document. This can usually be achieved by clicking on “File” and then “Print” from the menu bar.